EA200901331A1 - Способ определения по крайней мере одной нерастворимой мишени в биологическом образце (варианты) - Google Patents

Способ определения по крайней мере одной нерастворимой мишени в биологическом образце (варианты)

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Publication number
EA200901331A1
EA200901331A1 EA200901331A EA200901331A EA200901331A1 EA 200901331 A1 EA200901331 A1 EA 200901331A1 EA 200901331 A EA200901331 A EA 200901331A EA 200901331 A EA200901331 A EA 200901331A EA 200901331 A1 EA200901331 A1 EA 200901331A1
Authority
EA
Eurasian Patent Office
Prior art keywords
targets
options
attention
identification
biological sample
Prior art date
Application number
EA200901331A
Other languages
English (en)
Inventor
Брюс Селигманн
Ральф Мартил
Мэтью Раунсэвил
Ихаб Бэутрэуз
Original Assignee
Хай Зрупут Дженомикс, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Хай Зрупут Дженомикс, Инк. filed Critical Хай Зрупут Дженомикс, Инк.
Publication of EA200901331A1 publication Critical patent/EA200901331A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение имеет отношение к композициям, аппаратам и методам, полезных для одновременно исполняемых единичных, множественных высокопроизводительных биологических или химических исследований с использованием молекул нуклеазной защиты, которые специфически связываются с интересующими мишенями. Молекулы нуклеазной протекции способны распознавать мишени в сложных биологических образцах, включая пресервированные, фиксированные, высушенные, и/или перекрестно-сшитые виды. Реагенты и методы настоящего исследования предоставляют эффективное средство для анализа интересующих мишеней из сложных биологических образцов без солюбилизации или разрушения образца. Использование таких методов в клиническом и/или диагностическом применении также описано.
EA200901331A 2007-03-30 2008-03-31 Способ определения по крайней мере одной нерастворимой мишени в биологическом образце (варианты) EA200901331A1 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US92081407P 2007-03-30 2007-03-30
US1871708P 2008-01-03 2008-01-03
US12/058,205 US20080268451A1 (en) 2007-03-30 2008-03-28 Measurement of an insoluble analyte in a sample
PCT/US2008/058837 WO2008121927A1 (en) 2007-03-30 2008-03-31 Measurement of an insoluble analyte in a sample

Publications (1)

Publication Number Publication Date
EA200901331A1 true EA200901331A1 (ru) 2010-04-30

Family

ID=39473893

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200901331A EA200901331A1 (ru) 2007-03-30 2008-03-31 Способ определения по крайней мере одной нерастворимой мишени в биологическом образце (варианты)

Country Status (8)

Country Link
US (1) US20080268451A1 (ru)
EP (1) EP2132332B1 (ru)
JP (1) JP5735798B2 (ru)
CN (1) CN101809165A (ru)
AU (1) AU2008232523B2 (ru)
CA (1) CA2682196A1 (ru)
EA (1) EA200901331A1 (ru)
WO (1) WO2008121927A1 (ru)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2778249C (en) * 2009-11-03 2018-12-04 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection sequencing (qnps)
WO2011146683A1 (en) * 2010-05-19 2011-11-24 Metabolon, Inc. Methods and reagents for metabolomics and histology in a biological sample and a kit for the same
ES2597032T3 (es) 2011-05-04 2017-01-13 HTG Molecular Diagnostics, Inc Mejoras en un ensayo cuantitativo de protección contra la nucleasa (qNPA) y secuenciación (qNPS)
CA3113672A1 (en) 2011-07-01 2013-01-10 Htg Molecular Diagnostics, Inc. Methods of detecting gene fusions
AU2012316129B2 (en) 2011-09-28 2017-10-05 Htg Molecular Diagnostics, Inc. Methods of co-detecting mRNA and small non-coding RNA
US9758829B2 (en) 2012-06-22 2017-09-12 Htg Molecular Diagnostics, Inc. Molecular malignancy in melanocytic lesions
WO2014005038A1 (en) * 2012-06-29 2014-01-03 Htg Molecular Diagnostics, Inc. Nuclease protection methods for detection of nucleotide variants
WO2017059108A1 (en) 2015-09-29 2017-04-06 Htg Molecular Diagnostics, Inc. Methods for subtyping diffuse b-cell lymphoma (dlbcl)
BR112018016240A2 (pt) 2016-02-11 2019-01-02 Htg Molecular Diagnostics Inc método de sequência alvo direta que utiliza proteção da nuclease
WO2018061877A1 (ja) * 2016-09-30 2018-04-05 栄研化学株式会社 核酸を抽出する方法及びそれに用いるキット
CN106706614B (zh) * 2016-11-21 2019-03-22 江苏华鸣生物科技有限公司 检测尿液中对羟基苯丙氨酸的试剂盒及其制备方法
CN110520541B (zh) 2017-02-13 2024-03-26 耶鲁大学 高通量单细胞多组学
WO2020236939A2 (en) 2019-05-23 2020-11-26 Paradigm Diagnostics Tissue preparation using nuclease
CN110779970B (zh) * 2019-09-18 2022-04-12 南京农业大学 一种鸡传染性支气管炎病毒h120株的电化学检测方法
WO2021142233A1 (en) * 2020-01-10 2021-07-15 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
EP4314818A4 (en) * 2021-04-01 2024-10-02 Siemens Healthcare Diagnostics, Inc. DIAGNOSTIC INSTRUMENTS WITH SORTING CAPABILITY AND SORTING METHODS THEREFOR
WO2023049835A1 (en) * 2021-09-23 2023-03-30 Georgia Tech Research Corporation Multiplexed electronic immunoassay using enzymatically amplified metallization on nanostructured surfaces

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2285445A (en) * 1993-12-06 1995-07-12 Pna Diagnostics As Protecting nucleic acids and methods of analysis
JP2001509662A (ja) 1996-04-19 2001-07-24 ファルマシア・アンド・アップジョン・カンパニー 疾患特異的遺伝子の定量および発見のための高容量ｉｎ―ｓｉｔｕ ｍＲＮＡ ハイブリダイゼーション法
US6900013B1 (en) * 2000-08-25 2005-05-31 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization

Also Published As

Publication number Publication date
CA2682196A1 (en) 2008-10-09
EP2132332B1 (en) 2014-01-22
EP2132332A1 (en) 2009-12-16
US20080268451A1 (en) 2008-10-30
CN101809165A (zh) 2010-08-18
AU2008232523B2 (en) 2013-05-16
JP2010523943A (ja) 2010-07-15
WO2008121927A1 (en) 2008-10-09
JP5735798B2 (ja) 2015-06-17
AU2008232523A1 (en) 2008-10-09

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