DK3684924T5 - Ikke-integrerende dna-vektorer til genetisk modifikation af celler - Google Patents

Ikke-integrerende dna-vektorer til genetisk modifikation af celler Download PDF

Info

Publication number: DK3684924T5
Authority: DK; Denmark
Prior art keywords: cells; genetic modification; dna vectors; integrating dna; integrating
Prior art date: 2017-09-19

Application number

DK18769377.5T

Other languages

English (en)

Other versions

DK3684924T3 (da

Inventor

Richard Harbottle

Matthias Bozza

James A Williams

Original Assignee

Deutsches Krebsforsch

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2017-09-19

Filing date

2018-09-19

Publication date

2024-08-26

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=60037350&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3684924(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

2018-09-19 Application filed by Deutsches Krebsforsch filed Critical Deutsches Krebsforsch

2023-02-06 Application granted granted Critical

2023-02-06 Publication of DK3684924T3 publication Critical patent/DK3684924T3/da

2024-08-26 Publication of DK3684924T5 publication Critical patent/DK3684924T5/da

Links

238000012239 gene modification Methods 0.000 title 1
230000005017 genetic modification Effects 0.000 title 1
235000013617 genetically modified food Nutrition 0.000 title 1
239000013598 vector Substances 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Cell Biology (AREA)
Hematology (AREA)
Medicinal Chemistry (AREA)
Pharmacology & Pharmacy (AREA)
Epidemiology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Developmental Biology & Embryology (AREA)
Virology (AREA)

DK18769377.5T 2017-09-19 2018-09-19 Ikke-integrerende dna-vektorer til genetisk modifikation af celler DK3684924T5 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP17191829.5A EP3456821B2 (en)	2017-09-19	2017-09-19	Non-integrating dna vectors for the genetic modification of cells
PCT/EP2018/075353 WO2019057773A1 (en)	2017-09-19	2018-09-19	NON-INTEGRATED DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS

Publications (2)

Publication Number	Publication Date
DK3684924T3 DK3684924T3 (da)	2023-02-06
DK3684924T5 true DK3684924T5 (da)	2024-08-26

Family

ID=60037350

Family Applications (3)

Application Number	Title	Priority Date	Filing Date
DK17191829.5T DK3456821T4 (da)	2017-09-19	2017-09-19	Ikke-integrerende dna-vektorer til genetisk modifikation af celler
DK18769377.5T DK3684924T5 (da)	2017-09-19	2018-09-19	Ikke-integrerende dna-vektorer til genetisk modifikation af celler
DK18769378.3T DK3684925T3 (da)	2017-09-19	2018-09-19	Ikke-integrerende dna-vektorer til genetisk modifikation af celler

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
DK17191829.5T DK3456821T4 (da)	2017-09-19	2017-09-19	Ikke-integrerende dna-vektorer til genetisk modifikation af celler

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
DK18769378.3T DK3684925T3 (da)	2017-09-19	2018-09-19	Ikke-integrerende dna-vektorer til genetisk modifikation af celler

Country Status (18)

Country	Link
US (3)	US12215335B2 (da)
EP (4)	EP3456821B2 (da)
JP (3)	JP7189943B2 (da)
KR (2)	KR102714302B1 (da)
CN (2)	CN111278972B (da)
AU (3)	AU2018337672B2 (da)
CA (2)	CA3017658A1 (da)
DK (3)	DK3456821T4 (da)
ES (3)	ES2821655T5 (da)
FI (2)	FI3456821T4 (da)
HU (1)	HUE060893T2 (da)
IL (2)	IL273267B2 (da)
LT (1)	LT3684924T (da)
MX (1)	MX2020003092A (da)
PL (1)	PL3684924T3 (da)
PT (1)	PT3684924T (da)
SG (2)	SG11202002367WA (da)
WO (2)	WO2019060253A1 (da)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US10704021B2 (en)	2012-03-15	2020-07-07	Flodesign Sonics, Inc.	Acoustic perfusion devices
WO2015105955A1 (en)	2014-01-08	2015-07-16	Flodesign Sonics, Inc.	Acoustophoresis device with dual acoustophoretic chamber
US11377651B2 (en)	2016-10-19	2022-07-05	Flodesign Sonics, Inc.	Cell therapy processes utilizing acoustophoresis
US11708572B2 (en)	2015-04-29	2023-07-25	Flodesign Sonics, Inc.	Acoustic cell separation techniques and processes
WO2019118921A1 (en)	2017-12-14	2019-06-20	Flodesign Sonics, Inc.	Acoustic transducer drive and controller
CN114423863A (zh) *	2019-03-18	2022-04-29	德国癌症研究中心	用于细胞遗传修饰的表达构建体
GB2700048B (en)	2019-09-18	2026-03-04	Aldevron Llc	synthetic dna vectors and method of use
JP2023537858A (ja) *	2020-07-22	2023-09-06	ドイチェスクレブスフォルシュンクスツェントルム	５’ｓ／ｍａｒの適用
CN113025651B (zh) *	2021-03-31	2023-03-24	重庆医科大学	靶向HBV核心启动子的药物筛选细胞模型、Triciribine及结构类似物新应用
CA3223292A1 (en)	2021-06-25	2022-12-29	Laura Van Lieshout	Adeno-associated virus packaging systems
EP4430077A1 (en)	2021-11-11	2024-09-18	Patrick Schmidt	Immunoreactive molecules and uses thereof
WO2023083982A1 (en)	2021-11-11	2023-05-19	Universitaet Heidelberg	Immunoreactive molecules and uses thereof
WO2023114901A2 (en)	2021-12-15	2023-06-22	Oxford Biomedica Solutions Llc	Methods and compositions for the production of adeno-associated virus
EP4590841A2 (en) *	2022-09-20	2025-07-30	Aldevron, LLC	Best1 vectors and uses thereof
CN115896172A (zh) *	2022-09-29	2023-04-04	深圳盛源生物技术有限公司	一种快速建立果蝇细胞稳定表达株的共转染载体及其制备方法和应用
WO2026071760A1 (ko) *	2024-09-27	2026-04-02	주식회사 엘지화학	Cho 세포 유래 s/mar 및 이를 이용한 발현 벡터

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5888774A (en)	1994-12-19	1999-03-30	Cangene Corporation	Recombinant DNA molecules and expression vectors for erythropoietin
FR2738842B1 (fr)	1995-09-15	1997-10-31	Rhone Poulenc Rorer Sa	Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US5922583A (en)	1995-10-17	1999-07-13	Biostar Inc.	Methods for production of recombinant plasmids
DE19848017A1 (de)	1998-10-17	2000-04-20	Multigene Biotech Gmbh	Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen
FR2821855B1 (fr)	2001-03-09	2004-04-02	Cayla	Genes synthetiques et plasmides bacteriens depourvus de cpg
WO2005005644A1 (en) *	2003-07-11	2005-01-20	Cytos Biotechnology Ag	Gene expression system
WO2005040377A2 (en) *	2003-10-24	2005-05-06	Selexis S.A.	High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
GB0327056D0 (en)	2003-11-20	2003-12-24	Cobra Biolog Ltd	Plasmid maintenance
WO2005100604A2 (en)	2004-04-01	2005-10-27	Sequenom, Inc.	Methods for identifying risk of osteoarthritis and treatments thereof
TWI311152B (en)	2004-09-17	2009-06-21	Boehringer Ingelheim Rcv Gmbh & Co K	Host-vector system for antibiotic-free cole1 plasmid propagation
EP1815021A2 (en)	2004-11-03	2007-08-08	Almac Diagnostics Limited	Transcriptome microarray technology and methods of using the same
WO2007039920A1 (en)	2005-10-05	2007-04-12	Universita' Degli Studi Di Milano-Bicocca	A method for the transfer of episomal vectors into animal cells
GB2439935B (en)	2006-07-08	2011-09-14	Lyndex Recycling Systems Ltd	Compacting and Bailing Apparatus
EP2152889B1 (en)	2007-05-29	2017-03-15	Nature Technology Corporation	Vectors and methods for genetic immunization
WO2010006415A1 (en)	2008-06-30	2010-01-21	Atgcell Inc.	Mammalian cell expression vectors and utilization
EP3093351B1 (en) *	2008-07-09	2018-04-18	Celera Corporation	Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof
WO2010018444A2 (en)	2008-08-12	2010-02-18	Avesthagen Limited	An expression vector and a method thereof
US9045759B2 (en)	2009-01-21	2015-06-02	James Arthur Williams	DNA plasmids with improved copy number
WO2012046255A2 (en)	2010-10-08	2012-04-12	Cadila Healthcare Limited	Expression vector for high level expression of recombinant proteins
EP2476755A1 (en)	2011-01-17	2012-07-18	Helmholtz-Zentrum für Infektionsforschung GmbH	S/MAR containing expression vectors, cell and cell lines as well as methods using the same
GB201213117D0 (en)	2012-07-24	2012-09-05	Ucl Business Plc	Transgene expression
US9550998B2 (en) *	2012-08-29	2017-01-24	Nature Technology Corporation	DNA plasmids with improved expression
US20150322439A1 (en)	2012-11-19	2015-11-12	Nature Technology Corporation	Replicative minicircle vectors with improved expression
EP2878674A1 (en)	2013-11-28	2015-06-03	Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC)	Stable episomes based on non-integrative lentiviral vectors
JP6544565B2 (ja)	2015-04-28	2019-07-17	国立大学法人広島大学	哺乳動物細胞内で目的遺伝子の発現を高める方法およびキット、並びに、その利用
CN114423863A (zh) *	2019-03-18	2022-04-29	德国癌症研究中心	用于细胞遗传修饰的表达构建体

2017
- 2017-09-19 EP EP17191829.5A patent/EP3456821B2/en active Active
- 2017-09-19 FI FIEP17191829.5T patent/FI3456821T4/fi active
- 2017-09-19 DK DK17191829.5T patent/DK3456821T4/da active
- 2017-09-19 ES ES17191829T patent/ES2821655T5/es active Active
2018
- 2018-09-17 US US16/648,885 patent/US12215335B2/en active Active
- 2018-09-17 WO PCT/US2018/051381 patent/WO2019060253A1/en not_active Ceased
- 2018-09-18 CA CA3017658A patent/CA3017658A1/en active Pending
- 2018-09-19 ES ES18769378T patent/ES2887776T3/es active Active
- 2018-09-19 AU AU2018337672A patent/AU2018337672B2/en active Active
- 2018-09-19 PL PL18769377.5T patent/PL3684924T3/pl unknown
- 2018-09-19 MX MX2020003092A patent/MX2020003092A/es unknown
- 2018-09-19 US US16/648,768 patent/US20210403941A1/en active Pending
- 2018-09-19 LT LTEPPCT/EP2018/075353T patent/LT3684924T/lt unknown
- 2018-09-19 CN CN201880061036.7A patent/CN111278972B/zh active Active
- 2018-09-19 DK DK18769377.5T patent/DK3684924T5/da active
- 2018-09-19 AU AU2018337673A patent/AU2018337673B2/en active Active
- 2018-09-19 CN CN201880061037.1A patent/CN111492056B/zh active Active
- 2018-09-19 JP JP2020516915A patent/JP7189943B2/ja active Active
- 2018-09-19 DK DK18769378.3T patent/DK3684925T3/da active
- 2018-09-19 IL IL273267A patent/IL273267B2/en unknown
- 2018-09-19 JP JP2020516608A patent/JP7263327B2/ja active Active
- 2018-09-19 FI FIEP18769377.5T patent/FI3684924T3/fi active
- 2018-09-19 SG SG11202002367WA patent/SG11202002367WA/en unknown
- 2018-09-19 EP EP22201555.4A patent/EP4186975A1/en active Pending
- 2018-09-19 CA CA3076285A patent/CA3076285A1/en active Pending
- 2018-09-19 PT PT187693775T patent/PT3684924T/pt unknown
- 2018-09-19 EP EP18769377.5A patent/EP3684924B1/en active Active
- 2018-09-19 US US16/649,064 patent/US20200263195A1/en not_active Abandoned
- 2018-09-19 KR KR1020207010722A patent/KR102714302B1/ko active Active
- 2018-09-19 SG SG11202001941SA patent/SG11202001941SA/en unknown
- 2018-09-19 EP EP18769378.3A patent/EP3684925B1/en active Active
- 2018-09-19 ES ES18769377T patent/ES2942210T3/es active Active
- 2018-09-19 KR KR1020207010863A patent/KR102711571B1/ko active Active
- 2018-09-19 WO PCT/EP2018/075353 patent/WO2019057773A1/en not_active Ceased
- 2018-09-19 HU HUE18769377A patent/HUE060893T2/hu unknown
2020
- 2020-03-17 IL IL273362A patent/IL273362A/en unknown
2023
- 2023-04-12 JP JP2023064820A patent/JP7721589B2/ja active Active
2025
- 2025-05-26 AU AU2025203887A patent/AU2025203887A1/en active Pending

Also Published As

Publication number	Publication date
KR20200079244A (ko)	2020-07-02
ES2821655T3 (es)	2021-04-27
ES2942210T3 (es)	2023-05-30
IL273267A (en)	2020-04-30
IL273267B1 (en)	2024-11-01
EP3456821B2 (en)	2024-01-24
EP4186975A1 (en)	2023-05-31
JP2023089132A (ja)	2023-06-27
ES2821655T5 (es)	2024-08-06
PT3684924T (pt)	2023-02-03
EP3456821B1 (en)	2020-08-12
IL273362A (en)	2020-05-31
CN111278972A (zh)	2020-06-12
JP2020535805A (ja)	2020-12-10
US12215335B2 (en)	2025-02-04
CN111492056A (zh)	2020-08-04
FI3684924T3 (fi)	2023-02-22
KR20200054278A (ko)	2020-05-19
AU2018337672B2 (en)	2025-02-27
LT3684924T (lt)	2023-02-10
SG11202001941SA (en)	2020-04-29
US20200277624A1 (en)	2020-09-03
AU2018337673B2 (en)	2025-04-17
CA3076285A1 (en)	2019-03-28
EP3456821A1 (en)	2019-03-20
EP3684924A1 (en)	2020-07-29
ES2887776T3 (es)	2021-12-27
DK3456821T3 (da)	2020-11-16
CA3017658A1 (en)	2019-03-19
SG11202002367WA (en)	2020-04-29
JP7189943B2 (ja)	2022-12-14
IL273267B2 (en)	2025-03-01
EP3684925B1 (en)	2021-08-18
WO2019057773A1 (en)	2019-03-28
JP7721589B2 (ja)	2025-08-12
PL3684924T3 (pl)	2023-04-17
CN111492056B (zh)	2024-06-21
FI3456821T4 (fi)	2024-04-24
AU2018337672A1 (en)	2020-03-19
US20200263195A1 (en)	2020-08-20
AU2018337673A1 (en)	2020-04-02
US20210403941A1 (en)	2021-12-30
KR102711571B1 (ko)	2024-09-27
DK3684925T3 (da)	2021-11-22
JP7263327B2 (ja)	2023-04-24
MX2020003092A (es)	2020-07-28
KR102714302B1 (ko)	2024-10-07
EP3684925A1 (en)	2020-07-29
CN111278972B (zh)	2024-04-19
DK3684924T3 (da)	2023-02-06
HUE060893T2 (hu)	2023-04-28
JP2020536510A (ja)	2020-12-17
AU2025203887A1 (en)	2025-06-19
WO2019060253A1 (en)	2019-03-28
EP3684924B1 (en)	2022-11-02
DK3456821T4 (da)	2024-04-15

Publication	Publication Date	Title
DK3684925T3 (da)	2021-11-22	Ikke-integrerende dna-vektorer til genetisk modifikation af celler
EP3755799A4 (en)	2021-12-08	LYSIS BY FREEZE-DEFROST OF A SINGLE CELL
DK3277805T3 (da)	2025-06-10	CAS-9-retrovirale integrasesystemer til målrettet inkorporering af en dna-sekvens i en celles genom
DK3981884T3 (da)	2023-09-04	Enkeltcelle-helgenombiblioteker til methyleringssekventering
DK3417073T3 (da)	2023-10-30	Genetiske markører til transplantation af humane, hjerteventrikulære progenitorceller
IL257009A (en)	2018-03-29	Cells for immunotherapy engineered for targeting cd38 antigen and for cd38 gene inactivation
DK3448990T3 (da)	2021-08-30	Forbedrede fremgangsmåder til modificering af målnukleinsyrer
DK3294325T5 (da)	2024-10-07	Forbedring af virkningen af car-manipulerede t-celler ved hjælp af nukleinsyrevaccination
DK3515600T3 (da)	2023-08-07	Array af organoider
DK3390429T3 (da)	2025-08-25	Modificerede kapsid-proteiner til forbedret afgivelse af parvovirus-vektorer
DK3512959T3 (da)	2021-10-18	Fremgangsmåder til udførelse af multipleks PCR
DK3500664T3 (da)	2021-12-06	Fremgangsmåde til differentiering af pluripotente celler
DK3299923T3 (da)	2020-05-25	Fremgangsmåde til at styre gåstilling af tobenet robot og indretning
DK3444334T3 (da)	2021-11-08	Fremgangsmåde til inducering af CD8+ T-celler
DK3563156T3 (da)	2021-11-08	Fremgangsmåde til separering af biomolekyler
DK3317400T3 (da)	2021-01-18	Fremgangsmåde til kryokonservering af tumorinfiltrerende lymfocytter
DK3213084T4 (da)	2024-03-18	Fremgangsmåde til berigelse af cirkulerende tumor dna
DK3288405T3 (da)	2021-07-26	Kemisk minimeret system til tidsreduceret påsætning af øjenvippeforlængelser
DK3287523T3 (da)	2022-07-25	Fremgangsmåde til sekretorisk fremstilling af protein
DK3460102T3 (da)	2020-07-13	Fremgangsmåde til fremstilling af en elektrokatalysator
DK3455625T3 (da)	2020-10-26	Fremgangsmåder til forudsigelse af anvendeligheden af neo-antigener til immunterapi
DK3242931T3 (da)	2021-05-10	Formuleringer til stabilisering af bakteriecelleekstrakter
DK3224356T3 (da)	2020-07-13	Metoder til rekombinant udtrykkelse af beta-glucosidase-gen
DK3331986T3 (da)	2020-08-31	Fremgangsmåde til fremstilling af celledyrkningsmedium
DK3724319T3 (da)	2023-05-01	Fremgangsmåder til opnåelse af muskelafledte celler