DK2278029T3 - Fremgangsmåder til undersøgelse og genetisk analyse af populationer - Google Patents

Fremgangsmåder til undersøgelse og genetisk analyse af populationer Download PDF

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DK2278029T3
DK2278029T3 DK10012061.7T DK10012061T DK2278029T3 DK 2278029 T3 DK2278029 T3 DK 2278029T3 DK 10012061 T DK10012061 T DK 10012061T DK 2278029 T3 DK2278029 T3 DK 2278029T3
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DK10012061.7T
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Matthew Ashby
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Taxon Biosciences Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
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    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/40—Population genetics; Linkage disequilibrium
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Claims (14)

  1. FREMGANGSMÅDER TIL UNDERSØGELSE OG GENETISK ANALYSE AF POPULATIONER PATENTKRAV
    1. Fremgangsmåde til identificering af én eller flere rRNA-markører fra et antal af prøver, hvor hyppigheden af rRNA-markøreme korrelerer med hyppigheden af et prøveparameter i antallet af prøver, hvilken fremgangsmåde omfatter trinnene med: a) tilvejebringelse af en markørdiversitetsprofil- (MDP) database, der omfatter et antal MDP’er, der er afledt fra et antal prøver, hvor hver MDP er afledt fra en prøve, hvor hver MDP omfatter hyppigheden af et antal rRNA-markører, der er isoleret fra en prøve, og hvor hver af rRNA-markøreme omfatter en polymorf sekvens af et mikrobielt rRNA-gen; b) tilvejebringelse af hyppigheden af et prøveparameter i hver af antallet af prøver, c) udførelse af en korreleringsanalyse af prøveparameterhyppigheden i en prøve med hyppigheden af en rRNA-markør i MDP svarende til den samme prøve, på tværs af antallet af prøver, d) gentagelse af trin c) med mindst én anden rRNA-markør; og e) identificering af mindst én rRNA-markør, hvis hyppighed korrelerer med prøveparameteret.
  2. 2. Fremgangsmåde ifølge krav 1, hvor antallet af rRNA-markører omfatter hver rRNA-markør i MDP-databasen.
  3. 3. Fremgangsmåde ifølge krav 1, hvor antallet af rRNA-markører omfatter rRNA-markøreme, der er udvalgt i fremgangsmåden ifølge krav 1.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor korreleringen kan udtrykkes ved en korreleringsværdi, der er udvalgt fra gruppen bestående af mindst 0,5, mindst 0,6, mindst 0,7, mindst 0,8, mindst 0,9 og mindst 0,95.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor rRNA-markørhyppigheden er en hyppighed i forhold til den samlede hyppighed af antallet af rRNA-markører i prøven.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, hvor det mikrobielle rRNA-gen er et 16S rRNA-gen.
  7. 7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, hvor den polymorfe sekvens af et mikrobielt rRNA-gen er fra den intergeniske region mellem et 16S rRNA-gen og et 23 S rRNA-gen.
  8. 8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor prøven er udvalgt fra gruppen bestående af: en jordprøve, en stenprøve, en vandprøve, en luftprøve, en carbonhydridprøve, en petroleumsprøve og en biofilmprøve.
  9. 9. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 8, hvor prøven er opnået fra gruppen bestående af: petroleum, carbonhydrider, et oliereservoir, et gasreservoir, jord, sten, vand, luft, en bygning og en vej.
  10. 10. Fremgangsmåde ifølge krav 9, hvor prøveparameteret er udvalgt fra gruppen bestående af: petroleum, carbonhydrider, olie, gas, en uorganisk forbindelse, et mineral, pH, temperatur og saltholdighed.
  11. 11. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor prøven er opnået fra en udvælgelse af gruppen bestående af: et menneske, en plante, et dyr, et foderstof, en cellekultur og en vævskultur.
  12. 12. Fremgangsmåde ifølge krav 11, hvor prøveparameteret er udvalgt fra gruppen bestående af; en akut sygdomstilstand, en kronisk sygdomstilstand, en fysiologisk tilstand og et udviklingstrin.
  13. 13. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor prøveparameteret er tid, og hvor antallet af prøver omfatter et tidsforløb.
  14. 14. Fremgangsmåde ifølge krav 13, hvor antallet af prøver omfatter et tidsforløb, der er anvendeligt til at overvåge miljømæssige genopretningsbestræbelser eller oliefeltfremstilling.
DK10012061.7T 2000-04-10 2001-04-10 Fremgangsmåder til undersøgelse og genetisk analyse af populationer DK2278029T3 (da)

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US19606300P 2000-04-10 2000-04-10
US19625800P 2000-04-11 2000-04-11
EP10152284.5A EP2206791B1 (en) 2000-04-10 2001-04-10 Methods for the survey and genetic analysis of populations

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