DK1218542T3 - Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser - Google Patents

Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser

Info

Publication number
DK1218542T3
DK1218542T3 DK00963419T DK00963419T DK1218542T3 DK 1218542 T3 DK1218542 T3 DK 1218542T3 DK 00963419 T DK00963419 T DK 00963419T DK 00963419 T DK00963419 T DK 00963419T DK 1218542 T3 DK1218542 T3 DK 1218542T3
Authority
DK
Denmark
Prior art keywords
methods
primer
composite
compositions
polynucleotide sequences
Prior art date
Application number
DK00963419T
Other languages
English (en)
Inventor
Nurith Kurn
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Application granted granted Critical
Publication of DK1218542T3 publication Critical patent/DK1218542T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Amplifiers (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK00963419T 1999-09-13 2000-09-13 Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser DK1218542T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15360499P 1999-09-13 1999-09-13
US17578000P 2000-01-12 2000-01-12

Publications (1)

Publication Number Publication Date
DK1218542T3 true DK1218542T3 (da) 2004-08-02

Family

ID=26850696

Family Applications (2)

Application Number Title Priority Date Filing Date
DK04002084.4T DK1431303T3 (da) 1999-09-13 2000-09-13 Sammensætning til lineær isotermisk amplifikation af polynukleotidsekvenser
DK00963419T DK1218542T3 (da) 1999-09-13 2000-09-13 Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK04002084.4T DK1431303T3 (da) 1999-09-13 2000-09-13 Sammensætning til lineær isotermisk amplifikation af polynukleotidsekvenser

Country Status (19)

Country Link
US (2) US6251639B1 (da)
EP (2) EP1431303B1 (da)
JP (2) JP3929775B2 (da)
KR (1) KR100527265B1 (da)
CN (2) CN1373812B (da)
AT (1) ATE262595T1 (da)
AU (1) AU783873B2 (da)
BR (1) BR0014182A (da)
CA (1) CA2384838C (da)
DE (1) DE60009323T2 (da)
DK (2) DK1431303T3 (da)
ES (2) ES2447419T3 (da)
HK (1) HK1046021B (da)
IL (1) IL148091A0 (da)
MX (1) MXPA02002656A (da)
NO (1) NO20021223L (da)
NZ (1) NZ517121A (da)
PT (1) PT1218542E (da)
WO (1) WO2001020035A2 (da)

Families Citing this family (366)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP2360270B1 (en) 1999-05-20 2016-11-09 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
ATE542916T1 (de) * 1999-08-18 2012-02-15 Illumina Inc Methoden zur erzeugung von oligonukleotidlösungen
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
AU3806701A (en) 2000-02-07 2001-08-14 Illumina Inc Nucleic acid detection methods using universal priming
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
AU2001238068A1 (en) * 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
AU2001239760B2 (en) * 2000-02-10 2005-11-24 Illumina, Inc. Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
ATE412774T1 (de) * 2000-02-16 2008-11-15 Illumina Inc Parallele genotypisierung mehrerer patientenproben
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
ATE384072T1 (de) * 2000-09-26 2008-02-15 Idera Pharmaceuticals Inc Modulation der immunostimulatorischen aktivität von immunostimulierenden oligonukleotidanaloga durch positionelle chemische veränderungen
CA2423729A1 (en) 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
WO2002048401A2 (en) 2000-12-11 2002-06-20 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
WO2002048402A2 (en) * 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
JPWO2002062993A1 (ja) * 2001-02-06 2004-06-10 タカラバイオ株式会社 増幅核酸及びその固定化物
KR20030082535A (ko) * 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Rna 서열의 증폭을 위한 방법 및 조성물
CA2439074A1 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
BR0209700A (pt) 2001-06-07 2004-10-13 Pioneer Hi Bred Int Resistência à produção de sclerotinia por controle de qtl em soja
EA006679B1 (ru) * 2001-06-12 2006-02-24 Такара Био Инк. Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
CA2453527A1 (en) 2001-07-12 2003-01-23 Illumina, Inc. Multiplex nucleic acid reactions
JPWO2003016569A1 (ja) * 2001-08-20 2004-12-02 タカラバイオ株式会社 核酸の増幅方法
AU2005243741B2 (en) * 2001-09-18 2010-01-28 Bioexpertise Llc Methods for delivering MBD peptide- linked agent into cells under conditions of cellular stress
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
ATE448320T1 (de) * 2001-09-19 2009-11-15 Alexion Pharma Inc Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation
DE10151069A1 (de) 2001-10-05 2003-04-30 Epigenomics Ag Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga
WO2003069333A1 (en) 2002-02-14 2003-08-21 Illumina, Inc. Automated information processing in randomly ordered arrays
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
NZ535044A (en) * 2002-03-01 2008-12-24 Ravgen Inc Non-invasive method to determine the genetic sequence of foetal DNA from a sample from a pregnant female thereby detecting any alternation in gene sequence as compared with the wild type sequence
US7112406B2 (en) * 2002-03-01 2006-09-26 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
AU2003222269A1 (en) * 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
CA2480525A1 (en) * 2002-03-29 2003-10-09 Nugen Technologies, Inc. Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
WO2004009765A2 (en) 2002-07-19 2004-01-29 Althea Technologies, Inc. Strategies for gene expression analysis
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
EP1549764B1 (en) 2002-10-11 2010-10-06 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for pcr-based clonality studies
US7413857B2 (en) * 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US20040115643A1 (en) * 2002-12-12 2004-06-17 Lizardi Paul M. Thermodynamic equilibrium extension of primers
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
CA2513985C (en) * 2003-01-21 2012-05-29 Illumina Inc. Chemical reaction monitor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
CA2897376A1 (en) 2003-02-26 2004-09-10 Radoje T. Drmanac Modular system and probes for dna analysis
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ, インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
JP2006525023A (ja) * 2003-04-29 2006-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸配列の多重開始増幅
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
DK1664343T3 (da) 2003-09-09 2014-08-11 Integrigen Inc Fremgangsmåder og sammensætninger til generering af kimcelle humane antistofgener
ATE461292T1 (de) * 2003-09-10 2010-04-15 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
US20060094064A1 (en) * 2003-11-19 2006-05-04 Sandip Ray Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids
PT2211183E (pt) * 2003-11-19 2013-05-31 Satoris Inc Método para diagnosticar e monitorizar a doença de alzheimer
US7344834B2 (en) * 2003-12-08 2008-03-18 Cytyc Corporation Method for DNA amplification using DNA blocking probes
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
KR100590621B1 (ko) * 2004-09-21 2006-06-19 (주)지노믹트리 하이힐 프라이머를 이용한 rna의 선형 증폭방법
WO2006033487A1 (en) * 2004-09-21 2006-03-30 Genomictree Inc. Method for linear amplification of rna using high-heel primer
WO2006086668A2 (en) * 2005-02-09 2006-08-17 Epicentre Technologies Compositions and methods employing 5'-phosphate-dependent nucleic acid exonucleases
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US20060234268A1 (en) * 2005-04-15 2006-10-19 Sungwhan An Linear amplification of rna
KR101381331B1 (ko) 2005-05-09 2014-04-04 테라노스, 인코포레이티드 현장진료 유체 시스템 및 그 용도
US20090317798A1 (en) 2005-06-02 2009-12-24 Heid Christian A Analysis using microfluidic partitioning devices
US20070020639A1 (en) * 2005-07-20 2007-01-25 Affymetrix, Inc. Isothermal locus specific amplification
EP1929045B9 (en) * 2005-09-06 2012-06-20 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US20070065814A1 (en) * 2005-09-21 2007-03-22 Engelhard Eric K Detecting foot-and-mouth disease virus
US7485425B2 (en) * 2005-10-03 2009-02-03 Applied Biosystems Inc. Methods for amplification of nucleic acids using spanning primers
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US8831887B2 (en) * 2005-10-12 2014-09-09 The Research Foundation For The State University Of New York Absolute PCR quantification
KR100816419B1 (ko) 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
US8609829B2 (en) * 2005-10-17 2013-12-17 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
US7611893B2 (en) 2005-11-09 2009-11-03 Ontherix, Inc. Metal-binding therapeutic peptides
US7662624B2 (en) * 2005-11-09 2010-02-16 Ontherix, Inc. Metal-binding therapeutic peptides
US20080039393A1 (en) * 2005-11-09 2008-02-14 Desmond Mascarenhas Metal-binding therapeutic peptides
AU2006311538A1 (en) * 2005-11-09 2007-05-18 Ontherix, Inc. Metal-binding therapeutic peptides
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
CA2650820C (en) 2006-04-28 2014-09-16 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
WO2007127999A2 (en) * 2006-04-28 2007-11-08 Igor Kutyavin Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection
EP2079838A1 (en) * 2006-05-16 2009-07-22 NuGEN Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2038429B1 (en) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
CN101495657B (zh) * 2006-07-31 2016-08-10 毕万里 使用可逆修饰寡核苷酸扩增核酸
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JPWO2008041354A1 (ja) * 2006-10-03 2010-02-04 国立大学法人岐阜大学 DnaJ遺伝子を利用した細菌の検出及びその利用
US8338109B2 (en) 2006-11-02 2012-12-25 Mayo Foundation For Medical Education And Research Predicting cancer outcome
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2673017C (en) 2006-12-21 2015-08-04 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
JP5194459B2 (ja) * 2007-01-25 2013-05-08 ソニー株式会社 一本鎖dna増幅方法
US8753833B2 (en) * 2007-06-21 2014-06-17 Momenta Pharmaceuticals, Inc. Copolymer assay
EP2162551B1 (en) * 2007-07-03 2017-08-23 Genaphora LTD. Chimeric primers for improved nucleic acid amplification reactions
US8143006B2 (en) * 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US8088593B2 (en) 2007-10-02 2012-01-03 Theranos, Inc. Modular point-of-care devices, systems, and uses thereof
KR100957057B1 (ko) * 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US8536135B2 (en) * 2008-03-19 2013-09-17 Ontherix, Inc. Adaptive biochemical signatures
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644710B1 (en) 2008-04-30 2016-12-21 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
WO2009137369A1 (en) * 2008-05-03 2009-11-12 Tufts Medical Center, Inc. Neonatal salivary genomics
CA2725978A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
CN102119225B (zh) * 2008-06-11 2015-04-08 基因排列技术有限公司 等温核酸扩增
US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
CA2641132A1 (en) * 2008-10-03 2010-04-03 Richard T. Scott, Jr. Improvements in in vitro fertilization
US20100124756A1 (en) * 2008-10-10 2010-05-20 Sandip Ray Collection of biomarkers for diagnosis and monitoring of alzheimer's disease in body fluids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2743029T3 (es) 2008-10-24 2020-02-18 Epicentre Tech Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US20100206316A1 (en) * 2009-01-21 2010-08-19 Scott Jr Richard T Method for determining chromosomal defects in an ivf embryo
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
US8669057B2 (en) 2009-05-07 2014-03-11 Veracyte, Inc. Methods and compositions for diagnosis of thyroid conditions
EP2432899A1 (en) 2009-05-22 2012-03-28 Population Genetics Technologies LTD. Sorting asymmetrically tagged nucleic acids by selective primer extension
US20100317916A1 (en) * 2009-06-12 2010-12-16 Scott Jr Richard T Method for relative quantitation of chromosomal DNA copy number in single or few cells
AU2010266234B2 (en) 2009-07-01 2013-07-25 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US20110189679A1 (en) * 2009-09-11 2011-08-04 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
EP2483425B1 (en) 2009-09-28 2016-08-24 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
EP2494066B1 (en) * 2009-10-27 2017-04-05 Swift Biosciences, Inc. Bimolecular primers
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2529026B1 (en) 2010-01-25 2013-11-13 Rd Biosciences Inc. Self-folding amplification of target nucleic acid
US20110202284A1 (en) * 2010-02-10 2011-08-18 Mcreynolds Cristopher Novel groups of biomarkers for diagnosing alzheimer's disease
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
US20130267443A1 (en) 2010-11-19 2013-10-10 The Regents Of The University Of Michigan ncRNA AND USES THEREOF
CN106323875B (zh) 2011-01-21 2019-07-09 西拉诺斯知识产权有限责任公司 样品使用最大化的系统和方法
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2705162B1 (en) * 2011-05-04 2018-07-11 Biocept, Inc. Methods for detecting nucleic acid sequence variants
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
MX344792B (es) 2011-09-25 2017-01-06 Theranos Inc Sistemas y métodos para múltiples análisis.
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
US10513737B2 (en) 2011-12-13 2019-12-24 Decipher Biosciences, Inc. Cancer diagnostics using non-coding transcripts
US9422601B2 (en) 2011-12-15 2016-08-23 Samsung Electronics Co., Ltd. Method of determining a ratio of RNA species in a sample
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CN104379563B (zh) 2012-04-10 2018-12-21 加利福尼亚大学董事会 用于治疗癌症的组合物和方法
EP2847353B1 (en) 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ, インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
ES3015226T3 (en) 2012-08-16 2025-04-30 Veracyte Sd Inc Prostate cancer prognosis using biomarkers
AU2013315800A1 (en) 2012-09-11 2015-03-12 Theranos Ip Company, Llc Information management systems and methods using a biological signature
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9410173B2 (en) * 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
CA2892818A1 (en) 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
US20150299772A1 (en) * 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
EP2925893A4 (en) * 2012-12-03 2016-09-07 Elim Biopharmaceuticals Inc COMPOSITIONS AND METHODS FOR THE PREPARATION OF NUCLEIC ACID AND ANALYZES
SG11201506421PA (en) 2013-02-18 2015-09-29 Theranos Inc Systems and methods for collecting and transmitting assay results
US9227978B2 (en) 2013-03-15 2016-01-05 Araxes Pharma Llc Covalent inhibitors of Kras G12C
WO2015076919A1 (en) * 2013-11-22 2015-05-28 Theranos, Inc. Nucleic Acid Amplification
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
CA2908877A1 (en) 2013-04-25 2014-10-30 Orion Diagnostica Oy Strand-invasion based dna amplification method
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
US10392670B2 (en) 2013-07-25 2019-08-27 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
EP3715470B1 (en) 2013-08-09 2023-10-11 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US9410172B2 (en) 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
JO3805B1 (ar) 2013-10-10 2021-01-31 Araxes Pharma Llc مثبطات كراس جي12سي
CA2923812C (en) 2013-10-17 2023-10-17 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
EP3077543B1 (en) 2013-12-05 2019-09-25 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR20240025725A (ko) 2013-12-11 2024-02-27 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
EP3105349B1 (en) * 2014-02-11 2020-07-15 F. Hoffmann-La Roche AG Targeted sequencing and uid filtering
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
JO3556B1 (ar) 2014-09-18 2020-07-05 Araxes Pharma Llc علاجات مدمجة لمعالجة السرطان
JP2017528498A (ja) 2014-09-25 2017-09-28 アラクセス ファーマ エルエルシー Kras g12c変異体タンパク質のインヒビター
WO2016049568A1 (en) 2014-09-25 2016-03-31 Araxes Pharma Llc Methods and compositions for inhibition of ras
CN114606309A (zh) 2014-11-05 2022-06-10 威拉赛特公司 使用机器学习和高维转录数据的诊断系统和方法
CN107109495B (zh) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
US10760182B2 (en) * 2014-12-16 2020-09-01 Apdn (B.V.I.) Inc. Method and device for marking fibrous materials
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
MX382355B (es) 2015-04-10 2025-03-13 Araxes Pharma Llc Compuestos de quinazolina sustituidos y métodos de uso de los mismos.
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
EP3283462B1 (en) 2015-04-15 2020-12-02 Araxes Pharma LLC Fused-tricyclic inhibitors of kras and methods of use thereof
EP4220645A3 (en) 2015-05-14 2023-11-08 Life Technologies Corporation Barcode sequences, and related systems and methods
WO2016201111A1 (en) 2015-06-09 2016-12-15 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10144724B2 (en) 2015-07-22 2018-12-04 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017050803A1 (en) 2015-09-25 2017-03-30 Universität Duisburg-Essen Agents inhibiting kallikrein-8 for use in the prevention or treatment of alzheimer's disease
EP3356354A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3356349A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
US10689356B2 (en) 2015-09-28 2020-06-23 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356351A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
WO2017058792A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
WO2017058728A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
EP3356347A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
MX2018004284A (es) 2015-10-09 2018-05-16 Accuragen Holdings Ltd Metodos y composiciones para el enriquecimiento de productos de amplificacion.
WO2017070256A2 (en) 2015-10-19 2017-04-27 Araxes Pharma Llc Method for screening inhibitors of ras
EP3377481A1 (en) 2015-11-16 2018-09-26 Araxes Pharma LLC 2-substituted quinazoline compounds comprising a substituted heterocyclic group and methods of use thereof
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
WO2017100546A1 (en) 2015-12-09 2017-06-15 Araxes Pharma Llc Methods for preparation of quinazoline derivatives
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US10822312B2 (en) 2016-03-30 2020-11-03 Araxes Pharma Llc Substituted quinazoline compounds and methods of use
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
EP3455372B1 (en) 2016-05-11 2019-10-30 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP4257701A3 (en) 2016-06-30 2023-12-20 Grail, LLC Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
US10646488B2 (en) 2016-07-13 2020-05-12 Araxes Pharma Llc Conjugates of cereblon binding compounds and G12C mutant KRAS, HRAS or NRAS protein modulating compounds and methods of use thereof
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11414708B2 (en) 2016-08-24 2022-08-16 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
JP6997773B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス, エルエルシー 無細胞dnaの分析用の核酸サンプル調製の方法
EP3519402A1 (en) 2016-09-29 2019-08-07 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3519554A4 (en) 2016-09-30 2020-05-20 The Governing Council Of The University Of Toronto System for identifying and targeting individual cells within a heterogeneous population for selective extraction of cellular content
US10377743B2 (en) 2016-10-07 2019-08-13 Araxes Pharma Llc Inhibitors of RAS and methods of use thereof
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
EP3555077B1 (en) 2016-12-15 2023-04-19 The Regents of The University of California Compositions and methods for treating cancer
GB201621477D0 (en) * 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
EP3565907B1 (en) 2017-01-06 2022-05-04 Editas Medicine, Inc. Methods of assessing nuclease cleavage
AU2018210695B2 (en) 2017-01-20 2024-07-18 The University Of British Columbia Molecular subtyping, prognosis, and treatment of bladder cancer
EP3573954A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC Fused bicyclic benzoheteroaromatic compounds and methods of use thereof
US11358959B2 (en) 2017-01-26 2022-06-14 Araxes Pharma Llc Benzothiophene and benzothiazole compounds and methods of use thereof
US11136308B2 (en) 2017-01-26 2021-10-05 Araxes Pharma Llc Substituted quinazoline and quinazolinone compounds and methods of use thereof
WO2018140513A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc 1-(3-(6-(3-hydroxynaphthalen-1-yl)benzofuran-2-yl)azetidin-1yl)prop-2-en-1-one derivatives and similar compounds as kras g12c modulators for treating cancer
CN110382482A (zh) 2017-01-26 2019-10-25 亚瑞克西斯制药公司 稠合的杂-杂二环化合物及其使用方法
CN110382708A (zh) * 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
WO2018165600A1 (en) 2017-03-09 2018-09-13 Genomedx Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11078542B2 (en) 2017-05-12 2021-08-03 Decipher Biosciences, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor aggressiveness
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたdnaライブラリーの作製方法
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
CN110869358A (zh) 2017-05-25 2020-03-06 亚瑞克西斯制药公司 Kras的共价抑制剂
JP2020521741A (ja) 2017-05-25 2020-07-27 アラクセス ファーマ エルエルシー がんの処置のための化合物およびその使用の方法
CN110831933A (zh) 2017-05-25 2020-02-21 亚瑞克西斯制药公司 喹唑啉衍生物作为突变kras、hras或nras的调节剂
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
AU2018310987A1 (en) 2017-08-04 2020-02-27 Decipher Biosciences, Inc. Use of immune cell-specific gene expression for prognosis of prostate cancer and prediction of responsiveness to radiation therapy
US11667952B2 (en) 2017-08-24 2023-06-06 Takara Bio Usa, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US10724017B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
IL276343B2 (en) * 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
FI3827011T3 (fi) 2018-07-24 2024-09-09 Salish Bioscience Inc Menetelmiä ja koostumus kohdennettua genomianalyysiä varten
BR112021001709A2 (pt) 2018-08-01 2021-05-04 Araxes Pharma Llc compostos espiro heterocíclicos e métodos de uso dos mesmos para o tratamento de câncer
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12421519B2 (en) 2019-01-07 2025-09-23 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
CN120099139A (zh) 2019-02-14 2025-06-06 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
JP7687957B2 (ja) 2019-03-14 2025-06-03 インシリクサ, インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4081777B1 (en) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
EP4121555B1 (en) 2020-12-21 2026-02-25 10X Genomics, Inc. Methods for capturing probes and/or barcodes
CN112852929A (zh) * 2021-02-08 2021-05-28 广州普世利华科技有限公司 用于检测dna的组合产品
BR112023018157A2 (pt) 2021-03-09 2023-10-31 Illumina Inc Análise de expressão de variantes codificantes de proteína em células
KR20230154078A (ko) 2021-03-09 2023-11-07 일루미나, 인코포레이티드 CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
ES3035187T3 (en) 2021-08-11 2025-08-29 Illumina Inc Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
CN113981041B (zh) * 2021-11-25 2024-04-30 首都医科大学附属北京安贞医院 靶向富集测序试剂及靶向富集方法
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
JP2025083598A (ja) * 2022-03-31 2025-06-02 国立大学法人埼玉大学 Rna/dnaキメラプライマーを用いたpcr及びその増幅断片連結方法
KR102880701B1 (ko) * 2022-10-04 2025-11-04 건국대학교 산학협력단 CRISPR/Cas12a의 트랜스-절단 활성을 이용한 표적 핵산 검출용 조성물, 키트 및 검출 방법
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2025255512A1 (en) 2024-06-07 2025-12-11 Juno Therapeutics, Inc. Grna sequencing compositions and methods

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57501692A (da) 1980-09-24 1982-09-16
ATE53862T1 (de) 1982-01-22 1990-06-15 Cetus Corp Verfahren zur charakterisierung von hla und die darin benutzten cdns-testmittel.
US4582788A (en) 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
DE3634356A1 (de) 1986-10-08 1988-04-21 Epis Sa Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US6004745A (en) 1987-09-21 1999-12-21 Gen-Probe Incorporated Hybridization protection assay
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5766849A (en) 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5480784A (en) 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5527872A (en) 1990-09-14 1996-06-18 At&T Global Information Solutions Company Electronic device with a spin-on glass dielectric layer
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5169766A (en) 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
ATE152180T1 (de) 1992-07-31 1997-05-15 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
JPH0723799A (ja) * 1993-07-13 1995-01-27 Hitachi Ltd ポリヌクレオチドの検出方法
US6027923A (en) 1993-07-23 2000-02-22 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2724934B1 (fr) 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
EP0866868B1 (en) 1995-12-15 2001-07-11 Amersham Life Science Inc Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
DE19653439A1 (de) 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
WO1999029901A1 (en) * 1997-12-11 1999-06-17 The General Hospital Corporation Broad range pcr amplification techniques
JP4317953B2 (ja) * 1998-01-22 2009-08-19 独立行政法人理化学研究所 Dnaの塩基配列決定方法
EP1055001A1 (en) * 1998-02-05 2000-11-29 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6410278B1 (en) 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
WO2000070095A2 (en) * 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide

Also Published As

Publication number Publication date
CN102586228A (zh) 2012-07-18
US20010034048A1 (en) 2001-10-25
IL148091A0 (en) 2002-09-12
WO2001020035A3 (en) 2001-12-06
CN1373812A (zh) 2002-10-09
EP1218542A2 (en) 2002-07-03
CA2384838C (en) 2006-07-18
WO2001020035A2 (en) 2001-03-22
PT1218542E (pt) 2004-08-31
NZ517121A (en) 2004-05-28
US6251639B1 (en) 2001-06-26
EP1431303B1 (en) 2013-12-11
CN1373812B (zh) 2012-04-11
ES2214319T3 (es) 2004-09-16
HK1046021A1 (en) 2002-12-20
DE60009323T2 (de) 2005-02-10
KR100527265B1 (ko) 2005-11-09
NO20021223D0 (no) 2002-03-12
BR0014182A (pt) 2002-05-21
AU783873B2 (en) 2005-12-15
WO2001020035A9 (en) 2002-11-07
EP1218542B1 (en) 2004-03-24
CA2384838A1 (en) 2001-03-22
ATE262595T1 (de) 2004-04-15
KR20020047160A (ko) 2002-06-21
MXPA02002656A (es) 2003-10-14
JP2003509068A (ja) 2003-03-11
JP3929775B2 (ja) 2007-06-13
EP1431303A3 (en) 2010-01-06
HK1046021B (en) 2004-10-21
DK1431303T3 (da) 2014-02-17
JP2003116586A (ja) 2003-04-22
EP1431303A2 (en) 2004-06-23
ES2447419T3 (es) 2014-03-12
NO20021223L (no) 2002-05-13
AU7483500A (en) 2001-04-17
DE60009323D1 (de) 2004-04-29

Similar Documents

Publication Publication Date Title
NO20021223L (no) Fremgangsmater og sammensetninger for lineaer, isoterm amplifisering av polynukleotidsekvenser
EP1845160A4 (en) NUCLEOTIDE-amplification
WO2002052031A3 (en) Nucleic acid amplification
EP2272952B8 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
ATE394410T1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
WO2004074503A3 (en) Nucleic acid sequencing methods, kits and reagents
CA2532160A1 (en) Methods of genotyping using differences in melting temperature
DE60123980D1 (de) Amplifizierung von nukleinsäuresequenzen mittels multiplex priming
ATE399880T1 (de) Ein in-vitro-verfahren zur herstellung einer dna- bibliothek
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2005091971A3 (en) Compositions for use in identification of alphaviruses
WO2005044836A3 (de) Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
AU6846698A (en) Method of nucleic acid amplification
WO2001025483A3 (en) Compositions for dna amplification, synthesis, and mutagenesis
ATE475720T1 (de) Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
WO2005054438A3 (en) Nucleic acid molecules containing recombination sites and methods of using the same
WO2003000927A3 (en) Method for the determination of telomere length
DK1815013T3 (da) Sammensætning til at amplificere nucleinsyrer
DK1490383T3 (da) Fremgangsmåde og sammensætning til identifikation og karakterisering af hepatitis C
AR027256A1 (es) Deteccion de microorganismos relacionados con la fermentacion
WO2004085681A3 (en) Global linear non-biased nucleic acid amplification
WO2006081463A3 (en) Thermococcus zilligii dna polymerases and variants thereof
DE50312117D1 (de) Verfahren zur Amplifikation genetischer Information mittels an mehrere Stellen im Genom bindende Primer
WO2001032931A3 (en) Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001051670A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof