DK2617825T3 - Varianter af glycosidhydrolase - Google Patents

Varianter af glycosidhydrolase Download PDF

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Publication number
DK2617825T3
DK2617825T3 DK12194265.0T DK12194265T DK2617825T3 DK 2617825 T3 DK2617825 T3 DK 2617825T3 DK 12194265 T DK12194265 T DK 12194265T DK 2617825 T3 DK2617825 T3 DK 2617825T3
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seq
amino acids
preferred
positions
substitutions
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DK12194265.0T
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English (en)
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Sarah Teter
Joel Cherry
Connie Ward
Paul Harris
Aubrey Jones
Jung Yi
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01176—Cellulose 1,4-beta-cellobiosidase (reducing end)(3.2.1.176)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (15)

1. Isoleret polypeptidvariant med glycosidhydrolaseaktivitet, som har en grad af identitet med aminosyreme 1 til 513 ifølge SEQ ID NO: 2 på mindst 90%, endnu mere fortrinsvis mindst. 95%, og mest fortrinsvis mindst 95%, hvor polypeptidets aminosyresekvens afviger fra SEQ ID NO: 2 i mindst én position svarende til position 94 blandt aminosyreme 1 til 513 ifølge SEQ ID NO: 2, hvor forskellen i mindst én position er en substitution i position 94, og varianten har forbedrede egenskaber sammenlignet med stamglycosidhydrolasen, hvor de forbedrede egenskaber er valgt fra gmppen bestående af temperaturafhængig aktivitetsprofil, termostabilitet, pH-aktivitet, pH-stabilitet og substratspecificitet, produktspecificitet og kemikaliebestandighed.
2. Polypeptidvariant ifølge krav 1, hvor substitutionen i polypeptidets aminosyresekvens i forhold til SEQ ID NO: 2 i en position svarende til position 94 er Ser, Ala, Arg or Gin.
3. Polypeptidvariant ifølge krav 1, hvor substitutionen i polypeptidets aminosyresekvens i forhold til SEQ ID NO: 2 i en position svarende til position 94 er Gly94Ser, Gly94Ala, Gly94Arg or Gly94Gln.
4. Polypeptidvariant ifølge et hvilket som helst af kravene 1-3, hvor polypeptidvarianten omfatter substitutioner i positioner svarende til position 94 og 226 blandt aminosyreme 1 til 513 ifølge SEQ ID NO: 2.
5. Po 1 ypeptidvariant ifølge krav 4, hvor varianten omfatter substitutionerne Gly94Ser (eller Gly94Ala eller Gly94Arg eller Gly94Gln) + Thr226Ala.
6. Isoleret nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-5.
7. Nukleinsyrekonstruktion, som omfatter nukleotidsekvensen ifølge krav 6.
8. Ekspressionsvektor, som omfatter nukleotidsekvensen ifølge krav 7.
9. Værtscelle, som omfatter nukleotidsekvensen ifølge krav 6.
10. Fremgangsmåde til fremstilling af en polypeptidvariant med glycosidhydrolaseaktivitet, hvilken fremgangsmåde omfatter (a) dyrkning af værtscellen ifølge krav 9 under betingelser, som er egnede til fremstilling af varianten; og (b) indvinding af varianten fra dyrkningsmediet.
11. Detergentsammensætning, som omfatter et polypeptid ifølge et hvilket som helst af kravene 1-5 og et overfladeaktivt middel.
12. Fremgangsmåde til nedbrydning af cellulose- og hemicelluloseholdig biomasse, hvilken fremgangsmåde omfatter behandling af biomassen med en effektiv mængde af et polypeptid ifølge et hvilket som helst af kravene 1-5 og indvinding af den nedbrudte biomasse.
13. Fremgangsmåde ifølge krav 12, som yderligere omfatter behandling af biomassen med en effektiv mængde af endo-l,4-beta-glucanase og exo-l,4-beta-D-glucanase.
14. Fremgangsmåde til nedbrydning af cellulose- og hemicelluloseholdig biomasse, hvilken fremgangsmåde omfatter behandling af biomassen med en værtscelle ifølge krav 9 og indvinding af den nedbrudte biomasse.
15. Fremgangsmåde ifølge krav 14, som yderligere omfatter behandling af biomassen med en effektiv mængde af endo-l,4-beta-glucanase og exo-l,4-beta-D-glucanase.
DK12194265.0T 2003-08-25 2004-08-25 Varianter af glycosidhydrolase DK2617825T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49780903P 2003-08-25 2003-08-25
EP11167129.3A EP2377931B1 (en) 2003-08-25 2004-08-25 Variants of glycoside hydrolases

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DK2617825T3 true DK2617825T3 (da) 2015-06-01

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DK12194268.4T DK2617826T3 (da) 2003-08-25 2004-08-25 Varianter af glucosidhydrolase
DK11167129.3T DK2377931T3 (da) 2003-08-25 2004-08-25 Varianter af glycosidhydrolase
DK12194265.0T DK2617825T3 (da) 2003-08-25 2004-08-25 Varianter af glycosidhydrolase

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DK12194268.4T DK2617826T3 (da) 2003-08-25 2004-08-25 Varianter af glucosidhydrolase
DK11167129.3T DK2377931T3 (da) 2003-08-25 2004-08-25 Varianter af glycosidhydrolase

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US (6) US20050048619A1 (da)
EP (5) EP2987857A1 (da)
CN (2) CN103088001A (da)
DK (3) DK2617826T3 (da)
ES (3) ES2424346T3 (da)
WO (1) WO2005030926A2 (da)

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DK3739045T3 (da) 2015-02-24 2024-10-21 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
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CN107779443B (zh) * 2017-10-26 2020-10-13 南开大学 纤维二糖水解酶突变体及其应用
CN109136124A (zh) * 2018-08-17 2019-01-04 西南科技大学 一种超富集植物体快速减容减量处理复合菌剂及其制备方法
CN109355275B (zh) * 2018-11-21 2021-08-20 中山大学 高热稳定性β-葡萄糖苷酶突变体及其应用
CN109456954B (zh) * 2018-11-21 2021-08-20 中山大学 热稳定性提高的β-葡萄糖苷酶突变体及其应用
CN114250214B (zh) * 2020-09-21 2023-07-07 南京工业大学 持续性内切纤维素酶突变体及其应用
CN112266907B (zh) * 2020-10-28 2022-03-15 江南大学 齐整小核菌内源小核菌胶水解酶及应用

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