DK3224374T3 - Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering - Google Patents
Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering Download PDFInfo
- Publication number
- DK3224374T3 DK3224374T3 DK15797459.3T DK15797459T DK3224374T3 DK 3224374 T3 DK3224374 T3 DK 3224374T3 DK 15797459 T DK15797459 T DK 15797459T DK 3224374 T3 DK3224374 T3 DK 3224374T3
- Authority
- DK
- Denmark
- Prior art keywords
- fragmenting
- sequencing
- nucleic acid
- nucleic
- acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/331—Methylation site specific nuclease
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1421003.3A GB2532749B (en) | 2014-11-26 | 2014-11-26 | Method for preparing a nucleic acid for sequencing using MspJI family restriction endonucleases |
| PCT/IB2015/058762 WO2016083933A1 (en) | 2014-11-26 | 2015-11-12 | Method for fragmenting a nucleic acid for sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3224374T3 true DK3224374T3 (da) | 2020-12-21 |
Family
ID=52292558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15797459.3T DK3224374T3 (da) | 2014-11-26 | 2015-11-12 | Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9920355B2 (da) |
| EP (1) | EP3224374B1 (da) |
| DK (1) | DK3224374T3 (da) |
| ES (1) | ES2836599T3 (da) |
| GB (1) | GB2532749B (da) |
| WO (1) | WO2016083933A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2812261T3 (es) | 2016-08-01 | 2021-03-16 | Hoffmann La Roche | Procedimientos para la retirada de dímeros de adaptadores de preparaciones para la secuenciación de ácidos nucleicos |
| WO2018195224A1 (en) * | 2017-04-18 | 2018-10-25 | Fred Hutchinson Cancer Research Center | Barcoded transposases to increase efficiency of high-accuracy genetic sequencing |
| JP7056012B2 (ja) | 2017-05-19 | 2022-04-19 | トヨタ自動車株式会社 | ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法 |
| US11021701B2 (en) | 2017-11-22 | 2021-06-01 | New England Biolabs, Inc. | Method for fragmenting DNA by nick translation |
| JP7047373B2 (ja) | 2017-12-25 | 2022-04-05 | トヨタ自動車株式会社 | 次世代シーケンサー用プライマー並びにその製造方法、次世代シーケンサー用プライマーを用いたｄｎａライブラリー並びにその製造方法、及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法 |
| US11680290B2 (en) * | 2019-12-19 | 2023-06-20 | Chapter Diagnostics, Inc. | Efficient methods and compositions for multiplex target amplification PCR |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| CA3165865A1 (en) * | 2019-12-23 | 2021-07-01 | Biofidelity Ltd | Kits and devices |
| US11280028B1 (en) * | 2021-02-24 | 2022-03-22 | Agency For Science, Technology And Research (A*Star) | Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid |
| CN115873922B (zh) * | 2021-09-28 | 2025-04-29 | 深圳华大智造科技股份有限公司 | 一种单细胞全长转录本建库测序方法 |
| WO2023172520A2 (en) * | 2022-03-07 | 2023-09-14 | Twist Bioscience Corporation | Methylation-mediated adapter removal on nucleic acid sequences |
| CN114686455B (zh) * | 2022-05-16 | 2023-04-18 | 江苏百时美生物科技有限公司 | 一种混合限制酶及其应用 |
| CN119923475A (zh) * | 2022-07-21 | 2025-05-02 | 夸登特健康公司 | 用于检测和减少样品制备诱导的甲基化人为假象的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1606417A2 (en) | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna |
| US8592150B2 (en) * | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2010075375A1 (en) * | 2008-12-23 | 2010-07-01 | New England Biolabs, Inc. | Compositions, methods and related uses for cleaving modified dna |
| GB201003036D0 (en) * | 2010-02-23 | 2010-04-07 | Fermentas Uab | Restriction endonucleases and their applications |
| JP5917519B2 (ja) * | 2010-09-10 | 2016-05-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | クロマチン分析のためのｄｎａのサイズ選択 |
| US20140178873A1 (en) * | 2011-03-04 | 2014-06-26 | Andreas Brachmann | Novel methods for detecting hydroxymethylcytosine |
| CA2892043C (en) | 2012-11-21 | 2022-01-11 | Courtagen Life Sciences Inc. | A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample |
-
2014
- 2014-11-26 GB GB1421003.3A patent/GB2532749B/en active Active
-
2015
- 2015-11-12 ES ES15797459T patent/ES2836599T3/es active Active
- 2015-11-12 US US15/526,740 patent/US9920355B2/en active Active
- 2015-11-12 DK DK15797459.3T patent/DK3224374T3/da active
- 2015-11-12 WO PCT/IB2015/058762 patent/WO2016083933A1/en not_active Ceased
- 2015-11-12 EP EP15797459.3A patent/EP3224374B1/en active Active
-
2018
- 2018-02-09 US US15/893,507 patent/US10472667B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB2532749B (en) | 2016-12-28 |
| ES2836599T3 (es) | 2021-06-25 |
| WO2016083933A1 (en) | 2016-06-02 |
| US9920355B2 (en) | 2018-03-20 |
| US20170335371A1 (en) | 2017-11-23 |
| GB201421003D0 (en) | 2015-01-07 |
| US10472667B2 (en) | 2019-11-12 |
| GB2532749A (en) | 2016-06-01 |
| US20180305737A1 (en) | 2018-10-25 |
| EP3224374B1 (en) | 2020-09-23 |
| EP3224374A1 (en) | 2017-10-04 |
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