DK2970379T3 - Fremgangsmåder til forhøjelse af proteinrenhed ved anvendelse af protein a-baseret kromatografi - Google Patents
Fremgangsmåder til forhøjelse af proteinrenhed ved anvendelse af protein a-baseret kromatografi Download PDFInfo
- Publication number
- DK2970379T3 DK2970379T3 DK14722399.4T DK14722399T DK2970379T3 DK 2970379 T3 DK2970379 T3 DK 2970379T3 DK 14722399 T DK14722399 T DK 14722399T DK 2970379 T3 DK2970379 T3 DK 2970379T3
- Authority
- DK
- Denmark
- Prior art keywords
- protein
- procedures
- based chromatography
- increasing
- increasing protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
- B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient or chromatofocusing, i.e. separation according to the isoelectric point pI
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361783381P | 2013-03-14 | 2013-03-14 | |
| PCT/US2014/021802 WO2014159064A1 (en) | 2013-03-14 | 2014-03-07 | Methods of increasing protein purity using protein a based chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2970379T3 true DK2970379T3 (da) | 2019-07-22 |
Family
ID=50680106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14722399.4T DK2970379T3 (da) | 2013-03-14 | 2014-03-07 | Fremgangsmåder til forhøjelse af proteinrenhed ved anvendelse af protein a-baseret kromatografi |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10472389B2 (da) |
| EP (1) | EP2970379B1 (da) |
| JP (1) | JP6285532B2 (da) |
| KR (1) | KR101838039B1 (da) |
| CN (1) | CN105073769B (da) |
| CA (1) | CA2903496C (da) |
| DK (1) | DK2970379T3 (da) |
| ES (1) | ES2735354T3 (da) |
| SG (1) | SG11201505901YA (da) |
| WO (1) | WO2014159064A1 (da) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2970379B1 (en) | 2013-03-14 | 2019-05-01 | EMD Millipore Corporation | Methods of increasing protein purity using protein a based chromatography |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| JPWO2017014261A1 (ja) * | 2015-07-22 | 2018-04-26 | 株式会社カネカ | 抗体様タンパク質の精製方法 |
| CN107849095A (zh) * | 2015-07-22 | 2018-03-27 | 株式会社钟化 | 酸性pH范围内的抗体结合能力降低的抗体结合性蛋白质 |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| CN109071613A (zh) | 2016-05-11 | 2018-12-21 | 通用电气医疗集团生物工艺研发股份公司 | 分离基质 |
| JP6987424B2 (ja) | 2016-05-11 | 2022-01-05 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを洗浄および／または消毒する方法 |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US11708390B2 (en) | 2016-05-11 | 2023-07-25 | Cytiva Bioprocess R&D Ab | Method of storing a separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| EP4038083A1 (en) * | 2019-10-04 | 2022-08-10 | Merck Patent GmbH | Purification of proteins and viral inactivation |
| JP2022550837A (ja) * | 2019-10-04 | 2022-12-05 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | プロテインａアフィニティークロマトグラフィーにおけるモノクローナル抗体の溶離 |
| JP7600656B2 (ja) * | 2019-12-16 | 2024-12-17 | 東ソー株式会社 | 抗体分離のための溶液 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT1308456E (pt) | 1998-05-06 | 2007-12-03 | Genentech Inc | Purificação de anticorpos por cromatografia de permuta iónica |
| EP1500661A1 (en) | 1998-06-01 | 2005-01-26 | Genetech, Inc. | Separation of protein monomers from aggregates by use of ion-exchange chromatography |
| WO2004087761A1 (ja) * | 2003-03-31 | 2004-10-14 | Kirin Beer Kabushiki Kaisha | ヒトモノクローナル抗体およびヒトポリクローナル抗体の精製 |
| EP2066419B1 (en) * | 2006-09-29 | 2015-11-11 | GE Healthcare Bio-Sciences AB | Chromatography ligand comprising domain c from staphyloccocus aureus protein a for antibody isolation |
| WO2008085988A1 (en) * | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| US8198409B2 (en) * | 2007-05-21 | 2012-06-12 | Nomadic Bioscience Co., Ltd. | Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin |
| US20100069617A1 (en) | 2008-09-12 | 2010-03-18 | Ge Healthcare Bio-Sciences Ab | Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions |
| SG195555A1 (en) * | 2008-12-24 | 2013-12-30 | Emd Millipore Corp | Caustic stable chromatography ligands |
| WO2010110288A1 (ja) * | 2009-03-24 | 2010-09-30 | 株式会社カネカ | 免疫グロブリンに親和性を有するタンパク質および免疫グロブリン結合性アフィニティーリガンド |
| JP5816170B2 (ja) * | 2009-06-26 | 2015-11-18 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 天然の免疫グロブリン形式を有する容易に単離される二重特異性抗体 |
| JP5787891B2 (ja) * | 2009-09-01 | 2015-09-30 | ジェネンテック， インコーポレイテッド | 改変されたプロテインａ溶離による向上したタンパク質精製 |
| EP3318571B1 (en) | 2011-03-16 | 2021-02-17 | F. Hoffmann-La Roche AG | Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase |
| SG186552A1 (en) * | 2011-06-08 | 2013-01-30 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
| US9657055B2 (en) * | 2011-11-30 | 2017-05-23 | Ge Healthcare Bioprocess R&D Ab | Affinity chromatography matrix |
| EP2970379B1 (en) | 2013-03-14 | 2019-05-01 | EMD Millipore Corporation | Methods of increasing protein purity using protein a based chromatography |
-
2014
- 2014-03-07 EP EP14722399.4A patent/EP2970379B1/en active Active
- 2014-03-07 SG SG11201505901YA patent/SG11201505901YA/en unknown
- 2014-03-07 DK DK14722399.4T patent/DK2970379T3/da active
- 2014-03-07 KR KR1020157022870A patent/KR101838039B1/ko active Active
- 2014-03-07 CA CA2903496A patent/CA2903496C/en active Active
- 2014-03-07 US US14/768,254 patent/US10472389B2/en active Active
- 2014-03-07 ES ES14722399T patent/ES2735354T3/es active Active
- 2014-03-07 WO PCT/US2014/021802 patent/WO2014159064A1/en not_active Ceased
- 2014-03-07 JP JP2016500853A patent/JP6285532B2/ja active Active
- 2014-03-07 CN CN201480013955.9A patent/CN105073769B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2970379A1 (en) | 2016-01-20 |
| CA2903496C (en) | 2020-01-14 |
| CA2903496A1 (en) | 2014-10-02 |
| CN105073769B (zh) | 2019-09-10 |
| WO2014159064A1 (en) | 2014-10-02 |
| JP2016518318A (ja) | 2016-06-23 |
| CN105073769A (zh) | 2015-11-18 |
| KR20150110731A (ko) | 2015-10-02 |
| KR101838039B1 (ko) | 2018-03-14 |
| EP2970379B1 (en) | 2019-05-01 |
| JP6285532B2 (ja) | 2018-02-28 |
| ES2735354T3 (es) | 2019-12-18 |
| US20160122385A1 (en) | 2016-05-05 |
| US10472389B2 (en) | 2019-11-12 |
| SG11201505901YA (en) | 2015-08-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HUS2400016I1 (hu) | FCRN elleni antitestek | |
| DK2970379T3 (da) | Fremgangsmåder til forhøjelse af proteinrenhed ved anvendelse af protein a-baseret kromatografi | |
| DK2972353T3 (da) | Forbedrede fremgangsmåder til udførelse af multipleksanalyser | |
| DK2996556T3 (da) | System til billedstyret procedure | |
| PT2970464T (pt) | Proteínas de ligação anti-lag-3 | |
| DK3083540T3 (da) | Fremgangsmåde til fremstilling af substituerede phenoxyphenylketoner | |
| DK3611262T3 (da) | Fremgangsmåder til sekvensering af immunrepertoire | |
| DK3545906T3 (da) | Indretning til udskiftning af hjerteklap | |
| DK3071046T3 (da) | Fremgangsmåde til ekstraktion af ærteproteiner | |
| DK3301115T3 (da) | Kontinuerlig flertrinsfremgangsmåde til oprensning af antistoffer | |
| DK2951203T3 (da) | Heterodimere proteiner | |
| IL243311A0 (en) | Novel antibody frameworks | |
| DK3054976T3 (da) | Anvendelse af semaphorin-4d-bindende molekyler til behandling af aterosklerose | |
| DK2992104T3 (da) | Fremgangsmåde til ekspression | |
| DK3014279T3 (da) | Fremgangsmåde til bestemmelse af proteinaggregater under anvendelse af overflade-fida | |
| DK3041576T3 (da) | Fremgangsmåder til at detektere nyresygdom | |
| HRP20190434T1 (hr) | Fuzijski protein | |
| DK3010895T3 (da) | Fremgangsmåde til fremstilling af 4-propargyleret aminobenzoxazinoner | |
| DK2969098T3 (da) | Fremgangsmåder til oprensning af antistoffer | |
| DK3050055T3 (da) | Gengivelse af flerkanalsaudio ved anvendelse af interpolerede matricer | |
| DK3434760T3 (da) | Fremgangsmåder til forøgelse af mannose-indholdet i rekombinante proteiner | |
| DK2970378T3 (da) | Hydrofob interaktionsproteinkromatografi under saltfrie betingelser | |
| DK3065790T3 (da) | Fremgangsmåder til fjernelse af alpha-galactose | |
| DK2983680T3 (da) | Forbedring af organer til transplantation | |
| DK2878528T3 (da) | Fremgangsmåde til motordreven landing |