ATE302268T1 - Methode zur gewinnung und reinigung von poxviren aus infizierten zellen - Google Patents

Methode zur gewinnung und reinigung von poxviren aus infizierten zellen

Info

Publication number
ATE302268T1
ATE302268T1 AT02787945T AT02787945T ATE302268T1 AT E302268 T1 ATE302268 T1 AT E302268T1 AT 02787945 T AT02787945 T AT 02787945T AT 02787945 T AT02787945 T AT 02787945T AT E302268 T1 ATE302268 T1 AT E302268T1
Authority
AT
Austria
Prior art keywords
infected cells
obtaining
virus
present
virus containing
Prior art date
Application number
AT02787945T
Other languages
English (en)
Inventor
Karl Heller
Jutta Kramer
Original Assignee
Bavarian Nordic As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic As filed Critical Bavarian Nordic As
Application granted granted Critical
Publication of ATE302268T1 publication Critical patent/ATE302268T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Medicines Containing Plant Substances (AREA)
AT02787945T 2001-12-20 2002-12-13 Methode zur gewinnung und reinigung von poxviren aus infizierten zellen ATE302268T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101928 2001-12-20
PCT/EP2002/014179 WO2003054175A1 (en) 2001-12-20 2002-12-13 Method for the recovery and purification of poxviruses from infected cells

Publications (1)

Publication Number Publication Date
ATE302268T1 true ATE302268T1 (de) 2005-09-15

Family

ID=8160920

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02787945T ATE302268T1 (de) 2001-12-20 2002-12-13 Methode zur gewinnung und reinigung von poxviren aus infizierten zellen

Country Status (22)

Country Link
US (1) US7056723B2 (de)
EP (1) EP1407006B9 (de)
JP (2) JP5322252B2 (de)
KR (1) KR100947976B1 (de)
CN (1) CN1289663C (de)
AT (1) ATE302268T1 (de)
AU (1) AU2002352247B2 (de)
BR (1) BRPI0215003B1 (de)
CA (1) CA2467099C (de)
DE (1) DE60205631T2 (de)
DK (1) DK1407006T3 (de)
EA (1) EA006485B1 (de)
ES (1) ES2247404T3 (de)
HU (1) HU227507B1 (de)
IL (2) IL161526A0 (de)
MX (1) MXPA04006001A (de)
NO (1) NO335975B1 (de)
NZ (1) NZ533586A (de)
PL (1) PL205929B1 (de)
SI (1) SI1407006T1 (de)
UA (1) UA77735C2 (de)
WO (1) WO2003054175A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
WO2002042480A2 (en) 2000-11-23 2002-05-30 Bavarian Nordic A/S Modified vaccinia ankara virus variant
SI1434858T2 (sl) * 2002-09-05 2019-05-31 Bavarian Nordic A/S Postopek za pomnoževanje poksvirusa v razmerah brez seruma
US7951576B2 (en) * 2004-11-05 2011-05-31 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for preparing cells and viruses
US7625570B1 (en) * 2005-03-10 2009-12-01 The Regents Of The University Of California Methods for purifying adeno-associated virus
AU2008250596C1 (en) * 2007-05-14 2010-11-25 Bavarian Nordic A/S Purification of Vaccinia virus- and recombinant Vaccinia virus-based vaccines
US8003363B2 (en) 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
BRPI0908474A2 (pt) * 2008-02-12 2016-07-26 Sanofi Pasteur Ltd métodos de uso de cromatografia de troca de íon e de filtração de gel para purificação de vírus causador da catapora
SG176554A1 (en) * 2009-05-12 2012-01-30 Transgene Sa Method for orthopoxvirus production and purification
FR2953686B1 (fr) * 2009-12-14 2012-11-02 Agronomique Inst Nat Rech Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
US10087423B2 (en) 2010-07-20 2018-10-02 Bavarian Nordic A/S Method for harvesting expression products
CN102353792A (zh) * 2011-07-07 2012-02-15 贵州大学 一种基于gpv p32蛋白的间接elisa试剂盒及制备方法
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
KR20200003921A (ko) 2017-05-15 2020-01-10 얀센 백신스 앤드 프리벤션 비.브이. 안정한 바이러스-함유 조성물
WO2018211419A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
EP3847246A1 (de) 2018-09-06 2021-07-14 Bavarian Nordic A/S Pockenviruszusammensetzungen mit verbesserter lagerung
EP4117722A1 (de) 2020-03-12 2023-01-18 Bavarian Nordic A/S Zusammensetzungen zur verbesserung der pockenvirusstabilität
EP4547273A1 (de) 2022-06-29 2025-05-07 Bavarian Nordic A/S Prime-boost-therapie mit rekombinantem modifiziertem sarna (vrp) und vacciniavirus ankara (mva)
CN119604618A (zh) 2022-06-29 2025-03-11 巴法里安诺迪克有限公司 爱泼斯坦-巴尔病毒疫苗
CN115197966A (zh) * 2022-08-15 2022-10-18 深圳源兴基因技术有限公司 一种利用生物反应器大规模生产重组痘苗病毒载体的方法
FR3160186A1 (fr) 2024-03-13 2025-09-19 Odimma Therapeutics Vecteur non-viral pour transcription augmentee

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GB877898A (en) 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
JPS5328989B2 (de) 1974-05-27 1978-08-17
CH621573A5 (en) 1976-08-05 1981-02-13 Vnii Biosinteza Belkovykh Vesc Process for the continuous disintegration of microorganism cells and system for carrying out this process
JPS54154594A (en) 1978-05-25 1979-12-05 Sumitomo Chem Co Ltd Extraction of glucose isomerase
WO1991001367A1 (en) 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
UA68327C2 (en) * 1995-07-04 2004-08-16 Gsf Forschungszentrum Fur Unwe A recombinant mva virus, an isolated eukaryotic cell, infected with recombinant mva virus, a method for production in vitro of polypeptides with use of said cell, a method for production in vitro of virus parts (variants), vaccine containing the recombinant mva virus, a method for immunization of animals
TW426733B (en) 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
WO1998013500A2 (en) * 1996-09-24 1998-04-02 Bavarian Nordic Research Institute A/S Recombinant mva virus expressing dengue virus antigens, and the use thereof in vaccines
US5721120A (en) 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
NZ299720A (en) * 1996-11-08 1999-07-29 Howick Engineering Ltd Epicyclic input/output annuli of same pcd, with planetary input/output gear sets, and with only one set having aligned teeth
BR9713368A (pt) * 1996-11-20 2001-09-18 Introgen Therapeutics Inc Processo melhorado para a produção e a purificação de vetores adenovirais
US6000551A (en) 1996-12-20 1999-12-14 Eastman Chemical Company Method for rupturing microalgae cells
FR2787465A1 (fr) * 1998-12-21 2000-06-23 Transgene Sa Procede d'inactivation des virus enveloppes dans une preparation virale de virus non enveloppes
WO2000050573A1 (fr) * 1999-02-22 2000-08-31 Transgene S.A. Procede d'obtention d'une preparation virale purifiee
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US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen

Also Published As

Publication number Publication date
PL369498A1 (en) 2005-04-18
EA006485B1 (ru) 2005-12-29
DE60205631T2 (de) 2006-06-08
EP1407006B1 (de) 2005-08-17
AU2002352247B2 (en) 2008-08-07
IL161526A (en) 2009-11-18
HUP0402337A3 (en) 2010-01-28
KR20040070263A (ko) 2004-08-06
US7056723B2 (en) 2006-06-06
AU2002352247A1 (en) 2003-07-09
DE60205631D1 (de) 2005-09-22
NZ533586A (en) 2005-02-25
NO20043181L (no) 2004-07-19
JP5322252B2 (ja) 2013-10-23
CN1606616A (zh) 2005-04-13
EP1407006B9 (de) 2006-10-18
CA2467099C (en) 2013-01-29
ES2247404T3 (es) 2006-03-01
CN1289663C (zh) 2006-12-13
SI1407006T1 (sl) 2006-04-30
UA77735C2 (en) 2007-01-15
IL161526A0 (en) 2004-09-27
CA2467099A1 (en) 2003-07-03
WO2003054175A1 (en) 2003-07-03
KR100947976B1 (ko) 2010-03-15
US20040265986A1 (en) 2004-12-30
HU227507B1 (hu) 2011-07-28
HUP0402337A2 (hu) 2005-01-28
JP2010046093A (ja) 2010-03-04
BR0215003A (pt) 2004-11-09
BRPI0215003B1 (pt) 2017-03-14
MXPA04006001A (es) 2005-08-19
DK1407006T3 (da) 2005-12-12
NO335975B1 (no) 2015-04-07
PL205929B1 (pl) 2010-06-30
EA200400833A1 (ru) 2004-12-30
HK1071765A1 (en) 2005-07-29
EP1407006A1 (de) 2004-04-14
JP2005512565A (ja) 2005-05-12

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