DK3234152T3 - Svampegenommodificeringssystemer og anvendelsesfremgangsmåder - Google Patents

Svampegenommodificeringssystemer og anvendelsesfremgangsmåder Download PDF

Info

Publication number
DK3234152T3
DK3234152T3 DK15823268.6T DK15823268T DK3234152T3 DK 3234152 T3 DK3234152 T3 DK 3234152T3 DK 15823268 T DK15823268 T DK 15823268T DK 3234152 T3 DK3234152 T3 DK 3234152T3
Authority
DK
Denmark
Prior art keywords
genomic modification
application procedures
modification systems
fungi
fungi genomic
Prior art date
Application number
DK15823268.6T
Other languages
English (en)
Inventor
Jing Ge
Xiaogang Gu
Susan Mampusti Madrid
Danfeng Song
Mingmin Song
Michael Ward
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK3234152T3 publication Critical patent/DK3234152T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15823268.6T 2014-12-16 2015-12-16 Svampegenommodificeringssystemer og anvendelsesfremgangsmåder DK3234152T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN2014093918 2014-12-16
CN2014093914 2014-12-16
CN2014093916 2014-12-16
PCT/US2015/066195 WO2016100571A1 (en) 2014-12-16 2015-12-16 Fungal genome modification systems and methods of use

Publications (1)

Publication Number Publication Date
DK3234152T3 true DK3234152T3 (da) 2021-12-20

Family

ID=55083496

Family Applications (3)

Application Number Title Priority Date Filing Date
DK15823254.6T DK3234150T3 (da) 2014-12-16 2015-12-15 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf
DK15823268.6T DK3234152T3 (da) 2014-12-16 2015-12-16 Svampegenommodificeringssystemer og anvendelsesfremgangsmåder
DK15823267.8T DK3234151T3 (da) 2014-12-16 2015-12-16 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK15823254.6T DK3234150T3 (da) 2014-12-16 2015-12-15 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK15823267.8T DK3234151T3 (da) 2014-12-16 2015-12-16 Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf

Country Status (12)

Country Link
US (3) US11427829B2 (da)
EP (3) EP3234150B1 (da)
JP (3) JP6814142B2 (da)
KR (3) KR102350405B1 (da)
CN (3) CN107667171A (da)
AU (3) AU2015362784B2 (da)
BR (3) BR112017012837A2 (da)
CA (3) CA2971187C (da)
DK (3) DK3234150T3 (da)
FI (2) FI3234150T3 (da)
MX (3) MX2017007928A (da)
WO (3) WO2016100272A1 (da)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
JP6892642B2 (ja) 2015-04-13 2021-06-23 国立大学法人 東京大学 光依存的に又は薬物存在下でヌクレアーゼ活性若しくはニッカーゼ活性を示す、又は標的遺伝子の発現を抑制若しくは活性化するポリペプチドのセット
DK3329001T3 (da) * 2015-07-28 2021-12-20 Danisco Us Inc Genomredigeringssystemer og anvendelsesfremgangsmåder
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
US20190359991A1 (en) * 2016-02-04 2019-11-28 Kao Corporation Method for Producing Mutant Filamentous Fungi
US10072267B2 (en) 2016-02-22 2018-09-11 Danisco Us Inc Fungal high-level protein production system
WO2017191210A1 (en) * 2016-05-04 2017-11-09 Novozymes A/S Genome editing by crispr-cas9 in filamentous fungal host cells
WO2018015444A1 (en) * 2016-07-22 2018-01-25 Novozymes A/S Crispr-cas9 genome editing with multiple guide rnas in filamentous fungi
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018035388A1 (en) 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018064208A1 (en) 2016-09-28 2018-04-05 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
KR102593668B1 (ko) 2016-10-04 2023-10-24 다니스코 유에스 인크. 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
JP6839424B2 (ja) * 2016-12-27 2021-03-10 国立大学法人 鹿児島大学 真菌におけるタンパク質の選択的分泌技術
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
CN110431224A (zh) 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018197020A1 (en) * 2017-04-27 2018-11-01 Novozymes A/S Genome editing by crispr-cas9 using short donor oligonucleotides
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
JP7518620B2 (ja) 2017-06-09 2024-07-18 エディタス・メディシン,インコーポレイテッド 操作されたcas9ヌクレアーゼ
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019046703A1 (en) * 2017-09-01 2019-03-07 Novozymes A/S METHODS OF ENHANCING GENOME EDITION IN FUNGI
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN108085317B (zh) * 2018-01-17 2019-06-21 四川大学 一种特异性靶向变异链球菌gtfB位点的CRISPR-B基因编辑方法及应用
US11976266B2 (en) 2018-04-24 2024-05-07 Danisco Us Inc. Filamentous fungal strains comprising reduced viscosity phenotypes
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3810750A4 (en) 2018-06-06 2022-03-23 Zymergen, Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
EP3578658A1 (en) * 2018-06-08 2019-12-11 Johann Wolfgang Goethe-Universität Frankfurt Method for generating a gene editing vector with fixed guide rna pairs
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
US12116605B2 (en) 2018-07-30 2024-10-15 Danisco Us Inc. Mutant and genetically modified filamentous fungal strains comprising enhanced protein productivity phenotypes and methods thereof
IL282290B1 (en) 2018-10-16 2026-04-01 Blueallele Llc Methods for directing the insertion of DNA into genes
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20220010305A1 (en) * 2018-10-31 2022-01-13 Novozymes A/S Genome Editing by Guided Endonuclease and Single-stranded Oligonucleotide
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
WO2020191102A1 (en) 2019-03-18 2020-09-24 The Broad Institute, Inc. Type vii crispr proteins and systems
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
WO2021041922A1 (en) 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
EP4055177B1 (en) 2019-11-08 2024-07-31 Danisco US Inc. Fungal strains comprising enhanced protein productivity phenotypes and methods thereof
JP7799621B2 (ja) 2020-04-22 2026-01-15 ダニスコ・ユーエス・インク 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
WO2021242774A1 (en) * 2020-05-26 2021-12-02 Zymergen Inc. Methods of transient protein and gene expression in cells
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN117716039A (zh) 2021-07-19 2024-03-15 丹尼斯科美国公司 用于增强真菌细胞中的蛋白质产生的组合物和方法
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
US20240376512A1 (en) 2021-09-09 2024-11-14 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production
KR20240110854A (ko) 2021-12-01 2024-07-16 다니스코 유에스 인크. 진균 세포에서의 향상된 단백질 생산을 위한 조성물 및 방법
CN114410635B (zh) * 2022-03-29 2022-06-14 中国科学院天津工业生物技术研究所 威尼斯镰刀菌内源U6启动子及其基于CRISPR/Cas9的基因编辑方法
PE20251180A1 (es) 2022-07-15 2025-04-23 Int Nandh Denmark Aps Modificacion enzimatica mejorada de fosfolipidos en alimentos
KR20250102093A (ko) 2022-11-11 2025-07-04 다니스코 유에스 인크. 향상된 단백질 생산성 표현형을 포함하는 사상 진균 균주 및 이의 방법
JPWO2024122646A1 (da) * 2022-12-08 2024-06-13
EP4638700A2 (en) 2022-12-19 2025-10-29 Danisco US Inc. Recombinant fungal strains and methods thereof for producing consistent proteins
JP2026505371A (ja) 2023-02-08 2026-02-13 ダニスコ・ユーエス・インク 糸状真菌細胞において異種グロビンを産生するための組成物及び方法
CN121039276A (zh) 2023-03-27 2025-11-28 丹尼斯科美国公司 使用表达来自帽铁形嘉利翁氏菌的RuBisCo的酵母减少发酵甘油和乙酸
KR20250000949A (ko) * 2023-06-26 2025-01-06 전북대학교산학협력단 시그마-54 및 MmoR을 통한 선택적 메탄 모노옥시게나제의 고효율 생산 방법
WO2025007098A1 (en) 2023-06-30 2025-01-02 Danisco Us Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins
CN116769781B (zh) * 2023-08-16 2023-10-24 中国科学院天津工业生物技术研究所 一种来源于粗壮脉纹孢菌的启动子及其应用
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025207802A1 (en) 2024-03-27 2025-10-02 Nutrition & Biosciences USA 4, Inc. Recombinant microbial strains comprising reduced beta-oxidation phenotypes and methods thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
EP1040197A1 (en) * 1997-12-22 2000-10-04 Unilever N.V. A process for site-directed integration of multiple copies of a gene in a mould
DK1862539T3 (da) 2003-05-29 2012-05-14 Danisco Us Inc Nye trichodermagener
BRPI0416762A (pt) 2003-11-21 2007-02-27 Genencor Int expressão de enzimas hidrolisantes de amido granular em trinchoderma e processo para produzir glicose a partir de substratos de amido granular
US20080199927A1 (en) 2004-05-27 2008-08-21 Nigel Dunn-Coleman Aspergillus Kawachi Acid-Stable Alpha Amylase and Applications in Granular Starch Hydrolysis
CA2801799C (en) * 2010-06-03 2018-11-20 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
PL4397760T3 (pl) * 2012-10-23 2026-03-30 Toolgen Incorporated Kompozycja do rozszczepiania docelowego dna zawierająca prowadzące rna specyficzne dla docelowego dna i kwas nukleinowy kodujący białko cas lub białko cas oraz jej zastosowanie
CN114634950A (zh) * 2012-12-12 2022-06-17 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
ES2670531T3 (es) * 2013-05-29 2018-05-30 Cellectis S.A. Un método para producir una escisión de ADN precisa utilizando la actividad nickasa de Cas9
EP3004339B1 (en) 2013-05-29 2021-07-07 Cellectis New compact scaffold of cas9 in the type ii crispr system
WO2015054507A1 (en) * 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
BR112016013547A2 (pt) * 2013-12-12 2017-10-03 Broad Inst Inc Composições e métodos de uso de sistemas crispr-cas em distúrbios de repetições de nucleotídeos
WO2015138855A1 (en) * 2014-03-14 2015-09-17 The Regents Of The University Of California Vectors and methods for fungal genome engineering by crispr-cas9
AU2015293949B2 (en) * 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
US10513711B2 (en) * 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
US11396665B2 (en) * 2015-01-06 2022-07-26 Dsm Ip Assets B.V. CRISPR-CAS system for a filamentous fungal host cell

Also Published As

Publication number Publication date
AU2015364629A1 (en) 2017-07-06
EP3234151A1 (en) 2017-10-25
EP3234150A1 (en) 2017-10-25
EP3234150B1 (en) 2025-08-13
KR102350404B1 (ko) 2022-01-11
DK3234150T3 (da) 2025-11-03
AU2015362784B2 (en) 2021-05-13
EP3234151B1 (en) 2025-08-13
US20190093114A1 (en) 2019-03-28
KR102350402B1 (ko) 2022-01-11
EP3234152B1 (en) 2021-09-15
KR102350405B1 (ko) 2022-01-11
MX2017007930A (es) 2018-01-11
MX2017008036A (es) 2017-09-28
US11427829B2 (en) 2022-08-30
FI3234151T3 (fi) 2025-11-05
CN107278231A (zh) 2017-10-20
JP6814141B2 (ja) 2021-01-13
CN107257859A (zh) 2017-10-17
FI3234150T3 (fi) 2025-11-05
AU2015362784A1 (en) 2017-07-06
JP2018504895A (ja) 2018-02-22
WO2016100272A1 (en) 2016-06-23
BR112017012850A2 (pt) 2017-12-26
AU2015364632A1 (en) 2017-07-06
JP6814143B2 (ja) 2021-01-13
AU2015364632B2 (en) 2021-05-13
CA2971248C (en) 2023-04-04
BR112017012837A2 (pt) 2017-12-26
JP2017537647A (ja) 2017-12-21
BR112017012851A2 (pt) 2017-12-26
KR20170087522A (ko) 2017-07-28
CA2971247A1 (en) 2016-06-23
CA2971187A1 (en) 2016-06-23
CN107667171A (zh) 2018-02-06
EP3234152A1 (en) 2017-10-25
MX2017007928A (es) 2018-01-30
WO2016100571A1 (en) 2016-06-23
CA2971187C (en) 2023-10-24
US11401522B2 (en) 2022-08-02
KR20170087521A (ko) 2017-07-28
US11098314B2 (en) 2021-08-24
US20170369891A1 (en) 2017-12-28
JP6814142B2 (ja) 2021-01-13
JP2017538425A (ja) 2017-12-28
AU2015364629B2 (en) 2021-05-13
CA2971248A1 (en) 2016-06-23
WO2016100568A1 (en) 2016-06-23
KR20170089931A (ko) 2017-08-04
DK3234151T3 (da) 2025-11-03
US20180002710A1 (en) 2018-01-04

Similar Documents

Publication Publication Date Title
NO2024008I1 (no) Pirtobrutinib
IL259766A (en) Relocalization systems and methods
IL258437B (en) Growing systems and methods
DK3374291T3 (da) Plukningssystemer og -fremgangsmåder
DK3234151T3 (da) Systemer til svampegenommodificering og fremgangsmåder til anvendelse deraf
IL246597A0 (en) Spectrometry systems, methods, and applications
DK3194908T3 (da) Overvågningssystemer og fremgangsmåder
DK3218358T3 (da) Ifluorometyl-aminopyridiner og difluorometyl-aminopyrimidiner
EP4135127C0 (en) MICRO-ASSEMBLY STRATEGIES AND DEVICES FOR COMPOUNDS
GB201720065D0 (en) Systems and methods
DK3215147T3 (da) Neurodæmpende norketamin-forbindelser og fremgangsmåder
DK3103715T3 (da) Styreindretning og styringsfremgangsmåde dertil
GB201506119D0 (en) Systems and methods
DK3234160T3 (da) Sammensætninger og fremgangsmåder til hjælper-stamme-medieret svampegenommodifikation
IL248781B (en) Pesticidal compositions and related methods
DK3304394T3 (da) Fremgangsmåder og systemer til godkendelse
DK3256598T3 (da) Svampestammer og anvendelsesfremgangsmåder
DK3209773T3 (da) Prolintolerante tripeptidylpeptidaser og anvendelser deraf
DK3161139T3 (da) Tlr-4-specifikke aptamerer og anvendelser heraf
IL252224A0 (en) Systems and methods for performing electrocardiograms
BR112016027472A2 (pt) Sistemas e métodos de comunicação
DK3265641T3 (da) Stigørenhed og fremgangsmåde
EP3332266C0 (en) MONITORING SYSTEMS AND METHODS
DK3359773T3 (da) Datasystemer, anordninger og fremgangsmåder
DK3321279T3 (da) Exenatidmodificeringsmiddel og anvendelse deraf