DK1675947T3 - Fremgangsmåde til screening for proteinsecernerende rekombinante værtsceller - Google Patents
Fremgangsmåde til screening for proteinsecernerende rekombinante værtscellerInfo
- Publication number
- DK1675947T3 DK1675947T3 DK04762918T DK04762918T DK1675947T3 DK 1675947 T3 DK1675947 T3 DK 1675947T3 DK 04762918 T DK04762918 T DK 04762918T DK 04762918 T DK04762918 T DK 04762918T DK 1675947 T3 DK1675947 T3 DK 1675947T3
- Authority
- DK
- Denmark
- Prior art keywords
- screening
- protein
- host cells
- recombinant host
- secreting
- Prior art date
Links
- 230000003248 secreting effect Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000012216 screening Methods 0.000 title abstract 3
- 230000000694 effects Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200301526 | 2003-10-16 | ||
| PCT/DK2004/000699 WO2005038024A1 (en) | 2003-10-16 | 2004-10-13 | A method of screening for protein secreting recombinant host cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1675947T3 true DK1675947T3 (da) | 2009-05-04 |
Family
ID=34442829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK04762918T DK1675947T3 (da) | 2003-10-16 | 2004-10-13 | Fremgangsmåde til screening for proteinsecernerende rekombinante værtsceller |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8846403B2 (da) |
| EP (1) | EP1675947B1 (da) |
| AT (1) | ATE420169T1 (da) |
| CA (1) | CA2540985A1 (da) |
| DE (1) | DE602004018959D1 (da) |
| DK (1) | DK1675947T3 (da) |
| WO (1) | WO2005038024A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010064748A1 (en) | 2008-12-04 | 2010-06-10 | Korea Research Institute Of Bioscience And Biotechnology | Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins |
| EP2496694B1 (en) | 2009-11-06 | 2017-04-19 | Novozymes, Inc. | Compositions for saccharification of cellulosic material |
| TW201118170A (en) * | 2009-11-20 | 2011-06-01 | Ind Tech Res Inst | Expression vector for expressing recombinant protein in cyanobacterium |
| US8986956B2 (en) | 2010-11-04 | 2015-03-24 | Korea Research Institute Of Bioscience And Biotechnology | Method for producing human epidermal growth factor in large volume from yeast |
| US8652804B2 (en) * | 2011-02-18 | 2014-02-18 | The Regents Of The University Of California | Transcription factor-based biosensors for detecting dicarboxylic acids |
| CN110241129B (zh) * | 2019-06-18 | 2022-11-15 | 山东大学 | 一种发光报告系统及其在抗生素发现筛选中的应用 |
| CN115976094B (zh) * | 2022-12-15 | 2024-02-20 | 浙江大学杭州国际科创中心 | 一种提高内源酶分泌的基因工程菌及其构建方法和应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUT76973A (hu) | 1994-09-16 | 1998-01-28 | Novartis Ag. | Eljárás hibásan hajtogatott fehérje kimutatására |
| EP1276857B1 (en) | 2000-04-07 | 2008-06-11 | Novozymes A/S | Signal sequence trapping |
-
2004
- 2004-10-13 US US10/574,625 patent/US8846403B2/en not_active Expired - Fee Related
- 2004-10-13 AT AT04762918T patent/ATE420169T1/de not_active IP Right Cessation
- 2004-10-13 DK DK04762918T patent/DK1675947T3/da active
- 2004-10-13 WO PCT/DK2004/000699 patent/WO2005038024A1/en not_active Ceased
- 2004-10-13 EP EP04762918A patent/EP1675947B1/en not_active Expired - Lifetime
- 2004-10-13 DE DE602004018959T patent/DE602004018959D1/de not_active Expired - Lifetime
- 2004-10-13 CA CA002540985A patent/CA2540985A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8846403B2 (en) | 2014-09-30 |
| DE602004018959D1 (de) | 2009-02-26 |
| CA2540985A1 (en) | 2005-04-28 |
| EP1675947B1 (en) | 2009-01-07 |
| ATE420169T1 (de) | 2009-01-15 |
| EP1675947A1 (en) | 2006-07-05 |
| US20070122812A1 (en) | 2007-05-31 |
| WO2005038024A1 (en) | 2005-04-28 |
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