EP0109416B1 - Verfahren und vorrichtung zum verteilen, pipettieren und verdünnen von flüssigkeiten - Google Patents

Verfahren und vorrichtung zum verteilen, pipettieren und verdünnen von flüssigkeiten Download PDF

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Publication number
EP0109416B1
EP0109416B1 EP19830901609 EP83901609A EP0109416B1 EP 0109416 B1 EP0109416 B1 EP 0109416B1 EP 19830901609 EP19830901609 EP 19830901609 EP 83901609 A EP83901609 A EP 83901609A EP 0109416 B1 EP0109416 B1 EP 0109416B1
Authority
EP
European Patent Office
Prior art keywords
pump
tube
dispensing
fluid
pipetting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP19830901609
Other languages
English (en)
French (fr)
Other versions
EP0109416A4 (de
EP0109416A1 (de
Inventor
Gizella Balas
János CSIKOS
Gábor KEMENY
Zsolt Szabo
Ferenc Vadasz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
"labor" Mueszeripari Muevek
Original Assignee
"labor" Mueszeripari Muevek
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Publication date
Application filed by "labor" Mueszeripari Muevek filed Critical "labor" Mueszeripari Muevek
Publication of EP0109416A1 publication Critical patent/EP0109416A1/de
Publication of EP0109416A4 publication Critical patent/EP0109416A4/de
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Publication of EP0109416B1 publication Critical patent/EP0109416B1/de
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the invention relates to a method for dispensing, pipetting and consecutively diluting fluids, primarily for analytical laboratory use, wherein a constant stream of the fluid is established and maintained in a part of a tube by means of a pump and a predetermined amount of the fluid is dispensed by opening valve means in said tube on the pressure side of the pump. Furthermore, the invention relates to an apparatus for dispensing and pipetting fluids comprising a pump and controlled valve means in a tube on the pressure side of the pump.
  • the rare peristaltic pump type dispensers require a separately operated pump head for each dispensing channel.
  • precision syringe and displacement mechanism is needed for each additional channel.
  • the cost requirement in this latter case can be multiple compared to the price of the multichannel peristaltic pumps.
  • one of the main difficulties is in the lack of independent control of the channels.
  • the main object of this invention is to eliminate the deficiencies of the known solutions and to provide a method and an apparatus with which a fluid can be dispensed, pipetted and diluted in a more convenient and precise way.
  • this object is solved in such a way that in the method as described in the first paragraph hereinabove, a multichannel peristaltic pump is used, a predetermined amount of the fluid is sipped by opening valve means in the tube on the suction side of the pump for an adequate time, a predetermined amount of the fluid is dispensed by opening said valve means in the tube on the pressure side of the pump, and the control of both valve means is influenced by rotational speed fluctuations of the pump drive and/or by pulsation of the pump.
  • the fluid delivered by the pump is lead through a controlled two-way switch valve either into a fluid container on the suction side of the pump or, for dispensing, to a dispensing tube.
  • a controlled two-way switch valve either into a fluid container on the suction side of the pump or, for dispensing, to a dispensing tube.
  • the sucking effect of the pump in the dispensing tube may be controlled by a two-way switch valve.
  • the pump in the apparatus for dispensing and pipetting fluids comprising a pump and controlled valve means in a tube on the pressure side of the pump, the pump is a multichannel peristaltic pump, a pressure side tube of the pump is connected through a controlled switch valve to a fluid container on the suction side of the pump or to a dispensing tube, and a control unit is attached to said switch valve and is electrically connected to a sensor of a chopper fixed to an axis of the pump.
  • a suction side tube of the pump is connected through an additional controlled switch valve either to said fluid container or to a dispensing and pipetting tube.
  • a further pressure side tube may be connected to the fluid container and a further suction side tube may be connected through the additional controlled switch valve either to the fluid container or to the pipetting and dispensing tube.
  • the method and the apparatus according to this invention have the advantage that the fluid can be dispensed, pipetted and diluted in a most convenient and precise way.
  • the costs for manufacturing that apparatus and for. proceeding in accordance with that method are relatively low.
  • the apparatus shown in Fig. 1 has a peristaltic pump 1, a fluid container 2, a controlled valve 3a, tubes 4b, 5a, 5b, 6 and a control unit 7. With this system, fluid dispensing can be carried out according to the method of this invention.
  • FIG. 2 An apparatus capable to carry out pipetting is shown in Fig. 2, where an additional controlled switch-valve 3b is built into the sipping side of pump 1, i.e. into tube 4a.
  • Tubes 4d and 5d are connected to another channel of the peristaltic pump 1.
  • the peristaltic pump 1 delivers the contents of container 2 through tubes 4b, 5a towards controlled valve 3a, which is closed towards tube'6 and open towards tube 5b before the dispensing cycle. Thus, a constant rate of recirculation is maintained. If the valve 3a is switched over for a certain period of time determined by the control unit 7 the selected amount of liquid leaves the dispensing tube 6.
  • the timing signal for the control unit 7 is given by a chopper fixed to the axis of the peristaltic pump 1.
  • the frequency of the impulses is preferably unit multiple of the pulsation frequency of the pump, which is known to emanate from the rollers that lift off from the tubes one after the other.
  • the exact time of the valve switching has to be selected in such a manner that it occurs in all cases at the minimum point of the pulsation.
  • the minimum dispensable volume for each tube diameter of the pump is given by the distance of the rollers that is the "length" of the pressure pulses.
  • the frequency of the signal chopper and, thus, the minimum dispensable volume is limited only by the general technical parameters.
  • the channels of a multichannel peristaltic pump can independently be equipped with controlled valves as is shown in the figure.
  • each channel can be used for dispensing independently from each others without disturbing the even rotation of the pump.
  • a switch-valve 3b connected into the suction side of the pump 1.
  • Switch-valve 3b connects the suction side tube 4a either through tube 4b to the fluid container 2 or, in the other setting of the valve 3b, through tube 6b to the pipet and outlet tube 6c.
  • the timing signal arrives from the pump 1 and serves as a basis for the control of both valves.
  • the pipetting cycle of the system filled already with the fluid is as follows:
  • the pump 1 is circulating the fluid through tubes 4a, 4b, 5a, 5b.
  • the pipet tip 6c which contains some microliter air gap (see last step of the cycle) is immersed into the fluid sample to be pipetted.
  • the control unit 7 switches over the valve 3b for a short time, or for an appropriate number of pulses and, thus, the desired amount of sample will flow into tube 6c.
  • the sipping is finished and the pump works through tubes 4b and 5b again.
  • the pipet tip 6c is placed above the appropriate vessel and dispensing can be started. This occurs by switching over the valve 3a in the same manner as described with Fig. 1.
  • the active time of the valves 3a and 3b is equal.
  • the pipet tip 6c can be flushed by a small amount of fluid from inside.
  • the flush fluid if required serves for the dilution of the sample.
  • a little amount of air can be sucked by switching over valve 3b for a short time, while the tip is held in the air.
  • the total pipetting cycle time may be too long when using the arrangement in Fig. 2.
  • the embodiment of Fig. 3, built up in a different manner, while functionally is equivalent to that of Fig. 2.
  • the rate of outflow is determined by the cross section of the pump tube 4a, 5a, whereas the inflow sipping rate is determined by the delivery rate of the pump tube 4d, 5d.
  • the method according to the present invention makes it possible to utilize a multichannel peristaltic pump and more controlled valves to build different systems and establish further additional operational functions.
  • automatic titration will be possible with the system on Fig. 1 if an end point indicator is connected to the control unit.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Reciprocating Pumps (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (6)

1. Verfahren für die Ausgabe, Pipettierung und fortlaufende Verdünnung von Flüssigkeiten, bei dem ein konstanter Strom der Flüssigkeit mit Hilfe einer Pumpe aufgebaut und in einem Teil einer Röhre erhalten wird und bei dem eine festgelegte Menge der Flüssigkeit durch das Öffnen einer Ventilvorrichtung in der Röhre auf der Druckseite der Pumpe ausgegeben wird,
gekennzeichnet durch
den Einsatz einer Mehrweg-Peristaltik-Pumpe;
das Ansaugen einer vorher festgelegten Menge der Flüssigkeit durch das Öffnen der Ventilvorrichtung in der Röhre auf der Saugseite der Pumpe für eine angepaßte Zeitspanne;
die Ausgabe einer festgelegten Menge der Flüssigkeit durch Öffnen der Ventilvorrichtung in der Röhre auf der Druckseite der Pumpe;
die Beeinflussung der Steuerung der beiden Ventilvorrichtungen durch Schwankungen der Rotationsgeschwindigkeit des Pumpenantriebs und/oder durch Pulsieren der Pumpe.
2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß die Flüssigkeit, die durch die Pumpe gefördert wird, durch ein gesteuertes Zwei-Wege-Ventil entweder in einen Flüssigkeitsbehälter auf der Saugseite der Pumpe oder zur Ausgabe in eine Ausgaberöhre geleitet wird.
3,Verfahren nach Anspruch 2, dadurch gekennzeichnet, daß der Saugeffekt der Pumpe in der Ausgaberöhre durch ein Zwei-Wege-Ventil gesteuert wird.
4. Vorrichtung für die Ausgabe und Pipettierung von Flüssigkeiten, die eine Pumpe und eine gesteuerte Ventilvorrichtung in einer Röhre auf der Druckseite der Pumpe aufweist, dadurch gekennzeichnet, daß die Pumpe eine Mehrweg-Peristaltik-Pumpe (1) ist, eine Druckseitenröhre (5a) der Pumpe (1) über ein gesteuertes Schaltventil (3a) mit einem Flüssigkeitsbehälter (2) auf der Saugseite der Pumpe (1) oder mit einer Ausgaberöhre (6) verbunden ist und eine Steuereinheit (7) an dem Schaltventil (3a) angebracht und elektrisch mit einem Sensor eines Zerhackers verbunden ist, der an der Welle der Pumpe (1) befestigt ist.
5. Vorrichtung nach Anspruch 4, dadurch gekennzeichnet, daß eine Saugseitenröhre (4a) der Pumpe (1) über ein zusätzliches gesteuertes Schaltventil (3b) entweder mit dem Flüssigkeitsbehälter (2) oder mit einer Ausgabe- und Pipettierröhre (6c) verbunden ist.
6. Vorrichtung nach Anspruch 5, dadurch gekennzeichnet, daß eine weitere Druckröhre (5d) mit dem Flüssigkeitsbehälter (2) verbunden ist und eine weitere Saugseitenröhre (4d) über ein zusätzliches gesteuertes Schaltventil (3b) entweder mit dem Flüssigkeitsbehälter (2) oder mit der Pipettier- und Ausgaberöhre (6c) verbunden ist.
EP19830901609 1982-05-19 1983-05-19 Verfahren und vorrichtung zum verteilen, pipettieren und verdünnen von flüssigkeiten Expired EP0109416B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU159082 1982-05-19
HU159082A HU183961B (en) 1982-05-19 1982-05-19 Method and apparatus for measuring, pipetting and diluting following that fluid quantities

Publications (3)

Publication Number Publication Date
EP0109416A1 EP0109416A1 (de) 1984-05-30
EP0109416A4 EP0109416A4 (de) 1984-09-14
EP0109416B1 true EP0109416B1 (de) 1988-05-18

Family

ID=10955183

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19830901609 Expired EP0109416B1 (de) 1982-05-19 1983-05-19 Verfahren und vorrichtung zum verteilen, pipettieren und verdünnen von flüssigkeiten

Country Status (5)

Country Link
EP (1) EP0109416B1 (de)
DE (1) DE3376680D1 (de)
HU (1) HU183961B (de)
SU (1) SU1276257A3 (de)
WO (1) WO1983004015A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU659153B2 (en) * 1991-05-20 1995-05-11 Langoulant, Bruce Le Ber Beverage dispensing apparatus
DE19616868A1 (de) * 1996-04-26 1997-11-06 Hubert Goederle Vorrichtung zur automatisierten Ausgabe von fließfähigen Medien, insbesondere von Getränken
DE19616869A1 (de) * 1996-04-26 1997-11-06 Hubert Goederle Vorrichtung zur automatisierten Ausgabe von fließfähigen Medien, insbesondere von Getränken
ATE552507T1 (de) 2009-04-30 2012-04-15 Hoffmann La Roche System und verfahren zum pipettieren von fluiden, verfahren zum kalibrieren des systems
WO2022044827A1 (ja) * 2020-08-27 2022-03-03 ソニーグループ株式会社 液体吐出装置、液体吐出制御方法及び充填方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3419358A (en) * 1965-10-19 1968-12-31 Technicon Corp Automatic analysis apparatus and method
US3900289A (en) * 1971-04-12 1975-08-19 Abbott Lab Apparatus and method for filling a compartment
US3982667A (en) * 1975-11-24 1976-09-28 Hyperion Incorporated Diluting liquid samples

Also Published As

Publication number Publication date
EP0109416A4 (de) 1984-09-14
EP0109416A1 (de) 1984-05-30
HU183961B (en) 1984-06-28
SU1276257A3 (ru) 1986-12-07
WO1983004015A1 (en) 1983-11-24
DE3376680D1 (en) 1988-06-23

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