DK2411511T3 - Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, som koder for dem Download PDF

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Publication number
DK2411511T3
DK2411511T3 DK10710035.6T DK10710035T DK2411511T3 DK 2411511 T3 DK2411511 T3 DK 2411511T3 DK 10710035 T DK10710035 T DK 10710035T DK 2411511 T3 DK2411511 T3 DK 2411511T3
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polypeptide
vol
preferred aspect
acid
sequence
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DK10710035.6T
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Johan Börjesson
Anders Viksoe-Nielsen
Peter Kamp Hansen
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01006—Acetylesterase (3.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Claims (2)

1. Anvendelse af et polypeptid med acetylxylanesteraseaktivitet, som er valgt fra gruppen bestående af (i) et polypeptid, der omfatter en aminosyresekvens, som udviser mindst 85% identitet med det modne polypeptid ifølge SEQ ID NO: 2; og (ii) et polypeptid, som kodes for af et polynukleotid, der omfatter en nukleotidsekvens, som udviser mindst 85% identitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1, ved en fremgangsmåde til fremstilling af et fermenteringsprodukt ud fra lignocelluloseholdigt materiale, hvilken fremgangsmåde omfatter følgende trin: (a) forbehandling af lignocelluloseholdigt materiale; (b) hydrolyse af materialet i nærværelse af et cellulolytisk enzym og acetylxylanesterasen; (c) fermentering ved anvendelse af en fermenterende organisme.
2. Anvendelse af en sammensætning, som omfatter polypeptidet ifølge krav 1 og et eller flere yderligere enzymer valgt blandt xylanase, arabinofuranosidase, glucanase, pectinase, protease, acetylxylanesterase, ferulasyreesterase, rhamnogalacturonase og xylanase, ved en fremgangsmåde til fremstilling af et fermenteringsprodukt ud fra lignocelluloseholdigt materiale, hvilken fremgangsmåde omfatter følgende trin: (a) forbehandling af lignocelluloseholdigt materiale; (b) hydrolyse af materialet i nærværelse af et cellulolytisk enzym og acetylxylanesterasen; (c) fermentering ved anvendelse af en fermenterende organisme.
DK10710035.6T 2009-03-24 2010-03-23 Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, som koder for dem DK2411511T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09155966 2009-03-24
PCT/EP2010/053770 WO2010108918A1 (en) 2009-03-24 2010-03-23 Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same

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DK2411511T3 true DK2411511T3 (da) 2018-11-26

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US (1) US8658409B2 (da)
EP (1) EP2411511B1 (da)
CN (1) CN102365360A (da)
BR (1) BRPI1013483B1 (da)
CA (1) CA2752007A1 (da)
DK (1) DK2411511T3 (da)
WO (1) WO2010108918A1 (da)

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EP2411511A1 (en) 2012-02-01
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EP2411511B1 (en) 2018-08-08
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