DK1104457T3 - Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser - Google Patents

Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser

Info

Publication number
DK1104457T3
DK1104457T3 DK99936725T DK99936725T DK1104457T3 DK 1104457 T3 DK1104457 T3 DK 1104457T3 DK 99936725 T DK99936725 T DK 99936725T DK 99936725 T DK99936725 T DK 99936725T DK 1104457 T3 DK1104457 T3 DK 1104457T3
Authority
DK
Denmark
Prior art keywords
sequences
library
polynucleotide sequences
recombined
sequence
Prior art date
Application number
DK99936725T
Other languages
Danish (da)
English (en)
Inventor
Daniel Dupret
Jean-Michel Masson
Fabrice Lefevre
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Application granted granted Critical
Publication of DK1104457T3 publication Critical patent/DK1104457T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK99936725T 1998-08-12 1999-08-11 Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser DK1104457T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9810338A FR2782323B1 (fr) 1998-08-12 1998-08-12 Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues

Publications (1)

Publication Number Publication Date
DK1104457T3 true DK1104457T3 (da) 2006-03-27

Family

ID=9529621

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99936725T DK1104457T3 (da) 1998-08-12 1999-08-11 Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser

Country Status (12)

Country Link
EP (2) EP1104457B1 (de)
JP (1) JP4448619B2 (de)
AT (1) ATE309342T1 (de)
AU (1) AU769516C (de)
CA (1) CA2339901C (de)
DE (1) DE69928263T2 (de)
DK (1) DK1104457T3 (de)
ES (1) ES2253901T3 (de)
FR (1) FR2782323B1 (de)
IL (2) IL141373A0 (de)
NO (1) NO20015787L (de)
WO (1) WO2000009679A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
WO2001029211A2 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
WO2001096551A2 (en) * 2000-06-14 2001-12-20 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
JP4533584B2 (ja) * 2001-04-25 2010-09-01 プロテウス ポリヌクレオチドを非ランダム的にシャッフルする、テンプレート調整ライゲーション方向定位の方法
FR2824073B1 (fr) * 2001-04-25 2003-12-19 Proteus Procede de creation in vitro de sequences polynucleotidiques recombinees par ligation orientee
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
DE60210298T2 (de) * 2001-07-23 2006-11-02 Dsm Ip Assets B.V. Verfahren zur herstellung von polynucleotidvarianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
EP1493027B1 (de) 2002-03-01 2014-10-15 Codexis Mayflower Holdings, LLC Verfahren, systeme und software zur identifizierung funktioneller biomoleküle
US7620500B2 (en) 2002-03-09 2009-11-17 Maxygen, Inc. Optimization of crossover points for directed evolution
AU2003243157C1 (en) 2002-04-19 2008-09-04 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
WO2005003289A2 (en) 2002-08-06 2005-01-13 Verdia, Inc. Ap1 amine oxidase variants
DK2194133T3 (en) 2003-03-06 2016-02-29 Basf Enzymes Llc Amylases, nucleic acids encoding them, and methods of making and using the same
EP2853593B1 (de) 2003-03-07 2017-10-04 DSM IP Assets B.V. Hydrolasen, Nucleinsäuren zur Codierung davon sowie Verfahren zur Herstellung und Verwendung davon
US7592434B2 (en) 2003-04-04 2009-09-22 Verenium Corporation Pectate lyases, nucleic encoding them and methods for making and using them
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
CN103484486B (zh) 2003-07-02 2018-04-24 维莱尼姆公司 葡聚糖酶，编码它们的核酸以及制备和使用它们的方法
EP1668113B1 (de) 2003-08-11 2013-06-19 Verenium Corporation Laccasen, diese codierende nukleinsäuren und verfahren zur herstellung und verwendung davon
WO2005017116A2 (en) 2003-08-11 2005-02-24 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
AU2005254993B2 (en) 2004-06-09 2009-08-06 Pioneer Hi-Bred International, Inc. Plastid transit peptides
CN101432292B (zh) 2004-06-16 2013-03-13 维莱尼姆公司 对叶绿素进行酶促脱色的组合物和方法
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
EP2886658A1 (de) 2005-03-10 2015-06-24 BASF Enzymes LLC Lyaseenzyme, Nukleinsäuren zur Codierung davon und Verfahren zur Herstellung und Verwendung davon
MX300732B (es) 2005-03-15 2012-06-28 Verenium Corp Celulasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
WO2007094852A2 (en) 2006-02-10 2007-08-23 Verenium Corporation Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP2548955A1 (de) 2006-02-14 2013-01-23 Verenium Corporation Xylanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
BRPI0708614A2 (pt) 2006-03-07 2011-06-07 Verenium Corp aldolases, ácidos nucléicos codificando as mesmas e métodos para produzir e usar as mesmas
MX2008011477A (es) 2006-03-07 2008-09-23 Cargill Inc Aldolasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
EP1842915A1 (de) 2006-04-04 2007-10-10 Libragen In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen
EP2069513A4 (de) 2006-08-04 2012-03-21 California Inst Of Techn Verfahren und systeme zur selektiven fluorinierung organischer moleküle
BRPI0714876B1 (pt) 2006-08-04 2022-04-19 Verenium Corporation Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
AR062947A1 (es) 2006-09-21 2008-12-17 Verenium Corp Fosfolipasas acidos nucleicos que las codifican y metodos para prepararlas y usarlas
JP5307013B2 (ja) 2006-09-21 2013-10-02 ヴェレニウム コーポレイション フィターゼ、フィターゼをコードする核酸、並びにフィターゼを製造および使用する方法
PL2069490T5 (pl) 2006-12-21 2018-09-28 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
NZ578309A (en) 2007-01-30 2012-06-29 Verenium Corp Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
US8501452B2 (en) 2007-06-28 2013-08-06 Firmenich, Sa Modified 13-hydroperoxide lyases and uses thereof
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
WO2009045627A2 (en) 2007-10-03 2009-04-09 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
DK2238261T3 (da) 2008-01-03 2014-02-10 Verenium Corp Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
WO2009143397A2 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CA2738472A1 (en) 2008-09-26 2010-04-22 Tocagen Inc. Gene therapy vectors and cytosine deaminases
WO2010135588A2 (en) 2009-05-21 2010-11-25 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
CN103237894A (zh) 2010-08-13 2013-08-07 先锋国际良种公司 包含具有羟基苯丙酮酸双加氧酶(hppd)活性的序列的组合物和方法
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
JP6745599B2 (ja) 2012-12-06 2020-08-26 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 分子の作製
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
MX359026B (es) 2013-08-16 2018-09-12 Pioneer Hi Bred Int Proteinas insecticidas y metodos de uso.
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
FR3013731B1 (fr) 2013-11-22 2017-09-01 Ifp Energies Now Variants d'endoglucanases a activite amelioree et leurs utilisations
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
CA3290904A1 (en) 2014-02-07 2026-03-02 E. I. Du Pont De Nemours And Company Insecticidal proteins and methods for their use
FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
FR3022557B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CA2963558C (en) 2014-10-16 2023-04-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN107635396B (zh) 2015-01-15 2021-12-24 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA3268091A1 (en) 2015-08-06 2026-03-02 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX387077B (es) 2016-05-04 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
CA3026113A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
EP3555118B1 (de) 2016-12-14 2021-08-18 Pioneer Hi-Bred International Inc. Insektizide proteine und verfahren zu deren verwendung
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
MX2019013321A (es) 2017-05-11 2020-02-10 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
CN111867377B (zh) 2018-03-14 2023-05-23 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
AU2019234566B2 (en) 2018-03-14 2024-09-26 Hexima Limited Insecticidal proteins from plants and methods for their use
MX2022008463A (es) 2020-01-10 2022-10-18 Univ California Plataforma biosintética para la producción de ácido olivetólico y análogos del ácido olivetólico.
US12168774B2 (en) 2020-07-14 2024-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP3018163B2 (ja) * 1997-09-04 2000-03-13 工業技術院長 パイロコッカス属に属する超好熱性細菌由来の耐熱性Ｆｌａｐエンドヌクレアーゼ

Also Published As

Publication number Publication date
JP4448619B2 (ja) 2010-04-14
CA2339901C (fr) 2012-02-28
IL141373A0 (en) 2002-03-10
DE69928263D1 (de) 2005-12-15
EP1683861A1 (de) 2006-07-26
ATE309342T1 (de) 2005-11-15
NO20015787D0 (no) 2001-11-27
AU5171799A (en) 2000-03-06
DE69928263T2 (de) 2006-08-10
FR2782323A1 (fr) 2000-02-18
NO20015787L (no) 2001-11-27
ES2253901T3 (es) 2006-06-01
FR2782323B1 (fr) 2002-01-11
CA2339901A1 (fr) 2000-02-24
AU769516C (en) 2004-11-18
EP1104457A1 (de) 2001-06-06
JP2002538763A (ja) 2002-11-19
AU769516B2 (en) 2004-01-29
WO2000009679A1 (fr) 2000-02-24
IL141373A (en) 2008-11-03
EP1104457B1 (de) 2005-11-09

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