DK1556512T3 - Bgl6 beta-glucosidase og nukleinsyrer, der koder herfor - Google Patents
Bgl6 beta-glucosidase og nukleinsyrer, der koder herfor Download PDFInfo
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- DK1556512T3 DK1556512T3 DK03768790.2T DK03768790T DK1556512T3 DK 1556512 T3 DK1556512 T3 DK 1556512T3 DK 03768790 T DK03768790 T DK 03768790T DK 1556512 T3 DK1556512 T3 DK 1556512T3
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- bgl6
- acid sequence
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- C12Y—ENZYMES
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- Bakery Products And Manufacturing Methods Therefor (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (21)
- BGL6 BETA-GLUCOSIDASE OG NUKLEINSYRER, DER KODER HERFOR PATENTKRAV1. Isoleret polynukleotid, der er afledt af en svampekilde, hvilket polynukleotid omfatter en nukleotidsekvens, der koder for et enzym, der har β-glukosidaseaktivitet, hvor det isolerede polynukleotid er udvalgt fra gruppen bestående af: (a) en nukleinsyresekvens, der koder for eller er komplementær til en sekvens, der koder for et BGL6-polypeptid, der er mindst 85 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (b) en nukleinsyresekvens, der koder for eller er komplementær til en sekvens, der koder for et BGL6-polypeptid, der er mindst 90 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (c) en nukleinsyresekvens, der koder for eller er komplementær til en sekvens, der koder for et BGL6-polypeptid, der er mindst 95 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (d) en nukleinsyresekvens, der koder for eller er komplementær til en sekvens, der koder for et BGL6-polypeptid, der har den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; og (e) en nukleinsyresekvens, der er præsenteret som SEQ ID NO: 3, eller komplementet deraf.
- 2. Isoleret polynukleotid ifølge krav 1, hvor %-identitet er beregnet ved hjælp af CLUSTAL-W-programmet i MacVector version 6,5, der er anvendt med standardparametre, herunder en open gap penalty på 10,0, en extended gap penalty på 0,1 og et BLOSUM 30 similaritetsmatrix.
- 3. Isoleret polynukleotid ifølge krav 1, hvor polynukleotidet er et RNA-molekyle.
- 4. Isoleret polynukleotid ifølge krav 2, der koder for et enzym, der har β-glukosidaseaktivitet, hvor enzymet er afledt af en Trichoderma-kilde.
- 5. Isoleret polynukleotid ifølge krav 3, hvor enzymet er afledt af Trichoderma reesei.
- 6. Ekspressionskonstrukt, der indbefatter en polynukleotidsekvens (i), der koder for et BGL6-polypeptid, der er mindst 85 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2, eller (ii) er komplementær til en nukleotidsekvens, der er mindst 85 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2.
- 7. Vektor, der indbefatter ekspressionskonstruktet ifølge krav 6.
- 8. Vektor, der omfatter et isoleret polynukleotid ifølge krav 1, det er operativt bundet til kontrolsekvenser, der er genkendt af en værtscelle, som er transformeret med vektoren.
- 9. Værtscelle, der er transformeret med vektoren ifølge krav 7.
- 10. Værtscelle, der er transformeret med vektoren ifølge krav 8.
- 11. Værtscelle ifølge krav 10, der er en prokaryotisk celle.
- 12. Værtscelle ifølge krav 10, der er en eukaryotisk celle.
- 13. Prokaryotisk rekombinant værtscelle, der omfatter et polynukleotid ifølge krav 1.
- 14. Isoleret BGL6-polypeptid med den biologiske aktivitet fra en β-glucosidase, der omfatter en sekvens, der er udvalgt fra gruppen bestående af: (a) en aminosyresekvens, der er mindst 85 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (b) en aminosyresekvens, der er mindst 90 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (c) en aminosyresekvens, der er mindst 95 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (d) en aminosyresekvens præsenteret som SEQ ID NO: 2; og (e) et isoleret fragment af den aminosyresekvens, der er præsenteret som SEQ ID NO: 2, der har en β-glucosidases biologiske aktivitet.
- 15. Fremgangsmåde til fremstilling af et BGL6-polypeptid, der har β-glukosidaseaktivitet, hvilken fremgangsmåde omfatter: (a) stabil transformering af en værtscelle med en ekspressionsvektor, der omfatter et polynukleotid som defineret ifølge krav 1; (b) dyrkning af den transformerede værtscelle under egnede forhold til, at værtscellen producerer β-glucosidasen; og (c) genvinding af β-glucosidasen.
- 16. Fremgangsmåde ifølge krav 15, hvor værtscellen er en filamentøs svampe- eller gærcelle.
- 17. Antisense-oligonukleotid komplementær til den bgl6-kodende sekvens af et messenger-RNA, der koder for et BGL6-polypeptid, der har den sekvens, der er præsenteret som SEQ ID NO: 2, hvor, ved eksponering for en β-glucosidase-producerende værtscelle, oligonukleotidet mindsker eller hæmmer produktionen af β-glucosidase gennem værtscellen.
- 18. Antisense-oligonukleotid ifølge krav 17, hvor værtscellen er en filamentøs svamp.
- 19. Detergentsammensætning, hvilken sammensætning omfatter et polypeptid med en β-glucosidases biologiske aktivitet udvalgt fra gruppen bestående af: (a) en aminosyresekvens, der er mindst 85 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (b) en aminosyresekvens, der er mindst 90 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (c) en aminosyresekvens, der er mindst 95 % sekvensidentisk med den aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (d) en aminosyresekvens, der er præsenteret som SEQ ID NO: 2; (e) et isoleret fragment af den aminosyresekvens, der er præsenteret som SEQ ID NO: 2, der har en β-glucosidases biologiske aktivitet.
- 20. Fremgangsmåde til forbedring af egenskaberne for en gærdej eller et bagt produkt, der er fremstillet af sådan en dej, hvilken fremgangsmåde i alt væsentligt består af følgende trin; (a) blanding af mindst omkring 10 ppm af en BGL6 ifølge krav 14 med dej ingredienser for at fremstille en dejblanding, og (b) bagning af dejblandingen for at fremstille et bagt produkt.
- 21. Fremgangsmåde til forbedring af en gærbrøddej eller gærbolledej eller gærbrød- eller gærbolleegenskaber, hvilken fremgangsmåde i alt væsentligt består af følgende trin: (a) blanding af mindst omkring 10 ppm af en BGL6 ifølge krav 14 med brød- eller bolledejsingredienser for at fremstille en dejblanding; (b) formning eller ilægning i forme af dejblandingen, (c) hævning af dejblandingen og (d) bagning af dejblandingen for at fremstille brød eller boller.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US42478402P | 2002-11-07 | 2002-11-07 | |
| PCT/US2003/035672 WO2004043980A2 (en) | 2002-11-07 | 2003-11-05 | Bgl6 beta-glucosidase and nucleic acids encoding the same |
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| DK1556512T3 true DK1556512T3 (da) | 2016-09-12 |
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| US (1) | US7582462B2 (da) |
| EP (1) | EP1556512B1 (da) |
| JP (1) | JP4744879B2 (da) |
| AU (1) | AU2003291395A1 (da) |
| CA (1) | CA2504744C (da) |
| DK (1) | DK1556512T3 (da) |
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- 2003-11-05 DK DK03768790.2T patent/DK1556512T3/da active
- 2003-11-05 ES ES03768790.2T patent/ES2601145T3/es not_active Expired - Lifetime
- 2003-11-05 WO PCT/US2003/035672 patent/WO2004043980A2/en not_active Ceased
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- 2003-11-05 JP JP2004551945A patent/JP4744879B2/ja not_active Expired - Fee Related
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| AU2003291395A8 (en) | 2004-06-03 |
| US7582462B2 (en) | 2009-09-01 |
| ES2601145T3 (es) | 2017-02-14 |
| JP4744879B2 (ja) | 2011-08-10 |
| EP1556512A4 (en) | 2006-05-24 |
| CA2504744A1 (en) | 2004-05-27 |
| EP1556512A2 (en) | 2005-07-27 |
| CA2504744C (en) | 2012-07-10 |
| JP2006505282A (ja) | 2006-02-16 |
| US20060258554A1 (en) | 2006-11-16 |
| WO2004043980A3 (en) | 2004-07-15 |
| AU2003291395A1 (en) | 2004-06-03 |
| WO2004043980A2 (en) | 2004-05-27 |
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