DK1907571T3 - Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter - Google Patents

Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter Download PDF

Info

Publication number
DK1907571T3
DK1907571T3 DK06760745.7T DK06760745T DK1907571T3 DK 1907571 T3 DK1907571 T3 DK 1907571T3 DK 06760745 T DK06760745 T DK 06760745T DK 1907571 T3 DK1907571 T3 DK 1907571T3
Authority
DK
Denmark
Prior art keywords
fragments
size
sequence
target
probes
Prior art date
Application number
DK06760745.7T
Other languages
English (en)
Inventor
Radoje Drmanac
Original Assignee
Complete Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37571035&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1907571(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Complete Genomics Inc filed Critical Complete Genomics Inc
Application granted granted Critical
Publication of DK1907571T3 publication Critical patent/DK1907571T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/313—Branched oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/778—Nanostructure within specified host or matrix material, e.g. nanocomposite films
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/788—Of specified organic or carbon-based composition
    • Y10S977/789—Of specified organic or carbon-based composition in array format
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/788—Of specified organic or carbon-based composition
    • Y10S977/789—Of specified organic or carbon-based composition in array format
    • Y10S977/79—Of specified organic or carbon-based composition in array format with heterogeneous nanostructures
    • Y10S977/791—Molecular array
    • Y10S977/792—Nucleic acid array, e.g. human genome array
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/88—Manufacture, treatment, or detection of nanostructure with arrangement, process, or apparatus for testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/84—Manufacture, treatment, or detection of nanostructure
    • Y10S977/882—Assembling of separate components, e.g. by attaching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (33)

1. Fremgangsmåde til sekventering af et eller flere målpolynukleotider, hvilken fremgangsmåde omfatter trinene: tilfældig fragmentering af den ene eller de flere målpolynukleotider, der er til stede, i en forudbestemt dækningsmængde til frembringelse af en population, der indeholder overlappende fragmenter med en første størrelse, der hver har en gennemsnitslængde, der er mindre end gennemsnitslængderne af målpolynukleotiderne; frembringelse af et antal separate blandinger fra populationen af fragmenter med en første størrelse, idet et sådant antal vælges, således at mindst 60 % af sådanne separate blandinger kun indeholder ikke-overlappende fragmenter, og således at udgangsblandingen for hvert sådant fragment med en første størrelse er bestemmelig; sekventering af mindst en del af et eller flere fragmenter med en første størrelse i hver separate blanding til opnåelse af sekvensresultater; og samling af sekvensresultaterne fra de separate blanding til komplette eller partielle nukleotidsekvenser for det ene eller de flere målpolynukleotider til frembringelse af contigs, hvor en sådan samling afhænger af udgangsblandingen for mindst en del af sekvensinformationen.
2. Fremgangsmåde ifølge krav 1, hvor trinet frembringelse indbefatter replikering af hvert af fragmenterne med den første størrelse i de separate blandinger.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor hvert af sekvensresultaterne fra den ene eller de flere fragmenter med den første størrelse i hver separate blanding har en længde, der er mindre end længderne af fragmenterne med den første størrelse, og antallet af sekvensresultater vælges, således at mindst 30 % af fragmenterne med den første størrelse sekventeres.
4. Fremgangsmåde ifølge kravene 1 til 3, der yderligere indbefatter et trin med tagging af hvert af fragmenterne med den første størrelse med et oligonukleotidtag, således at oligonukleotidtagget identificerer udgangsblandingen for fragmentet med den første størrelse.
5. Fremgangsmåde ifølge krav 4, hvor trinet opnåelse af sekvensresultater fra et eller flere fragmenter med den første størrelse i hver separate blanding indbefatter bestemmelse af nukleotidsekvensen for oligonukleotidtagget, og hvor eventuelt opnåelsen af sekvensresultater omfatter sekventering med højt gennemløb.
6. Fremgangsmåde ifølge krav 3, hvor trinet opnåelse af sekvensresultater indbefatter: generering for hver af de separate blandinger af en flerhed af målconcatemerer fra fragmenterne med den første størrelse, idet hver målconcatemer omfatter flere kopier af en del af et fragment med en første størrelse, og hver flerhed af målconcatemerer indbefatter et antal af sådanne dele, der dækker fragmentet med den første størrelse; frembringelse for hver af de separate blandinger af et tilfældigt array af målconcatemerer, der er fikseret til en overflade ved en densitet, således at mindst størstedelen af målconcatemererne kan opløses optisk; og opnåelse af sekvensresultater fra målconcatemerer for hver af de separate blandinger, hvor sådanne sekvensresultater hver har en længde, der er mindre end længderne af fragmenterne med den første størrelse, og hver af sådanne antal af sekvensresultater er valgt, således at sekvensresultaterne for hver separate blanding dækker fragmenterne med den første størrelse deri.
7. Fremgangsmåde ifølge krav 6, hvor trinet opnåelse af sekvensresultater omfatter: (a) hybridisering af en eller flere prober fra et første sæt af prober til hvert tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (b) hybridisering af en eller flere prober fra et andet sæt af prober til det tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (c) ligering af prober fra det første og andet sæt, der er hybridiseret til en målconcatemer på fortløbende steder; (d) identificering af sekvenserne af det ligerede første og andet sæt af prober; og (e) gentagelse af trinene (a) til (d) for hvert tilfældige array, indtil sekvenserne af fragmenterne med den første størrelse i hver af de separate blandinger er bestemt ud fra identiteterne af sekvenserne af de ligerede prober.
8. Fremgangsmåde ifølge kravene 1 til 7, hvor det ene eller de flere målpolynukleotider indeholder repetitive sekvensregioner, der hver har en længde, der er større end længderne af sekvensresultaterne.
9. Fremgangsmåde ifølge krav 2, hvor gennemsnitslængden af fragmenterne med den første størrelse ligger i intervallet fra 50 til 2000 baser.
10. Fremgangsmåde ifølge kravene 1 til 9, hvor gennemsnitslængden af fragmenterne med den første størrelse ligger i intervallet fra en tredjedel til en tusindedel af længden af det ene eller de flere målpolynukleotider.
11. Fremgangsmåde ifølge krav 1, der yderligere omfatter: tilfældig fragmentering af hver af fragmenterne med den første størrelse i hver af blandingerne til frembringelse af en population af fragmenter med en anden størrelse for hver blanding, således at fragmenterne med en anden størrelse har en gennemsnitslængde, der er mindre end længderne af fragmenterne med den første størrelse, og således at udgangsblandingen for hver af fragmenterne med en anden størrelse er bestemmelig.
12. Fremgangsmåde ifølge krav 1, hvor samlingen omfatter: samling af sekvensresultater til rekonstruktion af sekvensen af fragmenter med hver separate blanding; og samling af overlappende fragmenter fra uafhængige separate blandinger til frembringelse af de komplette eller partielle nukleotidsekvenser for målpolynukleotidet eller målpolynukleotiderne
13. Fremgangsmåde ifølge krav 11, hvor fragmenterne med den anden størrelse i de separate blandinger tagges til indikering af udgangsportionen, og samlingen omfatter: gruppering af sekvensresultaterne ved hjælp af tagsekvensen, og samling af sekvens-contigs derfra; samling af overlappende sekvens-contigs fra uafhængige portioner til frembringelse af komplette eller partielle nukleotidsekvenser for målpolynukleotidet eller målpolynukleotiderne.
14. Fremgangsmåde ifølge krav 1, hvor samlingen omfatter: samling af sekvensinformation i hver portion til rekonstruktion af sekvenserne af fragmenterne; samling af sekvenser af fragmenterne til rekonstruktion af sekvensen af målpolynukleotidet i en prøve.
15. Fremgangsmåde ifølge krav 11, hvor gennemsnitslængden af fragmenterne med den første størrelse er mindre end 300 kilobaser, og gennemsnitslængden af fragmenterne med den anden størrelse ligger i intervallet fra 50 til 600 baser.
16. Fremgangsmåde ifølge krav 11, hvor den forudbestemte dækningsmængde ligger i intervallet fra 3 til 30.
17. Fremgangsmåde ifølge kravene 1 til 16, hvor det ene eller de flere målpolynukleotider er et eller flere bakterielle genomer.
18. Fremgangsmåde ifølge kravene 11 til 16, hvor det ene eller de flere målpolynukleotider er haploide strenge af et mammaliagenom.
19. Fremgangsmåde ifølge krav 4, der yderligere omfatter puljning af de taggede fragmenter med den første størrelse i en enkelt blanding før opnåelse af sekvensresultaterne.
20. Fremgangsmåde ifølge krav 19, som er en fremgangsmåde til sekventering med højt gennemløb.
21. Fremgangsmåde ifølge kravene 1 til 20, der yderligere indbefatter separering af fragmenterne med den første størrelse efter størrelse før trinet frembringelse af de separate blandinger.
22. Fremgangsmåde ifølge krav 11, hvor trinet sekventering omfatter trinene: generering for hver af de separate blandinger af en flerhed af målconcatemerer fra fragmenterne med den anden størrelse, idet hver målconcatemer omfatter flere kopier af et fragment med den anden størrelse, og hver flerhed af målconcatemerer indbefatter et antal fragmenter med den anden størrelse, der dækker fragmenterne med den første størrelse i dets respektive separate blanding; frembringelse for hver af de separate blandinger af et tilfældigt array af målconcatemerer, der er fikseret til en overflade ved en densitet, således at mindst størstedelen af målconcatemererne kan opløses optisk; og generering af et antal sekvensresultater fra målconcatemerer i hver af de separate blandinger, idet sådanne sekvensresultater hver har en længde, der er mindre end længderne af fragmenterne med den anden størrelse, og hvert af sådanne antal af sekvensresultater er valgt, således at sekvensresultaterne af hver separate blanding dækker fragmenterne med den anden størrelse deri.
23. Fremgangsmåde ifølge krav 22, hvor trinet opnåelse af sekvensresultaterne omfatter: (a) hybridisering af en eller flere prober fra et første sæt af prober til hvert tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (b) hybridisering af en eller flere prober fra et andet sæt af prober til det tilfældige array under betingelser, der åbner mulighed for frembringelse af perfekt matchede duplexer mellem den ene eller de flere prober og komplementære sekvenser på målconcatemerer; (c) ligering af prober fra det første og andet sæt, der er hybridiseret til en målconcatemer på fortløbende steder; (d) identificering af sekvenserne af det ligerede første og andet sæt af prober; og (e) gentagelse af trinene (a) til (d) for hvert tilfældige array, indtil sekvenserne af fragmenterne med den anden størrelse i hver af de separate blandinger er bestemt ud fra identiteterne af sekvenserne af de ligerede prober.
24. Fremgangsmåde ifølge krav 22 eller 23, hvor hver af fragmenterne med den første størrelse har et oligonukleotidtag bundet til en ende, hvilket oligonukleotidtag identificerer den separate blanding af et sådant fragment med en første størrelse.
25. Fremgangsmåde til sekventering af nukleotidsekvenser af et eller flere målpolynukleotider, hvilken fremgangsmåde omfatter trinene: frembringelse af flere niveauer af blandinger, der omfatter et hierarki af indlejrede fragmenter af det ene eller de flere målpolynukleotider fremstillet ved tilfældig fragmentering, hvor hver blanding af hvert forudgående niveau opdeles i et antal blandinger på et efterfølgende niveau, idet mindst ét niveau har blandinger med ikke-overlappende fragmenter, og det ene eller de flere niveauer har et sidste niveau, hvor blandinger af forudgående niveauer kan identificeres for hvert fragment i hver blanding på det sidste niveau; opnåelse af sekvensresultater fra mindst en del af et eller flere fragmenter i hver blanding på det sidste niveau; og tilvejebringelse af komplette eller partielle nukleotidsekvenser af det ene eller de flere målpolynukleotider ved hjælp af samling af sekvensresultaterne fra det sidste niveau af blandinger til frembringelse af contigs, hvor en sådan samling afhænger af udgangsblandingen på mindst ét af niveauerne.
26. Fremgangsmåde ifølge krav 25, hvor trinet frembringelse indbefatter replikering af fragmenterne i hver af blandingerne på hvert af de efterfølgende niveauer.
27. Fremgangsmåde ifølge krav 25 eller 26, hvor samlingen af sekvensresultaterne afhænger af identiteterne af blandingerne på hvert af det ene eller de flere niveauer.
28. Fremgangsmåde ifølge kravs 25 til 27, der yderligere indbefatter et trin med tagging af hvert af fragmenterne på hvert af niveauerne med et oligonukleotidtag, således at oligonukleotidtagget identificerer udgangsblandingen for hvert af fragmenterne på hvert niveau; og hvor blandingerne forud for trinet opnåelse af sekvensresultater fra det sidste niveau puljes sammen til en enkelt blanding.
29. Fremgangsmåde ifølge krav 28, hvor trinet bestemmelse af nukleotidsekvensen af delen af fragmenterne på den sidste niveau indbefatter sekventering af hvert af oligonukleotidtaggene.
30. Fremgangsmåde ifølge kravene 25 til 29, hvor det ene eller de flere målpolynukleotider indeholder repetitive sekvensregioner, der hver har en længde, der er større end længden af sekvensresultaterne.
31. Fremgangsmåde ifølge kravene 1 til 24, hvor trinet tilfældig fragmentering udføres ved hjælp af en fremgangsmåde udvalgt blandt sonikering, passage gennem kapillarer, dispersion af en DNA-opløsning i fine dråber, behandling med DNase I, behandling med endonuclease og tagget PCR-amplifikation .
32. Fremgangsmåde ifølge kravene 1 til 31, der tilvejebringer haplotype-information for et diploidt genom.
33. Fremgangsmåde ifølge krav 25, hvor der dannes en flerhed af niveauer af blandinger ved hjælp af en proces, der omfatter trinene: (a) tilfældig fragmentering af det ene eller de flere målpolynukleotider, der er til sted, i en forudbestemt dækningsmængde til frembringelse af en population, der indeholder overlappende fragmenter med en første størrelse, som hver har en gennemsnitslængde, der er mindre end længderne af målpolynukleotiderne, idet fragmenterne med den første størrelse er de første fragmenter med forudgående størrelse; (b) generering af et sæt af separate blandinger fra hver population af fragmenter med forudgående størrelse, idet et sådant sæt har et niveau og et antal blandinger deri, hvor hvert sådant antal er valgt, således at mindst 60 % af de separate blandinger kun indeholder ikke-overlappende fragmenter; (c) tilfældig fragmentering af hvert af fragmenterne med den forudgående størrelse i hver af blandingerne til frembringelse af en population af fragmenter med en anden størrelse for hver blanding, således at hvert fragment med den anden størrelse har en gennemsnitslængde, der er mindre end gennemsnitslængderne af fragmenterne med den første størrelse, og således at udgangsblandingerne for hvert fragment med den anden størrelse kan identificeres, idet fragmenterne med den anden størrelse er fragmenterne med den forudgående størrelse for et næste fragmenteringstrin; (d) gentagelse af trinene (b) og (c) efter behov, indtil det sidste niveau af blandinger er opnået; og hvor sekvensresultaterne opnås fra mindst en del af et eller flere fragmenter med den anden størrelse i hver blanding på det sidste niveau.
DK06760745.7T 2005-06-15 2006-06-13 Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter DK1907571T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US69077105P 2005-06-15 2005-06-15
US72511605P 2005-10-07 2005-10-07
US77641506P 2006-02-24 2006-02-24
PCT/US2006/022950 WO2006138284A2 (en) 2005-06-15 2006-06-13 Nucleic acid analysis by random mixtures of non-overlapping fragments

Publications (1)

Publication Number Publication Date
DK1907571T3 true DK1907571T3 (da) 2017-08-21

Family

ID=37571035

Family Applications (4)

Application Number Title Priority Date Filing Date
DK06760745.7T DK1907571T3 (da) 2005-06-15 2006-06-13 Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter
DK12150825.3T DK2463386T3 (da) 2005-06-15 2006-06-13 Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter
DK12180882.8T DK2620510T4 (da) 2005-06-15 2006-06-13 Enkeltmolekyle-arrays til genetisk og kemisk analyse
DK06784802.8T DK1907583T4 (da) 2005-06-15 2006-06-13 Enkeltmolekyle-arrays til genetisk og kemisk analyse

Family Applications After (3)

Application Number Title Priority Date Filing Date
DK12150825.3T DK2463386T3 (da) 2005-06-15 2006-06-13 Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter
DK12180882.8T DK2620510T4 (da) 2005-06-15 2006-06-13 Enkeltmolekyle-arrays til genetisk og kemisk analyse
DK06784802.8T DK1907583T4 (da) 2005-06-15 2006-06-13 Enkeltmolekyle-arrays til genetisk og kemisk analyse

Country Status (11)

Country Link
US (29) US7709197B2 (da)
EP (7) EP1907571B1 (da)
JP (4) JP5331476B2 (da)
CN (1) CN101466847B (da)
AU (1) AU2006259565B2 (da)
CA (2) CA2611743C (da)
DK (4) DK1907571T3 (da)
HK (1) HK1209795A1 (da)
IL (2) IL188142A (da)
SG (1) SG162795A1 (da)
WO (2) WO2006138257A2 (da)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11414702B2 (en) 2005-06-15 2022-08-16 Complete Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments

Families Citing this family (721)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
CN1791682B (zh) * 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
US8785353B2 (en) 2005-06-23 2014-07-22 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
US10316364B2 (en) * 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
EP1951900B1 (en) * 2005-10-07 2016-06-15 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
ES2882401T3 (es) 2005-12-22 2021-12-01 Keygene Nv Método de detección de polimorfismos basado en AFLP de alto rendimiento
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN1876840A (zh) * 2006-04-26 2006-12-13 东南大学 一种多拷贝的单分子核酸阵列芯片
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US7629125B2 (en) * 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US9874501B2 (en) 2006-11-24 2018-01-23 Curiox Biosystems Pte Ltd. Use of chemically patterned substrate for liquid handling, chemical and biological reactions
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US12497656B2 (en) * 2007-01-26 2025-12-16 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US20090053690A1 (en) 2007-02-02 2009-02-26 California Institute Of Technology Surface chemistry and deposition techniques
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
WO2008121384A1 (en) * 2007-03-30 2008-10-09 University Of Georgia Research Foundation, Inc. Source tagging and normalization of dna for parallel dna sequencing, and direct measurement of mutation rates using the same
CN101720359A (zh) * 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
DE102007034967A1 (de) 2007-07-26 2009-01-29 Plansee Se Brennstoffzelle und Verfahren zu deren Herstellung
WO2009135212A2 (en) 2008-05-02 2009-11-05 Epicentre Technologies Corporation Selective 5' ligation tagging of rna
US8759077B2 (en) * 2007-08-28 2014-06-24 Lightspeed Genomics, Inc. Apparatus for selective excitation of microparticles
US8222040B2 (en) * 2007-08-28 2012-07-17 Lightspeed Genomics, Inc. Nucleic acid sequencing by selective excitation of microparticles
US20090061424A1 (en) * 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
ITBO20070627A1 (it) * 2007-09-14 2009-03-15 Twof Inc Metodo per la preparazione di dna microarray con sonde ad alta densita' lineare
EP2203566A4 (en) * 2007-09-28 2011-02-09 Pacific Biosciences California ERROR-FREE DNA AMPLIFICATION FOR CLONAL SEQUENCING
EP2201136B1 (en) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Nanopore sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
CN105349647B (zh) 2007-10-30 2020-08-28 完整基因有限公司 用于核酸高通量测序的方法
US7988918B2 (en) * 2007-11-01 2011-08-02 Complete Genomics, Inc. Structures for enhanced detection of fluorescence
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2013114217A1 (en) 2012-02-05 2013-08-08 Curiox Biosystems Pte Ltd. Array plates and methods for making and using same
US10725020B2 (en) 2007-11-14 2020-07-28 Curiox Biosystems Pte Ltd. High throughput miniaturized assay system and methods
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
SG193776A1 (en) * 2007-12-05 2013-10-30 Complete Genomics Inc Efficient base determination in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN103290106B (zh) * 2007-12-05 2015-07-29 考利达基因组股份有限公司 测序反应中碱基的有效确定
US20090171640A1 (en) * 2007-12-28 2009-07-02 Microsoft Corporation Population sequencing using short read technologies
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
TWI460602B (zh) * 2008-05-16 2014-11-11 Counsyl Inc 廣用的懷孕前篩檢裝置
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8882980B2 (en) 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2664678B1 (en) 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2364368B1 (en) 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
ES2568509T3 (es) 2009-01-15 2016-04-29 Adaptive Biotechnologies Corporation Perfilado de la inmunidad adaptativa y métodos para la generación de anticuerpos monoclonales
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
US8738296B2 (en) * 2009-02-03 2014-05-27 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
EP2394165A4 (en) * 2009-02-03 2013-12-11 Complete Genomics Inc ASSIGNMENT OF OLIGOMER SEQUENCES
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2511843B1 (en) * 2009-04-29 2016-12-21 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
US20130296189A1 (en) * 2009-05-08 2013-11-07 Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences Probes utilizing universal tags, a kit comprising the same and detection methods
US8246799B2 (en) 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
KR20110018763A (ko) * 2009-08-18 2011-02-24 삼성전자주식회사 표적 물질을 기판에 고정하는 방법 및 장치
ES2562159T3 (es) 2009-08-20 2016-03-02 Population Genetics Technologies Ltd. Composiciones y métodos para el reordenamiento de ácido nucleico molecular
US10385391B2 (en) * 2009-09-22 2019-08-20 President And Fellows Of Harvard College Entangled mate sequencing
ES2640776T3 (es) 2009-09-30 2017-11-06 Natera, Inc. Métodos para denominar de forma no invasiva ploidía prenatal
JP5901530B2 (ja) * 2009-11-10 2016-04-13 ネステク ソシエテ アノニム 心臓加齢バイオマーカー及び使用方法
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
WO2011085075A2 (en) * 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2933629B1 (en) 2010-02-19 2019-04-10 Pacific Biosciences Of California, Inc. System for measuring analytical reactions comprising a socket for an optode array chip
CN202281746U (zh) 2010-03-06 2012-06-20 伊鲁米那股份有限公司 检测来自样品光信号的测定设备及其光学组件和光学系统
US8502867B2 (en) 2010-03-19 2013-08-06 Lightspeed Genomics, Inc. Synthetic aperture optics imaging method using minimum selective excitation patterns
US9465228B2 (en) 2010-03-19 2016-10-11 Optical Biosystems, Inc. Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
EP2854058A3 (en) 2010-05-18 2015-10-28 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US20240158855A1 (en) 2010-05-18 2024-05-16 Natera, Inc. Methods for simultaneous amplification of target loci
US12655483B2 (en) 2010-05-18 2026-06-16 Natera, Inc. Methods for simultaneous amplification of target loci
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US20120035062A1 (en) 2010-06-11 2012-02-09 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US20120001646A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
JP2013533482A (ja) 2010-06-30 2013-08-22 ライフ テクノロジーズ コーポレーション イオン感応性電荷蓄積回路および方法
US8432149B2 (en) 2010-06-30 2013-04-30 Life Technologies Corporation Array column integrator
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
CN103168341B (zh) 2010-07-03 2016-10-05 生命科技公司 具有轻度掺杂的排出装置的化学敏感的传感器
AU2011274642B2 (en) * 2010-07-09 2015-05-21 Cergentis B.V. 3-d genomic region of interest sequencing strategies
WO2012011877A2 (en) 2010-07-23 2012-01-26 Curiox Biosystems Pte Ltd Apparatus and method for multiple reactions in small volumes
KR20130098321A (ko) * 2010-08-20 2013-09-04 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 소분자 어레이 및 이의 제조 및 사용 방법
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
US9671344B2 (en) * 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
FR2964391B1 (fr) * 2010-09-03 2014-04-11 Centre Nat Rech Scient Biopuces pour l'analyse de la dynamique de molecules d'acide nucleique
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
CN103299182A (zh) 2010-09-24 2013-09-11 生命科技公司 匹配的晶体管对电路
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012067911A1 (en) 2010-11-16 2012-05-24 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
AU2011348100B2 (en) 2010-12-22 2016-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2658999B1 (en) 2010-12-30 2019-03-13 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CN103703143B (zh) 2011-01-31 2016-12-14 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2012109574A2 (en) 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN107368705B (zh) * 2011-04-14 2021-07-13 完整基因有限公司 分析生物体的基因组dna的方法和计算机系统
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
EP3624124B1 (en) 2011-08-18 2023-11-22 Life Technologies Corporation Systems for making base calls in nucleic acid sequencing
WO2013028739A1 (en) 2011-08-25 2013-02-28 Complete Genomics Phasing of heterozygous loci to determine genomic haplotypes
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
HK1199745A1 (en) * 2011-09-09 2015-07-17 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
HK1201565A1 (en) 2011-10-21 2015-09-04 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
CN103890161A (zh) * 2011-10-31 2014-06-25 株式会社日立高新技术 核酸扩增方法、核酸基板、核酸分析方法及核酸分析装置
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
EP3388535B1 (en) 2011-12-09 2021-03-24 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP2605001A1 (en) * 2011-12-15 2013-06-19 Hain Lifescience GmbH A device and method for optically measuring fluorescence of nucleic acids in test samples and use of the device and method
US9404147B2 (en) * 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
EP2794928B1 (en) 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
WO2013119845A1 (en) 2012-02-07 2013-08-15 Vibrant Holdings, Llc Substrates, peptide arrays, and methods
US20130217023A1 (en) * 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
LT2825672T (lt) * 2012-03-13 2019-06-10 Swift Biosciences, Inc. Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
JP2015511015A (ja) * 2012-03-16 2015-04-13 ライフ テクノロジーズ コーポレーション 生体試料を収容するためのシステム及び方法
US9803239B2 (en) * 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
JP6159391B2 (ja) 2012-04-03 2017-07-05 イラミーナ インコーポレーテッド 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
WO2013166517A1 (en) 2012-05-04 2013-11-07 Complete Genomics, Inc. Methods for determining absolute genome-wide copy number variations of complex tumors
PT2831276T (pt) 2012-05-08 2016-07-26 Adaptive Biotechnologies Corp Composições e método para medição e calibração do viés de amplificação em reações pcr multiplex
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US9052963B2 (en) 2012-05-21 2015-06-09 International Business Machines Corporation Cloud computing data center machine monitor and control
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014008635A1 (zh) * 2012-07-11 2014-01-16 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
EP2885626B1 (en) 2012-08-20 2020-03-04 Illumina, Inc. An integrated detection, flow cell and photonics (dfp) device
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
CA3088591C (en) * 2012-09-28 2023-01-03 Vibrant Holdings, Llc Inverted pillar plates for assaying microarrays and methods of using same.
EP2904111B1 (en) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
US10286376B2 (en) 2012-11-14 2019-05-14 Vibrant Holdings, Llc Substrates, systems, and methods for array synthesis and biomolecular analysis
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
JP6395718B2 (ja) 2012-11-19 2018-09-26 アプトン バイオシステムズ インコーポレイテッド 単一分子検出を用いた分子分析物のデジタル分析の方法
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) * 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN105190656B (zh) 2013-01-17 2018-01-16 佩索纳里斯公司 用于遗传分析的方法和系统
EP2956550B1 (en) 2013-01-18 2020-04-08 Nabsys 2.0 LLC Enhanced probe binding
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
CN108624668B (zh) 2013-02-01 2022-12-02 加利福尼亚大学董事会 用于基因组组装及单体型定相的方法
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
KR20150115013A (ko) * 2013-02-07 2015-10-13 코닌클리케 필립스 엔.브이. 뉴클레오티드 시퀀스들의 처리
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
EP2956771B1 (en) 2013-02-15 2020-04-08 Vibrant Holdings, LLC Methods and compositions for amplified electrochemiluminescence detection
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US9890425B2 (en) 2013-03-15 2018-02-13 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
CA2906218A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105283758B (zh) 2013-03-15 2018-06-05 生命科技公司 具有一致传感器表面区域的化学传感器
EP2972281B1 (en) 2013-03-15 2023-07-26 Life Technologies Corporation Chemical device with thin conductive element
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
SG10201708498VA (en) 2013-04-17 2017-11-29 Agency Science Tech & Res Method for generating extended sequence reads
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
EP3603679B1 (en) 2013-06-04 2022-08-10 President and Fellows of Harvard College Rna-guided transcriptional regulation
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
DK3431614T3 (da) 2013-07-01 2021-12-06 Illumina Inc Katalysator fri overfladefunktionalisering og polymerpodning
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN105358715B (zh) 2013-07-03 2018-09-18 伊鲁米那股份有限公司 正交合成测序
US9557318B2 (en) 2013-07-09 2017-01-31 Curiox Biosystems Pte Ltd. Array plates for washing samples
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CN113789372B (zh) 2013-08-08 2025-02-25 伊鲁米那股份有限公司 用于向流动池的试剂输送的流体系统
CA2921809A1 (en) 2013-08-22 2015-02-26 Apton Biosystems, Inc. Digital analysis of molecular analytes using electrical methods
CN105637099B (zh) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 使用短读段的长片段从头组装
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
EP3038834B1 (en) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
JP6532456B2 (ja) 2013-10-04 2019-06-19 ライフ テクノロジーズ コーポレーション 終止化学を用いる配列決定における整相効果（ｐｈａｓｉｎｇ ｅｆｆｅｃｔｓ）をモデル化するための方法及びシステム
ES2885191T3 (es) 2013-10-17 2021-12-13 Illumina Inc Métodos y composiciones para preparar bibliotecas de ácidos nucleicos
EP3080268A1 (en) 2013-12-09 2016-10-19 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
EP4220137A1 (en) 2013-12-10 2023-08-02 Illumina, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
IL274464B2 (en) 2013-12-11 2023-08-01 Accuragen Holdings Ltd Preparations and methods for the detection of rare sequence variants
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
JP2015139373A (ja) * 2014-01-27 2015-08-03 株式会社日立ハイテクノロジーズ 生体分子分析デバイス、及び生体分子分析装置
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
EP3122894A4 (en) * 2014-03-28 2017-11-08 GE Healthcare Bio-Sciences Corp. Accurate detection of rare genetic variants in next generation sequencing
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
RU2717641C2 (ru) 2014-04-21 2020-03-24 Натера, Инк. Обнаружение мутаций и плоидности в хромосомных сегментах
US9751668B2 (en) 2014-05-02 2017-09-05 Synthetic Genomics, Inc. Tamper-resistant assembly for securing valuable material
AU2015259024B2 (en) 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US10829814B2 (en) 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4235677A3 (en) 2014-06-26 2023-11-22 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US10605766B2 (en) 2014-07-15 2020-03-31 Illumina, Inc. Biochemically activated electronic device
US20160032281A1 (en) * 2014-07-31 2016-02-04 Fei Company Functionalized grids for locating and imaging biological specimens and methods of using the same
AU2015296029B2 (en) 2014-08-01 2022-01-27 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
US10570385B2 (en) 2014-08-26 2020-02-25 Japan Science And Technology Agency Method for non-enzymatic combination of nucleic acid chains
KR102538753B1 (ko) 2014-09-18 2023-05-31 일루미나, 인코포레이티드 핵산 서열결정 데이터를 분석하기 위한 방법 및 시스템
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
PT3212684T (pt) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Revestimentos de polímeros e de copolímero de dna
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
DK3215616T3 (da) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
CN107124888B (zh) * 2014-11-21 2021-08-06 深圳华大智造科技股份有限公司 鼓泡状接头元件和使用其构建测序文库的方法
EP3221695B1 (en) 2014-11-21 2023-09-27 The Research Institute at Nationwide Children's Hospital Parallel-processing systems and methods for highly scalable analysis of biological sequence data
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
EP3498866B1 (en) 2014-11-25 2026-02-25 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
PT3234187T (pt) * 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
CN107407656B (zh) 2014-12-18 2020-04-07 生命科技公司 使用大规模 fet 阵列测量分析物的方法和装置
TWI756167B (zh) 2014-12-18 2022-03-01 美商生命技術公司 積體電路裝置、感測器裝置及積體電路
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CA2968417A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
DE112015005747B4 (de) * 2015-02-02 2023-01-26 Hitachi High-Tech Corporation Mehrfarb-Fluoreszenz-Analysevorrichtung
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
SG11201705996PA (en) 2015-02-09 2017-09-28 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data
JP6777966B2 (ja) 2015-02-17 2020-10-28 ダブテイル ゲノミクス エルエルシー 核酸配列アセンブリ
WO2016133764A1 (en) * 2015-02-17 2016-08-25 Complete Genomics, Inc. Dna sequencing using controlled strand displacement
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN114250278B (zh) 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
CA2979850C (en) 2015-03-24 2020-07-21 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
CN107810415B (zh) 2015-05-11 2021-06-22 亿明达股份有限公司 用于发现和分析治疗剂的平台
EP4220645A3 (en) 2015-05-14 2023-11-08 Life Technologies Corporation Barcode sequences, and related systems and methods
KR102134953B1 (ko) 2015-05-29 2020-07-16 일루미나, 인코포레이티드 지정된 반응을 수행하기 위한 샘플 캐리어 및 검정 시스템
US10545139B2 (en) 2015-06-16 2020-01-28 Curiox Biosystems Pte Ltd. Methods and devices for performing biological assays using magnetic components
HK1253950A1 (zh) 2015-07-07 2019-07-05 Illumina, Inc. 经由纳米压印的选择性表面图案化
EP3325648B1 (en) 2015-07-17 2023-03-29 Illumina, Inc. Polymer sheets for sequencing applications
IL294145B2 (en) 2015-07-30 2024-02-01 Illumina Inc Removal of orthogonal blocking of nucleotides
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
WO2017037078A1 (en) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CN114807323A (zh) 2015-10-09 2022-07-29 安可济控股有限公司 用于富集扩增产物的方法及组合物
SG11201803289VA (en) 2015-10-19 2018-05-30 Dovetail Genomics Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
CN108474022A (zh) 2015-11-03 2018-08-31 哈佛学院董事及会员团体 用于包含三维核酸的基质容积成像的设备和方法
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
EP3383994A4 (en) * 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
JP2019508669A (ja) 2016-01-11 2019-03-28 イラミーナ インコーポレーテッド マイクロフルオロメータ、流体システム、およびフローセルラッチクランプモジュールを有する検出装置
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US10975417B2 (en) 2016-02-23 2021-04-13 Dovetail Genomics, Llc Generation of phased read-sets for genome assembly and haplotype phasing
JP6584986B2 (ja) 2016-03-18 2019-10-02 株式会社東芝 核酸検出方法
JP7280044B2 (ja) 2016-04-15 2023-05-23 ナテラ， インコーポレイテッド 肺癌の検出方法
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017197300A1 (en) 2016-05-13 2017-11-16 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
CN109313396B (zh) 2016-05-18 2022-11-22 Illumina公司 使用图案化疏水性表面进行的自组装图案化
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
CN109643578B (zh) 2016-06-01 2023-07-21 生命科技股份有限公司 用于设计基因组合的方法和系统
JP6889769B2 (ja) * 2016-07-18 2021-06-18 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 核酸配列決定の非対称な鋳型および非対称な方法
WO2018035170A1 (en) * 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
EP4582805A3 (en) 2016-08-31 2025-10-08 President And Fellows Of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
CN111781139B (zh) 2016-10-14 2023-09-12 亿明达股份有限公司 夹盒组件
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
BR112019008939B1 (pt) 2016-11-03 2023-11-07 Mgi Tech Co., Ltd Biossensor, método, e, método para sequenciamento de dna
CA3044231A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
FI4112741T3 (fi) 2017-01-04 2025-05-15 Mgi Tech Co Ltd Vaiheittainen sekvensointi ei-leimatuilla, reversiibeleillä terminaattoreilla tai luonnollisilla nukleotideilla
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN110382710B (zh) 2017-01-24 2024-10-01 瓦斯特根公司 构建核酸分子拷贝的方法
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
WO2018145041A1 (en) 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3595806A4 (en) * 2017-03-17 2021-06-16 Apton Biosystems, Inc. SEQUENCING AND HIGH RESOLUTION IMAGING
KR102535751B1 (ko) 2017-03-20 2023-05-22 엠쥐아이 테크 컴퍼니 엘티디. 생물학적 또는 화학적 분석을 위한 바이오센서 및 그 제조 방법
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
EP4280260A3 (en) 2017-04-05 2024-03-06 Curiox Biosystems Pte Ltd. Methods, devices, and apparatus for washing samples on array plates
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2018218250A2 (en) 2017-05-26 2018-11-29 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP6991504B2 (ja) * 2017-07-13 2022-01-12 国立研究開発法人産業技術総合研究所 目的物質検出装置及び目的物質検出方法
WO2019023948A1 (zh) 2017-08-01 2019-02-07 深圳华大智造科技有限公司 基因测序反应设备、基因测序系统和基因测序反应方法
JP2021500531A (ja) 2017-08-15 2021-01-07 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 化学的および生物学的検体の検出に有用なスキャン装置および方法
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
US11125748B2 (en) 2017-09-01 2021-09-21 University Of British Columbia Method for organizing individual molecules on a patterned substrate and structures assembled thereby
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
WO2019060177A1 (en) 2017-09-19 2019-03-28 Complete Genomics, Inc. MANUFACTURE OF SEQUENCING FLOW CELLS AT THE WAFER
DK3684949T3 (en) 2017-09-22 2024-07-08 Univ Washington In situ combinatorial labeling of cellular molecules
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3696275B1 (en) 2017-10-11 2025-12-24 MGI Tech Co., Ltd. Method for improving loading and stability of nucleic acid on solid support
EP3622519B1 (en) 2017-10-16 2023-09-13 Illumina, Inc. Deep learning-based aberrant splicing detection
US11315016B2 (en) 2017-10-16 2022-04-26 Illumina, Inc. Deep convolutional neural networks for variant classification
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
KR102370055B1 (ko) 2018-01-08 2022-03-03 일루미나, 인코포레이티드 반도체-기반 검출을 사용하는 고-처리율 서열분석용 시스템 및 디바이스
EP3737773B1 (en) 2018-01-12 2023-04-12 Natera, Inc. Novel primers and uses thereof
WO2019140402A1 (en) 2018-01-15 2019-07-18 Illumina, Inc. Deep learning-based variant classifier
WO2019152539A1 (en) 2018-01-30 2019-08-08 Optical Biosystems, Inc. Method for detecting particles using structured illumination
WO2019152543A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
EP3775272B1 (en) * 2018-04-02 2025-07-30 Enumera Molecular, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CN112236218B (zh) 2018-04-02 2022-04-26 滴管公司 用于连续流乳液处理的系统和方法
CN112236528A (zh) 2018-04-04 2021-01-15 诺迪勒思生物科技公司 产生纳米阵列和微阵列的方法
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019200338A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant classifier based on deep neural networks
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
DK3781714T3 (da) 2018-04-14 2026-03-30 Natera Inc Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna
CN108444966A (zh) * 2018-04-16 2018-08-24 成都博奥晶芯生物科技有限公司 一种激光微阵列芯片扫描仪的浓度梯度荧光校准片
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP7554117B2 (ja) 2018-04-26 2024-09-19 パシフィック・バイオサイエンシズ・オブ・カリフォルニア・インク． 核酸－ヌクレオチド－ポリメラーゼ複合体を安定化するための方法及び組成物
ES2947437T3 (es) 2018-05-08 2023-08-09 Mgi Tech Co Ltd Creación de Códigos de barra compartidos en el ADN, en un solo tubo con perlas, para la secuenciación, haplotipado y ensamblaje preciso y rentable
EP3790985B1 (en) 2018-05-09 2026-04-15 Vibrant Holdings, LLC Methods of synthesizing a polynucleotide array using photactivated agents
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
RU2744175C1 (ru) 2018-05-17 2021-03-03 Иллумина, Инк. Высокопроизводительное секвенирование одиночной клетки со сниженной ошибкой амплификации
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
JP2021525078A (ja) 2018-05-31 2021-09-24 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸配列決定におけるシグナル対ノイズの増加
CA3253118A1 (en) 2018-06-04 2026-03-02 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
WO2019236749A2 (en) 2018-06-06 2019-12-12 Ignite Biosciences, Inc. Methods and applications of protein identification
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN112601823A (zh) 2018-06-12 2021-04-02 安可济控股有限公司 用于形成连接产物的方法和组合物
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
JP2020000197A (ja) * 2018-06-29 2020-01-09 キヤノン株式会社 計数方法、濃度測定装置及び濃度測定システム
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
SG11202101405RA (en) 2018-08-14 2021-03-30 Harvard College In vitro detection of nucleic acid
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020061237A1 (en) * 2018-09-19 2020-03-26 Apton Biosystems, Inc. Densely-packed analyte layers and detection methods
EP3626344A1 (de) * 2018-09-21 2020-03-25 Eppendorf AG Verfahren zur steuerung eines thermocyclers und thermocycler
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN113705585A (zh) 2018-10-15 2021-11-26 因美纳有限公司 基于神经网络实现的方法和系统
CN120082639A (zh) 2018-11-09 2025-06-03 深圳华大智造科技股份有限公司 使用未标记的核苷酸进行大规模平行测序
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US20200149095A1 (en) * 2018-11-14 2020-05-14 Element Biosciences, Inc. Low binding supports for improved solid-phase dna hybridization and amplification
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
GB2613480B (en) * 2018-11-15 2023-11-22 Element Biosciences Inc Methods for generating circular nucleic acid molecules
TWI853851B (zh) 2018-11-15 2024-09-01 中國商深圳華大智造科技有限公司 微流體裝置與其製備方法
JP2022513092A (ja) 2018-11-20 2022-02-07 ノーティラス・バイオテクノロジー・インコーポレイテッド 親和性試薬の設計および選択
WO2020108588A1 (en) 2018-12-01 2020-06-04 Mgi Tech Co., Ltd. Methods and structures to improve light collection efficiency in biosensors
CN211311438U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 流动池
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
EP3918088B1 (en) 2019-01-29 2024-03-13 MGI Tech Co., Ltd. High coverage stlfr
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US11680950B2 (en) 2019-02-20 2023-06-20 Pacific Biosciences Of California, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
WO2020180778A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
WO2020180813A1 (en) 2019-03-06 2020-09-10 Qiagen Sciences, Llc Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020205296A1 (en) 2019-03-21 2020-10-08 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
SG11202102801TA (en) 2019-04-29 2021-04-29 Illumina Inc Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
AU2020266136B2 (en) 2019-04-29 2026-04-02 Nautilus Subsidiary, Inc. Methods and systems for integrated on-chip single-molecule detection
CN114286861B (zh) * 2019-05-13 2024-12-06 Lgc基因组学有限责任公司 捕获和分析靶基因组区域
CA3104851A1 (en) 2019-05-16 2020-11-19 Illumina, Inc. Base calling using convolutions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11841374B2 (en) * 2019-06-27 2023-12-12 Mjnn Llc Laboratory automation system implementing efficient path for material and lab ware transfers
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021050717A1 (en) * 2019-09-10 2021-03-18 The Regents Of The University Of California Immune cell sequencing methods
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
JP7470787B2 (ja) 2019-11-05 2024-04-18 パーソナリス，インコーポレイティド 単一試料からの腫瘍純度の推定
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
WO2021119201A1 (en) 2019-12-10 2021-06-17 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
WO2021127436A2 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US12448648B2 (en) 2019-12-23 2025-10-21 Mgi Tech Co., Ltd. Controlled strand-displacement for paired end sequencing
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4104179B1 (en) 2020-02-13 2026-01-28 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12591780B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Data compression for artificial intelligence-based base calling
US12592298B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Hardware execution and acceleration of artificial intelligence-based base caller
JP7747643B2 (ja) 2020-02-20 2025-10-01 イルミナ インコーポレイテッド 人工知能ベースの多対多ベースコール
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4114966B1 (en) 2020-03-03 2025-02-26 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021188717A1 (en) * 2020-03-18 2021-09-23 Apton Biosystems, Inc. Systems and methods of detecting densely-packed analytes
CN120502365A (zh) * 2020-04-21 2025-08-19 赫孚孟拉罗股份公司 具有单分子传感器阵列的高通量核酸定序
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
AU2021275128A1 (en) * 2020-05-20 2023-01-19 Element Biosciences, Inc. Methods for paired-end sequencing library preparation
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252375A1 (en) 2020-06-08 2021-12-16 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
KR20230020977A (ko) 2020-06-09 2023-02-13 일루미나, 인코포레이티드 시퀀싱 라이브러리의 생성을 증가시키는 방법
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4164796A4 (en) 2020-06-10 2024-03-06 10x Genomics, Inc. Fluid delivery methods
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
GB202010691D0 (en) * 2020-07-10 2020-08-26 Vidya Holdings Ltd Improvements in or relating to an apparatus for imaging
US20240043913A1 (en) 2020-07-17 2024-02-08 The Regents Of The University Of Michigan Materials and methods for localized detection of nucleic acids in a tissue sample
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11200446B1 (en) 2020-08-31 2021-12-14 Element Biosciences, Inc. Single-pass primary analysis
US12505571B2 (en) 2020-08-31 2025-12-23 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
US12469162B2 (en) 2020-08-31 2025-11-11 Element Biosciences, Inc. Primary analysis in next generation sequencing
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12655450B1 (en) 2020-10-27 2026-06-16 10X Genomics, Inc. Methods, systems, and kits for detection of nucleic acid molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
BE1028339B1 (fr) * 2020-11-09 2021-12-21 A Mansia Biotech Composition comprenant de l'Akkermansia muciniphila et de l'extrait de thé vert
EP4244382A1 (en) 2020-11-11 2023-09-20 Nautilus Subsidiary, Inc. Affinity reagents having enhanced binding and detection characteristics
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12576399B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for imaging and analyzing a cell in a compartment of a fluidic device
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
EP4277740A1 (en) * 2021-01-13 2023-11-22 Pacific Biosciences of California, Inc. Surface structuring with colloidal assembly
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US12540948B2 (en) 2021-03-05 2026-02-03 Countable Labs, Inc. Systems and methods for generating droplets and performing digital analyses
WO2022187684A1 (en) 2021-03-05 2022-09-09 Enumerix, Inc. Systems and methods for generating droplets and performing digital analyses
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
US20220301657A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
EP4524261B1 (en) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12176070B2 (en) * 2021-04-19 2024-12-24 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4352286A4 (en) * 2021-06-11 2025-04-23 Cellanome, Inc. Systems and methods for analyzing biological samples
US12570831B2 (en) 2021-06-15 2026-03-10 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
EP4679300A3 (en) 2021-07-19 2026-04-22 Illumina, Inc. Intensity extraction with interpolation and adaptation for base calling
EP4373958A1 (en) 2021-07-23 2024-05-29 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
EP4381095A1 (en) * 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023091592A1 (en) * 2021-11-19 2023-05-25 Dovetail Genomics, Llc Dendrimers for genomic analysis methods and compositions
EP4441503A1 (en) 2021-11-30 2024-10-09 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
AU2022407175A1 (en) * 2021-12-10 2024-06-27 Element Biosciences, Inc. Primary analysis in next generation sequencing
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
US12661647B2 (en) 2022-03-22 2026-06-23 Cellanome, Inc. Methods for enclosing and analyzing a cell in a fluidic device
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
EP4551718B1 (en) 2022-07-05 2026-06-24 Element Biosciences, Inc. Pcr-free library preparation using double-stranded splint adaptors and methods of use
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US20240053333A1 (en) 2022-08-03 2024-02-15 Nautilus Subsidiary, Inc. Chemical modification of antibodies and functional fragments thereof
AU2023325054A1 (en) 2022-08-15 2025-02-27 Element Biosciences, Inc. Methods for preparing nucleic acid nanostructures using compaction oligonucleotides
CA3264896A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS
EP4587566A1 (en) 2022-09-12 2025-07-23 Element Biosciences, Inc. Double-stranded splint adaptors with universal long splint strands and methods of use
EP4594519A1 (en) 2022-09-27 2025-08-06 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
EP4594481A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Helicase-cytidine deaminase complexes and methods of use
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
CN115409174B (zh) * 2022-11-01 2023-03-31 之江实验室 一种基于dram存内计算的碱基序列过滤方法与装置
EP4685239A1 (en) 2022-11-09 2026-01-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
US20240417720A1 (en) 2023-06-14 2024-12-19 Nautilus Subsidiary, Inc. Making and using structured nucleic acid particles
US20240426839A1 (en) 2023-06-21 2024-12-26 Nautilus Subsidiary, Inc. Compositions and methods for improving affinity reagent avidity
EP4739790A1 (en) 2023-07-06 2026-05-13 Pacific Biosciences of California, Inc. Methods and compositions for stabilizing concatemers
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
EP4784740A1 (en) 2023-09-28 2026-08-05 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
US20250306015A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Single molecule-resolved characterization of affinity reagent kinetics and thermodynamics
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
US12467087B1 (en) 2024-06-25 2025-11-11 Guardant Health, Inc. Sequencing methods with partitioning
WO2026006467A1 (en) 2024-06-27 2026-01-02 Nautilus Subsidiary, Inc. Enrichment of particle reagents for array-based assays
WO2026006629A1 (en) 2024-06-27 2026-01-02 Personalis, Inc. System and method for management of compressed sequencing files
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
US20260092914A1 (en) 2024-09-27 2026-04-02 Nautilus Subsidiary, Inc. Single analyte characterization under equilibrium binding conditions
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026128365A1 (en) 2024-12-09 2026-06-18 Illumina, Inc. Methods for increasing sequencing quality
US20260193640A1 (en) 2024-12-11 2026-07-09 Nautilus Subsidiary, Inc. Screening and characterization of binding reagents under equilibrium conditions

Family Cites Families (440)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3356882A (en) 1965-10-21 1967-12-05 Ford Motor Co Spark plug having the center electrode sheath with a nickel alloy
US3958144A (en) 1973-10-01 1976-05-18 Franks Harry E Spark plug
JPS5180845A (en) 1975-01-14 1976-07-15 Teijin Ltd 2*22 bisu * 3*55 jiburomu 44 * 22 hidorokishetokishi * fueniru * puropannoyojuhojiho
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
JPS5965108A (ja) 1982-10-06 1984-04-13 Sumikin Kozai Kogyo Kk 鋼製合掌堰堤とその構築方法
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4605735A (en) 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US4719179A (en) 1984-11-30 1988-01-12 Pharmacia P-L Biochemicals, Inc. Six base oligonucleotide linkers and methods for their use
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4925785A (en) 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
US5091519A (en) 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4725254A (en) 1986-11-24 1988-02-16 Allied Corporation Method for manufacturing a center electrode for a spark plug
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
DE3813278A1 (de) 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum nachweis von nukleinsaeuren
US5354657A (en) 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5066580A (en) 1988-08-31 1991-11-19 Becton Dickinson And Company Xanthene dyes that emit to the red of fluorescein
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5194599A (en) 1988-09-23 1993-03-16 Gilead Sciences, Inc. Hydrogen phosphonodithioate compositions
DE3836656A1 (de) 1988-10-27 1990-05-03 Boehringer Mannheim Gmbh Neue digoxigenin-derivate und ihre verwendung
US5091302A (en) 1989-04-27 1992-02-25 The Blood Center Of Southeastern Wisconsin, Inc. Polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic applications thereof
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
DE3924454A1 (de) 1989-07-24 1991-02-07 Cornelis P Prof Dr Hollenberg Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips)
JPH03101086A (ja) 1989-09-14 1991-04-25 Ngk Spark Plug Co Ltd 内燃機関用スパークプラグ
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5003097A (en) 1989-10-02 1991-03-26 The United States Of America As Represented By The Department Of Health And Human Services Method for the sulfurization of phosphorous groups in compounds
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5166387A (en) 1990-01-12 1992-11-24 Applied Biosystems, Inc. Method of synthesizing sulfurized oligonucleotide analogs with thiuram disulfides
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5151510A (en) 1990-04-20 1992-09-29 Applied Biosystems, Inc. Method of synethesizing sulfurized oligonucleotide analogs
GB9009980D0 (en) 1990-05-03 1990-06-27 Amersham Int Plc Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
US5073562A (en) 1990-05-10 1991-12-17 G. D. Searle & Co. Alkoxy-substituted dihydrobenzopyran-2-carboxylic acids and derivatives thereof
US5091320A (en) * 1990-06-15 1992-02-25 Bell Communications Research, Inc. Ellipsometric control of material growth
AU649066B2 (en) 1990-07-25 1994-05-12 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3080178B2 (ja) 1991-02-18 2000-08-21 東洋紡績株式会社 核酸配列の増幅方法およびそのための試薬キット
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
JP3085409B2 (ja) 1991-03-29 2000-09-11 東洋紡績株式会社 標的核酸配列の検出方法およびそのための試薬キット
US6589726B1 (en) 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
EP0604552B1 (en) 1991-09-18 1997-02-12 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
ZA927323B (en) 1991-09-24 1993-08-30 Keygene Nv Selective restriction fragment amplification: A general method for DNA fingerprinting
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
DE69233087T2 (de) 1991-11-22 2003-12-24 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Verfahren zur Herstellung von Polymerarrays
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
EP1382386A3 (en) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
GB9214873D0 (en) 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5583211A (en) 1992-10-29 1996-12-10 Beckman Instruments, Inc. Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
ES2204913T3 (es) 1993-04-12 2004-05-01 Northwestern University Metodo para formacion de oligonucleotidos.
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US6096880A (en) 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
WO1995011995A1 (en) 1993-10-26 1995-05-04 Affymax Technologies N.V. Arrays of nucleic acid probes on biological chips
EP0706531A4 (en) 1993-07-02 1998-11-25 Lynx Therapeutics Inc SYNTHESIS OF BRANCHED NUCLEIC ACIDS
US5473060A (en) 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications
NZ275194A (en) 1993-09-27 1997-09-22 Arch Dev Corp Method and kit for nucleic acid sequencing
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5472672A (en) 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
GB2285942A (en) 1994-01-25 1995-08-02 Ford Motor Co Forming an erosion resistant coating on an electrode
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
AU2360195A (en) 1994-05-05 1995-11-29 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US7625697B2 (en) 1994-06-17 2009-12-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for constructing subarrays and subarrays made thereby
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5744367A (en) 1994-11-10 1998-04-28 Igen International, Inc. Magnetic particle based electrochemiluminescent detection apparatus and method
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5866337A (en) 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
WO1996035940A1 (en) 1995-05-12 1996-11-14 Novartis Ag Sensor platform and method for the parallel detection of a plurality of analytes using evanescently excited luminescence
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
US5774305A (en) 1995-06-07 1998-06-30 Seagate Technology, Inc. Head gimbal assembly to reduce slider distortion due to thermal stress
US5675209A (en) 1995-06-19 1997-10-07 Hoskins Manufacturing Company Electrode material for a spark plug
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US6132580A (en) 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
ATE496288T1 (de) 1995-10-11 2011-02-15 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5658734A (en) 1995-10-17 1997-08-19 International Business Machines Corporation Process for synthesizing chemical compounds
DK0862656T3 (da) 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DE69636080T2 (de) 1995-12-05 2006-11-30 Erland Jorn Koch Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6660233B1 (en) 1996-01-16 2003-12-09 Beckman Coulter, Inc. Analytical biochemistry system with robotically carried bioarray
ATE305052T1 (de) * 1996-03-01 2005-10-15 Univ Dundee Drogen untersuchungssystem
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
EP0902885A4 (en) 1996-05-16 2006-09-27 Affymetrix Inc SYSTEMS AND METHODS FOR DETECTION OF BRANDED PRODUCTS
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5869245A (en) 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
EP0925494B1 (en) 1996-09-04 2001-12-19 Scandinavian Micro Biodevices A/S A micro flow system for particle separation and analysis
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
CA2702219C (en) 1996-11-06 2013-01-08 Sequenom, Inc. High density immobilization of nucleic acids
AU761161B2 (en) 1996-11-06 2003-05-29 Sequenom, Inc. High density immobilization of nucleic acids
WO1998020967A1 (en) 1996-11-14 1998-05-22 Affymetrix, Inc. Chemical amplification for the synthesis of patterned arrays
US6054100A (en) * 1996-11-18 2000-04-25 Robbins Scientific Corporation Apparatus for multi-well microscale synthesis
US5916750A (en) 1997-01-08 1999-06-29 Biogenex Laboratories Multifunctional linking reagents for synthesis of branched oligomers
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US20020042048A1 (en) 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
US5994068A (en) 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
EP2327797B1 (en) † 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US20040229221A1 (en) 1997-05-08 2004-11-18 Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
US5919626A (en) † 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US5866473A (en) * 1997-10-31 1999-02-02 Advanced Micro Devices, Inc. Method of manufacturing a polysilicon gate having a dimension below the photolithography limitation
JP2001521753A (ja) 1997-10-31 2001-11-13 アフィメトリックス インコーポレイテッド 成人臓器及び胎児臓器中の発現プロフィール
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US20030039967A1 (en) * 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
US6087102A (en) 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
WO1999039817A1 (en) 1998-02-06 1999-08-12 Affymetrix, Inc. Method of quality control in manufacturing processes
US6136537A (en) 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US6174687B1 (en) 1999-02-26 2001-01-16 The Burnham Institute Methods of identifying lung homing molecules using membrane dipeptidase
DE19812729A1 (de) 1998-03-24 1999-09-30 Bosch Gmbh Robert Elektromotor, insbesondere mit einem Lüfterrad zur Bildung eines Axial- oder Radiallüfters
WO1999049079A1 (en) 1998-03-25 1999-09-30 Ulf Landegren Rolling circle replication of padlock probes
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6004755A (en) 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
US6284497B1 (en) * 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
JP4262799B2 (ja) 1998-04-16 2009-05-13 平田機工株式会社 生タイヤ供給方法
US6355419B1 (en) * 1998-04-27 2002-03-12 Hyseq, Inc. Preparation of pools of nucleic acids based on representation in a sample
US6270831B2 (en) 1998-04-30 2001-08-07 Medquest Products, Inc. Method and apparatus for providing a conductive, amorphous non-stick coating
US6255469B1 (en) 1998-05-06 2001-07-03 New York University Periodic two and three dimensional nucleic acid structures
US6031078A (en) 1998-06-16 2000-02-29 Millennium Pharmaceuticals, Inc. MTbx protein and nucleic acid molecules and uses therefor
US5980345A (en) 1998-07-13 1999-11-09 Alliedsignal Inc. Spark plug electrode having iridium based sphere and method for manufacturing same
WO2000004193A1 (en) * 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU770831B2 (en) † 1998-07-30 2004-03-04 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US6265021B1 (en) 1998-07-31 2001-07-24 International Business Machines Corporation Nanoparticle structures utilizing synthetic DNA lattices
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
US6046039A (en) 1998-08-19 2000-04-04 Battelle Memorial Institute Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
WO2000015779A2 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
US6426513B1 (en) 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6326144B1 (en) 1998-09-18 2001-12-04 Massachusetts Institute Of Technology Biological applications of quantum dots
US20020019007A1 (en) * 1998-09-18 2002-02-14 Jensen Wayne A. PCR methods and materials
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US7272507B2 (en) * 1999-10-26 2007-09-18 Michael Paul Strathmann Applications of parallel genomic analysis
EP1144684B1 (en) 1999-01-06 2009-08-19 Callida Genomics, Inc. Enhanced sequencing by hybridization using pools of probes
CA2356697C (en) 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6514768B1 (en) 1999-01-29 2003-02-04 Surmodics, Inc. Replicable probe array
EP1147229A2 (en) * 1999-02-02 2001-10-24 Bernhard O. Palsson Methods for identifying drug targets based on genomic sequence data
EP1196554B1 (en) 1999-03-18 2009-02-25 Complete Genomics AS Methods of cloning and producing fragment chains with readable information content
JP2003527075A (ja) 1999-03-25 2003-09-16 ハイセック，インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
CA2366459A1 (en) 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
DE60040741D1 (de) 1999-04-20 2008-12-18 Illumina Inc Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
WO2000068692A1 (en) 1999-05-07 2000-11-16 Quantum Dot Corporation A method of detecting an analyte using semiconductor nanocrystals
CA2374596A1 (en) 1999-05-20 2000-11-30 Illumina, Inc. Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
AU7569600A (en) 1999-05-20 2000-12-28 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US6573369B2 (en) 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US6326719B1 (en) 1999-06-16 2001-12-04 Alliedsignal Inc. Spark plug shell having a bimetallic ground electrode spark plug incorporating the shell, and method of making same
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
AU6387000A (en) 1999-07-29 2001-02-19 Genzyme Corporation Serial analysis of genetic alterations
US6440706B1 (en) † 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
AU1075701A (en) * 1999-10-08 2001-04-23 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6287778B1 (en) 1999-10-19 2001-09-11 Affymetrix, Inc. Allele detection using primer extension with sequence-coded identity tags
WO2001032935A2 (en) 1999-11-02 2001-05-10 Celine Hu Molecular microarrays and methods for production and use thereof
JP2003515149A (ja) 1999-11-26 2003-04-22 キュラジェン コーポレイション 核酸プローブアレイ
EP1238105A2 (en) 1999-12-02 2002-09-11 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
AU3015801A (en) 1999-12-23 2001-07-09 Axaron Bioscience Ag Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
AU2282801A (en) 1999-12-29 2001-07-09 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
JP2003521252A (ja) 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2001062982A2 (en) 2000-02-25 2001-08-30 Mosaic Technologies, Inc. Methods for multi-stage solid phase amplification of nucleic acids
US20020004204A1 (en) 2000-02-29 2002-01-10 O'keefe Matthew T. Microarray substrate with integrated photodetector and methods of use thereof
US6413722B1 (en) 2000-03-22 2002-07-02 Incyte Genomics, Inc. Polymer coated surfaces for microarray applications
DK2278029T3 (da) * 2000-04-10 2016-10-03 Taxon Biosciences Inc Fremgangsmåder til undersøgelse og genetisk analyse af populationer
US20020028455A1 (en) 2000-05-03 2002-03-07 Laibinis Paul E. Methods and reagents for assembling molecules on solid supports
EP1290225A4 (en) 2000-05-20 2004-09-15 Univ Michigan METHOD FOR PRODUCING A DNA BANK BY POSITIONAL REPRODUCTION
JP4304900B2 (ja) 2000-05-25 2009-07-29 富士ゼロックス株式会社 画像形成方法
DE10027651C2 (de) 2000-06-03 2002-11-28 Bosch Gmbh Robert Elektrode, Verfahren zu deren Herstellung und Zündkerze mit einer derartigen Elektrode
AU2001268468A1 (en) 2000-06-13 2001-12-24 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
JP3930227B2 (ja) * 2000-06-14 2007-06-13 ペンタックス株式会社 磁気式エンコーダおよび磁気式エンコーダを搭載した測量機
JP2002085097A (ja) 2000-09-12 2002-03-26 Hitachi Ltd Ｄｎａ塩基配列決定法
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
DE60131903T2 (de) 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
US20020055102A1 (en) * 2000-10-24 2002-05-09 David Stern Apparatus and method for scanning multiple arrays of biological probes
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
US20020074920A1 (en) 2000-12-15 2002-06-20 Chiu Randolph Kwok-Kin High efficiency, extended life spark plug having improved firing tips
US6783943B2 (en) 2000-12-20 2004-08-31 The Regents Of The University Of California Rolling circle amplification detection of RNA and DNA
US20020094536A1 (en) * 2000-12-28 2002-07-18 Cell Therapeutics, Inc. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis
EP1356119B1 (en) 2001-01-30 2007-09-26 Solexa Ltd. The preparation of polynucleotide arrays
WO2002061143A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Comparative analysis of nucleic acids using population tagging
EP2801624B1 (en) 2001-03-16 2019-03-06 Singular Bio, Inc Arrays and methods of use
JP4171206B2 (ja) 2001-03-16 2008-10-22 株式会社デンソー スパークプラグおよびその製造方法
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
GB0114853D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happier Mapping
EP2218762A3 (en) 2001-07-20 2010-09-29 Life Technologies Corporation Luminescent nanoparticles and methods for their preparation
EP1417474B1 (en) 2001-07-25 2021-12-29 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20050019766A1 (en) 2001-07-27 2005-01-27 Jinliu Feng Isp-1 and ctb-1 genes and uses thereof
US6881579B2 (en) 2001-07-30 2005-04-19 Agilent Technologies, Inc. Sample processing apparatus and methods
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
WO2005016869A1 (en) * 2003-08-19 2005-02-24 Postech Foundation Novel dendrimer compound, a biochip using the same and a fabricating method thereof
US9201067B2 (en) * 2003-03-05 2015-12-01 Posco Size-controlled macromolecule
US6975943B2 (en) 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
GB0126887D0 (en) 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
US7208295B2 (en) 2001-11-19 2007-04-24 Affymetrix, Inc. Multiplex oligonucleotide addition and target amplification
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
US7361310B1 (en) * 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
US7011945B2 (en) 2001-12-21 2006-03-14 Eastman Kodak Company Random array of micro-spheres for the analysis of nucleic acids
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
WO2004011665A2 (en) 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
AUPS298102A0 (en) 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
US7046376B2 (en) 2002-07-05 2006-05-16 Therma-Wave, Inc. Overlay targets with isolated, critical-dimension features and apparatus to measure overlay
TW200403052A (en) 2002-07-17 2004-03-01 Novartis Ag Use of organic compounds
US6747331B2 (en) * 2002-07-17 2004-06-08 International Business Machines Corporation Method and packaging structure for optimizing warpage of flip chip organic packages
WO2004017376A2 (en) * 2002-08-16 2004-02-26 Miragene, Inc. Integrated system for printing, processing, and imaging protein microarrays
AU2003259350A1 (en) † 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
WO2004025751A2 (en) 2002-09-11 2004-03-25 Moltech Invent S.A. Non-carbon anodes for aluminium electrowinning and other oxidation resistant components with iron oxide-containing coatings
AU2003267583A1 (en) 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
JP2006500959A (ja) 2002-09-30 2006-01-12 パラレル バイオサイエンス， インコーポレイテッド 動的サンプリング結合によるポリヌクレオチド合成および標識化
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
WO2004033728A2 (en) 2002-10-11 2004-04-22 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for pcr-based clonality studies
US20040120861A1 (en) * 2002-10-11 2004-06-24 Affymetrix, Inc. System and method for high-throughput processing of biological probe arrays
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
JP4395133B2 (ja) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
DE10300599A1 (de) 2003-01-10 2004-07-22 Jörg Dr. Sommer Mehrrumpfiges Schiff mit beweglichen Schwimmkörpern als Wellenkraftwerk
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
CA2515938A1 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
CN1791682B (zh) 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7718403B2 (en) 2003-03-07 2010-05-18 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
FR2852605B1 (fr) 2003-03-18 2012-11-30 Commissariat Energie Atomique Procede de preparation de fragments d'adn et ses applications
JP2004286579A (ja) 2003-03-20 2004-10-14 Kanagawa Acad Of Sci & Technol Ｄｎａ分析用アレイおよびそれを用いたｄｎａ分析システムおよび分析方法
EP1616947B1 (en) 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
KR100523767B1 (ko) 2003-06-12 2005-10-26 한국과학기술원 유기 초분자의 자기조립과 자외선 에칭을 이용한나노패턴의 형성방법
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259118A1 (en) 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
US7416911B2 (en) 2003-06-24 2008-08-26 California Institute Of Technology Electrochemical method for attaching molecular and biomolecular structures to semiconductor microstructures and nanostructures
AU2003903295A0 (en) 2003-06-30 2003-07-10 Raustech Pty Ltd Substrate for combinatorial chemistry
WO2005001120A1 (en) 2003-06-30 2005-01-06 Raustech Pty Ltd Micro and nano scale fabrication and manufacture by spatially selective deposition
US7326380B2 (en) 2003-07-18 2008-02-05 Northwestern University Surface and site-specific polymerization by direct-write lithography
GB0320337D0 (en) 2003-08-29 2003-10-01 Syrris Ltd A microfluidic system
US7824856B2 (en) 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
US7781378B2 (en) 2003-09-12 2010-08-24 Cornell Research Foundation, Inc. Protective coating for array material deposition
AT501110A1 (de) * 2003-09-16 2006-06-15 Upper Austrian Res Gmbh Arrays zur bindung von molekülen
US8222005B2 (en) 2003-09-17 2012-07-17 Agency For Science, Technology And Research Method for gene identification signature (GIS) analysis
AU2003273108A1 (en) * 2003-10-24 2005-05-11 Posco Novel dendrimer compound and a biochip using the same
EP1685380A2 (en) 2003-09-18 2006-08-02 Parallele Bioscience, Inc. System and methods for enhancing signal-to-noise ratios of microarray-based measurements
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US20050221341A1 (en) 2003-10-22 2005-10-06 Shimkets Richard A Sequence-based karyotyping
TWM245024U (en) * 2003-10-30 2004-10-01 Tranmax Machinery Co Ltd Airtight structure for air passage of pneumatic tool
US7148361B2 (en) 2003-10-31 2006-12-12 North Carolina State University Synthesis of phosphono-substituted porphyrin compounds for attachment to metal oxide surfaces
JP3796607B2 (ja) 2003-10-31 2006-07-12 株式会社リコー 定着装置
ATE510024T1 (de) 2003-10-31 2011-06-15 Advanced Genetic Analysis Corp Ab Verfahren zur herstellung eines gepaarten tag aus einer nukleinsäuresequenz sowie verfahren zur verwendung davon
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7854108B2 (en) 2003-12-12 2010-12-21 Vision Robotics Corporation Agricultural robot system and method
US7972994B2 (en) * 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
US20050136414A1 (en) 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
EP2789383B1 (en) * 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
GB0402895D0 (en) 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
CA2557841A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US20050214840A1 (en) 2004-03-23 2005-09-29 Xiangning Chen Restriction enzyme mediated method of multiplex genotyping
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US7470517B2 (en) 2004-04-09 2008-12-30 Trustees Of Boston University Method for de novo detection of sequences in nucleic acids: target sequencing by fragmentation
WO2005112565A2 (en) 2004-04-28 2005-12-01 University Of Houston Preparation of nanometric arrays of biomolecules on oligo- or poly(ethylene glycol) films on silicon surfaces
US20050260609A1 (en) 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
EP1766090B1 (en) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US7565346B2 (en) 2004-05-31 2009-07-21 International Business Machines Corporation System and method for sequence-based subspace pattern clustering
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
EP1647600A3 (en) 2004-09-17 2006-06-28 Affymetrix, Inc. (A US Entity) Methods for identifying biological samples by addition of nucleic acid bar-code tags
US20080050659A1 (en) 2004-09-30 2008-02-28 Japan Science And Technology Agency Method of Patterning Self-Organizing Material, Patterned Substrate of Self-Organizing Material and Method of Producing the Same, and Photomask Using Patterned Substrate of Self-Organizing Material
GB0422551D0 (en) 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
US20060110764A1 (en) 2004-10-25 2006-05-25 Tom Tang Large-scale parallelized DNA sequencing
JP2008520975A (ja) 2004-11-16 2008-06-19 ヘリコス バイオサイエンシーズ コーポレイション Ｔｉｒｆ単分子分析および核酸を配列決定する方法
WO2006055621A2 (en) 2004-11-17 2006-05-26 The University Of Chicago Histone deacetylase inhibitors and methods of use
WO2006061600A1 (en) 2004-12-07 2006-06-15 University Of Southampton Particles for use as a solid support and process for their preparation
WO2006074351A2 (en) 2005-01-05 2006-07-13 Agencourt Personal Genomics Reversible nucleotide terminators and uses thereof
EP2857523A1 (en) 2005-02-01 2015-04-08 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8569042B2 (en) 2005-02-23 2013-10-29 Alcatel Lucent DNA structures on ferroelectrics and semiconductors
US20060223122A1 (en) 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
US8110196B2 (en) 2005-04-29 2012-02-07 Polytopas LLC Methods and compositions for polytopic vaccination
US20060263789A1 (en) * 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
DK1907571T3 (da) 2005-06-15 2017-08-21 Complete Genomics Inc Nukleinsyreanalyse ved hjælp af tilfældige blandinger af ikke-overlappende fragmenter
US20070099212A1 (en) 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing
JP2009525492A (ja) * 2005-08-01 2009-07-09 一秋 上川 英語音、および他のヨーロッパ言語音の表現方法と発音テクニックのシステム
EP1910688A4 (en) 2005-08-04 2010-03-03 Helicos Biosciences Corp MULTICHANNEL FLOW CELLS
DK1915446T3 (da) 2005-08-11 2017-09-11 Synthetic Genomics Inc In vitro rekombination fremgangsmåde
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
AU2012216376B2 (en) 2005-10-07 2015-08-13 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
AU2013202990B2 (en) 2005-10-07 2015-08-20 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP1951900B1 (en) 2005-10-07 2016-06-15 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US9012193B2 (en) 2005-10-10 2015-04-21 The Regents Of The University Of California Micro-bubble plate for patterning biological and non-biological materials
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP1987159B2 (en) 2006-02-08 2020-08-12 Illumina Cambridge Limited Method for sequencing a polynucleotide template
US8492098B2 (en) * 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
JP2009529876A (ja) * 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段
DE102006015433A1 (de) 2006-03-31 2007-10-04 Reuver, Hermannus S.F. Verfahren zur Aufbereitung von Rotschlamm in Böden sowie Anlage zur Durchführung des Verfahrens
US7359610B2 (en) 2006-04-03 2008-04-15 Adc Telecommunications, Inc. Cable manager including nestable radius limiter
US7569979B2 (en) 2006-04-07 2009-08-04 Federal-Mogul World Wide, Inc. Spark plug having spark portion provided with a base material and a protective material
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US7835871B2 (en) 2007-01-26 2010-11-16 Illumina, Inc. Nucleic acid sequencing system and method
CA2687171A1 (en) 2007-06-08 2008-12-11 Bio-Dixam, Llc Amplification method of methylated or unmethylated nucleic acid
US20100304378A1 (en) 2007-09-17 2010-12-02 Universite De Strasbourg Method for Detecting or Quantifying a Truncating Mutation
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
WO2009061840A1 (en) 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009089384A1 (en) 2008-01-09 2009-07-16 Life Technologies Method of making a paired tag library for nucleic acid sequencing
US20090298131A1 (en) 2008-02-19 2009-12-03 Intelligent Biosystems, Inc. Non-Emulsion Methods And Masked Biomolecules
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
JP5669213B2 (ja) 2008-07-18 2015-02-12 アイデアペイント インコーポレイテッドＩｄｅａｐａｉｎｔ，Ｉｎｃ． 書き込み消去可能表面用の周囲温度で硬化する溶剤系被覆物
US8407554B2 (en) 2009-02-03 2013-03-26 Complete Genomics, Inc. Method and apparatus for quantification of DNA sequencing quality and construction of a characterizable model system using Reed-Solomon codes
US9524369B2 (en) * 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CN102725424B (zh) 2010-01-25 2014-07-09 Rd生物科技公司 靶核酸的自折叠扩增
WO2011097424A1 (en) 2010-02-05 2011-08-11 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
EP2363502B1 (en) 2010-03-04 2017-02-15 miacom Diagnostics GmbH Enhanced multiplex FISH
CN202281746U (zh) 2010-03-06 2012-06-20 伊鲁米那股份有限公司 检测来自样品光信号的测定设备及其光学组件和光学系统
WO2012098599A1 (ja) 2011-01-17 2012-07-26 パナソニック株式会社 撮像装置
CN107368705B (zh) * 2011-04-14 2021-07-13 完整基因有限公司 分析生物体的基因组dna的方法和计算机系统
WO2012162161A1 (en) 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
US9006630B2 (en) 2012-01-13 2015-04-14 Altasens, Inc. Quality of optically black reference pixels in CMOS iSoCs
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11414702B2 (en) 2005-06-15 2022-08-16 Complete Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US12421544B2 (en) 2005-06-15 2025-09-23 Complete Genomics, Inc. Labeling strategy for use in DNA sequencing to facilitate assembly of sequence reads into longer fragments of a genome

Also Published As

Publication number Publication date
US20200392574A1 (en) 2020-12-17
US9637784B2 (en) 2017-05-02
WO2006138257A2 (en) 2006-12-28
US20140011688A1 (en) 2014-01-09
EP1907571B1 (en) 2017-04-26
IL188142A (en) 2015-10-29
DK2620510T4 (da) 2020-03-30
DK2620510T3 (da) 2017-01-30
JP5331476B2 (ja) 2013-10-30
US20130345071A1 (en) 2013-12-26
US20160017414A1 (en) 2016-01-21
WO2006138257A3 (en) 2008-12-11
US8133719B2 (en) 2012-03-13
US8765375B2 (en) 2014-07-01
US8445196B2 (en) 2013-05-21
CA2611743A1 (en) 2006-12-28
CA2611743C (en) 2019-12-31
US20170175184A1 (en) 2017-06-22
DK1907583T4 (da) 2020-01-27
EP1907583A4 (en) 2009-11-11
EP1907571A4 (en) 2009-10-21
CN101466847A (zh) 2009-06-24
US20200399695A1 (en) 2020-12-24
JP2016174608A (ja) 2016-10-06
JP5965108B2 (ja) 2016-08-03
US8771958B2 (en) 2014-07-08
US20070099208A1 (en) 2007-05-03
US20090311691A1 (en) 2009-12-17
AU2006259565B2 (en) 2011-01-06
US12421544B2 (en) 2025-09-23
US20110319281A1 (en) 2011-12-29
US9637785B2 (en) 2017-05-02
JP2011234723A (ja) 2011-11-24
US20090137414A1 (en) 2009-05-28
US11414702B2 (en) 2022-08-16
WO2006138284A2 (en) 2006-12-28
US20090137404A1 (en) 2009-05-28
CN101466847B (zh) 2014-02-19
JP2009500004A (ja) 2009-01-08
US20130345069A1 (en) 2013-12-26
US20130345068A1 (en) 2013-12-26
US12331354B2 (en) 2025-06-17
US10125392B2 (en) 2018-11-13
US8445194B2 (en) 2013-05-21
US20110071053A1 (en) 2011-03-24
EP2463386A2 (en) 2012-06-13
US20080234136A1 (en) 2008-09-25
AU2006259565A1 (en) 2006-12-28
US20220411866A1 (en) 2022-12-29
EP1907583A2 (en) 2008-04-09
US20220411865A1 (en) 2022-12-29
US8445197B2 (en) 2013-05-21
IL241986A0 (en) 2015-11-30
EP2620510A1 (en) 2013-07-31
US8673562B2 (en) 2014-03-18
EP2620510B1 (en) 2016-10-12
US20130310264A1 (en) 2013-11-21
US20180051333A1 (en) 2018-02-22
US9650673B2 (en) 2017-05-16
US7709197B2 (en) 2010-05-04
US20130345070A1 (en) 2013-12-26
US20170152554A1 (en) 2017-06-01
DK1907583T3 (da) 2017-01-16
EP2463386A3 (en) 2012-08-15
US20220162694A1 (en) 2022-05-26
EP1907571A2 (en) 2008-04-09
SG162795A1 (en) 2010-07-29
US20150159204A1 (en) 2015-06-11
US8771957B2 (en) 2014-07-08
HK1209795A1 (en) 2016-04-08
EP3492602A1 (en) 2019-06-05
EP1907583B1 (en) 2016-10-05
JP2014138597A (ja) 2014-07-31
US20140018246A1 (en) 2014-01-16
CA2611671C (en) 2013-10-08
US7901891B2 (en) 2011-03-08
US9944984B2 (en) 2018-04-17
US8765382B2 (en) 2014-07-01
US20240301486A1 (en) 2024-09-12
US20130316920A1 (en) 2013-11-28
CA2611671A1 (en) 2006-12-28
US20070072208A1 (en) 2007-03-29
EP2865766A1 (en) 2015-04-29
EP3257949A1 (en) 2017-12-20
US20200115748A1 (en) 2020-04-16
EP2463386B1 (en) 2017-04-12
US12331353B2 (en) 2025-06-17
WO2006138284A3 (en) 2007-12-13
US20240263230A1 (en) 2024-08-08
EP2620510B2 (en) 2020-02-19
DK2463386T3 (da) 2017-07-31
US10351909B2 (en) 2019-07-16
EP1907583B2 (en) 2019-10-23
IL188142A0 (en) 2008-03-20
US8765379B2 (en) 2014-07-01

Similar Documents

Publication Publication Date Title
US12421544B2 (en) Labeling strategy for use in DNA sequencing to facilitate assembly of sequence reads into longer fragments of a genome
HK1172060B (en) Nucleic acid analysis by random mixtures of non-overlapping fragments
HK1172060A (en) Nucleic acid analysis by random mixtures of non-overlapping fragments