ECSP21033637A - Methods and Compositions for Editing RNAs - Google Patents
Methods and Compositions for Editing RNAsInfo
- Publication number
- ECSP21033637A ECSP21033637A ECSENADI202133637A ECDI202133637A ECSP21033637A EC SP21033637 A ECSP21033637 A EC SP21033637A EC SENADI202133637 A ECSENADI202133637 A EC SENADI202133637A EC DI202133637 A ECDI202133637 A EC DI202133637A EC SP21033637 A ECSP21033637 A EC SP21033637A
- Authority
- EC
- Ecuador
- Prior art keywords
- rna
- methods
- compositions
- editing
- present application
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 title 1
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 abstract 2
- 230000007115 recruitment Effects 0.000 abstract 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 abstract 1
- 238000010357 RNA editing Methods 0.000 abstract 1
- 230000026279 RNA modification Effects 0.000 abstract 1
- 229960005305 adenosine Drugs 0.000 abstract 1
- 230000009615 deamination Effects 0.000 abstract 1
- 238000006481 deamination reaction Methods 0.000 abstract 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- A—HUMAN NECESSITIES
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7125—Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- Health & Medical Sciences (AREA)
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Abstract
La presente solicitud proporciona métodos para editar ARN mediante la introducción de un ARN de reclutamiento de desaminasas en una célula hospedera para la desaminación de una adenosina en un ARN objetivo; la presente solicitud proporciona además ARN de reclutamiento de desaminasas usados en los métodos de edición de ARN y composiciones que los comprenden.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018110105 | 2018-10-12 | ||
| CN2019082713 | 2019-04-15 | ||
| PCT/CN2019/110782 WO2020074001A1 (en) | 2018-10-12 | 2019-10-12 | Methods and Compositions for Editing RNAs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP21033637A true ECSP21033637A (es) | 2021-08-31 |
Family
ID=70163607
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ECSENADI202133637A ECSP21033637A (es) | 2018-10-12 | 2021-05-12 | Methods and Compositions for Editing RNAs |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US20210355494A1 (es) |
| EP (3) | EP4527931A1 (es) |
| JP (2) | JP7252328B2 (es) |
| KR (1) | KR102666695B1 (es) |
| CN (4) | CN116218836A (es) |
| AU (2) | AU2019357450B2 (es) |
| BR (1) | BR112021006844A8 (es) |
| CA (1) | CA3115864A1 (es) |
| CL (1) | CL2021000880A1 (es) |
| CO (1) | CO2021005956A2 (es) |
| CR (1) | CR20210243A (es) |
| DK (1) | DK3864152T5 (es) |
| EC (1) | ECSP21033637A (es) |
| ES (1) | ES2962434T3 (es) |
| IL (1) | IL282191A (es) |
| MX (1) | MX2021004187A (es) |
| PE (1) | PE20211402A1 (es) |
| PH (1) | PH12021550805A1 (es) |
| SG (1) | SG11202103666PA (es) |
| TW (1) | TW202028466A (es) |
| WO (1) | WO2020074001A1 (es) |
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| CN110612353A (zh) | 2017-03-03 | 2019-12-24 | 加利福尼亚大学董事会 | 经由抑制性tRNAs和脱氨酶对突变进行RNA靶向 |
| PE20211402A1 (es) * | 2018-10-12 | 2021-08-02 | Univ Beijing | Metodos y composiciones para editar acidos ribonucleicos |
| CN113573717A (zh) | 2019-01-22 | 2021-10-29 | 科罗生物公司 | Rna编辑的寡核苷酸及其用途 |
| CA3127241A1 (en) | 2019-01-22 | 2020-07-30 | Korro Bio, Inc. | Rna-editing oligonucleotides and uses thereof |
| AU2020210900B2 (en) | 2019-01-22 | 2025-04-24 | Korro Bio, Inc. | RNA-editing oligonucleotides and uses thereof |
| CA3136735A1 (en) | 2019-04-15 | 2020-10-22 | Edigene Inc. | Methods and compositions for editing rnas |
| AU2020313143B2 (en) | 2019-07-12 | 2024-04-04 | Peking University | Targeted RNA editing by leveraging endogenous adar using engineered RNAs |
| US11827880B2 (en) | 2019-12-02 | 2023-11-28 | Shape Therapeutics Inc. | Therapeutic editing |
| AU2020418228A1 (en) | 2019-12-30 | 2022-08-18 | Edigene Therapeutics (Beijing) Inc. | Leaper technology based method for treating MPS IH and composition |
| AU2020418026A1 (en) | 2019-12-30 | 2022-08-18 | Edigene Therapeutics (Beijing) Inc. | Method for treating usher syndrome and composition thereof |
| PE20230703A1 (es) * | 2020-04-15 | 2023-04-24 | Edigene Therapeutics Beijing Inc | Metodo y farmaco para tratar el sindrome de hurler |
| CA3173012A1 (en) * | 2020-04-22 | 2021-10-28 | Susan BYRNE | Compositions and methods using snrna components |
| WO2021243023A1 (en) * | 2020-05-28 | 2021-12-02 | Korro Bio, Inc. | Methods and compositions for the adar-mediated editing of serpina1 |
| CN113897359A (zh) * | 2020-07-06 | 2022-01-07 | 博雅辑因(北京)生物科技有限公司 | 一种改善的rna编辑方法 |
| KR20230035362A (ko) * | 2020-07-06 | 2023-03-13 | 에디진 테라퓨틱스 (베이징) 인크. | 개선된 rna 편집 방법 |
| IL278401A (en) | 2020-10-29 | 2022-05-01 | Yeda Res & Dev | Polynucleotides for editing RNA and a method for using them |
| EP4240338A4 (en) * | 2021-01-04 | 2025-08-06 | Univ California | IN VIVO PROGRAMMABLE RNA EDITING BY RECRUITMENT OF ENDOGENOUS ADARS |
| JP2024504608A (ja) * | 2021-01-12 | 2024-02-01 | 北京大学 | 遺伝子操作rnaを使用した内因性adarの活用による標的化rnaの編集 |
| KR20240021893A (ko) * | 2021-06-15 | 2024-02-19 | 메사추세츠 인스티튜트 오브 테크놀로지 | 데아미나제 기반 rna 센서 |
| US20240279656A1 (en) * | 2021-06-29 | 2024-08-22 | Shape Therapeutics Inc. | Engineered Guide RNAs and Polynucleotides |
| TW202321451A (zh) * | 2021-08-18 | 2023-06-01 | 北京大學 | 工程化的 adar 招募 rnas 及其使用方法 |
| US20250102522A1 (en) | 2021-10-07 | 2025-03-27 | Hepatx Corporation | Methods of tracking donor cells in a recipient |
| EP4423266A1 (en) * | 2021-10-27 | 2024-09-04 | Shape Therapeutics Inc. | Engineered rnas |
| JP2024541983A (ja) * | 2021-10-29 | 2024-11-13 | デューク ユニバーシティ | Rnaプログラム可能な細胞編集のための組成物およびシステム、ならびにそれを作製および使用する方法 |
| WO2023143539A1 (en) * | 2022-01-28 | 2023-08-03 | Edigene Therapeutics (Beijing) Inc. | Engineered adar-recruiting rnas and methods of use thereof |
| WO2023185231A1 (en) * | 2022-04-02 | 2023-10-05 | Edigene Therapeutics (Beijing) Inc. | Engineered adar-recruiting rnas and methods of use for usher syndrome |
| WO2024228735A2 (en) * | 2022-07-15 | 2024-11-07 | Georgia Tech Research Corporation | Anti-sars-cov-1 and anti-sars-cov-2 activatable rnase guide sequences |
| EP4605529A1 (en) * | 2022-10-18 | 2025-08-27 | Regeneron Pharmaceuticals, Inc. | Compositions and methods comprising programmable snrnas for rna editing |
| US12565665B2 (en) | 2022-12-21 | 2026-03-03 | Trustees Of Boston University | Compositions and methods for controlled mRNA translation and stability |
| CN118620958A (zh) * | 2023-03-08 | 2024-09-10 | 广州医科大学 | 一种抗原表位的制备方法 |
| WO2024197951A1 (zh) * | 2023-03-31 | 2024-10-03 | 时夕(广州)生物科技有限公司 | 一种与rna编辑靶位点形成编辑底物的序列 |
| CN118853661A (zh) * | 2023-04-28 | 2024-10-29 | 时夕(广州)生物科技有限公司 | 编辑rna的方法、组合物及其应用 |
| WO2024235283A1 (en) * | 2023-05-16 | 2024-11-21 | Peking University | Method of modulating rna splicing |
| EP4763979A1 (en) | 2023-08-18 | 2026-06-24 | EdiGene Biotechnology, Inc. | Engineered t cell having prolonged in vivo half-life and preparation method therefor |
| WO2025206105A1 (ja) * | 2024-03-29 | 2025-10-02 | 株式会社Frest | 標的アデノシンに対する部位特異的編集を誘導するオリゴヌクレオチド |
| CN121059628A (zh) * | 2024-06-03 | 2025-12-05 | 时夕(广州)生物科技有限公司 | 调控RNA剪接的向导agRNA |
| CN119505015B (zh) * | 2024-11-15 | 2025-10-17 | 北京大学 | 一种融合蛋白及其应用方法 |
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|---|---|---|---|---|
| KR101297830B1 (ko) * | 2010-09-10 | 2013-08-19 | 한국생명공학연구원 | 신규한 rna 결합 단백질 및 이를 이용한 무표지 마이크로 rna 검출방법 |
| CN107532162A (zh) | 2014-12-12 | 2018-01-02 | 托德·M·伍尔夫 | 用于利用寡核苷酸编辑细胞中核酸的组合物和方法 |
| AU2015367378A1 (en) * | 2014-12-17 | 2017-06-15 | Proqr Therapeutics Ii B.V. | Targeted RNA editing |
| WO2017010556A1 (ja) | 2015-07-14 | 2017-01-19 | 学校法人福岡大学 | 部位特異的rna変異導入方法およびそれに使用する標的編集ガイドrnaならびに標的rna-標的編集ガイドrna複合体 |
| US11396672B2 (en) * | 2015-08-31 | 2022-07-26 | The University Of Chicago | Composition and methods for detecting adenosine modifications |
| DE102015012522B3 (de) * | 2015-09-26 | 2016-06-02 | Eberhard Karls Universität Tübingen | Verfahren und Substanzen zur gerichteten RNA-Editierung |
| EP3475424A1 (en) * | 2016-06-22 | 2019-05-01 | ProQR Therapeutics II B.V. | Single-stranded rna-editing oligonucleotides |
| WO2018041973A1 (en) * | 2016-09-01 | 2018-03-08 | Proqr Therapeutics Ii B.V. | Chemically modified single-stranded rna-editing oligonucleotides |
| US11274300B2 (en) | 2017-01-19 | 2022-03-15 | Proqr Therapeutics Ii B.V. | Oligonucleotide complexes for use in RNA editing |
| CN110612353A (zh) * | 2017-03-03 | 2019-12-24 | 加利福尼亚大学董事会 | 经由抑制性tRNAs和脱氨酶对突变进行RNA靶向 |
| CN110959039B (zh) | 2017-03-15 | 2025-03-14 | 博德研究所 | 新型cas13b直向同源物crispr酶和系统 |
| KR102338449B1 (ko) * | 2017-09-21 | 2021-12-10 | 더 브로드 인스티튜트, 인코퍼레이티드 | 표적화된 핵산 편집을 위한 시스템, 방법, 및 조성물 |
| PE20211402A1 (es) * | 2018-10-12 | 2021-08-02 | Univ Beijing | Metodos y composiciones para editar acidos ribonucleicos |
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