DK2803722T3 - Mikroorganismer fra Corynebacterium, som kan udnytte xylose, og fremgangsmåde til fremstilling af L-lysin under anvendelse af samme - Google Patents
Mikroorganismer fra Corynebacterium, som kan udnytte xylose, og fremgangsmåde til fremstilling af L-lysin under anvendelse af samme Download PDFInfo
- Publication number
- DK2803722T3 DK2803722T3 DK13736186.1T DK13736186T DK2803722T3 DK 2803722 T3 DK2803722 T3 DK 2803722T3 DK 13736186 T DK13736186 T DK 13736186T DK 2803722 T3 DK2803722 T3 DK 2803722T3
- Authority
- DK
- Denmark
- Prior art keywords
- xylose
- lysine
- ala
- leu
- microorganism
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01017—Xylulokinase (2.7.1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01005—Xylose isomerase (5.3.1.5)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Chemical & Material Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (5)
1. Modificeret Corynebacterium sp.-mikroorganisme, der er i stand til at producere L-lysin ved anvendelse af xylose, hvor mikroorganismen omfatter Erwinia carotovora-afledt xyloseisomerase (XylA) og xylulokinase (XylB), hvor XylA omfatter aminosyresekvensen SEQ ID NO: 1 eller en aminosyresekvens med 95% eller mere identitet med aminosyresekvensen ifølge SEQ ID NO: 1, og hvor XylB omfatter aminosyresekvensen SEQ ID NO: 2 eller en aminosyresekvens med 90% eller mere identitet med aminosyresekvensen ifølge SEQ ID NO: 2.
2. Mikroorganisme ifølge krav 1, hvor XylA kodes af en polynukleotidsekvens med SEQ ID NO: 3, og XylB kodes afen polynukleotidsekvens med SEQ ID NO: 4.
3. Mikroorganisme ifølge krav 1, hvor mikroorganismen er Corynebacterium glutami-cum.
4. Fremgangsmåde til fremstilling afen mikroorganisme ifølge krav 1, hvor indføringen af XylA- og XylB-aktiviteter udføres ved at indsætte et polynukleotid, der omfatter nu-kleotidsekvenser, der koder for XylA og XylB, i et kromosom, indføre et vektorsystem, der omfatter polynukleotidet, i mikroorganismen opstrøms for de nukleotidsekvenser, der koder for XylA og XylB, indføre XylA og XylB med en modificeret promotor eller indføre de modificerede nukleotidsekvenser, som koder for XylA og XylB.
5. Fremgangsmåde til fremstilling af L-lysin, som omfatter følgende trin: (i) dyrkning af den modificerede Corynebacterium sp.-mikroorganisme, der er i stand til at fremstille L-lysin ved anvendelse af xylose, ifølge et hvilket som helst af kravene 1 til 3 i et dyrkningsmedium, der indeholder xylose som carbonkilde, for at opnå en dyrkningsvæske; og (ii) udvinding af L-lysin fra dyrkningsvæsken.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20120003133 | 2012-01-10 | ||
| PCT/KR2013/000221 WO2013105802A2 (ko) | 2012-01-10 | 2013-01-10 | 자일로즈 이용능이 부여된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-라이신의 생산방법 |
Publications (1)
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| DK2803722T3 true DK2803722T3 (da) | 2018-01-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| DK13736186.1T DK2803722T3 (da) | 2012-01-10 | 2013-01-10 | Mikroorganismer fra Corynebacterium, som kan udnytte xylose, og fremgangsmåde til fremstilling af L-lysin under anvendelse af samme |
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| US (2) | US9200300B2 (da) |
| EP (1) | EP2803722B1 (da) |
| JP (1) | JP5945336B2 (da) |
| KR (2) | KR20130082124A (da) |
| CN (2) | CN104245921B (da) |
| BR (1) | BR112014017088B1 (da) |
| DK (1) | DK2803722T3 (da) |
| ES (1) | ES2654809T3 (da) |
| MY (1) | MY164858A (da) |
| PL (1) | PL2803722T3 (da) |
| RU (1) | RU2584593C2 (da) |
| WO (1) | WO2013105802A2 (da) |
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| JP6519476B2 (ja) | 2013-10-23 | 2019-05-29 | 味の素株式会社 | 目的物質の製造法 |
| KR101755767B1 (ko) * | 2014-09-05 | 2017-07-10 | 씨제이제일제당 (주) | L-라이신 생산능이 향상된 미생물 및 이를 이용한 l-라이신 생산방법 |
| KR101694850B1 (ko) | 2014-10-08 | 2017-01-10 | 씨제이제일제당 주식회사 | L-글루타민을 생산하는 미생물 및 이를 이용한 l-글루타민 생산방법 |
| KR101793328B1 (ko) * | 2015-07-03 | 2017-11-03 | 씨제이제일제당 (주) | Ｌ-라이신 생산능을 갖는 미생물 및 이를 이용한 ｌ-라이신 생산 방법 |
| CA2991707C (en) | 2015-07-13 | 2023-08-01 | MARA Renewables Corporation | Enhancing microbial metabolism of c5 organic carbon |
| CN105567567A (zh) * | 2016-02-03 | 2016-05-11 | 程雪娇 | 一种甘蔗渣培养基及其制备方法 |
| EP3533875B1 (en) | 2016-09-01 | 2023-02-15 | Ningxia Eppen Biotech Co. Ltd | Corynebacterium for producing l-lysine by fermentation |
| KR101915433B1 (ko) * | 2018-02-13 | 2018-11-05 | 씨제이제일제당 (주) | 시트레이트 신타아제 (Citrate synthase)의 활성이 약화된 변이형 폴리펩타이드 및 이를 이용한 L-아미노산 생산방법 |
| CN110551648B (zh) * | 2018-05-30 | 2022-03-15 | 天津大学 | 发酵木糖生产琥珀酸的谷氨酸棒杆菌及用途 |
| KR102112240B1 (ko) | 2018-09-28 | 2020-05-18 | 씨제이제일제당 주식회사 | 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| AU2020414201B2 (en) * | 2019-12-23 | 2023-10-05 | Cj Cheiljedang Corporation | Microorganism for producing L-amino acid having increased cytochrome C activity, and L-amino acid production method using same |
| CN112877269B (zh) | 2020-01-15 | 2021-12-24 | 中国科学院天津工业生物技术研究所 | 生产赖氨酸的微生物以及赖氨酸的生产方法 |
| CN112458108A (zh) * | 2020-11-24 | 2021-03-09 | 华东理工大学 | 一种在谷氨酸棒状杆菌中利用木糖生成谷氨酸的合成路径的构建方法 |
| KR102277034B1 (ko) * | 2021-01-29 | 2021-07-13 | 씨제이제일제당 (주) | 신규한 dahp 신타아제 변이체 및 이를 이용한 l-라이신 생산 방법 |
| KR20220156323A (ko) * | 2021-05-18 | 2022-11-25 | 씨제이제일제당 (주) | Agl 단백질의 활성이 강화된, 아미노산을 생산하는 미생물 및 이를 이용한 아미노산 생산 방법 |
| CN115896152B (zh) * | 2021-09-30 | 2026-01-09 | 上海凯赛生物技术股份有限公司 | 一种谷氨酸棒状杆菌及其应用 |
| WO2024206168A1 (en) * | 2023-03-24 | 2024-10-03 | Natáur Llc | Bioproduction of sulfur-containing compounds |
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| KR100397322B1 (ko) | 2000-12-30 | 2003-09-06 | 씨제이 주식회사 | 엘-라이신의 제조방법 |
| SE0302421D0 (sv) * | 2003-09-11 | 2003-09-11 | Forskarpatent I Syd Ab | Construction of new xylose utilizing Saccharomyces cerevisiae strain |
| RU2283346C1 (ru) * | 2005-03-14 | 2006-09-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Способ получения l-аминокислот методом ферментации с использованием бактерий, обладающих повышенной экспрессией генов утилизации ксилозы |
| US8003367B2 (en) | 2004-03-16 | 2011-08-23 | Ajinomoto Co., Inc. | Method for producing L-amino acids by fermentation using bacteria having enhanced expression of xylose utilization genes |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
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| WO2008033001A1 (en) | 2006-09-15 | 2008-03-20 | Cj Cheiljedang Corporation | A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same |
| KR100838035B1 (ko) * | 2006-12-29 | 2008-06-12 | 씨제이제일제당 (주) | Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법 |
| JP2011515107A (ja) * | 2008-03-27 | 2011-05-19 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 改善されたキシロース資化能を有するザイモモナス（Ｚｙｍｏｍｏｎａｓ） |
| KR101126041B1 (ko) | 2008-04-10 | 2012-03-19 | 씨제이제일제당 (주) | 트랜스포존을 이용한 형질전환용 벡터, 상기 벡터로형질전환된 미생물 및 이를 이용한 ｌ-라이신 생산방법 |
| JP5564423B2 (ja) * | 2008-06-17 | 2014-07-30 | 公益財団法人地球環境産業技術研究機構 | Ｄ−キシロース利用機能が向上したコリネ型細菌形質転換体 |
| KR101083136B1 (ko) * | 2009-02-13 | 2011-11-11 | 씨제이제일제당 (주) | Ｌ-아미노산 생산용 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법 |
-
2013
- 2013-01-10 CN CN201380013346.9A patent/CN104245921B/zh active Active
- 2013-01-10 CN CN201810478368.7A patent/CN108467848A/zh active Pending
- 2013-01-10 BR BR112014017088-6A patent/BR112014017088B1/pt active IP Right Grant
- 2013-01-10 EP EP13736186.1A patent/EP2803722B1/en active Active
- 2013-01-10 JP JP2014552129A patent/JP5945336B2/ja active Active
- 2013-01-10 MY MYPI2014002035A patent/MY164858A/en unknown
- 2013-01-10 PL PL13736186T patent/PL2803722T3/pl unknown
- 2013-01-10 US US14/371,653 patent/US9200300B2/en active Active
- 2013-01-10 RU RU2014130234/10A patent/RU2584593C2/ru active
- 2013-01-10 DK DK13736186.1T patent/DK2803722T3/da active
- 2013-01-10 ES ES13736186.1T patent/ES2654809T3/es active Active
- 2013-01-10 WO PCT/KR2013/000221 patent/WO2013105802A2/ko not_active Ceased
- 2013-01-10 KR KR1020130003138A patent/KR20130082124A/ko not_active Withdrawn
-
2015
- 2015-04-13 KR KR1020150051878A patent/KR101592140B1/ko active Active
- 2015-10-27 US US14/923,825 patent/US9399784B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20150046778A (ko) | 2015-04-30 |
| EP2803722A2 (en) | 2014-11-19 |
| KR20130082124A (ko) | 2013-07-18 |
| JP5945336B2 (ja) | 2016-07-05 |
| CN108467848A (zh) | 2018-08-31 |
| CN104245921B (zh) | 2018-09-14 |
| RU2014130234A (ru) | 2016-02-27 |
| US9399784B2 (en) | 2016-07-26 |
| JP2015503359A (ja) | 2015-02-02 |
| PL2803722T3 (pl) | 2018-03-30 |
| EP2803722A4 (en) | 2015-07-22 |
| KR101592140B1 (ko) | 2016-02-22 |
| EP2803722B1 (en) | 2017-09-27 |
| ES2654809T3 (es) | 2018-02-15 |
| CN104245921A (zh) | 2014-12-24 |
| RU2584593C2 (ru) | 2016-05-20 |
| US9200300B2 (en) | 2015-12-01 |
| US20140377816A1 (en) | 2014-12-25 |
| US20160040200A1 (en) | 2016-02-11 |
| WO2013105802A2 (ko) | 2013-07-18 |
| BR112014017088A2 (pt) | 2017-06-13 |
| BR112014017088B1 (pt) | 2022-04-19 |
| MY164858A (en) | 2018-01-30 |
| WO2013105802A3 (ko) | 2013-09-19 |
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