DK3180425T3 - Genetisk målretning i ikke-konventionel gær ved anvendelse af en rna- guidet endonuklease - Google Patents
Genetisk målretning i ikke-konventionel gær ved anvendelse af en rna- guidet endonuklease Download PDFInfo
- Publication number
- DK3180425T3 DK3180425T3 DK15747678.9T DK15747678T DK3180425T3 DK 3180425 T3 DK3180425 T3 DK 3180425T3 DK 15747678 T DK15747678 T DK 15747678T DK 3180425 T3 DK3180425 T3 DK 3180425T3
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- DK
- Denmark
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- rna
- conventional door
- guided endonuclease
- genetic targeting
- targeting
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/121—Hammerhead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462036652P | 2014-08-13 | 2014-08-13 | |
| PCT/US2015/041256 WO2016025131A1 (en) | 2014-08-13 | 2015-07-21 | Genetic targeting in non-conventional yeast using an rna-guided endonuclease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3180425T3 true DK3180425T3 (da) | 2020-06-29 |
Family
ID=53783981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15747678.9T DK3180425T3 (da) | 2014-08-13 | 2015-07-21 | Genetisk målretning i ikke-konventionel gær ved anvendelse af en rna- guidet endonuklease |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10513711B2 (da) |
| EP (2) | EP3180425B1 (da) |
| JP (1) | JP6811707B2 (da) |
| KR (1) | KR102381610B1 (da) |
| CN (1) | CN107002020B (da) |
| AU (1) | AU2015302213B2 (da) |
| CA (1) | CA2957684C (da) |
| DK (1) | DK3180425T3 (da) |
| WO (1) | WO2016025131A1 (da) |
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| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| EP3690044B1 (en) | 2014-02-11 | 2024-01-10 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016073559A1 (en) * | 2014-11-05 | 2016-05-12 | The Regents Of The University Of California | Methods for autocatalytic genome editing and neutralizing autocatalytic genome editing |
| JP6823593B2 (ja) | 2014-11-06 | 2021-02-03 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | Ｒｎａ誘導型エンドヌクレアーゼの細胞へのペプチド媒介輸送 |
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| US7459546B2 (en) | 2003-06-25 | 2008-12-02 | E.I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast |
| WO2005049805A2 (en) | 2003-11-14 | 2005-06-02 | E.I. Dupont De Nemours And Company | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast |
| US7264949B2 (en) | 2004-09-15 | 2007-09-04 | E.I. Du Pont De Nemours And Company | Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast |
| FR2877011B1 (fr) | 2004-10-27 | 2010-08-27 | Centre Nat Rech Scient | Souche de levure genetiquement modifiee presentant une production et une excretion accrue de s-adenosylmethionine (sam) |
| US20060094102A1 (en) | 2004-11-04 | 2006-05-04 | Zhixiong Xue | Ammonium transporter promoter for gene expression in oleaginous yeast |
| DK2341149T3 (da) | 2005-08-26 | 2017-02-27 | Dupont Nutrition Biosci Aps | Anvendelse af CRISPR-associerede gener (Cas) |
| US20090117253A1 (en) | 2007-10-03 | 2009-05-07 | E. I. Du Pont De Nemours And Company | Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms |
| WO2010025374A2 (en) | 2008-08-29 | 2010-03-04 | E. I. Du Pont De Nemours And Company | Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms |
| US8637298B2 (en) | 2009-06-16 | 2014-01-28 | E I Du Pont De Nemours And Company | Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
| US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
| EP2609203B1 (en) | 2010-08-26 | 2018-06-20 | E. I. du Pont de Nemours and Company | Recombinant microbial host cells for high eicosapentaenoic acid production |
| EP2691518A1 (en) | 2011-03-31 | 2014-02-05 | E.I. Du Pont De Nemours And Company | Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast |
| EP2694665A1 (en) | 2011-04-01 | 2014-02-12 | E. I. Du Pont de Nemours and Company | Yarrowia esterase/lipase promoter regions for gene expression in yeast |
| US8735094B2 (en) | 2011-04-05 | 2014-05-27 | E I Du Pont De Nemours And Company | Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast |
| EP2702148A1 (en) | 2011-04-07 | 2014-03-05 | E.I. Du Pont De Nemours And Company | Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast |
| BR112014006394A2 (pt) * | 2011-09-19 | 2017-03-28 | Kymab Ltd | manipulação de diversidade genética de imunoglobulina e terapêuticos multi-anticorpos |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| EP2840140B2 (en) | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| CA2893409A1 (en) | 2012-12-21 | 2014-06-26 | E. I. Du Pont De Nemours And Company | Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight |
| CN120574876A (zh) | 2013-08-22 | 2025-09-02 | 纳幕尔杜邦公司 | 使用向导rna/cas内切核酸酶系统的植物基因组修饰及其使用方法 |
| US20170088845A1 (en) | 2014-03-14 | 2017-03-30 | The Regents Of The University Of California | Vectors and methods for fungal genome engineering by crispr-cas9 |
-
2015
- 2015-07-05 US US15/503,383 patent/US10513711B2/en active Active
- 2015-07-21 CN CN201580056204.XA patent/CN107002020B/zh not_active Expired - Fee Related
- 2015-07-21 EP EP15747678.9A patent/EP3180425B1/en active Active
- 2015-07-21 JP JP2017507714A patent/JP6811707B2/ja not_active Expired - Fee Related
- 2015-07-21 EP EP20158059.4A patent/EP3686278A1/en not_active Withdrawn
- 2015-07-21 KR KR1020177006429A patent/KR102381610B1/ko active Active
- 2015-07-21 WO PCT/US2015/041256 patent/WO2016025131A1/en not_active Ceased
- 2015-07-21 DK DK15747678.9T patent/DK3180425T3/da active
- 2015-07-21 AU AU2015302213A patent/AU2015302213B2/en not_active Ceased
- 2015-07-21 CA CA2957684A patent/CA2957684C/en active Active
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2019
- 2019-10-30 US US16/668,528 patent/US20200190540A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| CN107002020A (zh) | 2017-08-01 |
| CN107002020B (zh) | 2021-12-21 |
| AU2015302213A9 (en) | 2019-08-01 |
| AU2015302213A1 (en) | 2017-03-02 |
| WO2016025131A1 (en) | 2016-02-18 |
| CA2957684C (en) | 2023-02-21 |
| US10513711B2 (en) | 2019-12-24 |
| EP3686278A1 (en) | 2020-07-29 |
| EP3180425A1 (en) | 2017-06-21 |
| US20170226533A1 (en) | 2017-08-10 |
| JP6811707B2 (ja) | 2021-01-13 |
| KR20170036792A (ko) | 2017-04-03 |
| AU2015302213B2 (en) | 2021-05-13 |
| EP3180425B1 (en) | 2020-03-25 |
| US20200190540A1 (en) | 2020-06-18 |
| KR102381610B1 (ko) | 2022-04-01 |
| JP2017522897A (ja) | 2017-08-17 |
| WO2016025131A8 (en) | 2017-06-08 |
| CA2957684A1 (en) | 2016-02-18 |
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