DK1546328T3 - Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster - Google Patents

Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster

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Publication number
DK1546328T3
DK1546328T3 DK03776861T DK03776861T DK1546328T3 DK 1546328 T3 DK1546328 T3 DK 1546328T3 DK 03776861 T DK03776861 T DK 03776861T DK 03776861 T DK03776861 T DK 03776861T DK 1546328 T3 DK1546328 T3 DK 1546328T3
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DK
Denmark
Prior art keywords
cell
gene product
starting
high yield
dna sequence
Prior art date
Application number
DK03776861T
Other languages
English (en)
Inventor
Volker Sandig
Karsten Winkler
Uwe Marx
Tobias Wermelinger
Original Assignee
Probiogen Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Probiogen Ag filed Critical Probiogen Ag
Application granted granted Critical
Publication of DK1546328T3 publication Critical patent/DK1546328T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK03776861T 2002-10-04 2003-10-06 Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster DK1546328T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02022194A EP1405908A1 (en) 2002-10-04 2002-10-04 Creation of high yield heterologous expression cell lines

Publications (1)

Publication Number Publication Date
DK1546328T3 true DK1546328T3 (da) 2006-08-07

Family

ID=31985049

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03776861T DK1546328T3 (da) 2002-10-04 2003-10-06 Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster

Country Status (9)

Country Link
US (1) US20060148085A1 (da)
EP (2) EP1405908A1 (da)
JP (1) JP2006500937A (da)
AT (1) ATE324439T1 (da)
AU (1) AU2003286135B2 (da)
CA (1) CA2501218A1 (da)
DE (1) DE60304881T2 (da)
DK (1) DK1546328T3 (da)
WO (1) WO2004031383A1 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104109712A (zh) 2004-02-18 2014-10-22 克罗莫塞尔公司 使用信号探针的方法和材料
CN101061214B (zh) * 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合
CA2716801A1 (en) * 2008-03-12 2009-09-17 Wyeth Llc Methods for identifying cells suitable for large-scale production of recombinant proteins
EP2105505A1 (en) 2008-03-28 2009-09-30 Celonic AG Methods and materials for the reproducible generation of high producer cell lines for recombinant proteins
US20110136236A1 (en) * 2008-04-21 2011-06-09 Unitargeting Research As Genetically modified eukaryotic cells
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
GB201703417D0 (en) 2017-03-03 2017-04-19 Ge Healthcare Bio Sciences Ab Method for cell line development
WO2023039463A1 (en) * 2021-09-09 2023-03-16 Bioconsortia, Inc. Blind editing of polynucleotide sequences
WO2024137877A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6086913A (en) * 1995-11-01 2000-07-11 University Of British Columbia Liposomal delivery of AAV vectors
US6534314B1 (en) * 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
US6262341B1 (en) * 1997-11-18 2001-07-17 Pioneer Hi-Bred International, Inc. Method for the integration of foreign DNA into eukaryotic genomes
CN1455817A (zh) * 2000-07-21 2003-11-12 (由农业部部长代表的)美利坚合众国 用于在真核基因组中置换、转移和富积dna的方法

Also Published As

Publication number Publication date
ATE324439T1 (de) 2006-05-15
WO2004031383A1 (en) 2004-04-15
AU2003286135A1 (en) 2004-04-23
AU2003286135B2 (en) 2009-01-15
CA2501218A1 (en) 2004-04-15
EP1546328B1 (en) 2006-04-26
EP1405908A1 (en) 2004-04-07
JP2006500937A (ja) 2006-01-12
US20060148085A1 (en) 2006-07-06
EP1546328A1 (en) 2005-06-29
DE60304881D1 (de) 2006-06-01
DE60304881T2 (de) 2007-04-19

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