DK3234151T3 - Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf - Google Patents

Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf

Info

Publication number
DK3234151T3
DK3234151T3 DK15823267.8T DK15823267T DK3234151T3 DK 3234151 T3 DK3234151 T3 DK 3234151T3 DK 15823267 T DK15823267 T DK 15823267T DK 3234151 T3 DK3234151 T3 DK 3234151T3
Authority
DK
Denmark
Prior art keywords
genomodification
fungal
systems
methods
fungal genomodification
Prior art date
Application number
DK15823267.8T
Other languages
English (en)
Inventor
Benjamin S Bower
Jimmy Chan
Jing Ge
Xiaogang Gu
Steven Sungjin Kim
Susan Mampusti Madrid
Danfeng Song
Mingmin Song
Michael Ward
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK3234151T3 publication Critical patent/DK3234151T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15823267.8T 2014-12-16 2015-12-16 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf DK3234151T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN2014093916 2014-12-16
CN2014093914 2014-12-16
CN2014093918 2014-12-16
PCT/US2015/066192 WO2016100568A1 (en) 2014-12-16 2015-12-16 Fungal genome modification systems and methods of use

Publications (1)

Publication Number Publication Date
DK3234151T3 true DK3234151T3 (da) 2025-11-03

Family

ID=55083496

Family Applications (3)

Application Number Title Priority Date Filing Date
DK15823254.6T DK3234150T3 (da) 2014-12-16 2015-12-15 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf
DK15823267.8T DK3234151T3 (da) 2014-12-16 2015-12-16 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf
DK15823268.6T DK3234152T3 (da) 2014-12-16 2015-12-16 Svampegenommodificeringssystemer og anvendelsesfremgangsmåder

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK15823254.6T DK3234150T3 (da) 2014-12-16 2015-12-15 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK15823268.6T DK3234152T3 (da) 2014-12-16 2015-12-16 Svampegenommodificeringssystemer og anvendelsesfremgangsmåder

Country Status (12)

Country Link
US (3) US11427829B2 (da)
EP (3) EP3234150B1 (da)
JP (3) JP6814142B2 (da)
KR (3) KR102350405B1 (da)
CN (3) CN107667171A (da)
AU (3) AU2015362784B2 (da)
BR (3) BR112017012837A2 (da)
CA (3) CA2971187C (da)
DK (3) DK3234150T3 (da)
FI (2) FI3234150T3 (da)
MX (3) MX2017007928A (da)
WO (3) WO2016100272A1 (da)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3284749B1 (en) * 2015-04-13 2024-08-14 The University of Tokyo Set of polypeptides exhibiting nuclease activity or nickase activity with dependence on light or in presence of drug or suppressing or activating expression of target gene
WO2017019867A1 (en) * 2015-07-28 2017-02-02 Danisco Us Inc Genome editing systems and methods of use
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US20190359991A1 (en) * 2016-02-04 2019-11-28 Kao Corporation Method for Producing Mutant Filamentous Fungi
DK3419992T3 (da) 2016-02-22 2021-02-15 Danisco Us Inc Svampesystem til proteinproduktion med højt udbytte
WO2017191210A1 (en) * 2016-05-04 2017-11-09 Novozymes A/S Genome editing by crispr-cas9 in filamentous fungal host cells
WO2018015444A1 (en) * 2016-07-22 2018-01-25 Novozymes A/S Crispr-cas9 genome editing with multiple guide rnas in filamentous fungi
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
EP4485466A3 (en) 2016-08-17 2025-04-02 The Broad Institute Inc. Novel crispr enzymes and systems
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US12499971B2 (en) 2016-09-28 2025-12-16 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
US10876103B2 (en) 2016-10-04 2020-12-29 Danisco Us Inc Protein production in filamentous fungal cells in the absence of inducing substrates
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3564358A4 (en) * 2016-12-27 2020-12-16 Kagoshima University SELECTIVE PROTEIN SECRETION TECHNOLOGY IN A MUSHROOM
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018197020A1 (en) * 2017-04-27 2018-11-01 Novozymes A/S Genome editing by crispr-cas9 using short donor oligonucleotides
US11591601B2 (en) 2017-05-05 2023-02-28 The Broad Institute, Inc. Methods for identification and modification of lncRNA associated with target genotypes and phenotypes
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
WO2018226900A2 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
MX2019014640A (es) 2017-06-09 2020-10-05 Editas Medicine Inc Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas.
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019046703A1 (en) * 2017-09-01 2019-03-07 Novozymes A/S METHODS OF ENHANCING GENOME EDITION IN FUNGI
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN108085317B (zh) * 2018-01-17 2019-06-21 四川大学 一种特异性靶向变异链球菌gtfB位点的CRISPR-B基因编辑方法及应用
EP3755710A1 (en) 2018-04-24 2020-12-30 Danisco US Inc. Filamentous fungal strains comprising reduced viscosity phenotypes
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
EP3578658A1 (en) * 2018-06-08 2019-12-11 Johann Wolfgang Goethe-Universität Frankfurt Method for generating a gene editing vector with fixed guide rna pairs
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
JP2021532775A (ja) 2018-07-30 2021-12-02 ダニスコ・ユーエス・インク タンパク質生産性が強化された表現型を含む突然変異及び遺伝子改変糸状菌株並びにそれらの方法
SG11202103917VA (en) 2018-10-16 2021-05-28 Blueallele Llc Methods for targeted insertion of dna in genes
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
EP3874051A1 (en) * 2018-10-31 2021-09-08 Novozymes A/S Genome editing by guided endonuclease and single-stranded oligonucleotide
WO2020123887A2 (en) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
WO2021041922A1 (en) 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
FI4055177T3 (fi) 2019-11-08 2024-10-31 Danisco Us Inc Parantuneen proteiinituottavuuden fenotyyppejä käsittäviä sienikantoja ja menetelmiä niitä varten
KR20230004495A (ko) 2020-04-22 2023-01-06 다니스코 유에스 인크. 사상 진균 세포에서의 개선된 단백질 생산을 위한 조성물 및 방법
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
WO2021242774A1 (en) * 2020-05-26 2021-12-02 Zymergen Inc. Methods of transient protein and gene expression in cells
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
US20240376511A1 (en) 2021-07-19 2024-11-14 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
WO2023039358A1 (en) 2021-09-09 2023-03-16 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production
US20250320512A1 (en) 2021-12-01 2025-10-16 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
CN114410635B (zh) * 2022-03-29 2022-06-14 中国科学院天津工业生物技术研究所 威尼斯镰刀菌内源U6启动子及其基于CRISPR/Cas9的基因编辑方法
AU2023306398A1 (en) 2022-07-15 2025-01-23 International N&H Denmark Aps Improved enzymatic modification of phospholipids in food
EP4615861A1 (en) 2022-11-11 2025-09-17 Danisco US Inc. Filamentous fungal strains comprising enhanced protein productivity phenotypes and methods thereof
EP4632067A4 (en) * 2022-12-08 2026-04-22 Univ Tokyo Science Found METHOD FOR PRODUCING GENOME-MODIFIED CELLS AND METHOD FOR PROMOTING HYBRIDIZATION
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins
WO2024167695A1 (en) 2023-02-08 2024-08-15 Danisco Us Inc. Compositions and methods for producing heterologous globins in filamentous fungal cells
CN121039276A (zh) 2023-03-27 2025-11-28 丹尼斯科美国公司 使用表达来自帽铁形嘉利翁氏菌的RuBisCo的酵母减少发酵甘油和乙酸
KR20250000949A (ko) * 2023-06-26 2025-01-06 전북대학교산학협력단 시그마-54 및 MmoR을 통한 선택적 메탄 모노옥시게나제의 고효율 생산 방법
EP4735472A1 (en) 2023-06-30 2026-05-06 Danisco US Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins
CN116769781B (zh) * 2023-08-16 2023-10-24 中国科学院天津工业生物技术研究所 一种来源于粗壮脉纹孢菌的启动子及其应用
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025207802A1 (en) 2024-03-27 2025-10-02 Nutrition & Biosciences USA 4, Inc. Recombinant microbial strains comprising reduced beta-oxidation phenotypes and methods thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US6436643B1 (en) * 1997-12-22 2002-08-20 Unilever Patent Holdings Bv Process for site-directed integration of multiple copies of a gene in a mould
EP1627049B1 (en) 2003-05-29 2010-02-17 Genencor International, Inc. Novel trichoderma genes
SI1685244T1 (sl) 2003-11-21 2012-06-29 Danisco Us Inc Ekspresija encimov, ki hidrolizirajo zrnat škrob, v Trichodermi in postopek za proizvajanje glukoze iz zrnatih škrobnih substratov
MXPA06013600A (es) 2004-05-27 2007-03-15 Genencor Int Alfa amilasas acidoestables con actividad hidrolizante de almidon granular y composiciones enzimaticas.
CA2801799C (en) 2010-06-03 2018-11-20 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
AU2013335451C1 (en) 2012-10-23 2024-07-04 Toolgen Incorporated Composition for cleaving a target DNA comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof
EP2840140B2 (en) * 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
AU2014273082B2 (en) * 2013-05-29 2018-11-08 Cellectis A method for producing precise DNA cleavage using Cas9 nickase activity
EP3004339B1 (en) * 2013-05-29 2021-07-07 Cellectis New compact scaffold of cas9 in the type ii crispr system
WO2015054507A1 (en) * 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
JP6712948B2 (ja) * 2013-12-12 2020-06-24 ザ・ブロード・インスティテュート・インコーポレイテッド 組成物、及びヌクレオチドリピート障害におけるｃｒｉｓｐｒ−ｃａｓ系の使用方法
US20170088845A1 (en) * 2014-03-14 2017-03-30 The Regents Of The University Of California Vectors and methods for fungal genome engineering by crispr-cas9
SG11201700446XA (en) * 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US10513711B2 (en) * 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
WO2016110453A1 (en) * 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell

Also Published As

Publication number Publication date
AU2015364629A1 (en) 2017-07-06
US11098314B2 (en) 2021-08-24
KR20170089931A (ko) 2017-08-04
US20170369891A1 (en) 2017-12-28
JP2017537647A (ja) 2017-12-21
JP6814141B2 (ja) 2021-01-13
MX2017008036A (es) 2017-09-28
KR102350404B1 (ko) 2022-01-11
WO2016100568A1 (en) 2016-06-23
MX2017007928A (es) 2018-01-30
US20180002710A1 (en) 2018-01-04
AU2015362784A1 (en) 2017-07-06
WO2016100571A1 (en) 2016-06-23
CA2971248A1 (en) 2016-06-23
BR112017012850A2 (pt) 2017-12-26
KR102350405B1 (ko) 2022-01-11
US20190093114A1 (en) 2019-03-28
JP2017538425A (ja) 2017-12-28
FI3234151T3 (fi) 2025-11-05
EP3234151A1 (en) 2017-10-25
DK3234150T3 (da) 2025-11-03
EP3234152B1 (en) 2021-09-15
EP3234151B1 (en) 2025-08-13
EP3234152A1 (en) 2017-10-25
BR112017012851A2 (pt) 2017-12-26
AU2015364632B2 (en) 2021-05-13
JP6814142B2 (ja) 2021-01-13
DK3234152T3 (da) 2021-12-20
EP3234150A1 (en) 2017-10-25
BR112017012837A2 (pt) 2017-12-26
CA2971247A1 (en) 2016-06-23
FI3234150T3 (fi) 2025-11-05
KR20170087521A (ko) 2017-07-28
KR20170087522A (ko) 2017-07-28
CA2971187C (en) 2023-10-24
CN107278231A (zh) 2017-10-20
AU2015364632A1 (en) 2017-07-06
US11427829B2 (en) 2022-08-30
KR102350402B1 (ko) 2022-01-11
EP3234150B1 (en) 2025-08-13
AU2015364629B2 (en) 2021-05-13
CN107667171A (zh) 2018-02-06
MX2017007930A (es) 2018-01-11
AU2015362784B2 (en) 2021-05-13
CA2971187A1 (en) 2016-06-23
WO2016100272A1 (en) 2016-06-23
US11401522B2 (en) 2022-08-02
JP2018504895A (ja) 2018-02-22
CA2971248C (en) 2023-04-04
CN107257859A (zh) 2017-10-17
JP6814143B2 (ja) 2021-01-13

Similar Documents

Publication Publication Date Title
DK3234151T3 (da) Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf
IL259766B (en) Relocalization systems and methods
IL280863A (en) Autotaxin inhibitors and uses thereof
DK3134149T3 (da) Mikrokanyler og fremgangsmåder til fremstilling deraf
DK3201351T3 (da) Kaloriefrie sødestoffer og fremgangsmåder til syntetisering
DK3227336T3 (da) Anti-CD79b-antistoffer og fremgangsmåder til anvendelse
DK3215147T3 (da) Neurodæmpende norketamin-forbindelser og fremgangsmåder
IL248770A0 (en) A trading system
DK3212255T3 (da) Selvorienterende sprøjte og sprøjtegrænseflade
EP3283404C0 (en) STORAGE SYSTEM AND METHODS
EP3283403C0 (en) STORAGE SYSTEMS AND METHODS
DK3265947T3 (da) Fremgangsmåde og system til kryptering
DK3152956T4 (da) Systemer og metoder til kommunikation
DK3213065T3 (da) System og fremgangsmåder til todimensionel rplc-sfc-kromatografi
DK3066591T3 (da) Systemer og fremgangsmåder til billedkarakteristikbaseret genkendelse
DK3218358T3 (da) Ifluorometyl-aminopyridiner og difluorometyl-aminopyrimidiner
DK3227728T3 (da) System og fremgangsmåde til havbundsundersøgelse
DK3194908T3 (da) Overvågningssystemer og fremgangsmåder
DK3116911T3 (da) Anti-mcam-antistoffer og tilhørende fremgangsmåder til anvendelse
IL248781B (en) Pesticidal compositions and related methods
DK3304394T3 (da) Fremgangsmåder og systemer til godkendelse
PL3117909T3 (pl) Urządzenie aplikujące i sposób aplikacji
DK3175588T3 (da) Systemer og fremgangsmåder til beskedredigering
PL3379690T3 (pl) Sposób i system szybkiego ładowania
DK3161139T3 (da) Tlr-4-specifikke aptamerer og anvendelser heraf