DK3083676T3 - Hidtil ukendte eukaryote celler og fremgangsmåder til rekombinant ekspression af et produkt af interesse - Google Patents

Hidtil ukendte eukaryote celler og fremgangsmåder til rekombinant ekspression af et produkt af interesse Download PDF

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Publication number
DK3083676T3
DK3083676T3 DK14821859T DK14821859T DK3083676T3 DK 3083676 T3 DK3083676 T3 DK 3083676T3 DK 14821859 T DK14821859 T DK 14821859T DK 14821859 T DK14821859 T DK 14821859T DK 3083676 T3 DK3083676 T3 DK 3083676T3
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Denmark
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interest
methods
product
recombinant expression
eukaryotic cells
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DK14821859T
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English (en)
Inventor
Thomas Jostock
Holger Laux
Anett Ritter
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Novartis Ag
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Publication of DK3083676T3 publication Critical patent/DK3083676T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK14821859T 2013-12-20 2014-12-18 Hidtil ukendte eukaryote celler og fremgangsmåder til rekombinant ekspression af et produkt af interesse DK3083676T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361919313P 2013-12-20 2013-12-20
PCT/IB2014/067073 WO2015092735A1 (en) 2013-12-20 2014-12-18 Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Publications (1)

Publication Number Publication Date
DK3083676T3 true DK3083676T3 (da) 2019-11-25

Family

ID=52278687

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14821859T DK3083676T3 (da) 2013-12-20 2014-12-18 Hidtil ukendte eukaryote celler og fremgangsmåder til rekombinant ekspression af et produkt af interesse

Country Status (18)

Country Link
US (2) US11203631B2 (da)
EP (2) EP3083676B1 (da)
JP (1) JP6708548B2 (da)
KR (1) KR102288857B1 (da)
CN (1) CN106029692A (da)
AU (1) AU2014369175B2 (da)
BR (1) BR112016013443B1 (da)
CA (1) CA2934411C (da)
DK (1) DK3083676T3 (da)
ES (1) ES2758504T3 (da)
IL (1) IL245854B (da)
LT (1) LT3083676T (da)
MX (1) MX371025B (da)
PL (1) PL3083676T3 (da)
PT (1) PT3083676T (da)
RU (1) RU2720525C2 (da)
SG (1) SG11201604215XA (da)
WO (1) WO2015092735A1 (da)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2791758T3 (es) * 2011-12-22 2020-11-05 Hoffmann La Roche Organización de vectores de expresión, procedimientos de generación de células de producción novedosos y su uso para la producción recombinante de polipéptidos
JP6708548B2 (ja) 2013-12-20 2020-06-10 ノバルティス アーゲー 新規真核細胞および目的の生成物を組換え発現させるための方法
EP3083677B1 (en) 2013-12-20 2019-08-28 Novartis AG Novel eukaryotic cells and methods for recombinantly expressing a product of interest
EP3015475A1 (en) 2014-10-31 2016-05-04 Novartis AG Mammalian cells expressing cytomegalovirus antigens
EP3048114A1 (en) 2015-01-22 2016-07-27 Novartis AG Cytomegalovirus antigens and uses thereof
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
UY37758A (es) 2017-06-12 2019-01-31 Novartis Ag Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN107828738A (zh) * 2017-11-28 2018-03-23 新乡医学院 一种dna甲基转移酶缺陷型cho细胞系及其制备方法及应用
UY38299A (es) 2018-07-18 2020-02-28 Novartis Ag Apirasas solubilizadas, métodos y usos
JP7522100B2 (ja) * 2019-04-02 2024-07-24 中外製薬株式会社 標的特異的な外来遺伝子の導入方法
CN112852874B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac5基因敲除的bhk-21细胞系及其构建方法和应用

Family Cites Families (24)

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JP2585532B2 (ja) 1986-05-10 1997-02-26 三井東圧化学株式会社 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
IE913929A1 (en) 1990-11-13 1992-05-20 Immunex Corp Bifunctional selectable fusion genes
EP0724639B1 (en) 1991-03-29 2001-01-31 Genentech, Inc. Methods for selection of recombinant host cells expressing high levels of a desired protein
AU6953394A (en) 1993-05-21 1994-12-20 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
JP4031154B2 (ja) * 1999-07-30 2008-01-09 独立行政法人科学技術振興機構 遺伝子増幅細胞の迅速選択法
WO2003014361A1 (en) 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression
JP2005526517A (ja) 2002-05-22 2005-09-08 バイオジェン・アイデック・エムエイ・インコーポレイテッド 分泌性ポリペプチドの検出
WO2004035732A2 (en) 2002-08-29 2004-04-29 Five Prime Therapeutics, Inc. Human polypeptides encoded by polynucleotides and methods of their use
US7824907B2 (en) 2003-03-11 2010-11-02 Merck Serono Sa Expression vectors comprising the mCMV IE2 promoter
DK1716233T3 (da) 2004-01-30 2009-10-26 Maxygen Holdings Ltd Reguleret stopkodongennemlæsning
US20050272061A1 (en) 2004-02-19 2005-12-08 Seattle Genetics, Inc. Expression profiling in non-small cell lung cancer
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
EP2027273B1 (en) 2006-05-17 2016-04-13 F. Hoffmann-La Roche AG Polypeptide producing cells
AU2007352346B2 (en) * 2006-10-30 2012-12-06 Eli Lilly And Company Random homozygous gene perturbation to enhance antibody production
WO2008064095A1 (en) 2006-11-17 2008-05-29 3M Innovative Properties Company Ribbon curling device
EA018840B1 (ru) * 2007-02-15 2013-11-29 ДСМ АйПи АССЕТС Б.В. Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
KR101577839B1 (ko) * 2007-08-07 2015-12-15 추가이 세이야쿠 가부시키가이샤 이종 단백질의 제조 방법
BRPI0821401A2 (pt) 2007-12-21 2014-10-14 Novartis Ag Compostos orgânicos
RU2389628C1 (ru) * 2008-08-18 2010-05-20 Закрытое акционерное общество Научная организация "Тверской институт вагоностроения" (ЗАО НО "ТИВ") Привод тормоза вагона
AU2009286956B2 (en) 2008-08-28 2012-12-06 Novartis Ag Cell surface display of polypeptide isoforms by stop codon readthrough
JP5801207B2 (ja) 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
SG175779A1 (en) 2009-05-15 2011-12-29 Boehringer Ingelheim Int Improved cell lines having reduced expression of nocr and use thereof
EP3083677B1 (en) 2013-12-20 2019-08-28 Novartis AG Novel eukaryotic cells and methods for recombinantly expressing a product of interest
JP6708548B2 (ja) 2013-12-20 2020-06-10 ノバルティス アーゲー 新規真核細胞および目的の生成物を組換え発現させるための方法

Also Published As

Publication number Publication date
CA2934411A1 (en) 2015-06-25
EP3083676A1 (en) 2016-10-26
AU2014369175A1 (en) 2016-06-16
CA2934411C (en) 2022-05-03
RU2016129285A (ru) 2018-01-25
MX371025B (es) 2020-01-13
WO2015092735A1 (en) 2015-06-25
US20220298228A1 (en) 2022-09-22
EP3083676B1 (en) 2019-08-28
ES2758504T3 (es) 2020-05-05
AU2014369175B2 (en) 2017-03-02
KR20160101981A (ko) 2016-08-26
KR102288857B1 (ko) 2021-08-11
IL245854A0 (en) 2016-07-31
US11203631B2 (en) 2021-12-21
JP6708548B2 (ja) 2020-06-10
BR112016013443B1 (pt) 2022-11-29
JP2017500868A (ja) 2017-01-12
LT3083676T (lt) 2019-11-25
MX2016008249A (es) 2016-09-29
SG11201604215XA (en) 2016-07-28
PT3083676T (pt) 2019-11-20
CN106029692A (zh) 2016-10-12
RU2720525C2 (ru) 2020-04-30
BR112016013443A2 (pt) 2017-09-26
US20170002059A1 (en) 2017-01-05
PL3083676T3 (pl) 2020-02-28
IL245854B (en) 2021-06-30
EP3604331A1 (en) 2020-02-05

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