DK2893012T3 - Polypeptider med proteaseaktivitet - Google Patents

Polypeptider med proteaseaktivitet Download PDF

Info

Publication number
DK2893012T3
DK2893012T3 DK13758862.0T DK13758862T DK2893012T3 DK 2893012 T3 DK2893012 T3 DK 2893012T3 DK 13758862 T DK13758862 T DK 13758862T DK 2893012 T3 DK2893012 T3 DK 2893012T3
Authority
DK
Denmark
Prior art keywords
ala
ser
gly
leu
thr
Prior art date
Application number
DK13758862.0T
Other languages
English (en)
Inventor
Mary Ann Stringer
Tine Hoff
Peter Rahbek Oestergaard
Fruergaard Katrine Pontoppidan
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2893012T3 publication Critical patent/DK2893012T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/22—Compounds of alkali metals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/20—Feeding-stuffs specially adapted for particular animals for horses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Insects & Arthropods (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (20)

1. Isoleret polypeptid med proteaseaktivitet, som er valgt fra gruppen bestående af: (a) et polypeptid, som udviser mindst 84% sekvensidentitet med polypeptidet ifølge SEQ ID NO: 5; (b) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 84% sekvensidentitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1; (c) en variant af polypeptidet ifølge SEQ ID NO: 5, som omfatter en substitution, deletion og/eller insertion i en eller flere positioner, hvor det samlede antal aminosyresubstitutioner, -deletioner og/eller -insertioner ikke er over 20; og (d) et fragment af et polypeptid ifølge (a), (b) eller (c), som har proteaseaktivitet, hvor polypeptidet har forbedret proteaseaktivitet mellem pH 3 og 4 ved 25°C i sammenligning med proteasen med sekvensen ifølge SEQ ID NO: 8 for stammen NRRL 18262.
2. Polypeptid ifølge krav 1, som omfatter eller består af SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5 eller SEQ ID NO: 6.
3. Sammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-2.
4. Sammensætning ifølge krav 3, som foreligger i form af et granulat eller et mikrogranulat.
5. Isoleret polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-2.
6. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter polynukleotidet ifølge krav 5, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsværtscelle.
7. Rekombinant ekspressionsværtscelle, som omfatter polynukleotidet ifølge krav 5, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet, hvor værten er valgt fra gruppen bestående af Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes og Trichoderma, eller hvor værten er en gær, såsom Pichia eller Saccharomyces, eller en Bacillus, såsom Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Geobacillus stearothermophilus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis eller Bacillus thuringiensis.
8. Værtscelle ifølge krav 7, hvor værten er en Aspergillus, såsom Aspergillus awamori, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger eller Aspergillus oryzae.
9. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1 -2, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform fremstiller polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
10. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-2, hvilken fremgangsmåde omfatter: (a) dyrkning af en værtscelle ifølge et hvilket som helst af kravene 7-8 under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
11. Dyrefodersammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1 -2.
12. Dyrefodersammensætning ifølge krav 11, som endvidere omfatter en eller flere amylaser, phytaser, xylanaser, galactanaser, alfa-galactosidaser, proteaser, phospholipaser, beta-glucanaser eller en hvilken som helst blanding deraf.
13. Dyrefodersammensætning ifølge krav 12, som endvidere omfatter mindst ét probiotikum eller en mikrobe til direkte tilførsel (DFM - direct fed microbial).
14. Anvendelse af mindst ét polypeptid ifølge et hvilket som helst af kravene 1-2 i dyrefoder; i dyrefoderadditiver; ved fremstilling af en sammensætning til anvendelse i dyrefoder; til forbedring af et dyrefoders næringsværdi; til forøgelse af fordøjeligt og/eller opløseligt protein i dyrefoder; til forøgelse af hydrolysegraden for proteiner i dyrefoder og/eller til behandling af proteiner.
15. Fremgangsmåde til forbedring af et dyrefoders næringsværdi, hvor mindst ét polypeptid ifølge et hvilket som helst af kravene 1-2 tilsættes til foderet.
16. Dyrefoderadditiv, som omfatter mindst ét polypeptid ifølge et hvilket som helst af kravene 1-2; og mindst ét fedtopløseligt vitamin; og/eller mindst ét vandopløseligt vitamin og/eller mindst ét spormineral.
17. Dyrefoderadditiv ifølge krav 16, som endvidere omfatter en eller flere amylaser, phytaser, xylanaser, galactanaser, alfa-galactosidaser, proteaser, phospholipaser, beta-glucanaser eller en hvilken som helst blanding deraf.
18. Dyrefoder, som har et råproteinindhold på 50 til 800 g/kg og omfatter mindst ét polypeptid ifølge et hvilket som helst af kravene 1-2.
19. Fremgangsmåde til behandling af proteiner, hvilken fremgangsmåde omfatter trinet til tilsætning af mindst ét polypeptid ifølge et hvilket som helst af kravene 1-2 til mindst ét/én protein eller proteinkilde.
20. Fremgangsmåde ifølge krav 19, hvor sojabønne eller sojabønnemel er indbefattet blandt den mindst ene proteinkilde.
DK13758862.0T 2012-09-05 2013-09-05 Polypeptider med proteaseaktivitet DK2893012T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12183079 2012-09-05
PCT/EP2013/068361 WO2014037438A1 (en) 2012-09-05 2013-09-05 Polypeptides having protease activity

Publications (1)

Publication Number Publication Date
DK2893012T3 true DK2893012T3 (da) 2018-12-03

Family

ID=49117854

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13758862.0T DK2893012T3 (da) 2012-09-05 2013-09-05 Polypeptider med proteaseaktivitet

Country Status (9)

Country Link
US (3) US9771570B2 (da)
EP (1) EP2893012B1 (da)
CN (2) CN104884615B (da)
AU (1) AU2013311668B2 (da)
BR (1) BR112015004701B1 (da)
DK (1) DK2893012T3 (da)
ES (1) ES2694765T3 (da)
MX (1) MX369726B (da)
WO (1) WO2014037438A1 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112016003339B1 (pt) 2013-08-30 2023-02-14 Novozymes A/S Composição de enzima, e, processo de produção de um produto de fermentação a partir de material contendo amido
CN105899543A (zh) * 2013-11-26 2016-08-24 诺维信公司 研磨方法
ES2810198T3 (es) 2014-06-27 2021-03-08 Dsm Ip Assets Bv Método para mejorar el valor nutricional del pienso para animales
US10676727B2 (en) 2015-06-18 2020-06-09 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
CN108350444A (zh) * 2015-09-25 2018-07-31 诺维信公司 丝氨酸蛋白酶用于改进乙醇产量的用途
JP7018402B2 (ja) * 2016-04-27 2022-02-10 バイオリソース インターナショナル，インコーポレイテッド 熱安定プロテアーゼ、並びにその生成方法及び使用方法
CN106045516B (zh) * 2016-06-12 2019-07-12 湖州优研知识产权服务有限公司 一种环保木质素陶瓷的制备工艺
CA3028875A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
MX2019000743A (es) 2016-07-21 2019-05-20 Novozymes As Variantes de serinproteasa y polinucleotidos que las codifican.
WO2018165252A1 (en) * 2017-03-07 2018-09-13 Bioresource International, Inc. Feed additive formulation and methods of making and using the same
US20220297171A1 (en) * 2017-04-14 2022-09-22 Novozymes A/S Processes For Solubilizing Municipal Solid Waste With Enzyme Compositions Comprising Protease And Enzyme Compositions Thereof
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
ES2908190T3 (es) 2017-06-28 2022-04-28 Novozymes As Polipéptidos con actividad de trehalasa y polinucleótidos que los codifican
CA3070281A1 (en) 2017-08-08 2019-02-14 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
CN111148841A (zh) 2017-08-30 2020-05-12 诺维信公司 M35家族的内切蛋白酶和s53家族的外切蛋白酶在淀粉发酵中的组合使用
CA3089135A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
EP4004029A1 (en) 2019-07-26 2022-06-01 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
AU2023213894B2 (en) * 2022-01-26 2025-04-10 Digestiva, Inc. Blood glucose stabilizing methods and compositions
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5443074B1 (da) * 1971-04-14 1979-12-18
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL97645A (en) 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DK91192D0 (da) 1992-07-10 1992-07-10 Novo Nordisk As Protein
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JP3678310B2 (ja) 1994-04-22 2005-08-03 ノボザイムス アクティーゼルスカブ 植物性タンパク質の溶解度を改善する方法
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
AU713323B2 (en) * 1996-03-01 1999-11-25 Novozymes A/S An enzyme with galactanase activity
US5840509A (en) * 1996-07-22 1998-11-24 Eli Lilly And Company Protease and related nucleic acid compounds
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
DK1124949T3 (da) 1998-10-26 2006-11-06 Novozymes As Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller
JP2002533133A (ja) 1998-12-23 2002-10-08 ノボザイムス アクティーゼルスカブ アスペルギルス変異体細胞におけるポリペプチドの生産方法
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
PL203212B1 (pl) 2000-02-08 2009-09-30 Dsm Ip Assets Bv Zastosowanie trwałej w kwasie proteazy w karmie zwierzęcej oraz w wytwarzaniu kompozycji do stosowania w karmie zwierzęcej, dodatek do karmy zwierzęcej, kompozycja karmy zwierzęcej i sposób obróbki białek roślinnych
AU2002223504A1 (en) 2000-11-17 2002-05-27 Novozymes A/S Heterologous expression of taxanes
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
WO2002068623A2 (en) 2001-02-23 2002-09-06 Dsm Ip Assets B.V. Genes encoding proteolytic enzymes from aspargilli
DE60226724D1 (de) 2001-05-04 2008-07-03 Novozymes As Antimikrobielles polypeptid
JP4361796B2 (ja) 2001-11-20 2009-11-11 ノボザイムス アクティーゼルスカブ シュードプレクタニア・ニグレラからの抗菌ポリペプチド
AU2002349297A1 (en) 2001-12-03 2003-06-17 Kobenhavns Amt Statin-like compounds
JP4567456B2 (ja) 2002-08-09 2010-10-20 ノース・キャロライナ・ステイト・ユニヴァーシティ 肉用家禽類の成長を改善するための方法および組成物
ES2533469T3 (es) 2002-10-11 2015-04-10 Novozymes A/S Método de preparación de un producto tratado con calor
WO2004072279A2 (en) 2003-02-07 2004-08-26 Novozymes A/S Proteases
ES2291883T3 (es) 2003-02-07 2008-03-01 Novozymes A/S Proteasas.
WO2004077960A1 (en) 2003-03-07 2004-09-16 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Use of proteolytic enzymes to increase feed utilization in ruminant diets
WO2004111223A1 (en) 2003-06-19 2004-12-23 Novozymes A/S Proteases
AU2004247801B2 (en) 2003-06-19 2010-09-23 Novozymes A/S Proteases
EP2308966A1 (en) 2003-10-10 2011-04-13 Novozymes A/S Protease variants
DE602004022967D1 (de) 2003-10-30 2009-10-15 Novozymes As Kohlenhydratbindende module
EP2258839B1 (en) 2004-06-21 2015-06-03 Novozymes A/S Proteases
US20110097779A1 (en) 2008-06-23 2011-04-28 Chee-Leong Soong Processes for Producing Fermentation Products
US20110165305A1 (en) 2008-07-18 2011-07-07 Novozymes A/S Method for preparing a protein hydrolysate
JP5416964B2 (ja) 2008-12-26 2014-02-12 ヤマキ株式会社 新規トリペプチドおよびそれらトリペプチドの製造法、ならびにアンジオテンシン変換酵素阻害物質の製造方法
CN101669584B (zh) 2009-10-16 2012-04-18 罗才鹏 一种猪用饲料增效添加剂及其制备方法
CN101816378A (zh) 2010-05-11 2010-09-01 蓝德安 养殖饲料添加剂
WO2012048334A2 (en) 2010-10-08 2012-04-12 Dyadic International (Usa) Inc. Novel fungal proteases

Also Published As

Publication number Publication date
US20180273927A1 (en) 2018-09-27
EP2893012B1 (en) 2018-08-15
MX2015002766A (es) 2015-05-15
BR112015004701B1 (pt) 2022-06-14
CN104884615A (zh) 2015-09-02
US20170342394A1 (en) 2017-11-30
AU2013311668B2 (en) 2019-02-28
US10006017B2 (en) 2018-06-26
AU2013311668A1 (en) 2015-02-26
BR112015004701A2 (pt) 2017-11-21
CN110229802A (zh) 2019-09-13
US9771570B2 (en) 2017-09-26
CN104884615B (zh) 2019-07-12
WO2014037438A1 (en) 2014-03-13
US10563188B2 (en) 2020-02-18
EP2893012A1 (en) 2015-07-15
MX369726B (es) 2019-11-20
US20150259662A1 (en) 2015-09-17
ES2694765T3 (es) 2018-12-27

Similar Documents

Publication Publication Date Title
US10563188B2 (en) Polypeptides having protease activity
AU2015341922B2 (en) Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed
US9441215B2 (en) Polypeptides having protease activity
US10174301B2 (en) Methods for improving the nutritional value of the animal feed using a protease
US9580703B2 (en) Polypeptides having protease activity