DK2119771T3 - Proteiner - Google Patents

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Publication number
DK2119771T3
DK2119771T3 DK09011127.9T DK09011127T DK2119771T3 DK 2119771 T3 DK2119771 T3 DK 2119771T3 DK 09011127 T DK09011127 T DK 09011127T DK 2119771 T3 DK2119771 T3 DK 2119771T3
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DK
Denmark
Prior art keywords
ala
seq
leu
gly
amino acid
Prior art date
Application number
DK09011127.9T
Other languages
English (en)
Inventor
Jorn Borch Soe
Jorn Dalgaard Mikkelsen
Rikke Hoegh Lorentsen
Smith Anja Hemmingsen Kellett
Janne Brunstedt
Jens Frisbaek Sorensen
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0330016.7A external-priority patent/GB0330016D0/en
Priority claimed from PCT/IB2004/000655 external-priority patent/WO2004064537A2/en
Priority claimed from US10/911,160 external-priority patent/US20050196766A1/en
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Application granted granted Critical
Publication of DK2119771T3 publication Critical patent/DK2119771T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01085—Fatty-acid synthase (2.3.1.85)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Claims (8)

  1. DK/EP 2119771 T3 - 1 - PROTEINER
    1. Variantlipidacyltransferase med øjet phospholipidtransferaseaktivitet, hvor enzymet omfatter aminosyresekvensmotivet GDSX, hvor X er én eller flere af følgende aminosyrerester L, A, V, I, F, Y, H, 5 Q, T, N, M eller S; hvor variantenzynict omfatter én eller flere aminosyremodifikationer sammenlignet med en modersekvens ved en hvilken som helst eller flere af aminosyreresteme: S3N, Q, K, R, P eller M; eller E309A; hvor nummereringen er den, der er opnået fra alignment af variantsekvensen med 10 referencesekvensen vist som SEQ ID NO 2 og hvor moderlipidacyltransferasen omfatter aminosyresekvensen vist som SEQ ID NO 28 eller en aminosyresekvens, der har 75 % eller større identitet med SEQ ID NO 28 eller hvor moderlipidacyltransferasen er kodet for af nukleotidsekvensen vist som SEQ ID NO 29, eller en nukleotidsekvens, der har mindst 75 % eller større identitet med sekvensen vist som SEQ ID NO 29.
  2. 2. Fremgangsmåde til fremstilling af en fødevare, hvilken fremgangsmåde omfatter tilsætning af et variantlipidacyltransferaseenzym ifølge krav 1 til én eller flere af fødevarens ingredienser.
  3. 3. Fremgangsmåde til fremstilling af et bagt produkt ud af en dej, hvilken fremgangsmåde omfatter tilsætning af et variantlipidacyltransferaseenzym ifølge krav 1 til dejen.
  4. 4. Anvendelse af et variantlipidacyltransferaseenzym ifølge krav 1 i en fremgangsmåde til behandling 20 af æg eller ægbaserede produkter for at fremstille lysophospholipider.
  5. 5. Fremgangsmåde til enzymatisk degummiering af vegetabilske eller spiselige olier, hvilken fremgangsmåde omfatter behandling af den spiselige olie eller den vegetabilske olie med et variantlipidacyltransferaseenzym ifølge krav 1 for således at hydrolysere størstedelen af de polære lipider (f.eks. phospholipid og/eller glycolipid).
  6. 6. Anvendelse af et variantlipidacyltransferaseenzym ifølge krav 1 i en fremgangsmåde til reduktion af indholdet af et phospholipid i en spiselig olie, hvilken fremgangsmåde omfatter behandling af olien med variantlipidacyltransferaseenzymet for således at hydrolysere størstedelen af phospholipidet, og separering af en vandig fase, der indeholder det hydrolyserede phospholipid fra olien.
  7. 7. Anvendelse af et variantlipidacyltransferaseenzym ifølge krav 1 i biokonversionen af polære lipider 30 (fortrinsvis glycolipider) for at fremstille produkter af høj værdi, såsom carbohydratestere og/eller proteinestere og/eller proteinunderenhedsestere og/eller en hydroxysyreester.
  8. 8. Immobiliseret variantlipidacyltransferaseenzym ifølge krav 1.
DK09011127.9T 2003-12-24 2004-12-23 Proteiner DK2119771T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB0330016.7A GB0330016D0 (en) 2003-12-24 2003-12-24 Method
PCT/IB2004/000655 WO2004064537A2 (en) 2003-01-17 2004-01-15 Method for the in situ production of an emulsifier in foodstuff
GBGB0415999.2A GB0415999D0 (en) 2003-12-24 2004-07-16 Proteins
US10/911,160 US20050196766A1 (en) 2003-12-24 2004-08-02 Proteins
EP04806537.9A EP1704236B1 (en) 2003-12-24 2004-12-23 Proteins

Publications (1)

Publication Number Publication Date
DK2119771T3 true DK2119771T3 (da) 2018-12-10

Family

ID=34753781

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09011127.9T DK2119771T3 (da) 2003-12-24 2004-12-23 Proteiner

Country Status (8)

Country Link
EP (2) EP2119771B1 (da)
JP (1) JP5697294B2 (da)
KR (1) KR101169265B1 (da)
AU (1) AU2004312215B2 (da)
BR (1) BRPI0417533B1 (da)
CA (1) CA2550800A1 (da)
DK (1) DK2119771T3 (da)
WO (1) WO2005066347A1 (da)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406723B1 (en) 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
WO2002094123A2 (en) 2001-05-18 2002-11-28 Danisco A/S Method of preparing a dought with an enzime
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
EP1704240B1 (en) * 2003-12-24 2007-10-24 Danisco A/S Enzymatic treatment of oils
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
MY153898A (en) 2005-06-22 2015-04-15 Plexxikon Inc Compounds and methods for kinase modulation, and indications therefor
JP4953046B2 (ja) * 2005-07-29 2012-06-13 独立行政法人産業技術総合研究所 ランダムペプチドライブラリーもしくは抗体超可変領域を模倣したペプチドライブラリーと、ｒｎａ結合タンパク質を用いる試験管内ペプチド選択法を組み合わせた、新規の機能性ペプチド創製システム
EP2069488A2 (en) 2006-10-03 2009-06-17 Novozymes A/S Lipolytic enzyme variants
WO2008063888A2 (en) 2006-11-22 2008-05-29 Plexxikon, Inc. Compounds modulating c-fms and/or c-kit activity and uses therefor
WO2008079909A1 (en) 2006-12-21 2008-07-03 Plexxikon, Inc. Pyrrolo [2,3-b] pyridines as kinase modulators
CA2673954C (en) 2007-01-25 2015-09-15 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
EP2121909A1 (en) * 2007-02-27 2009-11-25 Danisco US Inc. Cleaning enzymes and fragrance production
CA2678760A1 (en) * 2007-02-27 2008-09-04 Danisco Us Inc. Cleaning enzymes and malodor prevention
JP2010533729A (ja) 2007-07-17 2010-10-28 プレキシコン，インコーポレーテッド キナーゼ調節のための化合物と方法、及びそのための適応
EP2235151B1 (en) 2007-12-21 2012-09-12 DuPont Nutrition Biosciences ApS Process for edible oil refining using a lipid acyltransferase
GB0807161D0 (en) 2008-04-18 2008-05-21 Danisco Process
US20120028318A1 (en) 2009-03-18 2012-02-02 Danisco Us Inc. Fungal cutinase from magnaporthe grisea
CA2738573C (en) 2009-04-03 2013-02-19 F. Hoffmann-La Roche Ag Compositions of propane-1 sulfonic acid {3-[5-(4-chloro-phenyl)-1h-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluoro-phenyl}-amide and uses thereof
EP2438823A4 (en) 2009-06-05 2014-06-11 Ajinomoto Kk ENZYME PREPARATION AGENT FOR THE RECONSTITUTION OF PROCESSED PRODUCTS OF LIVESTOCK MEAT AND PROCESS FOR THE MANUFACTURE OF PROCESSED BEETMEAT PRODUCTS
US8329724B2 (en) 2009-08-03 2012-12-11 Hoffmann-La Roche Inc. Process for the manufacture of pharmaceutically active compounds
EP2488638A1 (en) 2009-10-15 2012-08-22 DuPont Nutrition Biosciences ApS Lipid acyltransferase proteins and methods of making them
JP2013510166A (ja) 2009-11-06 2013-03-21 プレキシコン インコーポレーテッド キナーゼ調節のための化合物、方法およびその適用
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011084417A1 (en) 2009-12-21 2011-07-14 Danisco Us Inc. Detergent compositions containing geobacillus stearothermophilus lipase and methods of use thereof
US20120258507A1 (en) 2009-12-21 2012-10-11 Danisco Us Inc. Detergent compositions containing thermobifida fusca lipase and methods of use thereof
BR112012017056A2 (pt) 2009-12-21 2016-11-22 Danisco Us Inc "composições detergentes contendo lipase de bacillus subtilis e métodos para uso das mesmas"
GB201007668D0 (en) 2010-05-07 2010-06-23 Danisco Method
AR081423A1 (es) 2010-05-28 2012-08-29 Danisco Us Inc Composiciones detergentes con contenido de lipasa de streptomyces griseus y metodos para utilizarlas
EP2665825B1 (en) 2011-01-21 2016-01-13 Novozymes A/S Production of fatty acid alkyl esters
PE20141360A1 (es) 2011-02-07 2014-10-13 Plexxikon Inc Compuestos y metodos para la modulacion de quinasas e indicaciones para los mismos.
TWI558702B (zh) 2011-02-21 2016-11-21 普雷辛肯公司 醫藥活性物質的固態形式
US9150570B2 (en) 2012-05-31 2015-10-06 Plexxikon Inc. Synthesis of heterocyclic compounds
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
WO2017060471A1 (en) 2015-10-09 2017-04-13 Novozymes A/S Enzymatic or non-enzymatic biodiesel polishing process
WO2017216382A1 (en) 2016-06-16 2017-12-21 Novozymes A/S Reduction of phospholipids in phospholipid-containing oil material
EA202092799A1 (ru) 2018-05-22 2021-03-01 ДСМ АйПи АССЕТС Б.В. Модифицированные стерол-ацилтрансферазы
KR20240005689A (ko) 2021-05-04 2024-01-12 노보자임스 에이/에스 수소처리 식물성 오일(hvo) 제조를 위한 공급원료의 효소 처리

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA92037A (en) 1904-11-25 1905-03-07 The Hooven, Owens, Rentchler Company Turbine bearings
GB330016A (en) 1929-03-03 1930-06-05 Thomas Reeder Ltd Improvements in and relating to flanges for warp beams or rollers for use in textile and other machines
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
KR100225087B1 (ko) 1990-03-23 1999-10-15 한스 발터라벤 피타아제의 식물내 발현
ES2068586T5 (es) 1990-05-09 2004-12-01 Novozymes A/S Una preparacion de celulasa que comprende una enzima endoglucanasa.
DE4112440C1 (da) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
EP0594612B2 (en) 1991-05-03 2010-02-24 Raisio Nutrition Ltd. Process for the preparation of a substance comprising a beta-sitostanol fatty acid ester or a mixture thereof
WO1994013820A1 (en) 1992-12-10 1994-06-23 Gist-Brocades N.V. Production of heterologous proteins in filamentous fungi
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5741665A (en) 1994-05-10 1998-04-21 University Of Hawaii Light-regulated promoters for production of heterologous proteins in filamentous fungi
US6406723B1 (en) * 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
DE69611102T2 (de) 1995-09-22 2001-06-21 Amersham Pharmacia Biotech Uk Ltd., Little Chalfont Verstärkung der mutagenese von nukleinsäuren
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6344328B1 (en) 1995-12-07 2002-02-05 Diversa Corporation Method for screening for enzyme activity
ATE231186T1 (de) * 1998-07-21 2003-02-15 Danisco Lebensmittel
DK1131416T3 (da) 1998-11-27 2009-10-26 Novozymes As Lipolytiske enzymvarianter
JP2004500019A (ja) 1999-03-26 2004-01-08 ディベルサ　コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
DE60045117D1 (de) 1999-08-30 2010-11-25 Monsanto Technology Llc Pflanzen-Sterol-Acyltransferasen
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
AU2001278918A1 (en) 2000-07-13 2002-01-30 Maxygen, Inc. Novel lipase genes
AU2001279254A1 (en) 2000-08-11 2002-02-25 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
AU2002233168B2 (en) 2001-02-21 2007-02-15 Novozymes A/S Production of starchy food products
DE50115482D1 (de) * 2001-07-11 2010-06-24 Cognis Ip Man Gmbh Lipase/Acyltransferase aus Candida parapsilosis
EP2290085B1 (en) * 2003-01-17 2017-12-13 DuPont Nutrition Biosciences ApS Method for the in situ production of an emulsifier in a foodstuff
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
MXPA06001719A (es) * 2003-08-11 2006-05-19 Codexis Inc Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados.

Also Published As

Publication number Publication date
JP5697294B2 (ja) 2015-04-08
AU2004312215A1 (en) 2005-07-21
AU2004312215B2 (en) 2009-03-05
CA2550800A1 (en) 2005-07-21
BRPI0417533B1 (pt) 2016-03-01
BRPI0417533A (pt) 2007-03-20
WO2005066347A1 (en) 2005-07-21
EP2119771B1 (en) 2018-08-22
EP1704236B1 (en) 2017-11-29
JP2007516717A (ja) 2007-06-28
EP2119771A3 (en) 2010-01-27
KR20060111496A (ko) 2006-10-27
EP2119771A2 (en) 2009-11-18
KR101169265B1 (ko) 2012-08-02
EP1704236A1 (en) 2006-09-27

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