DK1907571T3 - Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter - Google Patents

Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter Download PDF

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DK1907571T3
DK1907571T3 DK06760745.7T DK06760745T DK1907571T3 DK 1907571 T3 DK1907571 T3 DK 1907571T3 DK 06760745 T DK06760745 T DK 06760745T DK 1907571 T3 DK1907571 T3 DK 1907571T3
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Radoje Drmanac
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Claims (33)

1. Fremgangsmåde til sekventering af et eller flere målpolynukleotider, hvilken fremgangsmåde omfatter trinene: tilfældig fragmentering af den ene eller de flere målpolynukleotider, der er til stede, i en forudbestemt dækningsmængde til frembringelse af en population, der indeholder overlappende fragmenter med en første størrelse, der hver har en gennemsnitslængde, der er mindre end gennemsnitslængderne af målpolynukleotiderne; frembringelse af et antal separate blandinger fra populationen af fragmenter med en første størrelse, idet et sådant antal vælges, således at mindst 60 % af sådanne separate blandinger kun indeholder ikke-overlappende fragmenter, og således at udgangsblandingen for hvert sådant fragment med en første størrelse er bestemmelig; sekventering af mindst en del af et eller flere fragmenter med en første størrelse i hver separate blanding til opnåelse af sekvensresultater; og samling af sekvensresultaterne fra de separate blanding til komplette eller partielle nukleotidsekvenser for det ene eller de flere målpolynukleotider til frembringelse af contigs, hvor en sådan samling afhænger af udgangsblandingen for mindst en del af sekvensinformationen.
2. Fremgangsmåde ifølge krav 1, hvor trinet frembringelse indbefatter replikering af hvert af fragmenterne med den første størrelse i de separate blandinger.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor hvert af sekvensresultaterne fra den ene eller de flere fragmenter med den første størrelse i hver separate blanding har en længde, der er mindre end længderne af fragmenterne med den første størrelse, og antallet af sekvensresultater vælges, således at mindst 30 % af fragmenterne med den første størrelse sekventeres.
4. Fremgangsmåde ifølge kravene 1 til 3, der yderligere indbefatter et trin med tagging af hvert af fragmenterne med den første størrelse med et oligonukleotidtag, således at oligonukleotidtagget identificerer udgangsblandingen for fragmentet med den første størrelse.
5. Fremgangsmåde ifølge krav 4, hvor trinet opnåelse af sekvensresultater fra et eller flere fragmenter med den første størrelse i hver separate blanding indbefatter bestemmelse af nukleotidsekvensen for oligonukleotidtagget, og hvor eventuelt opnåelsen af sekvensresultater omfatter sekventering med højt gennemløb.
6. Fremgangsmåde ifølge krav 3, hvor trinet opnåelse af sekvensresultater indbefatter: generering for hver af de separate blandinger af en flerhed af målconcatemerer fra fragmenterne med den første størrelse, idet hver målconcatemer omfatter flere kopier af en del af et fragment med en første størrelse, og hver flerhed af målconcatemerer indbefatter et antal af sådanne dele, der dækker fragmentet med den første størrelse; frembringelse for hver af de separate blandinger af et tilfældigt array af målconcatemerer, der er fikseret til en overflade ved en densitet, således at mindst størstedelen af målconcatemererne kan opløses optisk; og opnåelse af sekvensresultater fra målconcatemerer for hver af de separate blandinger, hvor sådanne sekvensresultater hver har en længde, der er mindre end længderne af fragmenterne med den første størrelse, og hver af sådanne antal af sekvensresultater er valgt, således at sekvensresultaterne for hver separate blanding dækker fragmenterne med den første størrelse deri.
7. Fremgangsmåde ifølge krav 6, hvor trinet opnåelse af sekvensresultater omfatter: (a) hybridisering af en eller flere prober fra et første sæt af prober til hvert tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (b) hybridisering af en eller flere prober fra et andet sæt af prober til det tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (c) ligering af prober fra det første og andet sæt, der er hybridiseret til en målconcatemer på fortløbende steder; (d) identificering af sekvenserne af det ligerede første og andet sæt af prober; og (e) gentagelse af trinene (a) til (d) for hvert tilfældige array, indtil sekvenserne af fragmenterne med den første størrelse i hver af de separate blandinger er bestemt ud fra identiteterne af sekvenserne af de ligerede prober.
8. Fremgangsmåde ifølge kravene 1 til 7, hvor det ene eller de flere målpolynukleotider indeholder repetitive sekvensregioner, der hver har en længde, der er større end længderne af sekvensresultaterne.
9. Fremgangsmåde ifølge krav 2, hvor gennemsnitslængden af fragmenterne med den første størrelse ligger i intervallet fra 50 til 2000 baser.
10. Fremgangsmåde ifølge kravene 1 til 9, hvor gennemsnitslængden af fragmenterne med den første størrelse ligger i intervallet fra en tredjedel til en tusindedel af længden af det ene eller de flere målpolynukleotider.
11. Fremgangsmåde ifølge krav 1, der yderligere omfatter: tilfældig fragmentering af hver af fragmenterne med den første størrelse i hver af blandingerne til frembringelse af en population af fragmenter med en anden størrelse for hver blanding, således at fragmenterne med en anden størrelse har en gennemsnitslængde, der er mindre end længderne af fragmenterne med den første størrelse, og således at udgangsblandingen for hver af fragmenterne med en anden størrelse er bestemmelig.
12. Fremgangsmåde ifølge krav 1, hvor samlingen omfatter: samling af sekvensresultater til rekonstruktion af sekvensen af fragmenter med hver separate blanding; og samling af overlappende fragmenter fra uafhængige separate blandinger til frembringelse af de komplette eller partielle nukleotidsekvenser for målpolynukleotidet eller målpolynukleotiderne
13. Fremgangsmåde ifølge krav 11, hvor fragmenterne med den anden størrelse i de separate blandinger tagges til indikering af udgangsportionen, og samlingen omfatter: gruppering af sekvensresultaterne ved hjælp af tagsekvensen, og samling af sekvens-contigs derfra; samling af overlappende sekvens-contigs fra uafhængige portioner til frembringelse af komplette eller partielle nukleotidsekvenser for målpolynukleotidet eller målpolynukleotiderne.
14. Fremgangsmåde ifølge krav 1, hvor samlingen omfatter: samling af sekvensinformation i hver portion til rekonstruktion af sekvenserne af fragmenterne; samling af sekvenser af fragmenterne til rekonstruktion af sekvensen af målpolynukleotidet i en prøve.
15. Fremgangsmåde ifølge krav 11, hvor gennemsnitslængden af fragmenterne med den første størrelse er mindre end 300 kilobaser, og gennemsnitslængden af fragmenterne med den anden størrelse ligger i intervallet fra 50 til 600 baser.
16. Fremgangsmåde ifølge krav 11, hvor den forudbestemte dækningsmængde ligger i intervallet fra 3 til 30.
17. Fremgangsmåde ifølge kravene 1 til 16, hvor det ene eller de flere målpolynukleotider er et eller flere bakterielle genomer.
18. Fremgangsmåde ifølge kravene 11 til 16, hvor det ene eller de flere målpolynukleotider er haploide strenge af et mammaliagenom.
19. Fremgangsmåde ifølge krav 4, der yderligere omfatter puljning af de taggede fragmenter med den første størrelse i en enkelt blanding før opnåelse af sekvensresultaterne.
20. Fremgangsmåde ifølge krav 19, som er en fremgangsmåde til sekventering med højt gennemløb.
21. Fremgangsmåde ifølge kravene 1 til 20, der yderligere indbefatter separering af fragmenterne med den første størrelse efter størrelse før trinet frembringelse af de separate blandinger.
22. Fremgangsmåde ifølge krav 11, hvor trinet sekventering omfatter trinene: generering for hver af de separate blandinger af en flerhed af målconcatemerer fra fragmenterne med den anden størrelse, idet hver målconcatemer omfatter flere kopier af et fragment med den anden størrelse, og hver flerhed af målconcatemerer indbefatter et antal fragmenter med den anden størrelse, der dækker fragmenterne med den første størrelse i dets respektive separate blanding; frembringelse for hver af de separate blandinger af et tilfældigt array af målconcatemerer, der er fikseret til en overflade ved en densitet, således at mindst størstedelen af målconcatemererne kan opløses optisk; og generering af et antal sekvensresultater fra målconcatemerer i hver af de separate blandinger, idet sådanne sekvensresultater hver har en længde, der er mindre end længderne af fragmenterne med den anden størrelse, og hvert af sådanne antal af sekvensresultater er valgt, således at sekvensresultaterne af hver separate blanding dækker fragmenterne med den anden størrelse deri.
23. Fremgangsmåde ifølge krav 22, hvor trinet opnåelse af sekvensresultaterne omfatter: (a) hybridisering af en eller flere prober fra et første sæt af prober til hvert tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (b) hybridisering af en eller flere prober fra et andet sæt af prober til det tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (c) ligering af prober fra det første og andet sæt, der er hybridiseret til en målconcatemer på fortløbende steder; (d) identificering af sekvenserne af det ligerede første og andet sæt af prober; og (e) gentagelse af trinene (a) til (d) for hvert tilfældige array, indtil sekvenserne af fragmenterne med den anden størrelse i hver af de separate blandinger er bestemt ud fra identiteterne af sekvenserne af de ligerede prober.
24. Fremgangsmåde ifølge krav 22 eller 23, hvor hver af fragmenterne med den første størrelse har et oligonukleotidtag bundet til en ende, hvilket oligonukleotidtag identificerer den separate blanding af et sådant fragment med en første størrelse.
25. Fremgangsmåde til sekventering af nukleotidsekvenser af et eller flere målpolynukleotider, hvilken fremgangsmåde omfatter trinene: frembringelse af flere niveauer af blandinger, der omfatter et hierarki af indlejrede fragmenter af det ene eller de flere målpolynukleotider fremstillet ved tilfældig fragmentering, hvor hver blanding af hvert forudgående niveau opdeles i et antal blandinger på et efterfølgende niveau, idet mindst ét niveau har blandinger med ikke-overlappende fragmenter, og det ene eller de flere niveauer har et sidste niveau, hvor blandinger af forudgående niveauer kan identificeres for hvert fragment i hver blanding på det sidste niveau; opnåelse af sekvensresultater fra mindst en del af et eller flere fragmenter i hver blanding på det sidste niveau; og tilvejebringelse af komplette eller partielle nukleotidsekvenser af det ene eller de flere målpolynukleotider ved hjælp af samling af sekvensresultaterne fra det sidste niveau af blandinger til frembringelse af contigs, hvor en sådan samling afhænger af udgangsblandingen på mindst ét af niveauerne.
26. Fremgangsmåde ifølge krav 25, hvor trinet frembringelse indbefatter replikering af fragmenterne i hver af blandingerne på hvert af de efterfølgende niveauer.
27. Fremgangsmåde ifølge krav 25 eller 26, hvor samlingen af sekvensresultaterne afhænger af identiteterne af blandingerne på hvert af det ene eller de flere niveauer.
28. Fremgangsmåde ifølge kravs 25 til 27, der yderligere indbefatter et trin med tagging af hvert af fragmenterne på hvert af niveauerne med et oligonukleotidtag, således at oligonukleotidtagget identificerer udgangsblandingen for hvert af fragmenterne på hvert niveau; og hvor blandingerne forud for trinet opnåelse af sekvensresultater fra det sidste niveau puljes sammen til en enkelt blanding.
29. Fremgangsmåde ifølge krav 28, hvor trinet bestemmelse af nukleotidsekvensen af delen af fragmenterne på den sidste niveau indbefatter sekventering af hvert af oligonukleotidtaggene.
30. Fremgangsmåde ifølge kravene 25 til 29, hvor det ene eller de flere målpolynukleotider indeholder repetitive sekvensregioner, der hver har en længde, der er større end længden af sekvensresultaterne.
31. Fremgangsmåde ifølge kravene 1 til 24, hvor trinet tilfældig fragmentering udføres ved hjælp af en fremgangsmåde udvalgt blandt sonikering, passage gennem kapillarer, dispersion af en DNA-opløsning i fine dråber, behandling med DNase I, behandling med endonuclease og tagget PCR-amplifikation .
32. Fremgangsmåde ifølge kravene 1 til 31, der tilvejebringer haplotype-information for et diploidt genom.
33. Fremgangsmåde ifølge krav 25, hvor der dannes en flerhed af niveauer af blandinger ved hjælp af en proces, der omfatter trinene: (a) tilfældig fragmentering af det ene eller de flere målpolynukleotider, der er til sted, i en forudbestemt dækningsmængde til frembringelse af en population, der indeholder overlappende fragmenter med en første størrelse, som hver har en gennemsnitslængde, der er mindre end længderne af målpolynukleotiderne, idet fragmenterne med den første størrelse er de første fragmenter med forudgående størrelse; (b) generering af et sæt af separate blandinger fra hver population af fragmenter med forudgående størrelse, idet et sådant sæt har et niveau og et antal blandinger deri, hvor hvert sådant antal er valgt, således at mindst 60 % af de separate blandinger kun indeholder ikke-overlappende fragmenter; (c) tilfældig fragmentering af hvert af fragmenterne med den forudgående størrelse i hver af blandingerne til frembringelse af en population af fragmenter med en anden størrelse for hver blanding, således at hvert fragment med den anden størrelse har en gennemsnitslængde, der er mindre end gennemsnitslængderne af fragmenterne med den første størrelse, og således at udgangsblandingerne for hvert fragment med den anden størrelse kan identificeres, idet fragmenterne med den anden størrelse er fragmenterne med den forudgående størrelse for et næste fragmenteringstrin; (d) gentagelse af trinene (b) og (c) efter behov, indtil det sidste niveau af blandinger er opnået; og hvor sekvensresultaterne opnås fra mindst en del af et eller flere fragmenter med den anden størrelse i hver blanding på det sidste niveau.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11414702B2 (en) 2005-06-15 2022-08-16 Complete Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments

Families Citing this family (700)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
DE60142709D1 (de) 2000-12-13 2010-09-09 Nugen Technologies Inc Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
IL153504A0 (en) 2001-03-09 2003-07-06 Nugen Technologies Inc Methods and compositions for amplification of rna sequences
WO2004076683A2 (en) * 2003-02-26 2004-09-10 Callida Genomics, Inc. Random array dna analysis by hybridization
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
US8785353B2 (en) 2005-06-23 2014-07-22 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
DK1929039T4 (en) 2005-09-29 2014-02-17 Keygene Nv High throughput-screening af mutageniserede populationer
US10316364B2 (en) * 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
CA2624896C (en) * 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
EP1966393B1 (en) 2005-12-22 2012-07-25 Keygene N.V. Method for high-throughput aflp-based polymorphism detection
EP1994180A4 (en) * 2006-02-24 2009-11-25 Callida Genomics Inc GENOME SEQUENCING ON DNA ARRAYS WITH HIGH THROUGHPUT
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN1876840A (zh) * 2006-04-26 2006-12-13 东南大学 一种多拷贝的单分子核酸阵列芯片
US7910354B2 (en) * 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US7629125B2 (en) * 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US9874501B2 (en) 2006-11-24 2018-01-23 Curiox Biosystems Pte Ltd. Use of chemically patterned substrate for liquid handling, chemical and biological reactions
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2653861B1 (en) 2006-12-14 2014-08-13 Life Technologies Corporation Method for sequencing a nucleic acid using large-scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US12497656B2 (en) 2007-01-26 2025-12-16 Illumina, Inc. Independently removable nucleic acid sequencing system and method
EP2108042A4 (en) * 2007-02-02 2010-04-14 California Inst Of Techn SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
WO2008121384A1 (en) * 2007-03-30 2008-10-09 University Of Georgia Research Foundation, Inc. Source tagging and normalization of dna for parallel dna sequencing, and direct measurement of mutation rates using the same
CA2689356A1 (en) * 2007-06-01 2008-12-11 454 Life Sciences Corporation System and meth0d for identification of individual samples from a multiplex mixture
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
DE102007034967A1 (de) 2007-07-26 2009-01-29 Plansee Se Brennstoffzelle und Verfahren zu deren Herstellung
US8759077B2 (en) * 2007-08-28 2014-06-24 Lightspeed Genomics, Inc. Apparatus for selective excitation of microparticles
US8222040B2 (en) * 2007-08-28 2012-07-17 Lightspeed Genomics, Inc. Nucleic acid sequencing by selective excitation of microparticles
US20090061424A1 (en) * 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
ITBO20070627A1 (it) * 2007-09-14 2009-03-15 Twof Inc Metodo per la preparazione di dna microarray con sonde ad alta densita' lineare
US8003330B2 (en) 2007-09-28 2011-08-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
US8951731B2 (en) * 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US7901890B2 (en) * 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8415099B2 (en) * 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
DK2215209T3 (da) 2007-10-30 2018-09-03 Complete Genomics Inc Apparat til high-throughput-sekventering af nukleinsyrer
US7988918B2 (en) 2007-11-01 2011-08-02 Complete Genomics, Inc. Structures for enhanced detection of fluorescence
WO2013114217A1 (en) 2012-02-05 2013-08-08 Curiox Biosystems Pte Ltd. Array plates and methods for making and using same
US10725020B2 (en) 2007-11-14 2020-07-28 Curiox Biosystems Pte Ltd. High throughput miniaturized assay system and methods
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
DK2565279T3 (da) 2007-12-05 2015-02-16 Complete Genomics Inc Effektiv basebestemmelse i sekvenseringsreaktioner
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
EP2227563B1 (en) * 2007-12-05 2012-06-06 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US20090171640A1 (en) * 2007-12-28 2009-07-02 Microsoft Corporation Population sequencing using short read technologies
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US20090270273A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP5774474B2 (ja) * 2008-05-02 2015-09-09 エピセンター テクノロジーズ コーポレーションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ Ｒｎａへの選択的な５’ライゲーションによるタグの付加
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
TWI460602B (zh) * 2008-05-16 2014-11-11 Counsyl Inc 廣用的懷孕前篩檢裝置
EP2307577B1 (en) 2008-06-25 2015-06-03 Life Technologies Corporation Methods for measuring analytes using large scale fet arrays
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
WO2010028140A2 (en) 2008-09-03 2010-03-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP3272879B1 (en) 2008-10-24 2019-08-07 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
WO2010053587A2 (en) 2008-11-07 2010-05-14 Mlc Dx Incorporated Methods of monitoring conditions by sequence analysis
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
PL2387627T3 (pl) 2009-01-15 2016-10-31 Profilowanie odporności adaptacyjnej i wytwarzanie przeciwciał monoklonalnych
WO2010091024A1 (en) * 2009-02-03 2010-08-12 Complete Genomics, Inc. Oligomer sequences mapping
WO2010091023A2 (en) * 2009-02-03 2010-08-12 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
EP2394164A4 (en) * 2009-02-03 2014-01-08 Complete Genomics Inc ASSIGNMENT OF OLIGOMER SEQUENCES
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
CN102460155B (zh) 2009-04-29 2015-03-25 考利达基因组股份有限公司 用于关于参考多核苷酸序列标注样本多核苷酸序列中的变异的方法和系统
US20130296189A1 (en) * 2009-05-08 2013-11-07 Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences Probes utilizing universal tags, a kit comprising the same and detection methods
US8246799B2 (en) 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US9524369B2 (en) * 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
EP2443236B1 (en) 2009-06-15 2015-05-13 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
KR20120044941A (ko) 2009-06-25 2012-05-08 프레드 헛친슨 켄서 리서치 센터 적응 면역의 측정방법
KR20110018763A (ko) * 2009-08-18 2011-02-24 삼성전자주식회사 표적 물질을 기판에 고정하는 방법 및 장치
WO2011021102A2 (en) * 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US10385391B2 (en) * 2009-09-22 2019-08-20 President And Fellows Of Harvard College Entangled mate sequencing
US20120185176A1 (en) 2009-09-30 2012-07-19 Natera, Inc. Methods for Non-Invasive Prenatal Ploidy Calling
CA2779606A1 (en) * 2009-11-10 2011-05-19 Nestec S.A. Heart aging biomarkers and methods of use
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP4378584A3 (en) 2010-02-19 2024-11-13 Pacific Biosciences Of California, Inc. Integrated analytical system and method for fluorescence measurement
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
US9465228B2 (en) 2010-03-19 2016-10-11 Optical Biosystems, Inc. Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns
US8502867B2 (en) 2010-03-19 2013-08-06 Lightspeed Genomics, Inc. Synthetic aperture optics imaging method using minimum selective excitation patterns
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
ES2555106T3 (es) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Ensayos biológicos codificados espacialmente
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US8774494B2 (en) * 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
CA3037126C (en) 2010-05-18 2023-09-12 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
AU2011226792A1 (en) 2010-06-11 2012-01-12 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
TWI569025B (zh) 2010-06-30 2017-02-01 生命技術公司 用於測試離子感測場效電晶體（ｉｓｆｅｔ）陣列之裝置及方法
CN119165030A (zh) 2010-06-30 2024-12-20 生命科技公司 阵列列积分器
TWI624665B (zh) 2010-06-30 2018-05-21 生命技術公司 離子感測電荷累積電路及方法
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (zh) 2010-07-03 2016-03-21 生命技術公司 具有微摻雜汲極之化學感測器
KR101862756B1 (ko) * 2010-07-09 2018-05-30 세르겐티스 비.브이. 관심있는 3-d 게놈 영역 서열화 전략
WO2012011877A2 (en) 2010-07-23 2012-01-26 Curiox Biosystems Pte Ltd Apparatus and method for multiple reactions in small volumes
AU2011291538A1 (en) * 2010-08-20 2013-03-14 The Regents Of The University Of California Small molecule arrays and methods for making and using them
US9671344B2 (en) * 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
FR2964391B1 (fr) * 2010-09-03 2014-04-11 Centre Nat Rech Scient Biopuces pour l'analyse de la dynamique de molecules d'acide nucleique
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
AU2011226766A1 (en) 2010-09-24 2012-04-12 Life Technologies Corporation Matched pair transistor circuits
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
WO2012058096A1 (en) 2010-10-27 2012-05-03 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2640849B1 (en) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
BR112013016193B1 (pt) 2010-12-22 2019-10-22 Natera, Inc. método ex vivo para determinar se um suposto pai é o pai biológico de um feto que está em gestação em uma gestante e relatório
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2658999B1 (en) 2010-12-30 2019-03-13 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
BR112013020220B1 (pt) 2011-02-09 2020-03-17 Natera, Inc. Método para determinar o estado de ploidia de um cromossomo em um feto em gestação
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
CN105861645B (zh) 2011-04-08 2020-02-21 生命科技股份有限公司 用于合成测序中的相保护试剂流排序
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN107368705B (zh) * 2011-04-14 2021-07-13 完整基因有限公司 分析生物体的基因组dna的方法和计算机系统
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
EP4324935B1 (en) 2011-08-18 2025-02-19 Life Technologies Corporation Methods, systems and computer readable media for making base calls in nucleic acid sequencing
US9679103B2 (en) 2011-08-25 2017-06-13 Complete Genomics, Inc. Phasing of heterozygous loci to determine genomic haplotypes
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
AU2012304328B2 (en) * 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
EP3981886A1 (en) 2011-09-23 2022-04-13 Illumina, Inc. Compositions for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP2771103B1 (en) 2011-10-28 2017-08-16 Illumina, Inc. Microarray fabrication system and method
US9708649B2 (en) 2011-10-31 2017-07-18 Hitachi High-Technologies Corporation Method and substrate for nucleic acid amplification, and method and apparatus for nucleic acid analysis
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
EP3904536B1 (en) 2011-12-09 2025-10-29 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP2605001A1 (en) * 2011-12-15 2013-06-19 Hain Lifescience GmbH A device and method for optically measuring fluorescence of nucleic acids in test samples and use of the device and method
US9404147B2 (en) * 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP4108782B2 (en) 2011-12-22 2026-01-14 President and Fellows of Harvard College Compositions and methods for analyte detection
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
EP2812344A4 (en) 2012-02-07 2015-10-28 Vibrant Holdings Llc SUBSTRATES, PEPTIDE NETWORKS AND METHODS
US20130217023A1 (en) * 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
CA2866625C (en) * 2012-03-13 2020-12-08 Swift Biosciences, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
WO2013138767A1 (en) 2012-03-16 2013-09-19 Life Technologies Corporation Systems and methods for biological analysis
US9803239B2 (en) * 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
ES2949570T3 (es) 2012-04-03 2023-09-29 Illumina Inc Cabezal integrado de lectura optoelectrónica y cartucho de fluidos útiles para la secuenciación de ácidos nucleicos
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
HK1203220A1 (en) 2012-05-04 2015-10-23 考利达基因组股份有限公司 Methods for determining absolute genome-wide copy number variations of complex tumors
KR101621643B1 (ko) 2012-05-08 2016-05-16 어댑티브 바이오테크놀로지스 코포레이션 다중 pcr 반응에서 증폭 바이어스를 측정 및 교정하기 위한 조성물 및 방법
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US9052963B2 (en) 2012-05-21 2015-06-09 International Business Machines Corporation Cloud computing data center machine monitor and control
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014008635A1 (zh) * 2012-07-11 2014-01-16 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10895534B2 (en) 2012-08-20 2021-01-19 Illumina, Inc. Method and system for fluorescence lifetime based sequencing
EP3974564A1 (en) * 2012-09-28 2022-03-30 Vibrant Holdings, LLC Methods, systems, and arrays for biomolecular analysis
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
EP3640343B1 (en) 2012-10-01 2026-01-07 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2014071322A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Small RNA Capture, Detection and Quantification
US10286376B2 (en) 2012-11-14 2019-05-14 Vibrant Holdings, Llc Substrates, systems, and methods for array synthesis and biomolecular analysis
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
EP4012716A1 (en) 2012-11-19 2022-06-15 Apton Biosystems, Inc. Digital analysis of molecular analytes using single molecule detection
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013359165B2 (en) * 2012-12-14 2019-09-12 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014113204A1 (en) 2013-01-17 2014-07-24 Personalis, Inc. Methods and systems for genetic analysis
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
WO2014121091A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US20150369772A1 (en) * 2013-02-07 2015-12-24 Koninklijke Philips N.V. Processing of nucleotide sequences
CA2900543C (en) 2013-02-08 2023-01-31 10X Genomics, Inc. Partitioning and processing of analytes and other species
EP4030167B1 (en) 2013-02-15 2025-05-14 Vibrant Holdings, LLC Methods and compositions for amplified electrochemiluminescence detection
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
ES2887177T3 (es) 2013-03-13 2021-12-22 Illumina Inc Método de preparación de una biblioteca de secuenciación de ácidos nucleicos
PL2969479T3 (pl) 2013-03-13 2021-12-27 Illumina, Inc. Wielowarstwowe urządzenia płynowe i sposoby ich wytwarzania
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
EP2971071B1 (en) 2013-03-15 2018-02-28 Illumina, Inc. Enzyme-linked nucleotides
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
CA2906218A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US20140264471A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical device with thin conductive element
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
JP6581074B2 (ja) 2013-03-15 2019-09-25 ライフ テクノロジーズ コーポレーション 一貫性のあるセンサ表面積を有する化学センサ
WO2014151511A2 (en) * 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
CN113846096B (zh) 2013-06-04 2025-05-09 哈佛大学校长及研究员协会 Rna-导向的转录调控
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
CN105473744B (zh) 2013-06-25 2020-06-19 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
TR201816438T4 (tr) 2013-07-01 2018-11-21 Illumina Inc Katalizörsüz yüzey işlevselleştirme ve polimer aşılama.
CA2914248C (en) 2013-07-03 2021-09-07 Illumina, Inc. Sequencing by orthogonal synthesis
US9557318B2 (en) 2013-07-09 2017-01-31 Curiox Biosystems Pte Ltd. Array plates for washing samples
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
TWI805996B (zh) 2013-08-05 2023-06-21 美商扭轉生物科技有限公司 重新合成之基因庫
KR102043320B1 (ko) 2013-08-08 2019-11-11 일루미나, 인코포레이티드 플로우 셀로의 시약 전달을 위한 유체 시스템
US20160201119A1 (en) 2013-08-22 2016-07-14 Apton Biosystems, Inc. Digital Analysis of Molecular Analytes Using Electrical Methods
CN105637099B (zh) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 使用短读段的长片段从头组装
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CA2920390A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
WO2015051338A1 (en) 2013-10-04 2015-04-09 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
EP3058092B1 (en) 2013-10-17 2019-05-22 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2015088913A1 (en) 2013-12-09 2015-06-18 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
NZ720871A (en) 2013-12-10 2020-03-27 Illumina Inc Biosensors for biological or chemical analysis and methods of manufacturing the same
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3083994B1 (en) 2013-12-20 2021-08-18 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
JP2015139373A (ja) * 2014-01-27 2015-08-03 株式会社日立ハイテクノロジーズ 生体分子分析デバイス、及び生体分子分析装置
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
EP3122894A4 (en) * 2014-03-28 2017-11-08 GE Healthcare Bio-Sciences Corp. Accurate detection of rare genetic variants in next generation sequencing
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
DE202015009609U1 (de) 2014-04-10 2018-08-06 10X Genomics, Inc. Mikrofluidisches System zur Erzeugung von Emulsionen
CA2945962C (en) 2014-04-21 2023-08-29 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
WO2015168541A1 (en) * 2014-05-02 2015-11-05 Synthetic Genomics, Inc. Tamper-resistant assembly for securing valuable material
SG10201809312QA (en) 2014-05-16 2018-11-29 Illumina Inc Nucleic acid synthesis techniques
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US10829814B2 (en) 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
AU2015279546B2 (en) 2014-06-26 2021-04-08 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
SG11201700348VA (en) 2014-07-15 2017-02-27 Illumina Inc Biochemically activated electronic device
US20160032281A1 (en) * 2014-07-31 2016-02-04 Fei Company Functionalized grids for locating and imaging biological specimens and methods of using the same
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
WO2016031247A1 (ja) 2014-08-26 2016-03-03 国立研究開発法人科学技術振興機構 非酵素的核酸鎖結合方法
CN107002121B (zh) 2014-09-18 2020-11-13 亿明达股份有限公司 用于分析核酸测序数据的方法和系统
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
EP3212807B1 (en) 2014-10-29 2020-09-02 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
EP3715455A1 (en) 2014-10-29 2020-09-30 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
ES2905706T3 (es) 2014-10-31 2022-04-11 Illumina Cambridge Ltd Polímeros y recubrimientos de copolímeros de ADN
CN107250358B (zh) 2014-11-05 2021-03-30 伊卢米纳剑桥有限公司 在样品制备和测序期间使用铁载体螯合剂减少dna损伤
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
JP6805140B2 (ja) 2014-11-21 2020-12-23 リサーチ インスティチュート アット ネイションワイド チルドレンズ ホスピタル 平行プロセシングシステムおよび生物配列データの高度にスケーラブルな解析の方法
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
CN107124888B (zh) 2014-11-21 2021-08-06 深圳华大智造科技股份有限公司 鼓泡状接头元件和使用其构建测序文库的方法
WO2016086029A1 (en) 2014-11-25 2016-06-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
WO2016100196A1 (en) * 2014-12-15 2016-06-23 Illumina, Inc. Compositions and methods for single molecular placement on a substrate
TWI832669B (zh) 2014-12-18 2024-02-11 美商生命技術公司 具有傳輸器組態的高資料速率積體電路
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
TWI684004B (zh) 2014-12-18 2020-02-01 美商生命技術公司 用於使用大規模fet陣列量測分析物之方法及設備
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
US10908083B2 (en) * 2015-02-02 2021-02-02 Hitachi High-Tech Corporation Multicolor fluorescence analysis device
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
AU2016219480B2 (en) 2015-02-09 2021-11-11 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
NZ734854A (en) 2015-02-17 2022-11-25 Dovetail Genomics Llc Nucleic acid sequence assembly
EP3259372B1 (en) 2015-02-17 2020-11-18 MGI Tech Co., Ltd. Dna sequencing using controlled strand displacement
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
ES3002665T3 (en) 2015-02-24 2025-03-07 Adaptive Biotechnologies Corp Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
EP3268491B1 (en) 2015-03-13 2021-07-14 Life Technologies Corporation Methods, compositions and kits for small rna capture, detection and quantification
CN119779988A (zh) 2015-03-24 2025-04-08 伊鲁米那股份有限公司 对样品成像用于生物或化学分析的方法、载体组件和系统
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
ES2942572T3 (es) 2015-05-11 2023-06-02 Illumina Inc Plataforma para el descubrimiento y análisis de agentes terapéuticos
EP4220645A3 (en) 2015-05-14 2023-11-08 Life Technologies Corporation Barcode sequences, and related systems and methods
JP6657262B2 (ja) 2015-05-29 2020-03-04 イラミーナ インコーポレーテッド 指定された反応を行わせるための試料支持体及び分析システム
US10545139B2 (en) 2015-06-16 2020-01-28 Curiox Biosystems Pte Ltd. Methods and devices for performing biological assays using magnetic components
HK1253950A1 (zh) 2015-07-07 2019-07-05 Illumina, Inc. 经由纳米压印的选择性表面图案化
CN107835857A (zh) 2015-07-17 2018-03-23 亿明达股份有限公司 用于测序应用的聚合物片层
WO2017019278A1 (en) 2015-07-30 2017-02-02 Illumina, Inc. Orthogonal deblocking of nucleotides
EP3338096B1 (en) 2015-08-24 2024-03-06 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
HK1253910A1 (zh) 2015-09-02 2019-07-05 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
HK1259101A1 (zh) 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
CN108474022A (zh) 2015-11-03 2018-08-31 哈佛学院董事及会员团体 用于包含三维核酸的基质容积成像的设备和方法
US10253352B2 (en) 2015-11-17 2019-04-09 Omniome, Inc. Methods for determining sequence profiles
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
CN108474745A (zh) 2016-01-11 2018-08-31 伊鲁米那股份有限公司 具有显微荧光计、流体系统和流动池闩锁夹持模块的检测装置
US11081208B2 (en) 2016-02-11 2021-08-03 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
CA3014911A1 (en) 2016-02-23 2017-08-31 Dovetail Genomics, Llc Generation of phased read-sets for genome assembly and haplotype phasing
JP6584986B2 (ja) 2016-03-18 2019-10-02 株式会社東芝 核酸検出方法
ES2913468T3 (es) 2016-04-15 2022-06-02 Natera Inc Métodos para la detección del cáncer de pulmón.
CA3022290A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
DK3455356T3 (da) 2016-05-13 2021-11-01 Dovetail Genomics Llc Genfinding af langtrækkende bindingsinformation fra konserverede prøver
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
AU2017267653B2 (en) 2016-05-18 2021-05-13 Illumina, Inc. Self assembled patterning using patterned Hydrophobic surfaces
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
CN109643578B (zh) 2016-06-01 2023-07-21 生命科技股份有限公司 用于设计基因组合的方法和系统
WO2018015365A1 (en) * 2016-07-18 2018-01-25 Roche Sequencing Solutions, Inc. Asymmetric templates and asymmetric method of nucleic acid sequencing
CN109844133A (zh) * 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP3507385A4 (en) 2016-08-31 2020-04-29 President and Fellows of Harvard College METHOD FOR COMBINING THE DETECTION OF BIOMOLECULES IN A SINGLE TEST WITH FLUORESCENT IN SITU SEQUENCING
EP4582805A3 (en) 2016-08-31 2025-10-08 President And Fellows Of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
EP3516528B1 (en) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nucleic acid based data storage
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
EP3529400B1 (en) 2016-10-24 2021-02-17 Geneinfosec, Inc. Concealing information present within nucleic acids
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
WO2018085642A1 (en) 2016-11-03 2018-05-11 Complete Genomics, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
WO2018093780A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
WO2018102759A1 (en) 2016-12-01 2018-06-07 Ignite Biosciences, Inc. Methods of assaying proteins
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
WO2018112426A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3048486A1 (en) 2017-01-04 2018-07-12 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN110382710B (zh) 2017-01-24 2024-10-01 瓦斯特根公司 构建核酸分子拷贝的方法
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
KR20240158370A (ko) 2017-02-22 2024-11-04 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
JP7305611B2 (ja) * 2017-03-17 2023-07-10 アプトン バイオシステムズ インコーポレイテッド 配列決定および高分解能画像化の方法
CN118099095A (zh) 2017-03-20 2024-05-28 深圳华大智造科技股份有限公司 用于生物或化学分析的生物传感器及其制造方法
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
KR20220132668A (ko) 2017-04-05 2022-09-30 큐리옥스 바이오시스템즈 피티이 엘티디. 어레이 플레이트 상의 샘플을 세정하기 위한 방법, 디바이스 및 장치
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CN110870018B (zh) 2017-05-19 2024-11-22 10X基因组学有限公司 用于分析数据集的系统和方法
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP6991504B2 (ja) * 2017-07-13 2022-01-12 国立研究開発法人産業技術総合研究所 目的物質検出装置及び目的物質検出方法
CN110892057B (zh) 2017-08-01 2024-07-12 深圳华大智造科技股份有限公司 基因测序反应设备、基因测序系统和基因测序反应方法
BR112020003229A8 (pt) 2017-08-15 2023-05-09 Omniome Llc Aparelho e métodos de varredura úteis para detecção de analitos químicos e biológicos
EP3669018A4 (en) 2017-08-18 2021-05-26 Nautilus Biotechnology, Inc. Binding reagent selection methods
US11125748B2 (en) 2017-09-01 2021-09-21 University Of British Columbia Method for organizing individual molecules on a patterned substrate and structures assembled thereby
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111295733B (zh) 2017-09-19 2024-01-05 深圳华大智造科技股份有限公司 晶片级测序流通池制造
EP4421183A3 (en) 2017-09-22 2024-11-27 University of Washington In situ combinatorial labeling of cellular molecules
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US11485966B2 (en) 2017-10-11 2022-11-01 Mgi Tech Co., Ltd. Method for improving loading and stability of nucleic acid
JP6834029B2 (ja) 2017-10-16 2021-02-24 イルミナ インコーポレイテッド 深層畳み込みニューラルネットワークを訓練するための深層学習ベースの技法
EP3628099B1 (en) 2017-10-16 2024-05-15 Illumina, Inc. Deep learning-based splice site classification
AU2018353136B2 (en) 2017-10-19 2022-05-19 Pacific Biosciences Of California, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019118355A1 (en) 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
AU2018395430B2 (en) 2017-12-29 2024-05-23 Nautilus Subsidiary, Inc. Decoding approaches for protein identification
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
EP3735459B1 (en) 2018-01-04 2026-03-04 Atlas Data Storage, Inc. Dna-based digital information storage
KR102494589B1 (ko) 2018-01-08 2023-02-07 일루미나, 인코포레이티드 반도체-기반 검출을 사용한 고-처리율 서열분석
WO2019136388A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
CA3065939A1 (en) 2018-01-15 2019-07-18 Illumina, Inc. Deep learning-based variant classifier
WO2019152539A1 (en) 2018-01-30 2019-08-08 Optical Biosystems, Inc. Method for detecting particles using structured illumination
US12378592B2 (en) 2018-01-31 2025-08-05 Dovetail Genomics, Llc. Sample prep for DNA linkage recovery
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
JP7348603B2 (ja) * 2018-04-02 2023-09-21 エニュメラ・モレキュラー・インコーポレイテッド 核酸分子を計数するための方法、システム、および組成物
US11471884B2 (en) 2018-04-02 2022-10-18 Dropworks, Inc. Systems and methods for serial flow emulsion processes
JP7438966B2 (ja) * 2018-04-04 2024-02-27 ノーティラス・サブシディアリー・インコーポレイテッド ナノアレイおよびマイクロアレイを製造する方法
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
EP3781714B1 (en) 2018-04-14 2026-01-07 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
CN108444966A (zh) * 2018-04-16 2018-08-24 成都博奥晶芯生物科技有限公司 一种激光微阵列芯片扫描仪的浓度梯度荧光校准片
AU2019255987A1 (en) 2018-04-19 2020-12-10 Pacific Biosciences Of California, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP7554117B2 (ja) 2018-04-26 2024-09-19 パシフィック・バイオサイエンシズ・オブ・カリフォルニア・インク． 核酸－ヌクレオチド－ポリメラーゼ複合体を安定化するための方法及び組成物
WO2019217452A1 (en) 2018-05-08 2019-11-14 Mgi Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly
AU2019266303B2 (en) 2018-05-09 2025-12-18 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photactivated agents
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
CN111201329B (zh) 2018-05-17 2025-02-11 伊鲁米纳公司 具有减少的扩增偏倚的高通量单细胞测序
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2019231568A1 (en) 2018-05-31 2019-12-05 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
MX2019015262A (es) 2018-06-04 2023-01-25 Illumina Inc Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
JP2020000197A (ja) * 2018-06-29 2020-01-09 キヤノン株式会社 計数方法、濃度測定装置及び濃度測定システム
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
CA3107165A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
EP3830289B1 (en) 2018-08-03 2026-02-11 10X Genomics, Inc. Methods to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
KR20260023102A (ko) * 2018-09-19 2026-02-20 퍼시픽 바이오사이언스 오브 캘리포니아, 인크. 밀집 패킹된 분석물층 및 검출 방법
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
KR102165734B1 (ko) 2018-10-15 2020-10-14 일루미나, 인코포레이티드 심층 컨볼루션 신경망을 사전 훈련시키기 위한 심층 학습 기반 기술
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US20200149095A1 (en) * 2018-11-14 2020-05-14 Element Biosciences, Inc. Low binding supports for improved solid-phase dna hybridization and amplification
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
GB2598656B (en) * 2018-11-15 2023-10-04 Element Biosciences Inc Methods for generating circular nucleic acid molecules
TWI853851B (zh) 2018-11-15 2024-09-01 中國商深圳華大智造科技有限公司 微流體裝置與其製備方法
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
US11993865B2 (en) 2018-11-20 2024-05-28 Nautilus Subsidiary, Inc. Selection of affinity reagents
WO2020108588A1 (en) 2018-12-01 2020-06-04 Mgi Tech Co., Ltd. Methods and structures to improve light collection efficiency in biosensors
GB2578528B (en) 2018-12-04 2021-02-24 Omniome Inc Mixed-phase fluids for nucleic acid sequencing and other analytical assays
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894592A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating spatial arrays with gradients
CN112654715B (zh) 2018-12-19 2024-12-31 亿明达股份有限公司 改进多核苷酸簇克隆性优先性的方法
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
EP3918088B1 (en) 2019-01-29 2024-03-13 MGI Tech Co., Ltd. High coverage stlfr
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
EP3930753A4 (en) 2019-02-26 2023-03-29 Twist Bioscience Corporation VARIANT NUCLEIC ACID LIBRARIES FOR GLP1 RECEPTOR
EP3837365A1 (en) 2019-03-01 2021-06-23 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
WO2020180813A1 (en) 2019-03-06 2020-09-10 Qiagen Sciences, Llc Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020205296A1 (en) 2019-03-21 2020-10-08 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
SG11202102801TA (en) 2019-04-29 2021-04-29 Illumina Inc Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
CA3145806A1 (en) * 2019-05-13 2020-11-19 Rapid Genomics Llc Capture and analysis of target genomic regions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
AU2020276115A1 (en) 2019-05-16 2021-01-07 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11841374B2 (en) * 2019-06-27 2023-12-12 Mjnn Llc Laboratory automation system implementing efficient path for material and lab ware transfers
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
US20230220466A1 (en) * 2019-09-10 2023-07-13 The Regents Of The University Of California Immune cell sequencing methods
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
EP4464827A3 (en) 2019-09-23 2025-02-26 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
EP4045683B1 (en) 2019-10-18 2025-02-19 Pacific Biosciences of California, Inc. Methods for capping nucleic acids
JP7470787B2 (ja) 2019-11-05 2024-04-18 パーソナリス，インコーポレイティド 単一試料からの腫瘍純度の推定
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
US12570750B2 (en) 2019-12-09 2026-03-10 Twist Bioscience Corporation Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
CN115516367B (zh) 2019-12-10 2025-11-11 康泰博实验室公司 用于三维光片成像的方法和系统
MX2021011847A (es) 2019-12-19 2021-11-17 Illumina Inc Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas.
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
US12448648B2 (en) 2019-12-23 2025-10-21 Mgi Tech Co., Ltd. Controlled strand-displacement for paired end sequencing
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021163630A1 (en) 2020-02-13 2021-08-19 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
EP4693300A2 (en) 2020-02-20 2026-02-11 Illumina, Inc. Artificial intelligence-based many-to-many base calling
IL295587A (en) 2020-02-20 2022-10-01 Illumina Inc A bus network for a base reader based on artificial intelligence
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
WO2021178467A1 (en) 2020-03-03 2021-09-10 Omniome, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021188717A1 (en) * 2020-03-18 2021-09-23 Apton Biosystems, Inc. Systems and methods of detecting densely-packed analytes
TWI803855B (zh) * 2020-04-21 2023-06-01 美商西方數位科技公司 用於定序核酸之系統及裝置、定序複數個ｓ個核酸股之方法及減輕由於使用單分子感測器陣列之核酸定序程序而產生之定序資料之錯誤之方法
EP4549582A3 (en) 2020-04-22 2025-06-18 10x Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153798A4 (en) * 2020-05-20 2024-05-01 Element Biosciences, Inc. Methods for paired-end sequencing library preparation
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
CN116249785A (zh) 2020-06-02 2023-06-09 10X基因组学有限公司 用于抗原-受体的空间转录组学
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4162075A4 (en) 2020-06-08 2024-06-26 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4164796A4 (en) 2020-06-10 2024-03-06 10x Genomics, Inc. Fluid delivery methods
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
GB202010691D0 (en) * 2020-07-10 2020-08-26 Vidya Holdings Ltd Improvements in or relating to an apparatus for imaging
WO2022015913A1 (en) * 2020-07-17 2022-01-20 The Regents Of The University Of Michigan Materials and methods for localized detection of nucleic acids in a tissue sample
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12469162B2 (en) 2020-08-31 2025-11-11 Element Biosciences, Inc. Primary analysis in next generation sequencing
US11200446B1 (en) 2020-08-31 2021-12-14 Element Biosciences, Inc. Single-pass primary analysis
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
US12505571B2 (en) 2020-08-31 2025-12-23 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
BE1028339B1 (fr) * 2020-11-09 2021-12-21 A Mansia Biotech Composition comprenant de l'Akkermansia muciniphila et de l'extrait de thé vert
KR20230118570A (ko) 2020-11-11 2023-08-11 노틸러스 서브시디어리, 인크. 강화된 결합 및 검출 특성을 갖는 친화성 시약
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555B1 (en) 2020-12-21 2026-02-25 10X Genomics, Inc. Methods for capturing probes and/or barcodes
KR20230142832A (ko) 2021-01-08 2023-10-11 셀라노메, 인크. 생물학적 샘플을 분석하기 위한 디바이스 및 방법
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
TW202243733A (zh) * 2021-01-13 2022-11-16 美商加州太平洋生物科學公司 經膠態組裝之表面結構化
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4301499A4 (en) 2021-03-05 2025-01-22 Enumerix, Inc. SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
US20220301657A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
ES3008686T3 (en) 2021-03-18 2025-03-24 10X Genomics Inc Multiplex capture of gene and protein expression from a biological sample
AU2022245985A1 (en) 2021-03-22 2023-09-21 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US20220336057A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Efficient voxelization for deep learning
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12176070B2 (en) * 2021-04-19 2024-12-24 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4347879B1 (en) 2021-06-03 2025-02-19 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
JP2024520804A (ja) * 2021-06-11 2024-05-24 セラノーム， インコーポレイテッド 生体試料を分析するためのシステムおよび方法
WO2022265994A1 (en) 2021-06-15 2022-12-22 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11535892B1 (en) 2021-06-17 2022-12-27 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
EP4374343B1 (en) 2021-07-19 2025-12-03 Illumina, Inc. Intensity extraction with interpolation and adaptation for base calling
CN117813391A (zh) 2021-07-23 2024-04-02 因美纳有限公司 制备用于dna测序的基底表面的方法
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
CN118043476A (zh) * 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
AU2022341171B2 (en) 2021-09-09 2026-02-12 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
CA3234961A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023091592A1 (en) * 2021-11-19 2023-05-25 Dovetail Genomics, Llc Dendrimers for genomic analysis methods and compositions
WO2023102336A1 (en) 2021-11-30 2023-06-08 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023107719A2 (en) * 2021-12-10 2023-06-15 Element Biosciences, Inc. Primary analysis in next generation sequencing
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
EP4565278A1 (en) 2022-08-03 2025-06-11 Nautilus Subsidiary, Inc. Chemical modification of antibodies and functional fragments thereof
CA3264906A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. METHODS FOR PREPARING NUCLEIC ACID NANOSTRUCTURES USING COMPACTING OLIGONUCLEOTIDES
WO2024040068A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
US12365892B2 (en) 2022-09-12 2025-07-22 Element Biosciences, Inc. Double-stranded splint adaptors with universal long splint strands and methods of use
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
CN115409174B (zh) * 2022-11-01 2023-03-31 之江实验室 一种基于dram存内计算的碱基序列过滤方法与装置
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
WO2024151373A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
US20240417720A1 (en) 2023-06-14 2024-12-19 Nautilus Subsidiary, Inc. Making and using structured nucleic acid particles
WO2024263696A1 (en) 2023-06-21 2024-12-26 Nautilus Subsidiary, Inc. Compositions and methods for improving affinity reagent avidity
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025072800A2 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
US20250306015A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Single molecule-resolved characterization of affinity reagent kinetics and thermodynamics
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
US12467087B1 (en) 2024-06-25 2025-11-11 Guardant Health, Inc. Sequencing methods with partitioning
US20260049981A1 (en) 2024-06-27 2026-02-19 Nautilus Subsidiary, Inc. Enrichment of particle reagents for array-based assays
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing

Family Cites Families (440)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3356882A (en) 1965-10-21 1967-12-05 Ford Motor Co Spark plug having the center electrode sheath with a nickel alloy
US3958144A (en) 1973-10-01 1976-05-18 Franks Harry E Spark plug
JPS5180845A (en) 1975-01-14 1976-07-15 Teijin Ltd 2*22 bisu * 3*55 jiburomu 44 * 22 hidorokishetokishi * fueniru * puropannoyojuhojiho
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
JPS5965108A (ja) 1982-10-06 1984-04-13 Sumikin Kozai Kogyo Kk 鋼製合掌堰堤とその構築方法
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4605735A (en) 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US4719179A (en) 1984-11-30 1988-01-12 Pharmacia P-L Biochemicals, Inc. Six base oligonucleotide linkers and methods for their use
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4925785A (en) 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
US5091519A (en) 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4725254A (en) 1986-11-24 1988-02-16 Allied Corporation Method for manufacturing a center electrode for a spark plug
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5354657A (en) 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
DE3813278A1 (de) 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum nachweis von nukleinsaeuren
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5066580A (en) 1988-08-31 1991-11-19 Becton Dickinson And Company Xanthene dyes that emit to the red of fluorescein
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5194599A (en) 1988-09-23 1993-03-16 Gilead Sciences, Inc. Hydrogen phosphonodithioate compositions
DE3836656A1 (de) 1988-10-27 1990-05-03 Boehringer Mannheim Gmbh Neue digoxigenin-derivate und ihre verwendung
US5091302A (en) * 1989-04-27 1992-02-25 The Blood Center Of Southeastern Wisconsin, Inc. Polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic applications thereof
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6379895B1 (en) * 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
DE3924454A1 (de) 1989-07-24 1991-02-07 Cornelis P Prof Dr Hollenberg Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips)
JPH03101086A (ja) 1989-09-14 1991-04-25 Ngk Spark Plug Co Ltd 内燃機関用スパークプラグ
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5003097A (en) 1989-10-02 1991-03-26 The United States Of America As Represented By The Department Of Health And Human Services Method for the sulfurization of phosphorous groups in compounds
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5166387A (en) 1990-01-12 1992-11-24 Applied Biosystems, Inc. Method of synthesizing sulfurized oligonucleotide analogs with thiuram disulfides
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5151510A (en) 1990-04-20 1992-09-29 Applied Biosystems, Inc. Method of synethesizing sulfurized oligonucleotide analogs
GB9009980D0 (en) 1990-05-03 1990-06-27 Amersham Int Plc Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
US5073562A (en) 1990-05-10 1991-12-17 G. D. Searle & Co. Alkoxy-substituted dihydrobenzopyran-2-carboxylic acids and derivatives thereof
US5091320A (en) * 1990-06-15 1992-02-25 Bell Communications Research, Inc. Ellipsometric control of material growth
JPH06500014A (ja) 1990-07-25 1994-01-06 シンジーン，インコーポレイテッド 多数の核酸相補体を生成させる環状伸長法
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3080178B2 (ja) 1991-02-18 2000-08-21 東洋紡績株式会社 核酸配列の増幅方法およびそのための試薬キット
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
JP3085409B2 (ja) 1991-03-29 2000-09-11 東洋紡績株式会社 標的核酸配列の検出方法およびそのための試薬キット
US6589726B1 (en) * 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
ATE148889T1 (de) 1991-09-18 1997-02-15 Affymax Tech Nv Verfahren zur synthese der verschiedenen sammlungen von oligomeren
ZA927323B (en) 1991-09-24 1993-08-30 Keygene Nv Selective restriction fragment amplification: A general method for DNA fingerprinting
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
EP1588761A3 (en) 1991-11-22 2005-11-23 Affymetrix, Inc. Method of forming arrays of polymers
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
AU3728093A (en) 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
GB9214873D0 (en) 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5834202A (en) * 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5583211A (en) 1992-10-29 1996-12-10 Beckman Instruments, Inc. Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
DE69433010T2 (de) 1993-04-12 2004-06-09 Northwestern University, Evanston Verfahren zur darstellung von oligonukleotiden
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5837832A (en) * 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
JPH09500378A (ja) 1993-07-02 1997-01-14 リンクス セラピューティクス，インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成
US5473060A (en) 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
ATE257861T1 (de) 1993-09-27 2004-01-15 Arch Dev Corp Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
US5472672A (en) 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
AU8126694A (en) 1993-10-26 1995-05-22 Affymax Technologies N.V. Arrays of nucleic acid probes on biological chips
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
GB2285942A (en) 1994-01-25 1995-08-02 Ford Motor Co Forming an erosion resistant coating on an electrode
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
WO1995030774A1 (en) 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US7625697B2 (en) 1994-06-17 2009-12-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for constructing subarrays and subarrays made thereby
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6654505B2 (en) * 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
FR2726286B1 (fr) * 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5744367A (en) 1994-11-10 1998-04-28 Igen International, Inc. Magnetic particle based electrochemiluminescent detection apparatus and method
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5866337A (en) * 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
EP0824684B1 (en) 1995-05-12 2007-11-07 Novartis AG Method for the parallel detection of a plurality of analytes using evanescently excited luminescence
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
US5774305A (en) 1995-06-07 1998-06-30 Seagate Technology, Inc. Head gimbal assembly to reduce slider distortion due to thermal stress
US5675209A (en) 1995-06-19 1997-10-07 Hoskins Manufacturing Company Electrode material for a spark plug
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US6132580A (en) 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5658734A (en) 1995-10-17 1997-08-19 International Business Machines Corporation Process for synthesizing chemical compounds
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
ES2262902T3 (es) * 1995-12-05 2006-12-01 Jorn Erland Koch Una reaccion en cascada de amplificacion de acido nucleico.
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6660233B1 (en) 1996-01-16 2003-12-09 Beckman Coulter, Inc. Analytical biochemistry system with robotically carried bioarray
EP0894145B1 (en) * 1996-03-01 2005-09-21 The University Court Of The University Of Dundee Drug trial assay system
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
JP2000512744A (ja) 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド 標識材料を検出するシステムおよび方法
EP0912766B2 (en) 1996-06-04 2011-12-14 University of Utah Research Foundation Monitoring hybridization during pcr
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
ATE211258T1 (de) 1996-09-04 2002-01-15 Scandinavian Micro Biodevices Mikrofliesssystem für die teilchenanalyse und trennung
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
AU761161B2 (en) 1996-11-06 2003-05-29 Sequenom, Inc. High density immobilization of nucleic acids
DE19782096T1 (de) 1996-11-06 2000-03-23 Sequenom Inc Immobiliserung von Nucleinsäuren in hoher Dichte
DE69720041T2 (de) 1996-11-14 2004-01-08 Affymetrix, Inc., Santa Clara Chemische amplifizierung zur synthese von musterordnungen
US6054100A (en) * 1996-11-18 2000-04-25 Robbins Scientific Corporation Apparatus for multi-well microscale synthesis
US5916750A (en) * 1997-01-08 1999-06-29 Biogenex Laboratories Multifunctional linking reagents for synthesis of branched oligomers
US6297006B1 (en) * 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US20020042048A1 (en) 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US5994068A (en) 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
EP0972081B1 (en) 1997-04-01 2007-06-13 Solexa Ltd. Method of nucleic acid amplification
EP0975802B1 (en) 1997-04-01 2004-06-23 Manteia S.A. Method of nucleic acid sequencing
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US20040229221A1 (en) 1997-05-08 2004-11-18 Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
US5919626A (en) † 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
GB2342094B (en) 1997-07-07 2002-10-23 Medical Res Council In vitro sorting method
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
AU737174B2 (en) 1997-10-10 2001-08-09 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
WO1999023254A1 (en) 1997-10-31 1999-05-14 Affymetrix, Inc. Expression profiles in adult and fetal organs
US5866473A (en) * 1997-10-31 1999-02-02 Advanced Micro Devices, Inc. Method of manufacturing a polysilicon gate having a dimension below the photolithography limitation
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US20030039967A1 (en) 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
US6087102A (en) 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
JP2002502588A (ja) 1998-02-06 2002-01-29 アフィメトリックス インコーポレイテッド 製造プロセスにおける品質管理の方法
US6136537A (en) 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US6174687B1 (en) 1999-02-26 2001-01-16 The Burnham Institute Methods of identifying lung homing molecules using membrane dipeptidase
DE19812729A1 (de) 1998-03-24 1999-09-30 Bosch Gmbh Robert Elektromotor, insbesondere mit einem Lüfterrad zur Bildung eines Axial- oder Radiallüfters
DE69940970D1 (de) * 1998-03-25 2009-07-23 Olink Ab Rolling circle replikation von padlock-sonden
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6004755A (en) * 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
US6284497B1 (en) * 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
JP4262799B2 (ja) 1998-04-16 2009-05-13 平田機工株式会社 生タイヤ供給方法
US6355419B1 (en) * 1998-04-27 2002-03-12 Hyseq, Inc. Preparation of pools of nucleic acids based on representation in a sample
US6270831B2 (en) 1998-04-30 2001-08-07 Medquest Products, Inc. Method and apparatus for providing a conductive, amorphous non-stick coating
US6255469B1 (en) 1998-05-06 2001-07-03 New York University Periodic two and three dimensional nucleic acid structures
US6031078A (en) 1998-06-16 2000-02-29 Millennium Pharmaceuticals, Inc. MTbx protein and nucleic acid molecules and uses therefor
US5980345A (en) 1998-07-13 1999-11-09 Alliedsignal Inc. Spark plug electrode having iridium based sphere and method for manufacturing same
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
US6265021B1 (en) 1998-07-31 2001-07-24 International Business Machines Corporation Nanoparticle structures utilizing synthetic DNA lattices
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
US6046039A (en) 1998-08-19 2000-04-04 Battelle Memorial Institute Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product
US6653077B1 (en) * 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6287824B1 (en) * 1998-09-15 2001-09-11 Yale University Molecular cloning using rolling circle amplification
US20020019007A1 (en) * 1998-09-18 2002-02-14 Jensen Wayne A. PCR methods and materials
US6326144B1 (en) 1998-09-18 2001-12-04 Massachusetts Institute Of Technology Biological applications of quantum dots
US6426513B1 (en) 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US7272507B2 (en) * 1999-10-26 2007-09-18 Michael Paul Strathmann Applications of parallel genomic analysis
DE60042775D1 (de) 1999-01-06 2009-10-01 Callida Genomics Inc Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6514768B1 (en) 1999-01-29 2003-02-04 Surmodics, Inc. Replicable probe array
EP1147229A2 (en) * 1999-02-02 2001-10-24 Bernhard O. Palsson Methods for identifying drug targets based on genomic sequence data
JP2004512807A (ja) 1999-03-18 2004-04-30 コンプリート　ゲノミックス　エイエス 可読情報を有するフラグメント鎖のクローニングおよび生成方法
JP2003527075A (ja) 1999-03-25 2003-09-16 ハイセック，インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
JP2002539849A (ja) 1999-03-26 2002-11-26 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ ユニバーサルアレイ
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
EP1196630B2 (en) 1999-04-20 2018-10-17 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
EP1179185B1 (en) 1999-05-07 2009-08-12 Life Technologies Corporation A method of detecting an analyte using semiconductor nanocrystals
CA2374390A1 (en) * 1999-05-20 2000-12-14 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
CA2374596A1 (en) 1999-05-20 2000-11-30 Illumina, Inc. Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US6326719B1 (en) 1999-06-16 2001-12-04 Alliedsignal Inc. Spark plug shell having a bimetallic ground electrode spark plug incorporating the shell, and method of making same
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
AU6387000A (en) 1999-07-29 2001-02-19 Genzyme Corporation Serial analysis of genetic alterations
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
CA2386791A1 (en) * 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6287778B1 (en) 1999-10-19 2001-09-11 Affymetrix, Inc. Allele detection using primer extension with sequence-coded identity tags
CN1402796A (zh) 1999-11-02 2003-03-12 胡元徽 分子微阵列及其制备方法和用途
JP2003515149A (ja) * 1999-11-26 2003-04-22 キュラジェン コーポレイション 核酸プローブアレイ
WO2001040516A2 (en) * 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US20030186256A1 (en) 1999-12-23 2003-10-02 Achim Fischer Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
AU2282801A (en) 1999-12-29 2001-07-09 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US6221603B1 (en) * 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
AU2001238067B2 (en) * 2000-02-07 2007-01-25 Illumina, Inc. Nucleic acid detection methods using universal priming
JP2003521252A (ja) * 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2001062982A2 (en) 2000-02-25 2001-08-30 Mosaic Technologies, Inc. Methods for multi-stage solid phase amplification of nucleic acids
US20020004204A1 (en) 2000-02-29 2002-01-10 O'keefe Matthew T. Microarray substrate with integrated photodetector and methods of use thereof
US6413722B1 (en) * 2000-03-22 2002-07-02 Incyte Genomics, Inc. Polymer coated surfaces for microarray applications
CA2405629C (en) * 2000-04-10 2016-11-22 Matthew Ashby Methods for the survey and genetic analysis of populations
WO2001083826A2 (en) 2000-05-03 2001-11-08 Massachusetts Institute Of Technology Methods and reagents for assembling molecules on solid supports
EP1290225A4 (en) 2000-05-20 2004-09-15 Univ Michigan METHOD FOR PRODUCING A DNA BANK BY POSITIONAL REPRODUCTION
EP1286226B1 (en) 2000-05-25 2008-02-27 Fuji Xerox Co., Ltd. Toner and image forming method
DE10027651C2 (de) 2000-06-03 2002-11-28 Bosch Gmbh Robert Elektrode, Verfahren zu deren Herstellung und Zündkerze mit einer derartigen Elektrode
US6660229B2 (en) 2000-06-13 2003-12-09 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
JP3930227B2 (ja) * 2000-06-14 2007-06-13 ペンタックス株式会社 磁気式エンコーダおよび磁気式エンコーダを搭載した測量機
JP2002085097A (ja) 2000-09-12 2002-03-26 Hitachi Ltd Ｄｎａ塩基配列決定法
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
DE60131903T2 (de) 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
US20020055102A1 (en) * 2000-10-24 2002-05-09 David Stern Apparatus and method for scanning multiple arrays of biological probes
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
US20020074920A1 (en) 2000-12-15 2002-06-20 Chiu Randolph Kwok-Kin High efficiency, extended life spark plug having improved firing tips
WO2002050310A2 (en) * 2000-12-20 2002-06-27 The Regents Of The University Of California Rolling circle amplification detection of rna and dna
WO2002053732A2 (en) * 2000-12-28 2002-07-11 Pangenex Methods for making polynucleotide libraries, polynucleotide arrays, and cell librraries for high-throughput genomics analysis
EP1808496B2 (en) 2001-01-30 2021-12-22 Illumina Cambridge Limited Methods of sequencing polynucleotide arrays, and preparation methods therefor
WO2002061143A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Comparative analysis of nucleic acids using population tagging
JP4171206B2 (ja) 2001-03-16 2008-10-22 株式会社デンソー スパークプラグおよびその製造方法
EP2465943A3 (en) * 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
GB0114853D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happier Mapping
CA2453450A1 (en) 2001-07-20 2003-11-06 Life Technologies Corporation Luminescent nanoparticles and methods for their preparation
AU2002337653A1 (en) 2001-07-25 2003-02-17 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
CA2453840A1 (en) 2001-07-27 2003-02-13 Mcgill University Isp-1 and ctb-1 genes and uses thereof
US6881579B2 (en) 2001-07-30 2005-04-19 Agilent Technologies, Inc. Sample processing apparatus and methods
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
WO2005016869A1 (en) * 2003-08-19 2005-02-24 Postech Foundation Novel dendrimer compound, a biochip using the same and a fabricating method thereof
WO2005040094A1 (en) * 2003-10-24 2005-05-06 Postech Foundation Novel dendrimer compound and a biochip using the same
US9201067B2 (en) * 2003-03-05 2015-12-01 Posco Size-controlled macromolecule
US6975943B2 (en) * 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
GB0126887D0 (en) 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
US7208295B2 (en) 2001-11-19 2007-04-24 Affymetrix, Inc. Multiplex oligonucleotide addition and target amplification
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
US7361310B1 (en) * 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
US7011945B2 (en) * 2001-12-21 2006-03-14 Eastman Kodak Company Random array of micro-spheres for the analysis of nucleic acids
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
AU2003279697A1 (en) 2002-05-17 2004-02-16 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilizaton of nucleic acids
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
AUPS298102A0 (en) 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
US20050019776A1 (en) * 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
US7046376B2 (en) 2002-07-05 2006-05-16 Therma-Wave, Inc. Overlay targets with isolated, critical-dimension features and apparatus to measure overlay
US6747331B2 (en) * 2002-07-17 2004-06-08 International Business Machines Corporation Method and packaging structure for optimizing warpage of flip chip organic packages
TW200403052A (en) 2002-07-17 2004-03-01 Novartis Ag Use of organic compounds
AU2003268110A1 (en) * 2002-08-16 2004-03-03 Miragene, Inc. Integrated system for printing, processing, and imaging protein microarrays
ES2407681T3 (es) 2002-08-23 2013-06-13 Illumina Cambridge Limited Nucleótidos modificados para la secuenciación de polinucleótidos.
WO2004025751A2 (en) 2002-09-11 2004-03-25 Moltech Invent S.A. Non-carbon anodes for aluminium electrowinning and other oxidation resistant components with iron oxide-containing coatings
EP1556506A1 (en) 2002-09-19 2005-07-27 The Chancellor, Masters And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US20040110213A1 (en) 2002-09-30 2004-06-10 Eugeni Namsaraev Polynucleotide synthesis and labeling by kinetic sampling ligation
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US20040120861A1 (en) * 2002-10-11 2004-06-24 Affymetrix, Inc. System and method for high-throughput processing of biological probe arrays
ATE483822T1 (de) 2002-10-11 2010-10-15 Univ Erasmus Primer für nukleinsäureamplifikation in pcr- basierten klonalitätsstudien
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
JP4395133B2 (ja) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
WO2004076683A2 (en) 2003-02-26 2004-09-10 Callida Genomics, Inc. Random array dna analysis by hybridization
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
DE10300599A1 (de) 2003-01-10 2004-07-22 Jörg Dr. Sommer Mehrrumpfiges Schiff mit beweglichen Schwimmkörpern als Wellenkraftwerk
CN101128601B (zh) 2003-01-29 2011-06-08 454生命科学公司 核酸扩增和测序的方法
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP2006517798A (ja) 2003-02-12 2006-08-03 イェニソン スベンスカ アクティエボラーグ 核酸配列用の方法及び手段
DK2374900T3 (da) 2003-03-07 2016-10-17 Rubicon Genomics Inc Polynukleotider til amplifikation og analyse af samlet genom- og samlet transkriptions-biblioteker, genereret ved en DNA-polymeriseringsproces
FR2852605B1 (fr) 2003-03-18 2012-11-30 Commissariat Energie Atomique Procede de preparation de fragments d'adn et ses applications
JP2004286579A (ja) 2003-03-20 2004-10-14 Kanagawa Acad Of Sci & Technol Ｄｎａ分析用アレイおよびそれを用いたｄｎａ分析システムおよび分析方法
WO2004087916A1 (ja) 2003-03-28 2004-10-14 Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities ｃＤＮＡ合成方法
WO2004092331A2 (en) * 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
KR100523767B1 (ko) 2003-06-12 2005-10-26 한국과학기술원 유기 초분자의 자기조립과 자외선 에칭을 이용한나노패턴의 형성방법
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
US7416911B2 (en) 2003-06-24 2008-08-26 California Institute Of Technology Electrochemical method for attaching molecular and biomolecular structures to semiconductor microstructures and nanostructures
JP4773955B2 (ja) 2003-06-30 2011-09-14 ロウステック プロプライエタリー リミテッド 空間選択的堆積によるミクロスケール及びナノスケールの加工及び製造
AU2003903295A0 (en) 2003-06-30 2003-07-10 Raustech Pty Ltd Substrate for combinatorial chemistry
WO2005048283A2 (en) 2003-07-18 2005-05-26 Northwestern University Surface and site-specific polymerization by direct-write lithography
GB0320337D0 (en) 2003-08-29 2003-10-01 Syrris Ltd A microfluidic system
AU2004286201B2 (en) 2003-09-10 2010-09-09 Altheadx, Inc. Expression profiling using microarrays
US7781378B2 (en) 2003-09-12 2010-08-24 Cornell Research Foundation, Inc. Protective coating for array material deposition
AT501110A1 (de) * 2003-09-16 2006-06-15 Upper Austrian Res Gmbh Arrays zur bindung von molekülen
US8222005B2 (en) * 2003-09-17 2012-07-17 Agency For Science, Technology And Research Method for gene identification signature (GIS) analysis
WO2005029040A2 (en) 2003-09-18 2005-03-31 Parallele Biosciences, Inc. System and methods for enhancing signal-to-noise ratios of microarray-based measurements
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US20050221341A1 (en) 2003-10-22 2005-10-06 Shimkets Richard A Sequence-based karyotyping
TWM245024U (en) * 2003-10-30 2004-10-01 Tranmax Machinery Co Ltd Airtight structure for air passage of pneumatic tool
US7148361B2 (en) 2003-10-31 2006-12-12 North Carolina State University Synthesis of phosphono-substituted porphyrin compounds for attachment to metal oxide surfaces
JP3796607B2 (ja) 2003-10-31 2006-07-12 株式会社リコー 定着装置
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7854108B2 (en) 2003-12-12 2010-12-21 Vision Robotics Corporation Agricultural robot system and method
US7972994B2 (en) * 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
US20050136414A1 (en) 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
AU2004311882A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
EP3673986A1 (en) * 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP1735458B1 (en) 2004-01-28 2013-07-24 454 Life Sciences Corporation Nucleic acid amplification with continuous flow emulsion
GB0402895D0 (en) 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
CN1950519A (zh) 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US20050214840A1 (en) 2004-03-23 2005-09-29 Xiangning Chen Restriction enzyme mediated method of multiplex genotyping
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US7470517B2 (en) 2004-04-09 2008-12-30 Trustees Of Boston University Method for de novo detection of sequences in nucleic acids: target sequencing by fragmentation
WO2005112565A2 (en) 2004-04-28 2005-12-01 University Of Houston Preparation of nanometric arrays of biomolecules on oligo- or poly(ethylene glycol) films on silicon surfaces
US20050260609A1 (en) 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
EP1766090B1 (en) * 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US7565346B2 (en) 2004-05-31 2009-07-21 International Business Machines Corporation System and method for sequence-based subspace pattern clustering
US20060024711A1 (en) * 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
EP1647600A3 (en) 2004-09-17 2006-06-28 Affymetrix, Inc. (A US Entity) Methods for identifying biological samples by addition of nucleic acid bar-code tags
US20080050659A1 (en) 2004-09-30 2008-02-28 Japan Science And Technology Agency Method of Patterning Self-Organizing Material, Patterned Substrate of Self-Organizing Material and Method of Producing the Same, and Photomask Using Patterned Substrate of Self-Organizing Material
GB0422551D0 (en) 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
US20060110764A1 (en) 2004-10-25 2006-05-25 Tom Tang Large-scale parallelized DNA sequencing
CA2588122A1 (en) 2004-11-16 2006-05-26 Helicos Biosciences Corporation Tirf single molecule analysis and method of sequencing nucleic acids
EP1812036A2 (en) 2004-11-17 2007-08-01 The University Of Chicago Histone deacetylase inhibitors and methods of use
WO2006061600A1 (en) 2004-12-07 2006-06-15 University Of Southampton Particles for use as a solid support and process for their preparation
EP1879906A4 (en) 2005-01-05 2009-07-22 Advanced Genetic Analysis Corp REVERSIBLE NUCLEOTIDE TERMINATORS AND USES THEREOF
US20080003571A1 (en) 2005-02-01 2008-01-03 Mckernan Kevin Reagents, methods, and libraries for bead-based sequencing
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8569042B2 (en) 2005-02-23 2013-10-29 Alcatel Lucent DNA structures on ferroelectrics and semiconductors
US20060223122A1 (en) 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
US8110196B2 (en) 2005-04-29 2012-02-07 Polytopas LLC Methods and compositions for polytopic vaccination
US20060263789A1 (en) * 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
DK1907571T3 (da) 2005-06-15 2017-08-21 Complete Genomics Inc Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter
CA2616433A1 (en) 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US20090291419A1 (en) * 2005-08-01 2009-11-26 Kazuaki Uekawa System of sound representaion and pronunciation techniques for english and other european languages
JP2009503555A (ja) 2005-08-04 2009-01-29 ヘリコス バイオサイエンシーズ コーポレイション マルチチャネルフローセル
DK1915446T3 (da) 2005-08-11 2017-09-11 Synthetic Genomics Inc In vitro rekombination fremgangsmåde
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
DK1929039T4 (en) 2005-09-29 2014-02-17 Keygene Nv High throughput-screening af mutageniserede populationer
AU2013202990B2 (en) 2005-10-07 2015-08-20 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
CA2624896C (en) 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
AU2012216376B2 (en) 2005-10-07 2015-08-13 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US9012193B2 (en) 2005-10-10 2015-04-21 The Regents Of The University Of California Micro-bubble plate for patterning biological and non-biological materials
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
EP1987162A4 (en) * 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007091077A1 (en) 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
US8460878B2 (en) * 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
EP1994180A4 (en) 2006-02-24 2009-11-25 Callida Genomics Inc GENOME SEQUENCING ON DNA ARRAYS WITH HIGH THROUGHPUT
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
JP2009529876A (ja) * 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段
DE102006015433A1 (de) 2006-03-31 2007-10-04 Reuver, Hermannus S.F. Verfahren zur Aufbereitung von Rotschlamm in Böden sowie Anlage zur Durchführung des Verfahrens
US7359610B2 (en) 2006-04-03 2008-04-15 Adc Telecommunications, Inc. Cable manager including nestable radius limiter
US7569979B2 (en) 2006-04-07 2009-08-04 Federal-Mogul World Wide, Inc. Spark plug having spark portion provided with a base material and a protective material
WO2007121489A2 (en) 2006-04-19 2007-10-25 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8725425B2 (en) 2007-01-26 2014-05-13 Illumina, Inc. Image data efficient genetic sequencing method and system
US8673563B2 (en) 2007-06-08 2014-03-18 Bio-Dixam, Llc Amplification method of methylated or unmethylated nucleic acid
WO2009037266A2 (en) 2007-09-17 2009-03-26 Universite Louis Pasteur Method for detecting or quantifying a truncating mutation
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7901890B2 (en) * 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
JP2011509095A (ja) 2008-01-09 2011-03-24 ライフ テクノロジーズ コーポレーション 核酸配列決定のための対をなすタグのライブラリーを製造する方法
US20090298131A1 (en) 2008-02-19 2009-12-03 Intelligent Biosystems, Inc. Non-Emulsion Methods And Masked Biomolecules
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US9056519B2 (en) 2008-07-18 2015-06-16 Ideapaint, Inc. Ambient cure solvent-based coatings for writable-erasable surfaces
US8407554B2 (en) 2009-02-03 2013-03-26 Complete Genomics, Inc. Method and apparatus for quantification of DNA sequencing quality and construction of a characterizable model system using Reed-Solomon codes
US9524369B2 (en) * 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
EP2529026B1 (en) 2010-01-25 2013-11-13 Rd Biosciences Inc. Self-folding amplification of target nucleic acid
EP2531608B1 (en) 2010-02-05 2020-08-19 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
ES2623859T3 (es) 2010-03-04 2017-07-12 Miacom Diagnostics Gmbh FISH múltiple mejorada
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
US8902293B2 (en) 2011-01-17 2014-12-02 Panasonic Corporation Imaging device
CN107368705B (zh) * 2011-04-14 2021-07-13 完整基因有限公司 分析生物体的基因组dna的方法和计算机系统
WO2012162161A1 (en) 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
EP2771103B1 (en) 2011-10-28 2017-08-16 Illumina, Inc. Microarray fabrication system and method
US9006630B2 (en) 2012-01-13 2015-04-14 Altasens, Inc. Quality of optically black reference pixels in CMOS iSoCs
ES2928691T3 (es) 2012-05-21 2022-11-22 Scripps Research Inst Métodos de preparación de muestras
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11414702B2 (en) 2005-06-15 2022-08-16 Complete Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US12421544B2 (en) 2005-06-15 2025-09-23 Complete Genomics, Inc. Labeling strategy for use in DNA sequencing to facilitate assembly of sequence reads into longer fragments of a genome

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