DK1499348T3 - Fremgangsmåde til fremstilling af infektiøse influenza b-vira i cellekultur - Google Patents

Fremgangsmåde til fremstilling af infektiøse influenza b-vira i cellekultur Download PDF

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DK1499348T3
DK1499348T3 DK03724208.8T DK03724208T DK1499348T3 DK 1499348 T3 DK1499348 T3 DK 1499348T3 DK 03724208 T DK03724208 T DK 03724208T DK 1499348 T3 DK1499348 T3 DK 1499348T3
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influenza
virus
cells
viruses
mdv
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DK03724208.8T
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Erich Hoffman
Greg Duke
George William Kemble
Hong Jin
Bin Lu
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Medimmune Llc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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    • C12N7/02—Recovery or purification
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16141—Use of virus, viral particle or viral elements as a vector
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16161—Methods of inactivation or attenuation
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16241—Use of virus, viral particle or viral elements as a vector
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16261—Methods of inactivation or attenuation
    • C12N2760/16262—Methods of inactivation or attenuation by genetic engineering

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Claims (9)

1. Fremgangsmåde til fremstilling af infektiøse influenza B-vira i cellekultur, hvilken fremgangsmåde omfatter: i) elektroporering af en population af Vero-celler med et antal plasmidvektorer, der omfatter nukleinsyresekvenser af et influenza B-virusgenom; ii) dyrkning af populationen af Vero-celler under betingelser, der åbner mulighed for viral replikation; og iii) indvinding af et antal infektiøse influenza B-vira.
2. Fremgangsmåde ifølge krav 1, hvor influenza B-viraene har et eller flere fænotypiske træk, der er udvalgt fra gruppen, der består af: temperaturfølsomhed, kuldetilpasning og svækkelse.
3. Fremgangsmåde ifølge krav 1, hvor influenza B-viraene omfatter et svækket, kuldetilpasset, temperaturfølsomt influenzavirus.
4. Fremgangsmåde ifølge krav 1, der omfatter dyrkning af Vero-cellerne i serumfrit medium.
5. Fremgangsmåde ifølge krav 1, hvor betingelserne, der åbner mulighed for viral replikation, omfatter dyrkning af Vero-cellerne ved en temperatur under eller på 35 °C.
6. Fremgangsmåde ifølge krav 1, hvor betingelserne, der åbner mulighed for viral replikation, omfatter dyrkning af Vero-cellerne ved en temperatur på 33 °C.
7. Fremgangsmåde ifølge krav 1, hvor influenza B-virusset er egnet til administration i en intranasal vaccineformulering.
8. Fremgangsmåde ifølge krav 1, hvor antallet af plasmidvektorer er otte plasmidvektorer.
9. Fremgangsmåde ifølge krav 1, hvor der ikke er noget hjælpevirus .
DK03724208.8T 2002-04-26 2003-04-25 Fremgangsmåde til fremstilling af infektiøse influenza b-vira i cellekultur DK1499348T3 (da)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US37567502P 2002-04-26 2002-04-26
US39498302P 2002-07-09 2002-07-09
US41057602P 2002-09-12 2002-09-12
US41980202P 2002-10-18 2002-10-18
US42070802P 2002-10-23 2002-10-23
US45769903P 2003-03-24 2003-03-24
US46236103P 2003-04-10 2003-04-10
PCT/US2003/012728 WO2003091401A2 (en) 2002-04-26 2003-04-25 Multi plasmid system for the production of influenza virus

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DK1499348T3 true DK1499348T3 (da) 2015-01-05

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US (3) US8012736B2 (da)
EP (2) EP1499348B1 (da)
JP (4) JP2005523698A (da)
KR (2) KR101229202B1 (da)
CN (4) CN103540613A (da)
AR (2) AR039465A1 (da)
AU (2) AU2003231083A1 (da)
CA (1) CA2482946C (da)
DK (1) DK1499348T3 (da)
ES (1) ES2526191T3 (da)
TW (2) TWI347363B (da)
WO (1) WO2003091401A2 (da)

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