DK3628732T3 - Transposasesammensætninger til reduktion af insertionsbias - Google Patents
Transposasesammensætninger til reduktion af insertionsbias Download PDFInfo
- Publication number
- DK3628732T3 DK3628732T3 DK19187628.3T DK19187628T DK3628732T3 DK 3628732 T3 DK3628732 T3 DK 3628732T3 DK 19187628 T DK19187628 T DK 19187628T DK 3628732 T3 DK3628732 T3 DK 3628732T3
- Authority
- DK
- Denmark
- Prior art keywords
- reducing insertion
- insertion bias
- transposase
- compositions
- transposase compositions
- Prior art date
Links
- 102000008579 Transposases Human genes 0.000 title 1
- 108010020764 Transposases Proteins 0.000 title 1
- 230000037431 insertion Effects 0.000 title 1
- 238000003780 insertion Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Non-Portable Lighting Devices Or Systems Thereof (AREA)
- Endoscopes (AREA)
- Fittings On The Vehicle Exterior For Carrying Loads, And Devices For Holding Or Mounting Articles (AREA)
- Enzymes And Modification Thereof (AREA)
- Fuel-Injection Apparatus (AREA)
- Mechanical Coupling Of Light Guides (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462075713P | 2014-11-05 | 2014-11-05 | |
| US201562242935P | 2015-10-16 | 2015-10-16 | |
| EP15798597.9A EP3215618B1 (en) | 2014-11-05 | 2015-11-05 | Transposase compositions for reduction of insertion bias |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3628732T3 true DK3628732T3 (da) | 2023-05-30 |
Family
ID=54697644
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK19187628.3T DK3628732T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
| DK15798597.9T DK3215618T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15798597.9T DK3215618T3 (da) | 2014-11-05 | 2015-11-05 | Transposasesammensætninger til reduktion af insertionsbias |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US10815478B2 (da) |
| EP (3) | EP4239067A3 (da) |
| CN (1) | CN107532164A (da) |
| AU (2) | AU2015343034B2 (da) |
| CA (1) | CA2965995A1 (da) |
| DK (2) | DK3628732T3 (da) |
| ES (1) | ES2947757T3 (da) |
| FI (1) | FI3628732T3 (da) |
| SG (1) | SG11201703472YA (da) |
| WO (1) | WO2016073690A1 (da) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US10815478B2 (en) * | 2014-11-05 | 2020-10-27 | Illumina, Inc. | Method of sequential tagmentation with transposase compositions for reduction of insertion bias |
| EP3245304B1 (en) | 2015-01-16 | 2021-06-30 | Seqwell, Inc. | Normalized iterative barcoding and sequencing of dna collections |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| BR112018011503A2 (pt) | 2015-12-07 | 2018-12-04 | Zymergen Inc | promotores da corynebacterium glutamicum |
| KR20190090081A (ko) | 2015-12-07 | 2019-07-31 | 지머젠 인코포레이티드 | Htp 게놈 공학 플랫폼에 의한 미생물 균주 개량 |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| EP3478845A4 (en) | 2016-06-30 | 2019-07-31 | Zymergen, Inc. | METHOD OF GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF |
| US10544390B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| AU2017311306A1 (en) * | 2016-08-10 | 2019-03-14 | President And Fellows Of Harvard College | Methods of de novo assembly of barcoded genomic DNA fragments |
| JP7234114B2 (ja) * | 2016-12-29 | 2023-03-07 | イルミナ インコーポレイテッド | 細胞区画内の生体分子への直交アクセスおよび細胞区画内の生体分子のタグ付けのための分析システム |
| CA3061731A1 (en) | 2017-05-19 | 2018-11-22 | Zymergen Inc. | Genomic engineering of biosynthetic pathways leading to increased nadph |
| WO2018226810A1 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | High throughput transposon mutagenesis |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
| WO2018226880A1 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving escherichia coli |
| WO2018226893A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa |
| WO2019183183A1 (en) | 2018-03-20 | 2019-09-26 | Zymergen Inc. | A htp platform for the genetic engineering of chinese hamster ovary cells |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| IL281664B2 (en) | 2019-01-11 | 2024-07-01 | Illumina Cambridge Ltd | Complex surface-bound transposome complexes |
| EP3918088B1 (en) | 2019-01-29 | 2024-03-13 | MGI Tech Co., Ltd. | High coverage stlfr |
| AU2020312787A1 (en) * | 2019-07-12 | 2021-06-17 | Illumina Cambridge Limited | Nucleic acid library preparation using electrophoresis |
| US11377655B2 (en) | 2019-07-16 | 2022-07-05 | Pacific Biosciences Of California, Inc. | Synthetic nucleic acids having non-natural structures |
| US20220396788A1 (en) * | 2019-09-12 | 2022-12-15 | Thermo Fisher Scientific Baltics, UAB | Recombinant transposon ends |
| US12553044B2 (en) | 2019-10-25 | 2026-02-17 | Changping National Laboratory | Methylation detection and analysis of mammalian DNA |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| JP2023513314A (ja) | 2020-02-13 | 2023-03-30 | ザイマージェン インコーポレイテッド | メタゲノムライブラリーおよび天然物発見プラットフォーム |
| EP4547839A1 (en) * | 2022-06-30 | 2025-05-07 | Kapa Biosystems, Inc. | Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| FI103809B1 (fi) * | 1997-07-14 | 1999-09-30 | Finnzymes Oy | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten |
| CN1181852C (zh) * | 2002-05-21 | 2004-12-29 | 崔行善 | 一种治疗膀胱癌的药物 |
| FR2900935A1 (fr) * | 2006-05-15 | 2007-11-16 | Centre Nat Rech Scient | Systemes de transposition recombinants hyperactifs derives du transposon mos-1 |
| CA2750054C (en) | 2008-10-24 | 2018-05-29 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2012061832A1 (en) * | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9365897B2 (en) | 2011-02-08 | 2016-06-14 | Illumina, Inc. | Selective enrichment of nucleic acids |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| WO2013085918A1 (en) | 2011-12-05 | 2013-06-13 | The Regents Of The University Of California | Methods and compostions for generating polynucleic acid fragments |
| US9644199B2 (en) * | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US20160208323A1 (en) | 2013-06-21 | 2016-07-21 | The Broad Institute, Inc. | Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing |
| WO2015089339A2 (en) * | 2013-12-11 | 2015-06-18 | Changping Shi | Compositions, methods and kits for dna fragmentation and tagmentation |
| WO2015160895A2 (en) * | 2014-04-15 | 2015-10-22 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
| US10815478B2 (en) * | 2014-11-05 | 2020-10-27 | Illumina, Inc. | Method of sequential tagmentation with transposase compositions for reduction of insertion bias |
-
2015
- 2015-11-05 US US15/523,765 patent/US10815478B2/en active Active
- 2015-11-05 SG SG11201703472YA patent/SG11201703472YA/en unknown
- 2015-11-05 DK DK19187628.3T patent/DK3628732T3/da active
- 2015-11-05 ES ES19187628T patent/ES2947757T3/es active Active
- 2015-11-05 WO PCT/US2015/059194 patent/WO2016073690A1/en not_active Ceased
- 2015-11-05 EP EP23170515.3A patent/EP4239067A3/en active Pending
- 2015-11-05 EP EP19187628.3A patent/EP3628732B1/en active Active
- 2015-11-05 FI FIEP19187628.3T patent/FI3628732T3/fi active
- 2015-11-05 AU AU2015343034A patent/AU2015343034B2/en active Active
- 2015-11-05 CA CA2965995A patent/CA2965995A1/en active Pending
- 2015-11-05 EP EP15798597.9A patent/EP3215618B1/en active Active
- 2015-11-05 CN CN201580060259.8A patent/CN107532164A/zh active Pending
- 2015-11-05 DK DK15798597.9T patent/DK3215618T3/da active
-
2020
- 2020-09-02 US US17/010,349 patent/US12180468B2/en active Active
-
2022
- 2022-02-23 AU AU2022201248A patent/AU2022201248B2/en active Active
-
2024
- 2024-11-19 US US18/952,092 patent/US20250145985A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016073690A1 (en) | 2016-05-12 |
| DK3215618T3 (da) | 2019-10-14 |
| WO2016073690A9 (en) | 2017-09-14 |
| EP4239067A2 (en) | 2023-09-06 |
| AU2022201248B2 (en) | 2025-01-09 |
| US20180016571A1 (en) | 2018-01-18 |
| US20250145985A1 (en) | 2025-05-08 |
| FI3628732T3 (fi) | 2023-05-31 |
| EP3628732B1 (en) | 2023-05-03 |
| EP4239067A3 (en) | 2023-10-25 |
| US10815478B2 (en) | 2020-10-27 |
| CA2965995A1 (en) | 2016-05-12 |
| SG11201703472YA (en) | 2017-05-30 |
| AU2015343034B2 (en) | 2021-12-16 |
| ES2947757T3 (es) | 2023-08-18 |
| EP3215618A1 (en) | 2017-09-13 |
| AU2022201248A1 (en) | 2022-03-17 |
| EP3215618B1 (en) | 2019-07-24 |
| EP3628732A1 (en) | 2020-04-01 |
| CN107532164A (zh) | 2018-01-02 |
| US12180468B2 (en) | 2024-12-31 |
| AU2015343034A1 (en) | 2017-05-18 |
| AU2015343034A9 (en) | 2019-08-01 |
| US20210047635A1 (en) | 2021-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3628732T3 (da) | Transposasesammensætninger til reduktion af insertionsbias | |
| EP3534968A4 (en) | ANTI-BCMA T CAR LYMPHOCYTE COMPOSITIONS | |
| DK3116533T3 (da) | Deletion af dystrophingenexon under anvendelse af genmanipulerede nukleaser | |
| DK3102576T3 (da) | Dihydropyrrolopyridininhibitorer af ror-gamma | |
| DK3137169T3 (da) | Hæmmere af lysin-specifik demethylase-1 | |
| DK3192902T3 (da) | Fremgangsmåde til fremstilling af graphenholdige viscosefibre | |
| DK3092256T3 (da) | Forbindelser og sammensætninger til immunterapi | |
| DK3757214T3 (da) | Sammensætninger til modulering af sod-1-ekspression | |
| DK3536380T3 (da) | Hurtigtvirkende insulinsammensætninger | |
| DK3215173T3 (da) | Glycopeptidsammensætninger | |
| DK3164380T3 (da) | Hæmmere af lysinspecifik demethylase-1 | |
| DK3294700T3 (da) | Fremgangsmåde til fremstilling af limonen-4-ol | |
| DK3233108T3 (da) | Hurtigtvirkende insulinsammensætninger | |
| DK3250182T3 (da) | Dispergible sammensætninger | |
| DK3116475T3 (da) | Dopa-decarboxylasehæmmersammensætninger | |
| DK3334726T3 (da) | Plinabulinsammensætninger | |
| MA42692A (fr) | Compositions biopharmaceutiques | |
| DK3220891T3 (da) | Sublingual formulering af riluzol | |
| DK3143026T3 (da) | Forbindelser og sammensætninger til induktion af chondrogenese | |
| DK3229995T3 (da) | Fremgangsmåde for fremstilling af et endestykke | |
| EP3325533A4 (en) | HARDENABLE BENZOXAZINE COMPOSITIONS | |
| DK3240767T3 (da) | Mikroindkapslede nitrifikationshæmmersammensætninger | |
| EP3388505A4 (en) | surfactant | |
| DK3204352T3 (da) | Hæmmere af lysin-gingipain | |
| EP3388504A4 (en) | surfactant |