DK3268024T3 - Rekombinante glut1-adeno-associerede virusvektorkonstrukter og relaterede fremgangsmåder til genoprettelse af glut1-ekspression - Google Patents

Rekombinante glut1-adeno-associerede virusvektorkonstrukter og relaterede fremgangsmåder til genoprettelse af glut1-ekspression Download PDF

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DK3268024T3
DK3268024T3 DK16762530.0T DK16762530T DK3268024T3 DK 3268024 T3 DK3268024 T3 DK 3268024T3 DK 16762530 T DK16762530 T DK 16762530T DK 3268024 T3 DK3268024 T3 DK 3268024T3
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glut1
recovering
associated virus
recombinant
related methods
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DK16762530.0T
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Vivo Darryl De
Umrao Monani
Guangping Gao
Kristin Engelstad
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Univ Columbia
Univ Massachusetts
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    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • A61K48/0058Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/14Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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DK16762530.0T 2015-03-10 2016-03-10 Rekombinante glut1-adeno-associerede virusvektorkonstrukter og relaterede fremgangsmåder til genoprettelse af glut1-ekspression DK3268024T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562130899P 2015-03-10 2015-03-10
PCT/US2016/021810 WO2016145217A1 (en) 2015-03-10 2016-03-10 Recombinant glut1 adeno-associated viral vector constructs and related methods for restoring glut1 expression

Publications (1)

Publication Number Publication Date
DK3268024T3 true DK3268024T3 (da) 2020-12-07

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DK16762530.0T DK3268024T3 (da) 2015-03-10 2016-03-10 Rekombinante glut1-adeno-associerede virusvektorkonstrukter og relaterede fremgangsmåder til genoprettelse af glut1-ekspression

Country Status (18)

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US (2) US20180042991A1 (da)
EP (1) EP3268024B1 (da)
JP (3) JP2018509164A (da)
KR (2) KR20240119152A (da)
CN (1) CN107635575A (da)
AU (1) AU2016229000B2 (da)
CL (1) CL2017002282A1 (da)
CO (1) CO2017010149A2 (da)
DK (1) DK3268024T3 (da)
EA (1) EA036051B1 (da)
ES (1) ES2836258T3 (da)
HU (1) HUE052577T2 (da)
IL (2) IL282053B2 (da)
MX (2) MX2017011615A (da)
PT (1) PT3268024T (da)
SG (1) SG11201707116QA (da)
UA (1) UA128249C2 (da)
WO (1) WO2016145217A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107106689A (zh) 2014-11-05 2017-08-29 沃雅戈治疗公司 用于治疗帕金森病的aadc多核苷酸
JP6996728B2 (ja) * 2016-01-07 2022-01-17 学校法人自治医科大学 グルコーストランスポーター1発現用アデノ随伴ウイルスベクター
CN109641063B (zh) 2016-04-20 2022-11-15 能源环境和技术研究中心O.A., M.P. 用于增强pklr的基因表达的组合物和方法
JOP20190200A1 (ar) 2017-02-28 2019-08-27 Univ Pennsylvania تركيبات نافعة في معالجة ضمور العضل النخاعي
JOP20190269A1 (ar) 2017-06-15 2019-11-20 Voyager Therapeutics Inc بولي نوكليوتيدات aadc لعلاج مرض باركنسون
MX2020003042A (es) * 2017-09-29 2020-11-18 Voyager Therapeutics Inc Rescate del fenotipo neurológico central y periférico de la ataxia de friedreich mediante administración intravenosa.
US12441998B2 (en) 2017-10-12 2025-10-14 The Trustees Of Columbia University In The City Of New York SLC2A1 lncRNA as a biologic and related treatments and methods
SG11202002263TA (en) 2017-10-16 2020-04-29 Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas Lentiviral vectors for delivery of pklr to treat pyruvate kinase deficiency
JP7576754B2 (ja) 2018-04-11 2024-11-01 スペースクラフト セブン リミテッド ライアビリティ カンパニー 幹細胞移植のための組成物および方法
AU2019261646A1 (en) 2018-04-27 2020-11-12 Spacecraft Seven, Llc Gene therapy for CNS degeneration
IL278393B2 (en) * 2018-05-08 2023-11-01 Univ Rutgers AAV compatible polymerization proteins bind to laminin
EP3794126A1 (en) 2018-05-15 2021-03-24 Voyager Therapeutics, Inc. Compositions and methods for the treatment of parkinson's disease
CN113383010B (zh) * 2018-09-28 2025-09-09 沃雅戈治疗公司 具有经工程化改造的启动子的共济蛋白表达构建体及其使用方法
US11162102B2 (en) 2019-05-13 2021-11-02 Dna Twopointo Inc. Modifications of mammalian cells using artificial micro-RNA to alter their properties and the compositions of their products
KR20220079857A (ko) * 2019-09-13 2022-06-14 루트거스, 더 스테이트 유니버시티 오브 뉴 저지 Aav-상용성 라미닌-링커 중합 단백질
IL297741A (en) * 2020-05-01 2022-12-01 Atlas Biotechnology S A Treatment and/or prevention of a disease or syndrome associated with a viral infection
EP4178974A4 (en) * 2020-07-09 2024-10-30 The Board of Trustees of the Leland Stanford Junior University IMMUNE CELLS WITH INCREASED GLYCOLYTIC FLOW
JP7853952B2 (ja) * 2020-08-05 2026-04-30 スペースクラフト セブン リミテッド ライアビリティ カンパニー Glut1発現のためのアデノ随伴ウイルスベクターおよびその使用
IL300263A (en) 2020-08-07 2023-03-01 Spacecraft Seven Llc Plakophilin-2 (PKP2) gene therapy using an AAV vector
US20230313187A1 (en) * 2020-09-04 2023-10-05 Dna Twopointo Inc. Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products
JP2025505662A (ja) * 2022-02-08 2025-02-28 スペースクラフト セブン リミテッド ライアビリティ カンパニー Glut1発現用アデノ随伴ウイルスベクターおよびその使用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5399363A (en) 1991-01-25 1995-03-21 Eastman Kodak Company Surface modified anticancer nanoparticles
US5478745A (en) 1992-12-04 1995-12-26 University Of Pittsburgh Recombinant viral vector system
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
IE80468B1 (en) 1995-04-04 1998-07-29 Elan Corp Plc Controlled release biodegradable nanoparticles containing insulin
US6001650A (en) 1995-08-03 1999-12-14 Avigen, Inc. High-efficiency wild-type-free AAV helper functions
JP2001500015A (ja) 1996-09-06 2001-01-09 トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア T7ポリメラーゼを利用する組換えアデノ随伴ウイルスの誘導可能な製造方法
US6156303A (en) 1997-06-11 2000-12-05 University Of Washington Adeno-associated virus (AAV) isolates and AAV vectors derived therefrom
SG10202108118RA (en) 2001-11-13 2021-08-30 Univ Pennsylvania A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
EP2610265B1 (en) * 2003-05-02 2016-11-16 Centre National de la Recherche Scientifique (C.N.R.S.) Glut-1 as a receptor for HTLV envelopes and its uses
JP2006345703A (ja) * 2003-09-09 2006-12-28 Osaka Industrial Promotion Organization 高発現型汎用哺乳動物宿主細胞
HUE054805T2 (hu) 2003-09-30 2021-09-28 Univ Pennsylvania Adeno-asszociált vírus (AAV) kládok, szekvenciák, az azokat tartalmazó vektorok és alkalmazásaik
EP3085389A1 (en) 2005-04-07 2016-10-26 The Trustees Of The University Of Pennsylvania Method of increasing the function of an aav vector
US20100199362A1 (en) * 2007-04-26 2010-08-05 President And Fellows Of Harvard College Wnt ligands involved in blood-brain barrier development and uses therefor
WO2010071832A1 (en) * 2008-12-19 2010-06-24 Nationwide Children's Hospital Delivery of polynucleotides across the blood brain barrier using recombinant aav9
US9610363B2 (en) * 2009-04-16 2017-04-04 President And Fellows Of Harvard College Methods for inhibiting starvation of a cell
EP3567106A1 (en) * 2010-04-23 2019-11-13 University of Massachusetts Cns targeting aav vectors and methods of use thereof
EP2394667A1 (en) * 2010-06-10 2011-12-14 Laboratorios Del Dr. Esteve, S.A. Vectors and sequences for the treatment of diseases

Also Published As

Publication number Publication date
JP2023144087A (ja) 2023-10-06
HUE052577T2 (hu) 2021-05-28
EP3268024A1 (en) 2018-01-17
WO2016145217A1 (en) 2016-09-15
ES2836258T3 (es) 2021-06-24
UA128249C2 (uk) 2024-05-22
AU2016229000B2 (en) 2021-11-04
SG11201707116QA (en) 2017-09-28
EP3268024A4 (en) 2018-08-01
PT3268024T (pt) 2020-12-09
MX2023001213A (es) 2023-03-03
JP2021097689A (ja) 2021-07-01
EA201791936A1 (ru) 2018-01-31
MX2017011615A (es) 2018-04-11
IL282053A (en) 2021-05-31
CL2017002282A1 (es) 2018-04-13
CO2017010149A2 (es) 2018-02-28
US20210069292A1 (en) 2021-03-11
BR112017019294A2 (pt) 2018-05-02
KR20180016722A (ko) 2018-02-19
EA201791936A8 (ru) 2018-11-30
IL254286B (en) 2021-04-29
NZ735046A (en) 2024-11-29
IL282053B2 (en) 2023-03-01
KR20240119152A (ko) 2024-08-06
CA2978917A1 (en) 2016-09-15
EA036051B1 (ru) 2020-09-18
AU2016229000A1 (en) 2017-09-21
EP3268024B1 (en) 2020-09-09
US20180042991A1 (en) 2018-02-15
CN107635575A (zh) 2018-01-26
IL282053B (en) 2022-11-01
JP2018509164A (ja) 2018-04-05
IL254286A0 (en) 2017-10-31

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