DK3527667T3 - Gærpromotorer til proteinekspression - Google Patents
Gærpromotorer til proteinekspression Download PDFInfo
- Publication number
- DK3527667T3 DK3527667T3 DK19150337.4T DK19150337T DK3527667T3 DK 3527667 T3 DK3527667 T3 DK 3527667T3 DK 19150337 T DK19150337 T DK 19150337T DK 3527667 T3 DK3527667 T3 DK 3527667T3
- Authority
- DK
- Denmark
- Prior art keywords
- protein expression
- yeast promoters
- promoters
- yeast
- protein
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361775029P | 2013-03-08 | 2013-03-08 | |
| EP14760869.9A EP2964775B1 (en) | 2013-03-08 | 2014-03-07 | Yeast promoters for protein expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3527667T3 true DK3527667T3 (da) | 2021-02-22 |
Family
ID=51492025
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14760869.9T DK2964775T3 (da) | 2013-03-08 | 2014-03-07 | Gærpromotorer til proteinekspression |
| DK19150337.4T DK3527667T3 (da) | 2013-03-08 | 2014-03-07 | Gærpromotorer til proteinekspression |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14760869.9T DK2964775T3 (da) | 2013-03-08 | 2014-03-07 | Gærpromotorer til proteinekspression |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US10676750B2 (da) |
| EP (3) | EP3527667B1 (da) |
| BR (1) | BR112015021785A2 (da) |
| CA (1) | CA2904333C (da) |
| DK (2) | DK2964775T3 (da) |
| ES (3) | ES2716619T3 (da) |
| HU (1) | HUE042897T2 (da) |
| PT (1) | PT2964775T (da) |
| WO (1) | WO2014138679A1 (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2964775T3 (da) | 2013-03-08 | 2019-04-15 | Biogrammatics Inc | Gærpromotorer til proteinekspression |
| BR112015021753B1 (pt) | 2013-03-08 | 2023-02-23 | Keck Graduate Institute Of Applied Life Sciences | Ácidos nucleicos isolados de pichia pastoris, vetor de expressão, células hospedeiras de levedura e construto de dna, bem como método para produzir uma proteína |
| WO2016077457A1 (en) | 2014-11-11 | 2016-05-19 | Clara Foods Co. | Methods and compositions for egg white protein production |
| US10745730B2 (en) | 2015-09-28 | 2020-08-18 | The Trustees Of Columbia University Of The City Of New York | Modified bacterial protein expression system |
| CN109536395A (zh) * | 2018-02-07 | 2019-03-29 | 四川润格生物科技有限公司 | 一种高表达β-甘露聚糖酶的毕赤酵母工程菌株及应用 |
| KR102131882B1 (ko) * | 2018-02-14 | 2020-08-06 | 씨제이제일제당 주식회사 | 효모를 이용하여 이취가 개선된 발효 조성물의 제조방법 |
| FI13395Y1 (fi) | 2019-07-11 | 2023-06-06 | Clara Foods Co | Koostumuksia, jotka käsittävät rekombinanttia ovomukoidiproteiinia |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| WO2022115636A1 (en) * | 2020-11-25 | 2022-06-02 | Esperovax Inc. | Cell viability switch for genetically modified organisms |
| WO2023184539A1 (zh) * | 2022-04-02 | 2023-10-05 | 武汉远大弘元股份有限公司 | 一种甲醇诱导启动子及其在制备丙谷二肽中的应用 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US14715A (en) * | 1856-04-22 | Improvement in cultivators | ||
| US14716A (en) * | 1856-04-22 | Machine for digging peat | ||
| US7078035B2 (en) | 1997-08-13 | 2006-07-18 | Diversa Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
| US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| MXPA01009809A (es) | 1999-03-31 | 2002-07-30 | Cornell Res Foundation Inc | Fosfatasas con actividad de fitasa mejorada. |
| US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
| GB0126164D0 (en) | 2001-10-31 | 2002-01-02 | Danisco | Sequences |
| WO2003037102A2 (en) | 2001-10-31 | 2003-05-08 | Phytex, Llc | Phytase-containing animal food and method |
| MY139056A (en) | 2001-12-28 | 2009-08-28 | Ab Enzymes Gmbh | Microbially-expressed thermotolerant phytase for animal feed |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US7943360B2 (en) | 2002-04-19 | 2011-05-17 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| CN101014704A (zh) | 2004-06-21 | 2007-08-08 | 诺维信公司 | 蛋白酶 |
| AT501955B1 (de) | 2005-02-23 | 2007-08-15 | Univ Graz Tech | Mutierte aox1-promotoren |
| ATE486944T1 (de) * | 2005-11-16 | 2010-11-15 | Korea Res Inst Of Bioscience | Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon |
| DK2365064T3 (da) * | 2006-04-04 | 2015-03-30 | Novozymes As | Phytasevarianter |
| CN101460612B (zh) | 2006-04-04 | 2015-05-06 | 诺维信公司 | 肌醇六磷酸酶变体 |
| WO2008063302A2 (en) | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| US8143023B2 (en) | 2007-01-26 | 2012-03-27 | Novozymes A/S | Method for methanol independent induction from methanol inducible promoters in Pichia |
| JP2010524440A (ja) | 2007-04-20 | 2010-07-22 | ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング | 発現系 |
| US20110129874A1 (en) * | 2008-07-11 | 2011-06-02 | Novozymes A/S | Pichia Pastoris Das Promoter Variants |
| EP2401378B1 (en) * | 2009-02-25 | 2013-08-14 | Merck Sharp & Dohme Corp. | Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris |
| WO2012129036A2 (en) * | 2011-03-22 | 2012-09-27 | Merck Sharp & Dohme Corp. | Promoters for high level recombinant expression in fungal host cells |
| CN102994501B (zh) | 2012-10-15 | 2018-04-27 | 华南理工大学 | 具有组成型启动子活性的dna及其应用和巴斯德毕赤酵母表达载体 |
| DK2964775T3 (da) | 2013-03-08 | 2019-04-15 | Biogrammatics Inc | Gærpromotorer til proteinekspression |
| BR112015021753B1 (pt) | 2013-03-08 | 2023-02-23 | Keck Graduate Institute Of Applied Life Sciences | Ácidos nucleicos isolados de pichia pastoris, vetor de expressão, células hospedeiras de levedura e construto de dna, bem como método para produzir uma proteína |
| US9150870B2 (en) | 2013-03-15 | 2015-10-06 | Lonza Ltd. | Constitutive promoter |
| US9822357B2 (en) * | 2013-07-05 | 2017-11-21 | Technische Universitat Graz | Bidirectional promoter |
| WO2019038338A1 (en) * | 2017-08-23 | 2019-02-28 | Sandoz Ag | PROTEIN EXPRESSION SYSTEM WITH MULTIPLE GENIC COPIES |
-
2014
- 2014-03-07 DK DK14760869.9T patent/DK2964775T3/da active
- 2014-03-07 CA CA2904333A patent/CA2904333C/en active Active
- 2014-03-07 EP EP19150337.4A patent/EP3527667B1/en active Active
- 2014-03-07 HU HUE14760869A patent/HUE042897T2/hu unknown
- 2014-03-07 WO PCT/US2014/022086 patent/WO2014138679A1/en not_active Ceased
- 2014-03-07 EP EP20209215.1A patent/EP3839055B1/en active Active
- 2014-03-07 ES ES14760869T patent/ES2716619T3/es active Active
- 2014-03-07 ES ES20209215T patent/ES2961268T3/es active Active
- 2014-03-07 US US14/773,482 patent/US10676750B2/en active Active
- 2014-03-07 PT PT14760869T patent/PT2964775T/pt unknown
- 2014-03-07 EP EP14760869.9A patent/EP2964775B1/en active Active
- 2014-03-07 ES ES19150337T patent/ES2856265T3/es active Active
- 2014-03-07 DK DK19150337.4T patent/DK3527667T3/da active
- 2014-03-07 BR BR112015021785A patent/BR112015021785A2/pt not_active Application Discontinuation
-
2020
- 2020-04-29 US US16/861,384 patent/US11555194B2/en active Active
-
2023
- 2023-01-04 US US18/149,872 patent/US12264319B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2716619T3 (es) | 2019-06-13 |
| PT2964775T (pt) | 2019-05-06 |
| EP2964775A4 (en) | 2017-03-01 |
| EP3839055A3 (en) | 2021-10-20 |
| WO2014138679A1 (en) | 2014-09-12 |
| EP2964775A1 (en) | 2016-01-13 |
| CN105358706A (zh) | 2016-02-24 |
| EP3839055B1 (en) | 2023-08-23 |
| ES2856265T3 (es) | 2021-09-27 |
| DK2964775T3 (da) | 2019-04-15 |
| US12264319B2 (en) | 2025-04-01 |
| US11555194B2 (en) | 2023-01-17 |
| CA2904333A1 (en) | 2014-09-12 |
| EP3527667B1 (en) | 2020-11-25 |
| CA2904333C (en) | 2024-06-25 |
| EP3527667A2 (en) | 2019-08-21 |
| EP2964775B1 (en) | 2019-01-09 |
| US10676750B2 (en) | 2020-06-09 |
| US20230340507A1 (en) | 2023-10-26 |
| EP3839055A2 (en) | 2021-06-23 |
| EP3839055C0 (en) | 2023-08-23 |
| HUE042897T2 (hu) | 2019-07-29 |
| US20160024511A1 (en) | 2016-01-28 |
| US20210032643A1 (en) | 2021-02-04 |
| EP3527667A3 (en) | 2019-10-02 |
| BR112015021785A2 (pt) | 2017-08-29 |
| ES2961268T3 (es) | 2024-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL284301A (en) | Heterodimeric proteins | |
| DK2970441T3 (da) | AGLYCOSYLEREDE Fc-INDEHOLDENDE POLYPEPTIDER | |
| DK3336106T3 (da) | Anti-fcrh5-antistoffer | |
| DK3708583T3 (da) | Ikke-fucosylerede anti-fgfr2iiib-antistoffer | |
| HUE042897T2 (hu) | Élesztõpromóterek fehérje-expresszióhoz | |
| DK3041857T3 (da) | Protein a-kromatografi | |
| DK2992104T3 (da) | Fremgangsmåde til ekspression | |
| HRP20190434T1 (hr) | Fuzijski protein | |
| DK3068800T3 (da) | FcRn-specifikke antistoffer | |
| PL2968470T3 (pl) | Zmodyfikowane białka stanowiące substancję hamującą rozwój przewodów müllera (mis) i ich zastosowania do leczenia chorób | |
| DK2943561T3 (da) | Fotobioreaktor | |
| CL2016001195A1 (es) | Promotores sinteticos | |
| DK3262168T3 (da) | Værtscelleproteinmodifikation | |
| DK3184642T3 (da) | Gærcelle | |
| EP2970925A4 (en) | Phytase | |
| DK3259358T3 (da) | Forøget proteinekspression | |
| DK3431504T3 (da) | Antiphospholipase d4-antistof | |
| HUE050362T2 (hu) | Javított rekombináns fehérje expresszió hibrid CHEF1 promótert alkalmazva | |
| LT3030259T (lt) | Peptidai | |
| DK3065774T3 (da) | Anti-CCL17-antistoffer | |
| HUE047455T2 (hu) | HPA-1A elleni antitestek | |
| DK3047028T3 (da) | Fermenteringsfremgangsmåde | |
| EP2804946A4 (en) | RECOMBINANT CHO GMT PROTEIN EXPRESSION | |
| DK3089990T3 (da) | Forbedret proteinekspression | |
| DK3089991T3 (da) | Forbedret proteinekspression |