DK2471902T3 - Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer - Google Patents

Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer Download PDF

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DK2471902T3
DK2471902T3 DK10811319.2T DK10811319T DK2471902T3 DK 2471902 T3 DK2471902 T3 DK 2471902T3 DK 10811319 T DK10811319 T DK 10811319T DK 2471902 T3 DK2471902 T3 DK 2471902T3
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Denmark
Prior art keywords
tissue
cells
artificial
agarose
collagen
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DK10811319.2T
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English (en)
Inventor
Mingorance Miguel Alaminos
Ávila José Ignacio Muñoz
Andrades Miguel González
Bello Ingrid Johanna Garzón
Muñoz Antonio Campos
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Servicio Andaluz De Salud
Univ Granada
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Priority claimed from ES200930625A external-priority patent/ES2353990B1/es
Priority claimed from ES200930943A external-priority patent/ES2362139B1/es
Application filed by Servicio Andaluz De Salud, Univ Granada filed Critical Servicio Andaluz De Salud
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Publication of DK2471902T3 publication Critical patent/DK2471902T3/da

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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
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    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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Claims (19)

1. In v/fro-fremgangsmåde til fremstilling af et kunstigt væv omfattende: a) tilsætning af et præparat omfattende fibrinogen til en prøve af isolerede celler, b) tilsætning af et antifibrinolytisk middel til det produkt, der fremkommer i trin (a), c) tilsætning af mindst én koagulationsfaktor, en kilde til calcium, thrombin eller en hvilken som helst kombination af ovenstående til det produkt, der fremkommer i trin (b), d) tilsætning af et præparat af et polysaccharid til det produkt, der fremkommer i trin (c), e) dyrkning af isolerede celler i eller på det produkt, der fremkommer i trin (d), og f) induktion af nanostrukturering af det produkt, der fremkommer i trin (e), hvor nanostruktureringsinduktionen omfatter dehydrering og/eller mekanisk komprimering af det produkt, der fremkommer i trin (e), hvor polysaccharidet i trin (d) er agarose.
2. Fremgangsmåde ifølge krav 1, hvor cellerne i trin (a) er fibroblaster eller keratocyt-ter.
3. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 2, der yderligere omfatter et trin (trin b2) mellem trin (b) og (c), hvor et protein tilsættes.
4. Fremgangsmåde ifølge krav 3, hvor det protein, der tilsættes i trin (b2), erfibronek-tin.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, der omfatter et trin (d2) mellem trin (d) og (e), som omfatter tilsætning af et præparat omfattende et protein til det produkt, der fremkommer i trin (d).
6. Fremgangsmåde ifølge krav 5, hvor det protein, der tilsættes i trin (d2), er kollagen.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor cellerne i trin (e) omfatter navlestrengsstamceller.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor cellerne i trin (e) omfatter epitelceller.
9. Fremgangsmåde ifølge krav 1, hvor dehydrering af det produkt, der fremkommer i trin (e), omfatter en fremgangsmåde, der er valgt fra listen omfattende: dræning, fordampning, sugning, kapillærtryk, osmose eller elektroosmose.
10. Fremgangsmåde ifølge krav 9, hvor dehydrering af det produkt, der fremkommer i trin (e) ved hjælp af kapillærtryk, omfatter påføring af et absorberende materiale på det produkt, der fremkommer i trin (e).
11. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 10, hvor den mekaniske kompression i trin (f) omfatter en fremgangsmåde, der er valgt fra listen omfattende: anvendelse af en statisk belastning, anvendelse af hydraulik, anvendelse af en kamskive, anvendelse af én eller flere valser, anvendelse afen ballon, ekstrudering eller centrifugering.
12. Fremgangsmåde ifølge krav 11, hvor anvendelsen afen statisk belastning i trin (f) omfatter placering af en vægt på det produkt, der fremkommer i trin (e).
13. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 12, hvor der mellem trin (e) og trin (f) er et yderligere trin, hvor det produkt, der fremkommer i trin (e), udsættes for luft.
14. Kunstigt væv opnået ved fremgangsmåden ifølge et hvilket som helst af kravene 1 til 13.
15. Anvendelse af det kunstige væv ifølge krav 14 til vurdering af et farmakologisk og/eller kemisk produkt.
16. Kunstigt væv ifølge krav 14 til anvendelse i medicin.
17. Kunstigt væv ifølge krav 14 til anvendelse til delvist eller fuldstændigt at forøge, genoprette eller udskifte den funktionelle aktivitet af et sygt eller beskadiget væv eller organ.
18. Kunstigt væv til anvendelse ifølge krav 17, hvor det beskadigede væv eller organet er valgt fra listen omfattende: hud, blære, urinrør, hornhinde, slimhinde, bindehinde, bugvæg, bindehinde, trommehinde, svælg, strubehoved, tarm, bughinde, ligament, sene, knogle, hjernehinde eller vagina.
19. Farmaceutisk præparat omfattende det kunstige væv ifølge krav 14.
DK10811319.2T 2009-08-25 2010-08-25 Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer DK2471902T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ES200930625A ES2353990B1 (es) 2009-08-25 2009-08-25 Elaboracion de tejidos artificiales mediante ingenieria tisular utilizando biomateriales de fibrina y agarosa.
ES200930943A ES2362139B1 (es) 2009-11-02 2009-11-02 Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina, agarosa y colágeno.
PCT/ES2010/070569 WO2011023843A2 (es) 2009-08-25 2010-08-25 Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa

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DK2471902T3 true DK2471902T3 (da) 2018-08-06

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US (2) US20120269776A1 (da)
EP (1) EP2471902B9 (da)
JP (2) JP6010460B2 (da)
DK (1) DK2471902T3 (da)
ES (1) ES2677945T3 (da)
PT (1) PT2471902T (da)
TR (1) TR201809713T4 (da)
WO (1) WO2011023843A2 (da)

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