EP0281958A2 - Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration - Google Patents

Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration Download PDF

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Publication number
EP0281958A2
EP0281958A2 EP88103357A EP88103357A EP0281958A2 EP 0281958 A2 EP0281958 A2 EP 0281958A2 EP 88103357 A EP88103357 A EP 88103357A EP 88103357 A EP88103357 A EP 88103357A EP 0281958 A2 EP0281958 A2 EP 0281958A2
Authority
EP
European Patent Office
Prior art keywords
vessels
mixing
contents
microtitration plate
cavity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP88103357A
Other languages
German (de)
English (en)
Other versions
EP0281958A3 (fr
Inventor
Jürgen Dr. Gross
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of EP0281958A2 publication Critical patent/EP0281958A2/fr
Publication of EP0281958A3 publication Critical patent/EP0281958A3/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50851—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
    • B01F33/407—Mixers using gas or liquid agitation, e.g. with air supply tubes by blowing gas on the material from above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50853—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates with covers or lids

Definitions

  • the invention relates to a device for thermostatting and mixing the contents of vessels of a microtitration plate for immunological tests.
  • antigens are surface-fixed on the vessel walls of a microtitration plate with which the antibodies (antigens) present in the sample bind during the first incubation period. After washing out unbound sample components, enzyme conjugate is dosed. After the second incubation has ended, excess conjugate is removed by washing and substrate is dosed. After the third incubation, the implementation is stopped. All steps are carried out at a certain temperature. The vessels must therefore be thermostatted. A disadvantage is not only the complex thermostatting of the microtitration plate, but also the relatively long incubation time. The invention seeks to remedy this.
  • a device which is characterized in that a hood corresponding to the surface of the microtitration plate is designed as a cavity which is closed on all sides, into which a gas line opens and whose boundary wall facing the microtitration plate is provided with gas outlet openings which are eccentric with respect to the Vessel axes are arranged and directed at an angle ⁇ to the surfaces of the liquids in the vessels.
  • the device offered the advantage that the liquid in the vessels was replaced by thermostated gas a circular movement is set, which not only improves the thermostatting of the liquid, but also the binding and reaction of the reagents with the samples in the wall area. This results in shorter incubation times and thus shorter test times.
  • the device consists essentially of a hood 1, the dimension of which corresponds to the area of the microtitration plate 2 with the vessels 7 and which is provided with a cavity 3 into which a gas line 4 for thermostatted gas, for. B. air from 30 ° to 50 ° C opens.
  • the boundary wall 5 for the cavity 3 of the hood 1 facing the microtitration plate 2 is provided with gas outlet openings 6.
  • the cavity 3 serves as a gas distributor for the gas outlet openings 6.
  • the gas outlet openings 6 can be designed as nozzles or cannulas. These are placed in the boundary wall 5 so that they are arranged eccentrically with respect to the vessel axes 8.
  • the air flow creates an indentation 11 on the radius R when it hits the liquid surface 10.
  • the nozzle axis is inclined by the angle ⁇ with respect to the surface 10.
  • the distance of the gas outlet opening from the vessel wall in the radial direction can be 0.2 to 0.5 vessel radii and the angle ⁇ at which the gas outlet opening is inclined toward the liquid surface can be 40 ° to 80 °.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Food-Manufacturing Devices (AREA)
EP88103357A 1987-03-07 1988-03-04 Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration Withdrawn EP0281958A3 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3707368 1987-03-07
DE19873707368 DE3707368A1 (de) 1987-03-07 1987-03-07 Vorrichtung zum thermostatisieren und mischen des inhaltes von gefaessen einer mikrotitrationsplatte

Publications (2)

Publication Number Publication Date
EP0281958A2 true EP0281958A2 (fr) 1988-09-14
EP0281958A3 EP0281958A3 (fr) 1989-03-22

Family

ID=6322515

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88103357A Withdrawn EP0281958A3 (fr) 1987-03-07 1988-03-04 Dispositif de régulation de température et de mélange du contenu d'une plaque de micro titration

Country Status (7)

Country Link
EP (1) EP0281958A3 (fr)
JP (1) JPS63242335A (fr)
AU (1) AU1264588A (fr)
DE (1) DE3707368A1 (fr)
DK (1) DK119288A (fr)
FI (1) FI880988A7 (fr)
NO (1) NO880974L (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0589363A1 (fr) * 1992-09-25 1994-03-30 Boehringer Mannheim Gmbh Procédé et dispositif de mélange automatique sans contact d'un mélange de réaction dans un dispositif d'analyse
WO1994019097A1 (fr) * 1993-02-26 1994-09-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procede et dispositif permettant de melanger des liquides dans des plaques de microtitrage
WO1999017881A1 (fr) * 1997-10-07 1999-04-15 The Perkin-Elmer Corporation Appareil pour cycleur thermique a impact de fluide
EP1174703A3 (fr) * 2000-07-18 2003-04-16 Volker Barkey Evaporateur pour échantillons
WO2004008154A1 (fr) * 2002-07-16 2004-01-22 H+P Labortechnik Ag Station de traitement d'echantillons
EP1362115A4 (fr) * 2001-02-08 2004-06-23 Irm Llc Fermenteur multi-echantillons et son mode d'utilisation
WO2008000312A1 (fr) * 2006-06-29 2008-01-03 Ika-Werke Gmbh & Co.Kg Plaque de microtitration comprenant des éléments d'agitation
ITPD20080371A1 (it) * 2008-12-17 2010-06-18 Univ Padova Dispositivo di miscelazione meccanica per piastre di coltura
US9156012B2 (en) 2004-06-11 2015-10-13 Roche Diagnostics Operations, Inc. Method and device for mixing samples on a support
DE102019103029A1 (de) * 2019-02-07 2020-08-13 Thyssenkrupp Ag Verfahren zur Handhabung von Schmelzproben in einem Stahlwerkslabor sowie ein Stahlwerkslabor

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0639266A (ja) * 1992-07-04 1994-02-15 Kyowa Medex Co Ltd 液体攪拌方法及びその装置
JP3567187B2 (ja) * 1995-02-23 2004-09-22 アークレイ株式会社 試料液等の撹拌装置
DE19712575C2 (de) * 1997-03-26 2000-05-11 Desaga Gmbh C Inkubationsvorrichtung
DE19815696C2 (de) * 1998-04-08 2000-06-29 Gsf Forschungszentrum Umwelt Temperaturgeregelte Überströmapparatur für biologische Proben
DE10036159B4 (de) * 2000-07-25 2006-11-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrotiterplatten mit Begasung
DE10144225C1 (de) * 2001-09-08 2003-02-06 Eppendorf Ag Verfahren zum Trocknen von Filtern, die in Kavitäten einer Mikrotiterfilterplatte angeordnet sind
DE10329983A1 (de) * 2003-06-27 2005-03-31 Siemens Ag Mikroreaktorsystem mit einer Reaktionsräume aufweisenden Trägerplatte und Verfahren zum Betrieb desselben
JP2006343245A (ja) * 2005-06-09 2006-12-21 Olympus Corp 分注装置および分析装置
DE102005034175A1 (de) * 2005-07-21 2007-01-25 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Erzeugung eines Feuchtluftstromes mit definierter relativer Feuchte
WO2007063929A1 (fr) * 2005-12-02 2007-06-07 Wako Pure Chemical Industries, Ltd. Appareil d’agitation de liquide et procede
JP2007318170A (ja) * 2007-08-06 2007-12-06 Sanyo Electric Co Ltd 固体電解コンデンサの製造方法及び製造装置
JP5659698B2 (ja) * 2010-10-29 2015-01-28 ソニー株式会社 試料流入装置、試料流入チップ及び試料流入方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3398935A (en) * 1964-03-25 1968-08-27 Bausch & Lomb Mixing means
DE3578454D1 (de) * 1984-02-01 1990-08-02 Baxter Int Klinisches analysesystem und verfahren.
DE3434931A1 (de) * 1984-09-22 1986-03-27 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Verfahren und vorrichtung zum mischen einer zu analysierenden fluessigkeitsprobe
DE3441179C2 (de) * 1984-11-10 1987-02-26 Dynatech Deutschland GmbH, 7306 Denkendorf Temperiereinrichtung für Mikro-Küvettenanordnungen, insbesondere für Mikrotitrationsplatten
FI852736A0 (fi) * 1985-07-10 1985-07-10 Labsystems Oy Termosterbar kyvettenhet.

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362147A (en) * 1992-09-25 1994-11-08 Boehringer Mannheim Gmbh Method and apparatus for the contactless automatic mixing of a reaction mixture in an analysis unit
EP0589363A1 (fr) * 1992-09-25 1994-03-30 Boehringer Mannheim Gmbh Procédé et dispositif de mélange automatique sans contact d'un mélange de réaction dans un dispositif d'analyse
WO1994019097A1 (fr) * 1993-02-26 1994-09-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procede et dispositif permettant de melanger des liquides dans des plaques de microtitrage
WO1999017881A1 (fr) * 1997-10-07 1999-04-15 The Perkin-Elmer Corporation Appareil pour cycleur thermique a impact de fluide
EP1174703A3 (fr) * 2000-07-18 2003-04-16 Volker Barkey Evaporateur pour échantillons
EP1362115A4 (fr) * 2001-02-08 2004-06-23 Irm Llc Fermenteur multi-echantillons et son mode d'utilisation
DE10232202B4 (de) * 2002-07-16 2005-08-25 H+P Labortechnik Ag Probenbehandlungsstation
DE10232202A1 (de) * 2002-07-16 2004-02-05 Herz, Helmut, Dr.-Ing. Probenbehandlungsstation
WO2004008154A1 (fr) * 2002-07-16 2004-01-22 H+P Labortechnik Ag Station de traitement d'echantillons
US9156012B2 (en) 2004-06-11 2015-10-13 Roche Diagnostics Operations, Inc. Method and device for mixing samples on a support
WO2008000312A1 (fr) * 2006-06-29 2008-01-03 Ika-Werke Gmbh & Co.Kg Plaque de microtitration comprenant des éléments d'agitation
US20100034048A1 (en) * 2006-06-29 2010-02-11 Ika-Werke Gmbh & Co. Kg Microtiter Plate with Stirring Elements
CN101484238B (zh) * 2006-06-29 2012-02-01 艾卡工厂有限及两合公司 具有搅拌元件的微量滴定板
US8317389B2 (en) 2006-06-29 2012-11-27 Ika-Werke Gmbh & Co. Kg Microtiter plate with stirring elements
TWI380852B (zh) * 2006-06-29 2013-01-01 Ika Werke Gmbh & Co Kg 具攪拌元件之微量滴定盤
ITPD20080371A1 (it) * 2008-12-17 2010-06-18 Univ Padova Dispositivo di miscelazione meccanica per piastre di coltura
DE102019103029A1 (de) * 2019-02-07 2020-08-13 Thyssenkrupp Ag Verfahren zur Handhabung von Schmelzproben in einem Stahlwerkslabor sowie ein Stahlwerkslabor
US12123862B2 (en) 2019-02-07 2024-10-22 thyssenkrupp Polysius GmbH Method for handling melt samples in a steelworks laboratory, and steelworks laboratory

Also Published As

Publication number Publication date
DE3707368A1 (de) 1988-09-15
AU1264588A (en) 1988-09-08
NO880974D0 (no) 1988-03-04
JPS63242335A (ja) 1988-10-07
DK119288A (da) 1988-09-08
FI880988L (fi) 1988-09-08
NO880974L (no) 1988-09-08
EP0281958A3 (fr) 1989-03-22
DK119288D0 (da) 1988-03-04
FI880988A0 (fi) 1988-03-03
FI880988A7 (fi) 1988-09-08

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