DK2078071T3 - Rationelt udformede cellekulturmedier - Google Patents
Rationelt udformede cellekulturmedier Download PDFInfo
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- DK2078071T3 DK2078071T3 DK07868688.8T DK07868688T DK2078071T3 DK 2078071 T3 DK2078071 T3 DK 2078071T3 DK 07868688 T DK07868688 T DK 07868688T DK 2078071 T3 DK2078071 T3 DK 2078071T3
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- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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Claims (14)
1. Cellekulturmedium omfattende mellem 7 mM og 30 mM leucin, mellem 7 mM og 30 mM lysin, mellem 7 mM og 30 mM threonin, mellem 7 mM og 30 mM prolin og mellem 7 mM og 30 mM valin.
2. Cellekulturmedium ifølge krav 1, som endvidere omfatter mere end eller lig med 3 mM tyrosin.
3. Cellekulturmedium ifølge krav 1 eller 2, hvori den kombinerede koncentration af leucin, lysin, threonin, prolin og valin ligger mellem 60% og 80% af koncentrationen af de samlede essentielle aminosyrer i cellekulturmediet.
4. Cellekulturmedium ifølge krav 1 eller 2, hvori den kombinerede koncentration af de essentielle aminosyrer ligger mellem 30% og 50% af koncentrationen af de samlede aminosyrer.
5. Cellekulturmedium ifølge et hvilket som helst af kravene 1 til 4 omfattende en totalkoncentration af aminosyrer mellem 120 mM og 350 mM.
6. Cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6 omfattende en totalkoncentration af aminosyrer på mere end 140 mM.
7. Fremgangsmåde til fremstilling af et polypeptid i en cellekultur omfattende: (1) tilvejebringelse af en cellekultur, der omfatter: a. celler, der omfatter en nukleinsyre, som koder for et polypeptid af interesse; og b. et cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6; og (2) opretholdelse af cellekulturen under betingelser, der tillader ekspression af polypeptidet af interesse.
8. Fremgangsmåde ifølge krav 7, som endvidere omfatter en fremgangsmåde til udvinding af et renset polypeptid fra en indføringsvæske omfattende følgende trin: at lade indføringsvæsken passere gennem et medium i en søjle under driftsbetingelser, der bringer mediet til at binde mindst 2,8 mg polypeptid pr. ml medium, idet mediet er valgt fra den gruppe, der består af et ladet ionbytter- medium, en hydrofob vekselvirkningschromatografiharpiks og en immobiliseret metalaffinitetschromatografiharpiks; og at udvinde det rensede polypeptid fra det fra søjlen udstrømmende materiale.
9. Fremgangsmåde ifølge krav 7, som endvidere omfatter en fremgangsmåde til udvinding af et renset polypeptid fra en indføringsvæske omfattende følgende trin: at lade indføringsvæsken passere gennem et medium i en søjle under driftsbetingelser, der er defineret ved en fordelingskoefficient på mindst 0,1; og at udvinde den rensede polypeptid fra det fra søjlen udstrømmende materiale.
10. Fremgangsmåde til fremstilling af et polypeptid i en cellekultur omfattende: (1) tilvejebringelse af en cellekultur, der omfatter: a. celler, der omfatter en nukleinsyre, som koder for et polypeptid af interesse; og b. et start-cellekulturmedium, idet start-cellekulturmediets rumfang er 60-99% af et ønsket cellekulturmedierumfang; (2) tilvejebringelse af et cellekulturfodringsmedium til cellekulturen ifølge trin (1), idet cellekulturfodringsmediets rumfang er 1-40% af det ønskede cellekulturmedierumfang, og idet det fremkomne ønskede cellekulturmedium er et cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6; og (3) opretholdelse af cellekulturen under betingelser, der tillader ekspression af polypeptidet af interesse.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 10, ved hvilken cellekulturen er en cellekultur i stor målestok.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 11, ved hvilken cellerne er dyreceller.
13. Fremgangsmåde ifølge et hvilket som helst af kravene 7 og 10 til 12, ved hvilken polypeptidet renses.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 13, ved hvilken mediet er et defineret medium.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85828906P | 2006-11-08 | 2006-11-08 | |
| PCT/US2007/083947 WO2008063892A2 (en) | 2006-11-08 | 2007-11-07 | Rationally designed media for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2078071T3 true DK2078071T3 (da) | 2015-04-20 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07868688.8T DK2078071T3 (da) | 2006-11-08 | 2007-11-07 | Rationelt udformede cellekulturmedier |
Country Status (25)
| Country | Link |
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| US (2) | US8232075B2 (da) |
| EP (2) | EP2921552A1 (da) |
| JP (2) | JP5564259B2 (da) |
| KR (1) | KR101523782B1 (da) |
| CN (2) | CN101535469A (da) |
| AR (1) | AR063623A1 (da) |
| AU (1) | AU2007323978B2 (da) |
| BR (1) | BRPI0718713B1 (da) |
| CA (1) | CA2668771C (da) |
| CL (1) | CL2007003221A1 (da) |
| CR (1) | CR10781A (da) |
| DK (1) | DK2078071T3 (da) |
| EC (1) | ECSP099313A (da) |
| ES (1) | ES2538986T3 (da) |
| IL (1) | IL198486A (da) |
| MX (1) | MX2009004974A (da) |
| NO (1) | NO20091607L (da) |
| PE (1) | PE20081655A1 (da) |
| PL (1) | PL2078071T3 (da) |
| PT (1) | PT2078071E (da) |
| RU (1) | RU2520810C2 (da) |
| SI (1) | SI2078071T1 (da) |
| TW (1) | TW200827445A (da) |
| WO (1) | WO2008063892A2 (da) |
| ZA (1) | ZA200903170B (da) |
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| SI2078071T1 (sl) | 2006-11-08 | 2015-05-29 | Wyeth Llc | Racionalno zasnovana gojišča za celično kulturo |
| WO2008136398A1 (ja) | 2007-04-26 | 2008-11-13 | Chugai Seiyaku Kabushiki Kaisha | 高濃度アミノ酸含有培地を用いた細胞の培養方法 |
| ES2657055T3 (es) | 2007-08-09 | 2018-03-01 | Wyeth Llc | Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores |
| CN102112595B (zh) * | 2008-08-06 | 2014-11-26 | 普莱克斯技术有限公司 | 用于控制哺乳动物细胞培养过程的系统和方法 |
| US8178318B2 (en) * | 2008-08-06 | 2012-05-15 | Praxair Technology, Inc. | Method for controlling pH, osmolality and dissolved carbon dioxide levels in a mammalian cell culture process to enhance cell viability and biologic product yield |
| EP3309168A1 (en) | 2009-08-06 | 2018-04-18 | F. Hoffmann-La Roche AG | Method to improve virus removal in protein purification |
| US20110262965A1 (en) * | 2010-04-23 | 2011-10-27 | Life Technologies Corporation | Cell culture medium comprising small peptides |
| CA2797356C (en) * | 2010-04-26 | 2020-12-29 | Novartis Ag | Improved cell culture medium |
| PL2563906T3 (pl) | 2010-04-26 | 2018-04-30 | Novartis Ag | Sposób hodowli komórek cho |
| EP2447717B1 (en) | 2010-10-27 | 2013-09-18 | Lonza Biologics plc. | Rapid method for targeted cell (line) selection |
| PT105484A (pt) | 2011-01-14 | 2012-07-16 | Univ Nova De Lisboa | Um método de ambientómica funcional para engenharia de meio de cultura celular |
| PT2837680T (pt) | 2011-07-01 | 2020-04-17 | Amgen Inc | Cultura celular de mamífero |
| CN103717729B (zh) * | 2011-07-08 | 2017-11-21 | 动量制药公司 | 细胞培养方法 |
| SI3395423T1 (sl) * | 2013-03-14 | 2023-12-29 | Amgen Inc. | Odstranjevanje izluženega liganda za afinitetno čiščenje |
| AR095196A1 (es) * | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| TWI870789B (zh) | 2015-08-04 | 2025-01-21 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法 |
| EP3377613A1 (en) | 2015-11-17 | 2018-09-26 | Pfizer Inc | Media and fermentation methods for producing polysaccharides in bacterial cell culture |
| WO2017175086A1 (en) * | 2016-04-05 | 2017-10-12 | Pfizer Inc. | Cell culture process |
| CN106096077B (zh) * | 2016-05-26 | 2019-05-21 | 新乡医学院 | 培养基优化方法 |
| JP6314201B1 (ja) * | 2016-11-21 | 2018-04-18 | テラファーマ株式会社 | 樹状細胞洗浄液及びこれを用いた樹状細胞の洗浄方法、並びに樹状細胞含有組成物の調製方法 |
| JP7148524B2 (ja) * | 2017-02-08 | 2022-10-05 | ファイザー・インク | キャップされたおよびキャップされていない抗体システインのための大規模産生プロセス、ならびに治療的タンパク質コンジュゲーションにおけるその使用 |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| AU2018350890B2 (en) * | 2017-10-16 | 2024-07-04 | Regeneron Pharmaceuticals, Inc. | In situ Raman spectroscopy systems and methods for controlling process variables in cell cultures |
| WO2019099891A1 (en) * | 2017-11-16 | 2019-05-23 | Life Technologies Corporation | Streamlined methods for making liquid media |
| JP6385607B2 (ja) * | 2018-02-13 | 2018-09-05 | テラファーマ株式会社 | 樹状細胞ワクチン |
| CN108823173A (zh) * | 2018-07-24 | 2018-11-16 | 郑州伊美诺生物技术有限公司 | 用于杂交瘤细胞培养的补料培养基及其制备方法 |
| CN109576203B (zh) * | 2018-12-20 | 2021-03-23 | 吉林省浦生泰生物技术有限责任公司 | 一种基于中药添加剂的复合益生菌培养基及其制备方法 |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| CN111748527B (zh) * | 2020-05-15 | 2021-01-29 | 上海多宁生物科技有限公司 | 化学成分限定高效补料培养基及其制备方法和应用 |
| CN113450882B (zh) * | 2020-09-27 | 2022-03-01 | 深圳太力生物技术有限责任公司 | 一种基于人工智能的基础培养基配方开发方法及系统 |
| JP7822149B2 (ja) | 2020-10-02 | 2026-03-02 | ファイザー・インク | Ｒｓｖ ｆタンパク質生産のための細胞培養工程 |
| GB202105424D0 (en) * | 2021-04-16 | 2021-06-02 | UCB Biopharma SRL | Cell culture processes |
| WO2022254319A1 (en) | 2021-06-01 | 2022-12-08 | Pfizer Inc. | Cell culture method for producing sfgfr3 polypeptide |
| JP7190534B1 (ja) * | 2021-06-14 | 2022-12-15 | アサヒ飲料株式会社 | 微生物の検出方法 |
| JP7820943B2 (ja) * | 2021-10-28 | 2026-02-26 | 花王株式会社 | Ｖｈｈ抗体の製造方法 |
| EP4582528A4 (en) * | 2022-10-17 | 2025-12-17 | Phc Holdings Corp | CROP CONTROL DEVICE AND CROP CONTROL METHOD |
| CN116334007A (zh) * | 2023-04-10 | 2023-06-27 | 珠海市丽珠单抗生物技术有限公司 | 一种慢病毒的包装方法 |
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| TWI384069B (zh) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| US7335491B2 (en) * | 2004-08-27 | 2008-02-26 | Wyeth Research Ireland Limited | Production of anti-abeta |
| EP1851305B1 (en) | 2005-02-11 | 2012-01-18 | Novo Nordisk Health Care AG | Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate |
| EP1869065B1 (en) | 2005-03-11 | 2020-05-06 | Wyeth LLC | A method of weak partitioning chromatography |
| SI2078071T1 (sl) | 2006-11-08 | 2015-05-29 | Wyeth Llc | Racionalno zasnovana gojišča za celično kulturo |
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- 2007-11-07 AU AU2007323978A patent/AU2007323978B2/en not_active Ceased
- 2007-11-07 EP EP15159383.7A patent/EP2921552A1/en not_active Withdrawn
- 2007-11-07 EP EP07868688.8A patent/EP2078071B1/en not_active Revoked
- 2007-11-07 PT PT78686888T patent/PT2078071E/pt unknown
- 2007-11-07 RU RU2009114855/10A patent/RU2520810C2/ru active
- 2007-11-07 MX MX2009004974A patent/MX2009004974A/es active IP Right Grant
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- 2007-11-08 CL CL200703221A patent/CL2007003221A1/es unknown
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