DK3957744T5 - Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer - Google Patents
Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer Download PDFInfo
- Publication number
- DK3957744T5 DK3957744T5 DK21168174.7T DK21168174T DK3957744T5 DK 3957744 T5 DK3957744 T5 DK 3957744T5 DK 21168174 T DK21168174 T DK 21168174T DK 3957744 T5 DK3957744 T5 DK 3957744T5
- Authority
- DK
- Denmark
- Prior art keywords
- reagents
- methods
- nucleic acids
- single cell
- cell nucleic
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1622222.6A GB201622222D0 (en) | 2016-12-23 | 2016-12-23 | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| EP17817859.6A EP3559267B1 (en) | 2016-12-23 | 2017-12-19 | Method for molecular barcoding of nucleic acids of single cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3957744T3 DK3957744T3 (da) | 2023-09-18 |
| DK3957744T5 true DK3957744T5 (da) | 2024-07-15 |
Family
ID=58360450
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17817859.6T DK3559267T3 (da) | 2016-12-23 | 2017-12-19 | Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller |
| DK21168174.7T DK3957744T5 (da) | 2016-12-23 | 2017-12-19 | Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer |
| DK23181491.4T DK4265723T3 (da) | 2016-12-23 | 2017-12-19 | Reagenser og fremgangsmåder til molekylær stregkodning af nukleotidsyrer fra enkeltceller |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17817859.6T DK3559267T3 (da) | 2016-12-23 | 2017-12-19 | Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK23181491.4T DK4265723T3 (da) | 2016-12-23 | 2017-12-19 | Reagenser og fremgangsmåder til molekylær stregkodning af nukleotidsyrer fra enkeltceller |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US12084714B2 (da) |
| EP (4) | EP3957744B1 (da) |
| DK (3) | DK3559267T3 (da) |
| ES (3) | ES2880394T3 (da) |
| GB (1) | GB201622222D0 (da) |
| HR (1) | HRP20211078T1 (da) |
| SI (1) | SI3559267T1 (da) |
| WO (1) | WO2018115852A1 (da) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| SG10201801654RA (en) | 2014-05-28 | 2018-04-27 | Childrens Hospital Med Ct | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| AU2015331848B2 (en) | 2014-10-17 | 2022-03-03 | Children's Hospital Medical Center, D/B/A Cincinnati Children's Hospital Medical Center | In vivo model of human small intestine using pluripotent stem cells and methods of making and using same |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| CN109415685B (zh) | 2016-05-05 | 2023-07-04 | 儿童医院医疗中心 | 用于体外制造胃底组织的方法和与其相关的组合物 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| EP4553082A3 (en) | 2016-11-04 | 2025-08-20 | Children's Hospital Medical Center | Liver organoid compositions and methods of making and using same |
| EP3548507A4 (en) | 2016-12-05 | 2020-07-15 | Children's Hospital Medical Center | COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF |
| GB201622222D0 (en) * | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| JP7248586B2 (ja) | 2017-04-14 | 2023-03-29 | チルドレンズ ホスピタル メディカル センター | 複数ドナー幹細胞組成物およびそれを作製する方法 |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| EP3694603B1 (en) | 2017-10-10 | 2026-04-08 | Children's Hospital Medical Center | Esophageal tissue and/or organoid compositions and methods of making same |
| EP3727394A4 (en) | 2017-12-21 | 2021-09-08 | Children's Hospital Medical Center | DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| EP4678727A3 (en) | 2018-07-26 | 2026-03-11 | Children's Hospital Medical Center | Hepato-biliary-pancreatic tissues and methods of making same |
| CA3112026A1 (en) | 2018-09-12 | 2020-03-19 | Children's Hospital Medical Center | Organoid compositions for the production of hematopoietic stem cells and derivatives thereof |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| US12600943B2 (en) | 2019-02-01 | 2026-04-14 | The University Of Hong Kong | Innervated organoid compositions and methods of making same |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| BR112021006234A2 (pt) * | 2019-03-01 | 2021-09-28 | Illumina, Inc. | Bibliotecas de células únicas e núcleos únicos de alto rendimento e métodos de preparo e uso |
| US12497597B2 (en) | 2019-05-31 | 2025-12-16 | Children's Hospital Medical Center | Methods of generating and expanding hematopoietic stem cells |
| AU2020283048A1 (en) | 2019-05-31 | 2021-12-23 | Children's Hospital Medical Center | Shaped organoid compositions and methods of making same |
| WO2020243647A1 (en) * | 2019-05-31 | 2020-12-03 | Children’S Hospital Medical Center | Polymer based cellular labeling, barcoding and assembly |
| GB201909325D0 (en) | 2019-06-28 | 2019-08-14 | Cs Genetics Ltd | Reagents and methods for analysis for microparticles |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| CN112280829B (zh) * | 2020-04-07 | 2023-12-26 | 南方科技大学 | 一种试剂盒、样本标记方法、单细胞测序方法 |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| WO2022051296A1 (en) * | 2020-09-01 | 2022-03-10 | Ultima Genomics, Inc. | Methods and systems for reducing particle aggregation |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| WO2022263846A1 (en) | 2021-06-18 | 2022-12-22 | Cs Genetics Limited | Reagents and methods for molecular barcoding |
| US20240084378A1 (en) | 2022-05-11 | 2024-03-14 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
| WO2024173380A2 (en) * | 2023-02-13 | 2024-08-22 | The Regents Of The University Of California | Method for acquiring cellular spatial information using light-sensitive dna barcodes |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060040286A1 (en) | 2001-03-28 | 2006-02-23 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| WO2005001113A2 (en) | 2003-06-27 | 2005-01-06 | Thomas Jefferson University | Methods for detecting nucleic acid variations |
| US20050064435A1 (en) | 2003-09-24 | 2005-03-24 | Xing Su | Programmable molecular barcodes |
| EP3029141A1 (en) | 2009-08-20 | 2016-06-08 | Population Genetics Technologies Ltd. | Compositions and methods for intramolecular nucleic acid rearrangement |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| WO2016100976A2 (en) * | 2014-12-19 | 2016-06-23 | Apprise Bio, Inc. | Methods for identifying multiple epitopes in selected sub-populations of cells |
| WO2012112804A1 (en) * | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| ES2986436T3 (es) | 2011-04-15 | 2024-11-11 | Univ Johns Hopkins | Sistema de secuenciación segura |
| AU2012304328B2 (en) | 2011-09-09 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| WO2014071361A1 (en) | 2012-11-05 | 2014-05-08 | Rubicon Genomics | Barcoding nucleic acids |
| WO2014141386A1 (ja) | 2013-03-12 | 2014-09-18 | 株式会社日立製作所 | 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置 |
| US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| US10428373B2 (en) * | 2013-11-15 | 2019-10-01 | The Regents Of The University Of California | Duplicating DNA with contiguity barcodes for genome and epigenome sequencing |
| GB201322692D0 (en) | 2013-12-20 | 2014-02-05 | Philochem Ag | Production of encoded chemical libraries |
| US10526601B2 (en) | 2014-05-23 | 2020-01-07 | Digenomix Corporation | Haploidome determination by digitized transposons |
| WO2015200893A2 (en) * | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US10246701B2 (en) | 2014-11-14 | 2019-04-02 | Adaptive Biotechnologies Corp. | Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture |
| WO2016145416A2 (en) | 2015-03-11 | 2016-09-15 | The Broad Institute, Inc. | Proteomic analysis with nucleic acid identifiers |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US11661597B2 (en) * | 2015-04-15 | 2023-05-30 | The Board Of Trustees Of The Leland Stanford Junior University | Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes |
| US11746367B2 (en) | 2015-04-17 | 2023-09-05 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| GB2539675B (en) * | 2015-06-23 | 2017-11-22 | Cs Genetics Ltd | Libraries of multimeric barcoding reagents and kits thereof for labelling nucleic acids for sequencing |
| US9768159B2 (en) * | 2015-08-19 | 2017-09-19 | Texas Instruments Incorporated | Electrostatic discharge protection device for high voltage |
| MX2018003532A (es) * | 2015-09-24 | 2019-04-25 | Abvitro Llc | Conjugados de afinidad-oligonucleotido y usos de los mismos. |
| US10870879B2 (en) | 2015-10-05 | 2020-12-22 | Helmholtz Zentrum Münchendeutsches Forschungszentrum Für Gesundheit Und Umwelt | Method for the preparation of bar-coded primer sets |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| CA3062248A1 (en) | 2017-05-05 | 2018-11-08 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
-
2016
- 2016-12-23 GB GBGB1622222.6A patent/GB201622222D0/en not_active Ceased
-
2017
- 2017-12-19 WO PCT/GB2017/053816 patent/WO2018115852A1/en not_active Ceased
- 2017-12-19 ES ES17817859T patent/ES2880394T3/es active Active
- 2017-12-19 ES ES23181491T patent/ES3058149T3/es active Active
- 2017-12-19 DK DK17817859.6T patent/DK3559267T3/da active
- 2017-12-19 EP EP21168174.7A patent/EP3957744B1/en active Active
- 2017-12-19 US US16/471,941 patent/US12084714B2/en active Active
- 2017-12-19 HR HRP20211078TT patent/HRP20211078T1/hr unknown
- 2017-12-19 EP EP17817859.6A patent/EP3559267B1/en active Active
- 2017-12-19 ES ES21168174T patent/ES2955997T3/es active Active
- 2017-12-19 DK DK21168174.7T patent/DK3957744T5/da active
- 2017-12-19 EP EP25198641.0A patent/EP4667567A3/en active Pending
- 2017-12-19 DK DK23181491.4T patent/DK4265723T3/da active
- 2017-12-19 EP EP23181491.4A patent/EP4265723B1/en active Active
- 2017-12-19 SI SI201730847T patent/SI3559267T1/sl unknown
-
2023
- 2023-03-17 US US18/185,904 patent/US11845924B1/en active Active
- 2023-11-06 US US18/502,977 patent/US20240327896A1/en active Pending
-
2024
- 2024-08-02 US US18/793,425 patent/US20250207176A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3559267B1 (en) | 2021-04-14 |
| EP4667567A2 (en) | 2025-12-24 |
| EP3559267A1 (en) | 2019-10-30 |
| HRP20211078T1 (hr) | 2021-10-01 |
| ES3058149T3 (en) | 2026-03-09 |
| EP4667567A3 (en) | 2026-03-18 |
| ES2880394T3 (es) | 2021-11-24 |
| EP3957744A1 (en) | 2022-02-23 |
| US20240327896A1 (en) | 2024-10-03 |
| EP4265723A3 (en) | 2024-01-10 |
| GB201622222D0 (en) | 2017-02-08 |
| EP3957744B1 (en) | 2023-06-28 |
| US12084714B2 (en) | 2024-09-10 |
| EP4265723A2 (en) | 2023-10-25 |
| DK3957744T3 (da) | 2023-09-18 |
| WO2018115852A1 (en) | 2018-06-28 |
| DK3559267T3 (da) | 2021-07-12 |
| ES2955997T3 (es) | 2023-12-11 |
| SI3559267T1 (sl) | 2021-08-31 |
| US11845924B1 (en) | 2023-12-19 |
| DK4265723T3 (da) | 2026-01-12 |
| US20190316182A1 (en) | 2019-10-17 |
| US20250207176A1 (en) | 2025-06-26 |
| EP4265723B1 (en) | 2025-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3957744T3 (da) | Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer | |
| IL283416A (en) | Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis | |
| DK3615690T3 (da) | Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker | |
| DK3615671T3 (da) | Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker | |
| DK3408407T3 (da) | Fremgangsmåder til påvisning af cpg-methylering af tumorafledt dna i blodprøver | |
| EP3784799C0 (en) | METHOD FOR DETECTING NUCLEIC ACIDS | |
| DK3334841T3 (da) | Fremgangsmåde til undersøgelse af nukleinsyrer | |
| DK3882357T3 (da) | Fremgangsmåder og sammensætninger til analyse af nukleinsyrer | |
| DK3615691T3 (da) | Sammensætninger og fremgangsmåder til forbedring af prøveidentifikation i indekserede nukleinsyrebiblioteker | |
| DK3471749T3 (da) | Fremgangsmåder og sammensætninger til detektering af en mål-RNA | |
| DK3386550T3 (da) | Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer | |
| DK3115457T3 (da) | Fremgangsmåde til modificering af genomsekvens til specifikt at konvertere nukleinsyrebaser af målrettet dna-sekvens og molekylært kompleks til anvendelse i samme | |
| DK3456846T3 (da) | Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre | |
| DK3448990T3 (da) | Forbedrede fremgangsmåder til modificering af målnukleinsyrer | |
| DK3272867T3 (da) | Anvendelse af programmerbare dna-bindingsproteiner til forbedring af målrettet genommodifikation | |
| DK3218511T3 (da) | Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre | |
| DK3114225T3 (da) | Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver | |
| DK3436469T3 (da) | Nukleinsyrestabiliseringsreagens, kits og fremgangsmåder til anvendelse deraf | |
| DK3572527T3 (da) | Fremgangsmåder og præparater til fremstilling af nukleinsyrebiblioteker | |
| EP3371194A4 (en) | PROTEIN SEQUENCING METHOD AND REAGENTS | |
| DK3122897T3 (da) | Detektering af målnukleinsyresekvenser under anvendelse af forskellige detekteringstemperaturer | |
| DK3411151T3 (da) | Mikrofluidt assaysystem og fremgangsmåde til at udføre et assay | |
| DK3377653T3 (da) | Fremgangsmåder til opnåelse og korrektion af biologisk sekvensinformation | |
| DK3298162T3 (da) | Detektering af målnukleinsyre og varianter | |
| EP3683579C0 (en) | METHODS FOR THE DETECTION OF HOST CELL PROTEINS |