DK1407006T3 - Fremgangsmåde til indvinding og oprensning af poxvirus fra inficerede celler - Google Patents

Fremgangsmåde til indvinding og oprensning af poxvirus fra inficerede celler

Info

Publication number
DK1407006T3
DK1407006T3 DK02787945T DK02787945T DK1407006T3 DK 1407006 T3 DK1407006 T3 DK 1407006T3 DK 02787945 T DK02787945 T DK 02787945T DK 02787945 T DK02787945 T DK 02787945T DK 1407006 T3 DK1407006 T3 DK 1407006T3
Authority
DK
Denmark
Prior art keywords
infected cells
recovery
virus
purification
pox virus
Prior art date
Application number
DK02787945T
Other languages
English (en)
Inventor
Karl Heller
Jutta Kramer
Original Assignee
Bavarian Nordic As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic As filed Critical Bavarian Nordic As
Application granted granted Critical
Publication of DK1407006T3 publication Critical patent/DK1407006T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK02787945T 2001-12-20 2002-12-13 Fremgangsmåde til indvinding og oprensning af poxvirus fra inficerede celler DK1407006T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA200101928 2001-12-20

Publications (1)

Publication Number Publication Date
DK1407006T3 true DK1407006T3 (da) 2005-12-12

Family

ID=8160920

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02787945T DK1407006T3 (da) 2001-12-20 2002-12-13 Fremgangsmåde til indvinding og oprensning af poxvirus fra inficerede celler

Country Status (22)

Country Link
US (1) US7056723B2 (da)
EP (1) EP1407006B9 (da)
JP (2) JP5322252B2 (da)
KR (1) KR100947976B1 (da)
CN (1) CN1289663C (da)
AT (1) ATE302268T1 (da)
AU (1) AU2002352247B2 (da)
BR (1) BRPI0215003B1 (da)
CA (1) CA2467099C (da)
DE (1) DE60205631T2 (da)
DK (1) DK1407006T3 (da)
EA (1) EA006485B1 (da)
ES (1) ES2247404T3 (da)
HU (1) HU227507B1 (da)
IL (2) IL161526A0 (da)
MX (1) MXPA04006001A (da)
NO (1) NO335975B1 (da)
NZ (1) NZ533586A (da)
PL (1) PL205929B1 (da)
SI (1) SI1407006T1 (da)
UA (1) UA77735C2 (da)
WO (1) WO2003054175A1 (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
HU230198B1 (hu) * 2000-11-23 2015-10-28 Bavarian Nordic A/S Módosított Vacciniavírus Ankara-variáns
AU2003264144C1 (en) 2002-09-05 2009-12-17 Bavarian Nordic A/S Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
US7951576B2 (en) * 2004-11-05 2011-05-31 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for preparing cells and viruses
US7625570B1 (en) * 2005-03-10 2009-12-01 The Regents Of The University Of California Methods for purifying adeno-associated virus
US8003363B2 (en) 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
AU2008250596C1 (en) * 2007-05-14 2010-11-25 Bavarian Nordic A/S Purification of Vaccinia virus- and recombinant Vaccinia virus-based vaccines
AU2009214768B2 (en) * 2008-02-12 2015-01-22 Sanofi Pasteur Limited Methods using ion exchange and gel filtration chromatography for poxvirus purification
SG176554A1 (en) * 2009-05-12 2012-01-30 Transgene Sa Method for orthopoxvirus production and purification
FR2953686B1 (fr) 2009-12-14 2012-11-02 Agronomique Inst Nat Rech Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
CA2802430C (en) 2010-07-20 2021-07-20 Bavarian Nordic A/S Method for harvesting poxvirus
CN102353792A (zh) * 2011-07-07 2012-02-15 贵州大学 一种基于gpv p32蛋白的间接elisa试剂盒及制备方法
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
EP3624851A1 (en) 2017-05-15 2020-03-25 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
CA3062549A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
EP3847246A1 (en) 2018-09-06 2021-07-14 Bavarian Nordic A/S Storage improved poxvirus compositions
JP7712284B2 (ja) 2020-03-12 2025-07-23 バヴァリアン・ノルディック・アクティーゼルスカブ ポックスウイルスの安定性を改善する組成物
JP2025524459A (ja) 2022-06-29 2025-07-30 バヴァリアン・ノルディック・アクティーゼルスカブ エプスタイン・バー・ウイルスワクチン
IL317745A (en) 2022-06-29 2025-02-01 Bavarian Nordic As Modified recombinant SARNA (VRP) and Vaccinia Ankara virus (MVA) prime regimen
CN115197966A (zh) * 2022-08-15 2022-10-18 深圳源兴基因技术有限公司 一种利用生物反应器大规模生产重组痘苗病毒载体的方法
FR3160186A1 (fr) 2024-03-13 2025-09-19 Odimma Therapeutics Vecteur non-viral pour transcription augmentee
FR3171450A1 (fr) 2025-01-17 2026-07-24 Odimma Therapeutics Virus enveloppé pour la transfection d’acides nucléiques exogènes.

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GB877898A (en) 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
JPS5328989B2 (da) * 1974-05-27 1978-08-17
CH621573A5 (en) 1976-08-05 1981-02-13 Vnii Biosinteza Belkovykh Vesc Process for the continuous disintegration of microorganism cells and system for carrying out this process
JPS54154594A (en) * 1978-05-25 1979-12-05 Sumitomo Chem Co Ltd Extraction of glucose isomerase
WO1991001367A1 (en) * 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
UA68327C2 (en) * 1995-07-04 2004-08-16 Gsf Forschungszentrum Fur Unwe A recombinant mva virus, an isolated eukaryotic cell, infected with recombinant mva virus, a method for production in vitro of polypeptides with use of said cell, a method for production in vitro of virus parts (variants), vaccine containing the recombinant mva virus, a method for immunization of animals
TW426733B (en) 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
ID19548A (id) * 1996-09-24 1998-07-23 Bavarian Nordic Res Inst As Virus mva rekombinan yang mengekspresikan antigen-antigen virus demam dan penggunaan daripadanya dalam vaksin-vaksin
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
NZ299720A (en) * 1996-11-08 1999-07-29 Howick Engineering Ltd Epicyclic input/output annuli of same pcd, with planetary input/output gear sets, and with only one set having aligned teeth
EP0968284B1 (en) * 1996-11-20 2006-12-13 Introgen Therapeutics, Inc. An improved method for the production and purification of adenoviral vectors
US6000551A (en) * 1996-12-20 1999-12-14 Eastman Chemical Company Method for rupturing microalgae cells
FR2787465A1 (fr) * 1998-12-21 2000-06-23 Transgene Sa Procede d'inactivation des virus enveloppes dans une preparation virale de virus non enveloppes
CA2364934C (fr) * 1999-02-22 2011-10-18 Transgene S.A. Procede d'obtention d'une preparation virale purifiee
DE19918619A1 (de) 1999-04-23 2000-10-26 Rhein Biotech Proz & Prod Gmbh Verfahren zum Gewinnen von rekombinantem HBsAg
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen

Also Published As

Publication number Publication date
KR100947976B1 (ko) 2010-03-15
US20040265986A1 (en) 2004-12-30
HUP0402337A2 (hu) 2005-01-28
CN1289663C (zh) 2006-12-13
IL161526A0 (en) 2004-09-27
UA77735C2 (en) 2007-01-15
HK1071765A1 (en) 2005-07-29
AU2002352247B2 (en) 2008-08-07
JP5322252B2 (ja) 2013-10-23
KR20040070263A (ko) 2004-08-06
EP1407006B9 (en) 2006-10-18
EP1407006A1 (en) 2004-04-14
CA2467099A1 (en) 2003-07-03
HU227507B1 (hu) 2011-07-28
HUP0402337A3 (en) 2010-01-28
CN1606616A (zh) 2005-04-13
DE60205631T2 (de) 2006-06-08
US7056723B2 (en) 2006-06-06
WO2003054175A1 (en) 2003-07-03
NO20043181L (no) 2004-07-19
PL369498A1 (en) 2005-04-18
NZ533586A (en) 2005-02-25
EP1407006B1 (en) 2005-08-17
EA200400833A1 (ru) 2004-12-30
JP2005512565A (ja) 2005-05-12
CA2467099C (en) 2013-01-29
SI1407006T1 (sl) 2006-04-30
EA006485B1 (ru) 2005-12-29
BR0215003A (pt) 2004-11-09
NO335975B1 (no) 2015-04-07
MXPA04006001A (es) 2005-08-19
IL161526A (en) 2009-11-18
JP2010046093A (ja) 2010-03-04
DE60205631D1 (de) 2005-09-22
AU2002352247A1 (en) 2003-07-09
BRPI0215003B1 (pt) 2017-03-14
ES2247404T3 (es) 2006-03-01
PL205929B1 (pl) 2010-06-30
ATE302268T1 (de) 2005-09-15

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