DK1470229T3 - Proteinmærke, der omfatter et biotinyleringsdomæne, og fremgangsmåde til at foröge oplöselighed og til at bestemme foldningstilstand - Google Patents

Proteinmærke, der omfatter et biotinyleringsdomæne, og fremgangsmåde til at foröge oplöselighed og til at bestemme foldningstilstand

Info

Publication number
DK1470229T3
DK1470229T3 DK03734757T DK03734757T DK1470229T3 DK 1470229 T3 DK1470229 T3 DK 1470229T3 DK 03734757 T DK03734757 T DK 03734757T DK 03734757 T DK03734757 T DK 03734757T DK 1470229 T3 DK1470229 T3 DK 1470229T3
Authority
DK
Denmark
Prior art keywords
protein
biotinylation domain
folding state
protein tag
increasing solubility
Prior art date
Application number
DK03734757T
Other languages
English (en)
Inventor
Mitali Samaddar
Jonathan Michael Blackburn
Darren James Hart
Michael R Dyson
Original Assignee
Sense Proteomic Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sense Proteomic Ltd filed Critical Sense Proteomic Ltd
Application granted granted Critical
Publication of DK1470229T3 publication Critical patent/DK1470229T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Burglar Alarm Systems (AREA)
  • Radar Systems Or Details Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03734757T 2002-01-29 2003-01-29 Proteinmærke, der omfatter et biotinyleringsdomæne, og fremgangsmåde til at foröge oplöselighed og til at bestemme foldningstilstand DK1470229T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0202018.8A GB0202018D0 (en) 2002-01-29 2002-01-29 Tag and method

Publications (1)

Publication Number Publication Date
DK1470229T3 true DK1470229T3 (da) 2006-10-02

Family

ID=9929956

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03734757T DK1470229T3 (da) 2002-01-29 2003-01-29 Proteinmærke, der omfatter et biotinyleringsdomæne, og fremgangsmåde til at foröge oplöselighed og til at bestemme foldningstilstand

Country Status (11)

Country Link
US (1) US8999897B2 (da)
EP (1) EP1470229B1 (da)
JP (1) JP4377242B2 (da)
AT (1) ATE328092T1 (da)
AU (1) AU2003238441B2 (da)
CA (1) CA2474457C (da)
DE (1) DE60305643T2 (da)
DK (1) DK1470229T3 (da)
ES (1) ES2268376T3 (da)
GB (1) GB0202018D0 (da)
WO (1) WO2003064656A1 (da)

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US7094568B2 (en) * 2000-08-17 2006-08-22 Sense Proteomic Ltd. Method for producing proteins tagged at the N- or C-terminus
US20040002078A1 (en) * 2001-12-05 2004-01-01 Sense Proteomic Limited Arrays
GB0202018D0 (en) 2002-01-29 2002-03-13 Sense Proteomic Ltd Tag and method
GB0205910D0 (en) * 2002-03-13 2002-04-24 Sense Proteomic Ltd Arrays and methods
US20030228709A1 (en) * 2002-03-25 2003-12-11 Kozlowski Roland Zbignieiw Arrays
GB0419628D0 (en) * 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
GB2442048B (en) * 2006-07-25 2009-09-30 Proimmune Ltd Biotinylated MHC complexes and their uses
EP2137308B1 (en) 2007-03-26 2016-08-03 Agenus Inc. Cell surface display, screening and production of proteins of interest
GB2460717A (en) 2008-06-11 2009-12-16 Sense Proteomic Ltd Autoantibodies for the detection of a predisposition to Lupus
GB2478734A (en) 2010-03-15 2011-09-21 Sense Proteomic Ltd Auto-antibody biomarkers of prostate cancer
GB201017520D0 (en) 2010-10-15 2010-12-01 Sense Proteomic Ltd Biomarkers
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
CN105636976A (zh) 2012-07-20 2016-06-01 庆熙大学校产学协力团 新型肽标签以及其用途
US9758571B2 (en) 2012-07-20 2017-09-12 University—Industry Cooperation Group Of Kyung Hee University Antibody for epitope tagging, hybridoma cell line and uses thereof
EP3443001B1 (en) 2016-04-11 2025-04-30 Obsidian Therapeutics, Inc. REGULATED BIOCIRCUIT SYSTEMS
JP7660343B2 (ja) 2017-03-03 2025-04-11 オブシディアン セラピューティクス， インコーポレイテッド 免疫療法のための組成物及び方法
KR102746901B1 (ko) 2017-03-03 2024-12-26 옵시디안 테라퓨틱스, 인크. Cd19 조성물 및 면역요법을 위한 방법
EP3806888B1 (en) 2018-06-12 2024-01-31 Obsidian Therapeutics, Inc. Pde5 derived regulatory constructs and methods of use in immunotherapy
US20210386788A1 (en) 2018-10-24 2021-12-16 Obsidian Therapeutics, Inc. Er tunable protein regulation
SG10201811119XA (en) * 2018-12-12 2020-07-29 Sengenics Sdn Bhd Detection of biomarkers for non-small cell lung cancer
SG11202109172TA (en) 2019-03-08 2021-09-29 Obsidian Therapeutics Inc Human carbonic anhydrase 2 compositions and methods for tunable regulation
EP3983537A1 (en) 2019-06-12 2022-04-20 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
JP2022537670A (ja) 2019-06-12 2022-08-29 オブシディアン セラピューティクス， インコーポレイテッド Ｃａ２の組成物および調整可能な制御方法
US20220348937A1 (en) 2019-09-06 2022-11-03 Obsidian Therapeutics, Inc. Compositions and methods for dhfr tunable protein regulation
SG10201908922UA (en) * 2019-09-25 2021-04-29 Sengenics Corp Pte Ltd Identification of health status in the elderly using immunological biomarkers
CN117716236A (zh) * 2020-11-18 2024-03-15 盛捷宁克斯私人有限公司 用于预测类风湿性关节炎患者对阿达莫单抗的免疫原性和治疗反应的生物标志物

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CA2057908A1 (en) 1989-05-19 1990-11-20 John E. Cronan, Jr. Fusion proteins having an in vivo post-translational modification site and methods of manufacture and purification
US5252466A (en) * 1989-05-19 1993-10-12 Biotechnology Research And Development Corporation Fusion proteins having a site for in vivo post-translation modification and methods of making and purifying them
US6072039A (en) 1991-04-19 2000-06-06 Rohm And Haas Company Hybrid polypeptide comparing a biotinylated avidin binding polypeptide fused to a polypeptide of interest
US5801233A (en) * 1992-10-02 1998-09-01 Arch Development Corporation Nucleic acid compositions encoding acetyl-coa carboxylase and uses therefor
AU7516694A (en) * 1993-07-30 1995-02-28 Affymax Technologies N.V. Biotinylation of proteins
FR2717499B1 (fr) * 1994-03-17 1996-05-24 Ulp Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.
JP3466765B2 (ja) * 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
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US7816098B2 (en) * 2000-01-31 2010-10-19 Sense Proteomic Limited Methods of making and using a protein array
ATE477489T1 (de) 2000-01-31 2010-08-15 Sense Proteomic Ltd Verfahren zur herstellung von protein expression arrays und anwendung derselben in schnellem screening
US7148058B2 (en) * 2000-06-05 2006-12-12 Chiron Corporation Protein microarrays on mirrored surfaces for performing proteomic analyses
GB2384779B (en) 2000-08-17 2004-09-29 Sense Proteomic Ltd Method for the production of tagged proteins
US7094568B2 (en) * 2000-08-17 2006-08-22 Sense Proteomic Ltd. Method for producing proteins tagged at the N- or C-terminus
CA2433057A1 (en) 2000-12-26 2002-08-22 Applied Molecular Evolution, Inc. Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use
ES2349032T3 (es) 2001-06-01 2010-12-22 Pgxhealth, Llc Polimorfismos en el gen humano del polipéptido 2c8 citocromo p450 y su uso en aplicaciones diagnósticas y terapéuticas.
US20040002078A1 (en) * 2001-12-05 2004-01-01 Sense Proteomic Limited Arrays
GB0202018D0 (en) 2002-01-29 2002-03-13 Sense Proteomic Ltd Tag and method
GB0205910D0 (en) * 2002-03-13 2002-04-24 Sense Proteomic Ltd Arrays and methods
US20030228709A1 (en) * 2002-03-25 2003-12-11 Kozlowski Roland Zbignieiw Arrays

Also Published As

Publication number Publication date
ES2268376T3 (es) 2007-03-16
DE60305643D1 (de) 2006-07-06
CA2474457C (en) 2014-04-15
GB0202018D0 (en) 2002-03-13
WO2003064656A1 (en) 2003-08-07
JP2005516074A (ja) 2005-06-02
EP1470229B1 (en) 2006-05-31
US20050221308A1 (en) 2005-10-06
AU2003238441B2 (en) 2008-10-30
DE60305643T2 (de) 2007-05-03
US8999897B2 (en) 2015-04-07
ATE328092T1 (de) 2006-06-15
CA2474457A1 (en) 2003-08-07
JP4377242B2 (ja) 2009-12-02
EP1470229A1 (en) 2004-10-27

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