DK2139986T3 - Anvendelse af lav temperatur og/eller lav pH-værdi i cellekultur - Google Patents
Anvendelse af lav temperatur og/eller lav pH-værdi i cellekultur Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (9)
1. Fremgangsmåde til fremstilling af et protein i en cellekultur omfattende: (a) at dyrke celler i cellekulturen ved en reduceret temperatur, hvor den reducerede temperatur ligger i området fra 27,0°C til mindre end 30,0°C; og (b) at dyrke celler i cellekulturen ved en reduceret pH-værdi, hvor den reducerede pH-værdi ligger i et område på 6,80 til mindre end 7,00; for at reducere produktionen af forkert foldede proteiner og/eller aggregerede proteiner, hvor cellekulturen er en pattedyr-cellekultur, hvor det producerede protein er en opløselig receptor, hvor den opløselige receptor er TNFR-Fc eller slL-13R.
2. Fremgangsmåde ifølge krav 1, hvor cellekulturen er en CHO-cellekultur.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor den opløselige receptor er TNFR-Fc.
4. Fremgangsmåde ifølge krav 3, hvor den reducerede temperatur er 29,5 °C.
5. Fremgangsmåde ifølge krav 3 eller 4, hvor den reducerede pH-værdi er 6,95.
6. Fremgangsmåde ifølge krav 1, hvor den opløselige receptor er sll_-13R
7. Fremgangsmåde ifølge et af kravene 1-6, yderligere omfattende et trin, hvor proteinet isoleres fra cellekulturen.
8. Fremgangsmåde ifølge krav 7, hvor proteinet endvidere oprenses eller forarbejdes til formulering.
9. Fremgangsmåde ifølge krav 8, hvor proteinet formuleres til en farmaceutisk sammensætning.
Applications Claiming Priority (2)
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|---|---|---|---|
| US91338207P | 2007-04-23 | 2007-04-23 | |
| PCT/US2008/061123 WO2008131374A1 (en) | 2007-04-23 | 2008-04-22 | Use of low temperature and/or low ph in cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2139986T3 true DK2139986T3 (da) | 2017-10-02 |
Family
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| DK08746526.6T DK2139986T3 (da) | 2007-04-23 | 2008-04-22 | Anvendelse af lav temperatur og/eller lav pH-værdi i cellekultur |
| DK08746528.2T DK2139987T5 (da) | 2007-04-23 | 2008-04-22 | Fremgangsmåder til proteinfremstilling med anti-senescens-forbindelser |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
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| DK08746528.2T DK2139987T5 (da) | 2007-04-23 | 2008-04-22 | Fremgangsmåder til proteinfremstilling med anti-senescens-forbindelser |
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| EP (2) | EP2139987B9 (da) |
| JP (3) | JP5557736B2 (da) |
| KR (3) | KR101560421B1 (da) |
| CN (3) | CN101668845B (da) |
| AR (2) | AR066238A1 (da) |
| AU (2) | AU2008242632B2 (da) |
| BR (2) | BRPI0810511A2 (da) |
| CA (2) | CA2684727C (da) |
| CL (2) | CL2008001156A1 (da) |
| CO (2) | CO6241165A2 (da) |
| DK (2) | DK2139986T3 (da) |
| ES (2) | ES2646090T3 (da) |
| HK (1) | HK1216261A1 (da) |
| HU (1) | HUE034776T2 (da) |
| IL (2) | IL201720A (da) |
| MX (2) | MX2009011363A (da) |
| PA (2) | PA8777701A1 (da) |
| PE (2) | PE20090731A1 (da) |
| PL (2) | PL2139986T3 (da) |
| PT (2) | PT2139986T (da) |
| RU (2) | RU2009138226A (da) |
| SI (2) | SI2139987T1 (da) |
| TW (2) | TW200902708A (da) |
| WO (2) | WO2008131374A1 (da) |
| ZA (1) | ZA200907426B (da) |
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| ES2657055T3 (es) | 2007-08-09 | 2018-03-01 | Wyeth Llc | Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores |
| US8937046B2 (en) | 2008-09-22 | 2015-01-20 | The Regents Of The University Of Colorado, A Body Corporate | Modulating the alternative complement pathway |
| NZ592095A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography |
| BRPI0920572A8 (pt) | 2008-10-20 | 2015-10-27 | Abbott Lab | Inativação viral durante a purificação dos anticorpos |
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| PL2563906T3 (pl) | 2010-04-26 | 2018-04-30 | Novartis Ag | Sposób hodowli komórek cho |
| CN103282509A (zh) * | 2010-12-28 | 2013-09-04 | 中外制药株式会社 | 动物细胞的培养方法 |
| US9062106B2 (en) | 2011-04-27 | 2015-06-23 | Abbvie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| EP2718328A4 (en) | 2011-06-08 | 2014-12-24 | Acceleron Pharma Inc | COMPOSITIONS AND METHODS FOR INCREASING THE HALF TIME OF SERUM |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
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| HK1207960A1 (en) | 2013-03-12 | 2016-02-19 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
| WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| AU2014233393B2 (en) | 2013-03-15 | 2020-05-28 | Genentech, Inc. | Cell culture compositions with antioxidants and methods for polypeptide production |
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