DK1483576T3 - Hidtil ukendt fremgangsmåde til detektion og analysering af proteininteraktioner in vivo - Google Patents
Hidtil ukendt fremgangsmåde til detektion og analysering af proteininteraktioner in vivoInfo
- Publication number
- DK1483576T3 DK1483576T3 DK03743880.1T DK03743880T DK1483576T3 DK 1483576 T3 DK1483576 T3 DK 1483576T3 DK 03743880 T DK03743880 T DK 03743880T DK 1483576 T3 DK1483576 T3 DK 1483576T3
- Authority
- DK
- Denmark
- Prior art keywords
- protein
- cell
- interaction
- protein interactions
- detection
- Prior art date
Links
- 230000006916 protein interaction Effects 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 5
- 238000001727 in vivo Methods 0.000 title 1
- 238000001514 detection method Methods 0.000 abstract 4
- 230000003993 interaction Effects 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102000018120 Recombinases Human genes 0.000 abstract 1
- 108010091086 Recombinases Proteins 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10211063A DE10211063A1 (de) | 2002-03-13 | 2002-03-13 | Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo |
| PCT/EP2003/002611 WO2003076932A2 (de) | 2002-03-13 | 2003-03-13 | Detektion und analyse von protein-interaktionen in vivo |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1483576T3 true DK1483576T3 (da) | 2011-01-31 |
Family
ID=27797747
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09165808.8T DK2110666T3 (da) | 2002-03-13 | 2003-03-13 | Hidtil ukendt fremgangsmåde til at detektere og analysere proteininteraktioner in vivo |
| DK03743880.1T DK1483576T3 (da) | 2002-03-13 | 2003-03-13 | Hidtil ukendt fremgangsmåde til detektion og analysering af proteininteraktioner in vivo |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09165808.8T DK2110666T3 (da) | 2002-03-13 | 2003-03-13 | Hidtil ukendt fremgangsmåde til at detektere og analysere proteininteraktioner in vivo |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7585635B2 (da) |
| EP (2) | EP2110666B1 (da) |
| AT (1) | ATE483971T1 (da) |
| AU (1) | AU2003215660A1 (da) |
| DE (2) | DE10211063A1 (da) |
| DK (2) | DK2110666T3 (da) |
| WO (1) | WO2003076932A2 (da) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7695723B2 (en) | 2002-12-31 | 2010-04-13 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| US7785601B2 (en) | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| US20070224615A1 (en) * | 2003-07-09 | 2007-09-27 | Invitrogen Corporation | Methods for assaying protein-protein interactions |
| US7049076B2 (en) | 2003-07-09 | 2006-05-23 | Sentigen Biosciences, Inc. | Method for assaying protein—protein interaction |
| DE10343375A1 (de) * | 2003-09-17 | 2005-04-28 | Axaron Bioscience Ag | Verfahren zur Detektion und Analyse von Protein-Protein-Interaktionen |
| AU2005258000B2 (en) * | 2004-06-21 | 2011-02-03 | Progenra Inc. | Diagnostic and screening methods and kits associated with proteolytic activity |
| EP1893627A4 (en) * | 2005-05-27 | 2009-11-11 | Sentigen Biosciences Inc | MULTIPLEXARRAY SUITABLE FOR THE INVESTIGATION OF PROTEIN-PROTEIN INTERACTIONS |
| US20070026012A1 (en) * | 2005-08-01 | 2007-02-01 | Cornell Research Foundation, Inc. | Compositions and methods for monitoring and altering protein folding and solubility |
| CA2620934C (en) * | 2005-08-22 | 2014-07-22 | Cornell Research Foundation, Inc. | Compositions and methods for determining protein interactions using tat signal sequence and marker protein |
| WO2007030937A2 (en) * | 2005-09-16 | 2007-03-22 | Institut De Recherches Cliniques De Montreal/I.R.C.M. | Screening proteinase modulators using a chimeric protein and ski-i proprotein convertase substrates and inhibitors |
| JP5346583B2 (ja) * | 2005-09-28 | 2013-11-20 | アッタゲネ インコーポレーテッド | 生物検体の生物活性を解析するための方法及び構築物、並びに生物の状態を測定するための方法及び構築物 |
| EP2002021B1 (en) * | 2006-03-16 | 2011-10-12 | Life Technologies Corporation | Methods for assaying protein-protein interaction |
| WO2008089132A2 (en) * | 2007-01-12 | 2008-07-24 | Cornell Research Foundation, Inc. | Genetic selection for protein folding and solubility in the bacterial periplasm |
| EP2344676A1 (en) * | 2008-09-25 | 2011-07-20 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Combinatorial synthesis and use of libraries of short expressed nucleic acid sequences for the analysis of cellular events |
| US9169312B2 (en) * | 2010-09-21 | 2015-10-27 | San Diego State University Research Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| WO2013022739A1 (en) * | 2011-08-05 | 2013-02-14 | Leonard Joshua N | Modular extracellular sensor architecture for cell-based biosensors |
| CN107207371A (zh) * | 2014-10-20 | 2017-09-26 | 斯克利普斯研究院 | 用于选择结合配偶体的基于邻近度的方法 |
| SG10201910999TA (en) * | 2015-05-15 | 2020-01-30 | Agency Science Tech & Res | Native protein purification technology |
| MX2018008345A (es) | 2016-01-11 | 2018-12-06 | Univ Leland Stanford Junior | Proteínas quiméricas y métodos de inmunoterapia. |
| IL304088A (en) | 2016-01-11 | 2023-08-01 | Univ Leland Stanford Junior | Systems containing chimeric proteins and their uses for controlling gene expression |
| WO2018029682A1 (en) | 2016-08-08 | 2018-02-15 | Ramot At Tel-Aviv University Ltd. | Bacterial systems for analyzing ubiquitylated polypeptides |
| WO2018175856A1 (en) * | 2017-03-23 | 2018-09-27 | Northwestern University | Integration of mesa receptors and promoters to implement customized cellular function |
| EP3634992B1 (en) * | 2017-06-08 | 2024-03-27 | The Brigham And Women's Hospital, Inc. | Methods and compositions for identifying epitopes |
| US11932846B2 (en) | 2017-08-08 | 2024-03-19 | Technology Innovation Momentum Fund (Israel) Limited Partnership | Chloramphenicol resistant split protein and uses thereof |
| US11649509B2 (en) | 2017-11-16 | 2023-05-16 | The Johns Hopkins University | Anastasis biosensor caspase tracker |
| SG11202009787VA (en) * | 2018-04-26 | 2020-11-27 | Paean Biotechnology Inc | Modified mitochondria and use thereof |
| EP3831946A4 (en) * | 2018-07-31 | 2022-08-10 | The University of Tokyo | METHOD OF MEASUREMENT OF MEMBRANE PROTEIN ACTIVITY |
| EP3891279A1 (en) * | 2018-12-07 | 2021-10-13 | Octant, Inc. | Systems for protein-protein interaction screening |
| WO2021178965A1 (en) * | 2020-03-06 | 2021-09-10 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Novel split-luciferase enzymes and applications thereof |
| WO2022094463A1 (en) * | 2020-11-02 | 2022-05-05 | Regents Of The University Of Minnesota | Live cell assay for protease inhibition |
| WO2022183024A2 (en) * | 2021-02-26 | 2022-09-01 | Cornell University | Cell-based assay for protease inhibitors |
| SE547227C2 (en) * | 2023-10-27 | 2025-06-10 | Markus Schmid | Identification of (poly)peptide-(poly)peptide interactions |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1293460C (en) | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| EP0626999A4 (en) * | 1992-01-24 | 1997-04-09 | Life Technologies Inc | Modulation of enzyme activities in the -i(in vivo) cloning of dna. |
| US5935580A (en) * | 1992-04-21 | 1999-08-10 | Institut Pasteur | Recombinant mutants for inducing specific immune responses |
| EP0637335B1 (en) * | 1992-04-21 | 2007-07-25 | Institut Pasteur | Recombinant mutants for inducing specific immune responses |
| US5585245A (en) | 1994-04-22 | 1996-12-17 | California Institute Of Technology | Ubiquitin-based split protein sensor |
| US5695941A (en) * | 1994-06-22 | 1997-12-09 | The General Hospital Corporation | Interaction trap systems for analysis of protein networks |
| FR2734567B1 (fr) * | 1995-05-26 | 1997-08-08 | Commissariat Energie Atomique | Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines |
| DE19650714A1 (de) * | 1996-12-06 | 1998-06-10 | Melchner Harald Von Prof Dr | Genfallen-Konstrukt zur Identifizierung und Isolierung von Genen |
| AU721549B2 (en) * | 1996-12-11 | 2000-07-06 | Bristol-Myers Squibb Company | Prokaryotic two-hybrid system |
| US6294330B1 (en) | 1997-01-31 | 2001-09-25 | Odyssey Pharmaceuticals Inc. | Protein fragment complementation assays for the detection of biological or drug interactions |
| GB9718591D0 (en) | 1997-09-03 | 1997-11-05 | Zeneca Ltd | Methods |
| US5885779A (en) * | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
| US6828099B2 (en) | 1998-02-02 | 2004-12-07 | Odyssey Thera Inc. | Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase |
| US6303627B1 (en) * | 1998-06-19 | 2001-10-16 | Eli Lilly And Company | Inhibitors of serotonin reuptake |
| WO2001066787A1 (en) * | 2000-03-08 | 2001-09-13 | Tvw Telethon Institute For Child Health Research | Improved reverse n-hybrid screening method |
| AUPQ776100A0 (en) * | 2000-05-26 | 2000-06-15 | Australian National University, The | Synthetic molecules and uses therefor |
-
2002
- 2002-03-13 DE DE10211063A patent/DE10211063A1/de not_active Withdrawn
-
2003
- 2003-03-13 DK DK09165808.8T patent/DK2110666T3/da active
- 2003-03-13 EP EP09165808A patent/EP2110666B1/de not_active Expired - Lifetime
- 2003-03-13 US US10/507,506 patent/US7585635B2/en not_active Expired - Fee Related
- 2003-03-13 AT AT03743880T patent/ATE483971T1/de active
- 2003-03-13 DE DE50313154T patent/DE50313154D1/de not_active Expired - Lifetime
- 2003-03-13 DK DK03743880.1T patent/DK1483576T3/da active
- 2003-03-13 WO PCT/EP2003/002611 patent/WO2003076932A2/de not_active Ceased
- 2003-03-13 AU AU2003215660A patent/AU2003215660A1/en not_active Abandoned
- 2003-03-13 EP EP03743880A patent/EP1483576B1/de not_active Expired - Lifetime
-
2009
- 2009-05-01 US US12/453,216 patent/US8349619B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE50313154D1 (de) | 2010-11-18 |
| US20050084864A1 (en) | 2005-04-21 |
| EP2110666A1 (de) | 2009-10-21 |
| WO2003076932A3 (de) | 2004-09-02 |
| AU2003215660A1 (en) | 2003-09-22 |
| US8349619B2 (en) | 2013-01-08 |
| EP1483576A2 (de) | 2004-12-08 |
| WO2003076932A2 (de) | 2003-09-18 |
| EP2110666B1 (de) | 2012-12-26 |
| ATE483971T1 (de) | 2010-10-15 |
| DE10211063A1 (de) | 2003-10-09 |
| US20090298089A1 (en) | 2009-12-03 |
| AU2003215660A8 (en) | 2003-09-22 |
| EP1483576B1 (de) | 2010-10-06 |
| US7585635B2 (en) | 2009-09-08 |
| DK2110666T3 (da) | 2013-04-08 |
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