DK1077262T3 - Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner - Google Patents

Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner

Info

Publication number
DK1077262T3
DK1077262T3 DK00115839T DK00115839T DK1077262T3 DK 1077262 T3 DK1077262 T3 DK 1077262T3 DK 00115839 T DK00115839 T DK 00115839T DK 00115839 T DK00115839 T DK 00115839T DK 1077262 T3 DK1077262 T3 DK 1077262T3
Authority
DK
Denmark
Prior art keywords
secretion
preparation
periplasm
naturally folded
secreted proteins
Prior art date
Application number
DK00115839T
Other languages
Danish (da)
English (en)
Inventor
Dorothee Ambrosius
Rainer Rudolph
Joerg Schaeffner
Elisabeth Schwarz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1077262T3 publication Critical patent/DK1077262T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DK00115839T 1999-07-29 2000-07-24 Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner DK1077262T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP99114811A EP1077263A1 (fr) 1999-07-29 1999-07-29 Procédé de préparation des protéines secrétées sous une forme pliée naturelle par co-sécrétion de chaperones

Publications (1)

Publication Number Publication Date
DK1077262T3 true DK1077262T3 (da) 2005-11-07

Family

ID=8238680

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00115839T DK1077262T3 (da) 1999-07-29 2000-07-24 Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner

Country Status (13)

Country Link
US (1) US6455279B1 (fr)
EP (2) EP1077263A1 (fr)
JP (1) JP2001061487A (fr)
KR (1) KR100378325B1 (fr)
CN (1) CN1231595C (fr)
AT (1) ATE299942T1 (fr)
CA (1) CA2313248C (fr)
DE (1) DE60021318T2 (fr)
DK (1) DK1077262T3 (fr)
ES (1) ES2247990T3 (fr)
IN (1) IN190327B (fr)
JO (1) JO2309B1 (fr)
MX (1) MXPA00007339A (fr)

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US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
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MXPA03011455A (es) * 2001-06-22 2004-04-05 Hoffmann La Roche Complejo soluble que comprende glucoproteina de superficie retroviral.
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
US7094884B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them
KR100441755B1 (ko) * 2001-07-24 2004-08-02 재단법인서울대학교산학협력재단 분자 샤페론을 핵 내부로 이동시키는 방법
CN100513414C (zh) * 2001-08-27 2009-07-15 杰南技术公司 进行抗体表达和装配的系统
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
WO2004042017A2 (fr) 2002-10-31 2004-05-21 Genentech, Inc. Methodes et compositions pouvant augmenter la production d'anticorps
EP1585768A2 (fr) * 2003-01-23 2005-10-19 Genentech, Inc. Procedes de production d'anticoprs humanises et d'amelioration du rendement d'anticorps ou de fragments de liaison d'antigenes en culture cellulaire
EP1624886A2 (fr) * 2003-05-12 2006-02-15 Expressive Constructs, Inc. Procedes d'augmentation de la viabilite de cellules et de tissus
AU2004273791A1 (en) * 2003-09-05 2005-03-31 Genentech, Inc. Antibodies with altered effector functions
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
US9057061B2 (en) * 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
WO2006028936A2 (fr) * 2004-09-02 2006-03-16 Genentech, Inc. Molecules heteromultimeriques
GB2440490B (en) 2005-05-23 2010-06-02 Korea Advanced Inst Sci & Tech Method for preparing a target protein using the sHSPs
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
DK2102239T3 (da) 2006-11-30 2012-05-29 Res Dev Foundation Forbedrede immunoglobulin-biblioteker
CN101294164B (zh) * 2007-04-23 2013-01-16 南京大学 重组人中期因子(rh-Midkine)的表达及其单克隆抗体的制备和应用
EP2155789B1 (fr) * 2007-05-01 2013-07-24 Research Development Foundation Banques d'immunoglobulines fc
CN101307310B (zh) * 2007-05-15 2012-08-08 康泰生医科技股份有限公司 具有提高产率及免疫原性的重组蛋白质表达系统
CN102549016B (zh) * 2009-06-30 2015-05-06 研究发展基金会 免疫球蛋白fc多肽
US8952132B2 (en) 2011-02-07 2015-02-10 Research Development Foundation Engineered immunoglobulin FC polypeptides
WO2014054744A1 (fr) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 Procédé de prévention de la réduction de polypeptide par l'ajout d'acide aminé à un milieu de culture liquide
US9758807B2 (en) 2012-12-05 2017-09-12 SOLA Biosciences, LLC Protein expression enhancing polypeptides
WO2016130516A1 (fr) 2015-02-09 2016-08-18 Research Development Foundation Polypeptides ayant un domaine fc d'immunoglobuline modifié manifestant une activation du complément améliorée
CN105296652B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp40基因表达的方法
CN105296654B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp60基因表达的方法
CN105296651B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp90基因表达的方法
CN105296653B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp83基因表达的方法
CN110734480B (zh) * 2019-11-07 2021-08-24 中国科学院遗传与发育生物学研究所 大肠杆菌分子伴侣GroEL/ES在协助合成植物Rubisco中的应用
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins
CN112239760B (zh) * 2020-09-18 2022-02-11 深圳科兴药业有限公司 高效表达重组hGH的重组工程菌及构建方法和应用
CN112940955B (zh) * 2021-04-18 2022-10-18 江南大学 一种高效合成乳铁蛋白的毕赤酵母及其构建方法与应用

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Also Published As

Publication number Publication date
CA2313248C (fr) 2010-05-25
DE60021318D1 (de) 2005-08-25
DE60021318T2 (de) 2006-05-24
EP1077263A1 (fr) 2001-02-21
JP2001061487A (ja) 2001-03-13
KR100378325B1 (ko) 2003-03-29
EP1077262B1 (fr) 2005-07-20
ATE299942T1 (de) 2005-08-15
MXPA00007339A (es) 2002-08-06
ES2247990T3 (es) 2006-03-16
JO2309B1 (en) 2005-09-12
CN1283702A (zh) 2001-02-14
IN190327B (fr) 2003-07-19
KR20010049895A (ko) 2001-06-15
US6455279B1 (en) 2002-09-24
CN1231595C (zh) 2005-12-14
EP1077262A1 (fr) 2001-02-21
CA2313248A1 (fr) 2001-01-29

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