DK3050961T5 - Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller - Google Patents

Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller Download PDF

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Publication number
DK3050961T5
DK3050961T5 DK14847286.3T DK14847286T DK3050961T5 DK 3050961 T5 DK3050961 T5 DK 3050961T5 DK 14847286 T DK14847286 T DK 14847286T DK 3050961 T5 DK3050961 T5 DK 3050961T5
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improving
efficiency
stem cells
pluripotent stem
inducing pluripotent
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DK14847286.3T
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DK3050961T3 (da
Inventor
Hiroshi Ban
Akihiro Iida
Mamoru Hasegawa
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Id Pharma Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/602—Sox-2
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/603—Oct-3/4
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/09—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from epidermal cells, from skin cells, from oral mucosa cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
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    • C12N2523/00—Culture process characterised by temperature
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18841—Use of virus, viral particle or viral elements as a vector
    • C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2830/00—Vector systems having a special element relevant for transcription
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Transplantation (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK14847286.3T 2013-09-24 2014-09-24 Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller DK3050961T5 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013197308 2013-09-24
PCT/JP2014/075248 WO2015046229A1 (ja) 2013-09-24 2014-09-24 多能性幹細胞の誘導効率を改善する方法

Publications (2)

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DK3050961T3 DK3050961T3 (da) 2023-08-14
DK3050961T5 true DK3050961T5 (da) 2024-10-14

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DK14847286.3T DK3050961T5 (da) 2013-09-24 2014-09-24 Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller

Country Status (7)

Country Link
US (1) US10975358B2 (da)
EP (1) EP3050961B1 (da)
JP (1) JP6543194B2 (da)
CN (1) CN105916980B (da)
AU (1) AU2014324233B2 (da)
DK (1) DK3050961T5 (da)
WO (1) WO2015046229A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6854461B2 (ja) * 2014-11-05 2021-04-07 国立大学法人九州大学 ウイルスベクター、ｉＰＳ細胞の作製方法およびコンストラクト
WO2017082174A1 (ja) * 2015-11-13 2017-05-18 株式会社Ｉｄファーマ 改良されたパラミクソウイルスベクター
US11136555B2 (en) 2017-03-01 2021-10-05 Elixirgen Scientific, Inc. Compositions and methods for differentiation of human pluripotent stem cells into desired cell types
WO2021138447A1 (en) * 2019-12-31 2021-07-08 Elixirgen Therapeutics, Inc. Temperature-based transient delivery of nucleic acids and proteins to cells and tissues
JP7805590B2 (ja) 2020-11-06 2026-01-26 株式会社Trans Chromosomics 可逆的不死化細胞の製造方法
JPWO2022138964A1 (da) * 2020-12-25 2022-06-30
JP2022104813A (ja) 2020-12-29 2022-07-11 アイ ピース，インコーポレイテッド リプログラミング因子を導入された細胞の培養方法
AU2023415203A1 (en) 2022-12-28 2025-08-07 National University Corporation Chiba University Method for regulating degree of cell differentiation

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US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
DK0864645T3 (da) 1995-10-31 2005-10-24 Dnavec Research Inc Negativstrenget RNA-virusvektor med autonom replikeringsaktivitet
DK0863202T3 (da) 1995-11-01 2010-09-27 Dnavec Research Inc Rekombinant Sendai-virus
KR100739938B1 (ko) 1999-05-18 2007-07-16 가부시키가이샤 디나벡크 겐큐쇼 엔벨로프 유전자 결손 파라믹소과 바이러스 벡터
ATE399206T1 (de) 1999-05-18 2008-07-15 Dnavec Research Inc Ribonukleoproteinkomplex aus paramyxovirus
EP1211318B1 (en) 1999-09-06 2007-08-08 DNAVEC Research, Inc. Paramyxoviruses comprising a modified transcription start sequence
JPWO2003025570A1 (ja) 2001-09-18 2004-12-24 株式会社ディナベック研究所 粒子形成能が低下した（−）鎖ｒｎａウィルスベクターの検査方法および製造方法
JP4999330B2 (ja) 2004-01-22 2012-08-15 株式会社ディナベック研究所 サイトメガロウイルスエンハンサーおよびニワトリβ−アクチンプロモーターを含むハイブリッドプロモーターを利用したマイナス鎖ＲＮＡウイルスベクターの製造方法
JP4901471B2 (ja) 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
WO2006137517A1 (ja) 2005-06-24 2006-12-28 Dnavec Corporation 幼少個体への遺伝子導入用ベクター
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
CA2636600A1 (en) 2006-01-17 2007-07-26 Dnavec Corporation Novel protein expression system
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Also Published As

Publication number Publication date
WO2015046229A1 (ja) 2015-04-02
EP3050961A1 (en) 2016-08-03
AU2014324233B2 (en) 2020-07-23
AU2014324233A1 (en) 2016-04-14
EP3050961A4 (en) 2017-03-08
CN105916980B (zh) 2025-09-16
DK3050961T3 (da) 2023-08-14
US20160215270A1 (en) 2016-07-28
CN105916980A (zh) 2016-08-31
EP3050961B1 (en) 2023-06-28
AU2014324233A2 (en) 2016-04-28
JPWO2015046229A1 (ja) 2017-03-09
JP6543194B2 (ja) 2019-07-10
US10975358B2 (en) 2021-04-13

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