DK3628732T3 - Transposasesammensætninger til reduktion af insertionsbias - Google Patents
Transposasesammensætninger til reduktion af insertionsbias Download PDFInfo
- Publication number
- DK3628732T3 DK3628732T3 DK19187628.3T DK19187628T DK3628732T3 DK 3628732 T3 DK3628732 T3 DK 3628732T3 DK 19187628 T DK19187628 T DK 19187628T DK 3628732 T3 DK3628732 T3 DK 3628732T3
- Authority
- DK
- Denmark
- Prior art keywords
- reducing insertion
- insertion bias
- transposase
- compositions
- transposase compositions
- Prior art date
Links
- 102000008579 Transposases Human genes 0.000 title 1
- 108010020764 Transposases Proteins 0.000 title 1
- 230000037431 insertion Effects 0.000 title 1
- 238000003780 insertion Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Non-Portable Lighting Devices Or Systems Thereof (AREA)
- Endoscopes (AREA)
- Fittings On The Vehicle Exterior For Carrying Loads, And Devices For Holding Or Mounting Articles (AREA)
- Enzymes And Modification Thereof (AREA)
- Fuel-Injection Apparatus (AREA)
- Mechanical Coupling Of Light Guides (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462075713P | 2014-11-05 | 2014-11-05 | |
| US201562242935P | 2015-10-16 | 2015-10-16 | |
| EP15798597.9A EP3215618B1 (en) | 2014-11-05 | 2015-11-05 | Transposase compositions for reduction of insertion bias |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3628732T3 true DK3628732T3 (da) | 2023-05-30 |
Family
ID=54697644
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15798597.9T DK3215618T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
| DK19187628.3T DK3628732T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15798597.9T DK3215618T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US10815478B2 (da) |
| EP (3) | EP3628732B1 (da) |
| CN (1) | CN107532164A (da) |
| AU (2) | AU2015343034B2 (da) |
| CA (1) | CA2965995A1 (da) |
| DK (2) | DK3215618T3 (da) |
| ES (1) | ES2947757T3 (da) |
| FI (1) | FI3628732T3 (da) |
| SG (1) | SG11201703472YA (da) |
| WO (1) | WO2016073690A1 (da) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| DK3215618T3 (da) * | 2014-11-05 | 2019-10-14 | Illumina Inc | Transposasesammensætninger til reduktion af insertionsbias |
| WO2016115550A1 (en) | 2015-01-16 | 2016-07-21 | Seqwell, Inc. | Normalized iterative barcoding and sequencing of dna collections |
| CA3007840C (en) | 2015-12-07 | 2020-09-15 | Zymergen Inc. | Microbial strain improvement by a htp genomic engineering platform |
| CA3007635A1 (en) | 2015-12-07 | 2017-06-15 | Zymergen Inc. | Promoters from corynebacterium glutamicum |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| WO2018005793A1 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
| JP2019519242A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | 細菌ヘモグロビンライブラリーを生成するための方法およびその使用 |
| EP3497219A4 (en) * | 2016-08-10 | 2020-08-19 | President and Fellows of Harvard College | NOVO ASSEMBLY PROCESSES FROM GENOMIC DNA BARCODE FRAGMENTS |
| CA3048863A1 (en) | 2016-12-29 | 2018-07-05 | Illumina, Inc. | Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments |
| KR20200010285A (ko) | 2017-05-19 | 2020-01-30 | 지머젠 인코포레이티드 | 증가된 nadph를 유도하는 생합성 경로의 게놈 공학 |
| JP7350659B2 (ja) | 2017-06-06 | 2023-09-26 | ザイマージェン インコーポレイテッド | Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａ ｓｐｉｎｏｓａの改良のためのハイスループット（ＨＴＰ）ゲノム操作プラットフォーム |
| KR20200026878A (ko) | 2017-06-06 | 2020-03-11 | 지머젠 인코포레이티드 | 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼 |
| KR20200026874A (ko) | 2017-06-06 | 2020-03-11 | 지머젠 인코포레이티드 | 대장균 개량을 위한 htp 게놈 공학 플랫폼 |
| EP3635111A1 (en) | 2017-06-06 | 2020-04-15 | Zymergen, Inc. | High throughput transposon mutagenesis |
| CA3091228A1 (en) | 2018-03-20 | 2019-09-26 | Zymergen Inc. | A htp platform for the genetic engineering of chinese hamster ovary cells |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| BR112021006038A2 (pt) | 2019-01-11 | 2021-10-26 | Illumina Cambridge Limited | Complexos de stranspossomas ligados à superfície do complexo |
| CN113366115B (zh) | 2019-01-29 | 2024-07-05 | 深圳华大智造科技股份有限公司 | 高覆盖率stlfr |
| AU2020312787A1 (en) | 2019-07-12 | 2021-06-17 | Illumina Cambridge Limited | Nucleic acid library preparation using electrophoresis |
| US11377655B2 (en) | 2019-07-16 | 2022-07-05 | Pacific Biosciences Of California, Inc. | Synthetic nucleic acids having non-natural structures |
| US20220396788A1 (en) * | 2019-09-12 | 2022-12-15 | Thermo Fisher Scientific Baltics, UAB | Recombinant transposon ends |
| JP7489455B2 (ja) | 2019-10-25 | 2024-05-23 | チャンピン ナショナル ラボラトリー | 哺乳類ｄｎａのメチル化の検出及び分析 |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| EP4103580A4 (en) | 2020-02-13 | 2024-03-06 | Zymergen Inc. | METAGENOMIC LIBRARY AND NATURAL PRODUCT DISCOVERY PLATFORM |
| JP2025521678A (ja) | 2022-06-30 | 2025-07-10 | カパ バイオシステムズ，インコーポレイティド | 古細菌ヒストン様タンパク質を使用したタグメンテーション配列決定ライブラリーインサートサイズの制御 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| FI103809B1 (fi) * | 1997-07-14 | 1999-09-30 | Finnzymes Oy | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten |
| CN1181852C (zh) * | 2002-05-21 | 2004-12-29 | 崔行善 | 一种治疗膀胱癌的药物 |
| FR2900935A1 (fr) * | 2006-05-15 | 2007-11-16 | Centre Nat Rech Scient | Systemes de transposition recombinants hyperactifs derives du transposon mos-1 |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| ES2743029T3 (es) | 2008-10-24 | 2020-02-18 | Epicentre Tech Corporation | Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos |
| EP2635679B1 (en) * | 2010-11-05 | 2017-04-19 | Illumina, Inc. | Linking sequence reads using paired code tags |
| WO2012108864A1 (en) | 2011-02-08 | 2012-08-16 | Illumina, Inc. | Selective enrichment of nucleic acids |
| US9005935B2 (en) * | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| US20130143774A1 (en) | 2011-12-05 | 2013-06-06 | The Regents Of The University Of California | Methods and compositions for generating polynucleic acid fragments |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014205296A1 (en) * | 2013-06-21 | 2014-12-24 | The Broad Institute, Inc. | Methods for shearing and tagging dna for chromatin immunoprecipitation and sequencing |
| WO2015089339A2 (en) * | 2013-12-11 | 2015-06-18 | Changping Shi | Compositions, methods and kits for dna fragmentation and tagmentation |
| CA3262557A1 (en) * | 2014-04-15 | 2026-03-02 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
| DK3215618T3 (da) * | 2014-11-05 | 2019-10-14 | Illumina Inc | Transposasesammensætninger til reduktion af insertionsbias |
-
2015
- 2015-11-05 DK DK15798597.9T patent/DK3215618T3/da active
- 2015-11-05 FI FIEP19187628.3T patent/FI3628732T3/fi active
- 2015-11-05 US US15/523,765 patent/US10815478B2/en active Active
- 2015-11-05 AU AU2015343034A patent/AU2015343034B2/en active Active
- 2015-11-05 ES ES19187628T patent/ES2947757T3/es active Active
- 2015-11-05 WO PCT/US2015/059194 patent/WO2016073690A1/en not_active Ceased
- 2015-11-05 CN CN201580060259.8A patent/CN107532164A/zh active Pending
- 2015-11-05 DK DK19187628.3T patent/DK3628732T3/da active
- 2015-11-05 EP EP19187628.3A patent/EP3628732B1/en active Active
- 2015-11-05 SG SG11201703472YA patent/SG11201703472YA/en unknown
- 2015-11-05 EP EP23170515.3A patent/EP4239067A3/en active Pending
- 2015-11-05 EP EP15798597.9A patent/EP3215618B1/en active Active
- 2015-11-05 CA CA2965995A patent/CA2965995A1/en active Pending
-
2020
- 2020-09-02 US US17/010,349 patent/US12180468B2/en active Active
-
2022
- 2022-02-23 AU AU2022201248A patent/AU2022201248B2/en active Active
-
2024
- 2024-11-19 US US18/952,092 patent/US20250145985A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3215618B1 (en) | 2019-07-24 |
| WO2016073690A1 (en) | 2016-05-12 |
| AU2015343034B2 (en) | 2021-12-16 |
| FI3628732T3 (fi) | 2023-05-31 |
| US20180016571A1 (en) | 2018-01-18 |
| EP4239067A2 (en) | 2023-09-06 |
| US20210047635A1 (en) | 2021-02-18 |
| SG11201703472YA (en) | 2017-05-30 |
| DK3215618T3 (da) | 2019-10-14 |
| US12180468B2 (en) | 2024-12-31 |
| EP3628732A1 (en) | 2020-04-01 |
| AU2015343034A1 (en) | 2017-05-18 |
| AU2015343034A9 (en) | 2019-08-01 |
| CN107532164A (zh) | 2018-01-02 |
| WO2016073690A9 (en) | 2017-09-14 |
| EP3628732B1 (en) | 2023-05-03 |
| CA2965995A1 (en) | 2016-05-12 |
| AU2022201248B2 (en) | 2025-01-09 |
| AU2022201248A1 (en) | 2022-03-17 |
| US20250145985A1 (en) | 2025-05-08 |
| US10815478B2 (en) | 2020-10-27 |
| EP3215618A1 (en) | 2017-09-13 |
| ES2947757T3 (es) | 2023-08-18 |
| EP4239067A3 (en) | 2023-10-25 |
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