EP1510079A1 - Procede et systeme pour analyser un enregistrement optique - Google Patents

Procede et systeme pour analyser un enregistrement optique

Info

Publication number
EP1510079A1
EP1510079A1 EP03735532A EP03735532A EP1510079A1 EP 1510079 A1 EP1510079 A1 EP 1510079A1 EP 03735532 A EP03735532 A EP 03735532A EP 03735532 A EP03735532 A EP 03735532A EP 1510079 A1 EP1510079 A1 EP 1510079A1
Authority
EP
European Patent Office
Prior art keywords
elements
color
image sensor
information
colors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03735532A
Other languages
German (de)
English (en)
Inventor
Urs Schmid
Ruedi Rottermann
Daniel GÖGGEL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems Schweiz AG
Original Assignee
Leica Microsystems Schweiz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems Schweiz AG filed Critical Leica Microsystems Schweiz AG
Publication of EP1510079A1 publication Critical patent/EP1510079A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/80—Camera processing pipelines; Components thereof
    • H04N23/84—Camera processing pipelines; Components thereof for processing colour signals
    • H04N23/843—Demosaicing, e.g. interpolating colour pixel values
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/10—Circuitry of solid-state image sensors [SSIS]; Control thereof for transforming different wavelengths into image signals
    • H04N25/11—Arrangement of colour filter arrays [CFA]; Filter mosaics
    • H04N25/13—Arrangement of colour filter arrays [CFA]; Filter mosaics characterised by the spectral characteristics of the filter elements
    • H04N25/134—Arrangement of colour filter arrays [CFA]; Filter mosaics characterised by the spectral characteristics of the filter elements based on three different wavelength filter elements

Definitions

  • the invention relates to a method and a system for evaluating an optical image recorded by means of an image sensor.
  • the image sensor comprises a large number of light-sensitive elements, each of which is provided for determining a brightness value of exactly one color from a predetermined number of colors. With each element, information about the respectively assigned color is thus obtained.
  • the invention further relates to a use of the system mentioned as well as a computer program and a computer program product.
  • CCD Charge-Coupled Device
  • Elements are usually provided for the three primary colors red, green and blue, which are usually arranged in the so-called Bayer mosaic.
  • a striking feature of this is that there are two green filters for each red and one blue filter. Therefore capture typical Sensors 50% of the green, 25% of the blue and 25% of the red light.
  • a method for determining missing color values for pixels in a color filter array is known, for example, from US Pat. No. 6,181,376 B1. In the method, interpolation of known color values is carried out along diagonal lines.
  • Document EP 0 720 387 A2 describes a method and a device for generating interlaced images from a sensor with progressive scanning in an electronic camera. The clock rate required is reduced while the interlaced pixel values are provided for all colors in the same area.
  • the method according to the invention for evaluating an optical image recorded by means of an image sensor comprising a multiplicity of light-sensitive elements and each of the elements being provided for determining a brightness value of exactly one color from a predetermined number of colors, so that with information on the respectively assigned color is obtained for each element, characterized in that one of the colors is selected from the predetermined number of colors and only information of the elements which are provided for this color are taken into account in the evaluation.
  • the elements of the image sensor are preferably arranged in a matrix in rows and columns. In this case it is advisable that only even or odd lines of the image sensor are read out. In the field generated in this way, only the information of the elements that are provided for the selected color are then taken into account for the evaluation.
  • the elements are usually provided for the three primary colors red, green and blue, the elements preferably being arranged in a Bayer mosaic.
  • the elements are provided with color microfilters. These ensure that only light of a certain wavelength range can be transmitted and thus absorbed by the element in question, so that each element only provides information about the brightness or intensity of the light of a wavelength range and thus about a color, ie is intended for this.
  • the information of the elements provided for the selected color is usually displayed as an image on a display unit.
  • the method according to the invention allows rapid focusing, object positioning and magnification adjustment.
  • the system according to the invention for evaluating an optical image has an image sensor for capturing the optical image and a computing unit for processing information obtained by means of the image sensor.
  • the image sensor comprises a multiplicity of light-sensitive elements, each of which is provided for determining a brightness value of exactly one color from a predetermined number of colors.
  • the system is characterized in that the computing unit is designed to select one of the colors, preferably the one with the largest associated information content, and is also designed to take into account only information of the elements intended for the selected color in the evaluation.
  • the elements of the image sensor are preferably arranged in a matrix in rows and columns.
  • the computing unit is designed such that it reads out only even or odd lines of the image sensor. This selection is preferably also made by the computing unit.
  • the system is particularly suitable for evaluating monochromatic images, such as, for example, images in microscopy, in particular fluorescence microscopy.
  • monochromatic images such as, for example, images in microscopy, in particular fluorescence microscopy.
  • microscopy is often only a monochromatic image, such as when examining an object's fluorescence.
  • color information is recorded with four pixels. The color is then calculated for each pixel using an interpolation with the surrounding pixel colors. This is time consuming. In the case of a single-color image, the remaining pixels have no brightness information and therefore only provide noise, which can lead to image distortions. This was not taken into account in conventional readout and evaluation methods.
  • the frame rate when focusing monochromatic images can be practically doubled compared to a conventional method, since one of the two fields is not used at all.
  • the display of the color separation can also be accelerated compared to known methods.
  • the color pixels that do not belong to the selected color separation are omitted and therefore cannot interfere with the color separation due to noise information contained therein.
  • the result is a low-noise, fast grayscale image for optimal focusing and image detail determination.
  • the new method can be used to support a CCD image sensor or a digital camera by supporting special so-called readout modes.
  • the method is particularly recommended for high-resolution image sensors, since the reduction in resolution caused by the method is not important. There is even a reduction in live image displays the number of pixels required in order to be able to conveniently determine the desired image section (field of view).
  • the computer program according to the invention comprises program code means to carry out all steps of a method described above. It is executed on a computer or a corresponding computing unit.
  • the computer program product according to the invention is stored on a computer-readable data carrier.
  • Figure 1 illustrates the known field method.
  • FIG. 2 shows schematically an embodiment of the method according to the invention.
  • FIG. 3 schematically shows a further embodiment of the method according to the invention.
  • FIG. 4 schematically shows yet another embodiment of the method according to the invention.
  • FIG. 5 shows a possible application of the method according to the invention in a schematic representation.
  • FIG. 1 the conventional field method is shown schematically for explanation.
  • An image sensor 10 can be seen, which has a multiplicity of light-sensitive elements arranged in a matrix in rows and columns, elements 12 for the color green, elements 14 for the color red and elements 16 for the color blue being provided.
  • the elements 12 are thus provided with a green color microfilter, the elements 14 with a red color microfilter and the elements 16 with a blue color microfilter.
  • the elements 12, 14 and 16 for the three primary colors red, green and blue are arranged in the so-called Bayer mosaic. There are two green filters for each red and blue filter.
  • a first field 18 and for the second, fourth, sixth, eighth and tenth lines, i. H. for the even lines of the image sensor 10 a second field 20 is generated.
  • the two fields 18 and 20 are generated by first exposing the respective lines and then reading them out.
  • FIGS. An image sensor 30 can again be seen, which has a multiplicity of elements 32, 34 and 36 arranged in the form of a matrix, elements 32 being provided for the color green, elements 34 for the color red and elements 36 for the color blue.
  • a first field 38 is first generated, i. H. only the odd lines of the image sensor 30 are read out. This field 38 shows only green and red pixels. Subsequently, only the elements 34 for the color red are taken into account and the result is shown in an image 40 which shows a red color separation. The elements 32 or their information received are not taken into account in the further evaluation.
  • the number of pixels in the image 40 is a factor 4 less than the number of pixels in the sensor 30. However, since no interpolations have to be carried out, the image 40 can be determined quickly. With this low-noise image 40, a focusing or an image detail evaluation is then possible.
  • a second field 42 is correspondingly reproduced, which results from reading out the straight lines of the image sensor 30.
  • a further image 44 which reproduces a blue color separation, is determined from this field 42 by taking only the elements 36 into account for the color blue.
  • FIG. 4 shows the field 38 from FIG. 2 again.
  • an image 46 is generated from the field 38, which reproduces a green color separation.
  • the green color separation can be obtained from the first field 38 or the second field 42.
  • the display of the color separation can be accelerated by foregoing color interpolation compared to conventional methods.
  • the color pixels that do not belong to the selected color separation are omitted and therefore cannot interfere with the color separation due to noise information contained therein.
  • FIG. 5 shows a possible application for the method according to the invention in a schematic representation.
  • An object 50 to be recorded an objective 52, a dichroic beam splitter 54, a blocking filter 56, a tube lens 58, a microscope axis 60, an image plane or a sensor 62, an excitation filter 64, a collector lens 66, a light source 68 and one can be seen Illumination axis 70.
  • a fluorescent object 50 lies in the focal plane of the objective 52 and is imaged by the latter and the tube lens 58 onto the sensor 62.
  • a zone with a so-called parallel beam path is formed between the objective 52 and the tube lens 58. Additional elements can optionally be inserted in this zone. In fluorescence microscopy, this is usually the beam splitter 54 shown in FIG. 5 for coupling in an illumination and the blocking filter 56.
  • the blocking filter 56 only allows light with the wavelength of the fluorescent radiation emitted by the object 50 to pass through.
  • the short-wave light for fluorescence excitation is not let through by the blocking filter 56 and consequently cannot contribute to the image formation.
  • the light from the light source 68 is focused by the collector lens 66 and reflected by the beam splitter 54 in the direction of the object 50.
  • the excitation filter 64 only allows light of the wavelength that is used to excite the fluorescence to pass. Illumination light from the light source 68 of the longer-wave fluorescence radiation is blocked by the excitation filter 64 and thus cannot overlap the fluorescence radiation and impair its visibility.
  • the beam splitter 54 As a dichroic beam splitter 54, since this reflects the short-wave excitation light to a high degree and has a high transmission for the long-wave fluorescent radiation.

Landscapes

  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Color Television Image Signal Generators (AREA)

Abstract

L'invention concerne un procédé et un système pour analyser un enregistrement optique effectué par un capteur d'images (30), lequel comprend une pluralité d'éléments photosensibles (32, 34, 36), chacun d'eux servant à déterminer une luminosité d'une couleur précise parmi un nombre donné de couleurs, de sorte que chaque élément (32, 34, 36) permet d'obtenir des informations concernant la couleur qui lui correspond. L'invention est caractérisée en ce qu'une des couleurs est sélectionnée et que, lors de l'analyse, seules les informations des éléments (32, 34, 36) associés à ladite couleur sont prises en considération. La présente invention porte également sur une utilisation dudit système, sur un programme informatique et sur un produit logiciel qui permettent de réaliser ledit procédé.
EP03735532A 2002-06-06 2003-06-03 Procede et systeme pour analyser un enregistrement optique Withdrawn EP1510079A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10226274 2002-06-06
DE10226274A DE10226274B4 (de) 2002-06-06 2002-06-06 Verfahren und System zum Auswerten einer optischen Aufnahme
PCT/EP2003/005807 WO2003105485A1 (fr) 2002-06-06 2003-06-03 Procede et systeme pour analyser un enregistrement optique

Publications (1)

Publication Number Publication Date
EP1510079A1 true EP1510079A1 (fr) 2005-03-02

Family

ID=29719017

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03735532A Withdrawn EP1510079A1 (fr) 2002-06-06 2003-06-03 Procede et systeme pour analyser un enregistrement optique

Country Status (4)

Country Link
EP (1) EP1510079A1 (fr)
JP (1) JP2005529557A (fr)
DE (1) DE10226274B4 (fr)
WO (1) WO2003105485A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005013045B4 (de) 2005-03-18 2013-03-14 Siemens Aktiengesellschaft Fluoreszenz-Scanner für molekulare Signaturen
DE102005013044B4 (de) * 2005-03-18 2007-08-09 Siemens Ag Fluoreszenz-Scanner
DE102005013042A1 (de) 2005-03-18 2006-09-28 Siemens Ag Einrichtung zur Erzeugung von 3D-Fluoreszenz-oder Lumineszenz-Scans

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2000138942A (ja) * 1998-10-28 2000-05-16 Hyundai Electronics Ind Co Ltd イメ―ジセンサにおける緑色画素の平均値を利用する自動露出調節装置及びその方法
JP2000224599A (ja) * 1999-01-28 2000-08-11 Fuji Photo Film Co Ltd 固体撮像装置および信号読出し方法
US6850277B1 (en) * 1999-01-28 2005-02-01 Fuji Photo Film Co., Ltd. Solid-state image pickup apparatus with high-speed photometry and a signal reading method therefor

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JPH04284087A (ja) * 1991-03-13 1992-10-08 Canon Inc 電子スチルカメラ
JP2819365B2 (ja) * 1992-05-28 1998-10-30 キヤノン株式会社 画像形成装置
JPH08163380A (ja) * 1994-12-09 1996-06-21 Fujitsu Ltd 画像読取方法及び画像読取装置
JPH08242410A (ja) * 1994-12-30 1996-09-17 Eastman Kodak Co 電子カメラの順次走査センサからインタレースされた画像を作成する電子カメラ
US5668597A (en) * 1994-12-30 1997-09-16 Eastman Kodak Company Electronic camera with rapid automatic focus of an image upon a progressive scan image sensor
EP0774865A3 (fr) * 1995-11-17 2000-06-07 SANYO ELECTRIC Co., Ltd. Caméra vidéo avec mode à grande vitesse
US6181376B1 (en) * 1997-10-14 2001-01-30 Intel Corporation Method of determining missing color values for pixels in a color filter array
US6975354B2 (en) * 2000-06-29 2005-12-13 Texas Instruments Incorporated Digital still camera color filter array interpolation system and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000138942A (ja) * 1998-10-28 2000-05-16 Hyundai Electronics Ind Co Ltd イメ―ジセンサにおける緑色画素の平均値を利用する自動露出調節装置及びその方法
US6784939B1 (en) * 1998-10-28 2004-08-31 Hyundai Electronics Industries Co., Ltd. Apparatus and method for automatically controlling exposure time using a mean value of green pixels
JP2000224599A (ja) * 1999-01-28 2000-08-11 Fuji Photo Film Co Ltd 固体撮像装置および信号読出し方法
US6850277B1 (en) * 1999-01-28 2005-02-01 Fuji Photo Film Co., Ltd. Solid-state image pickup apparatus with high-speed photometry and a signal reading method therefor

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 08 6 October 2000 (2000-10-06) *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 11 3 January 2001 (2001-01-03) *
See also references of WO03105485A1 *

Also Published As

Publication number Publication date
WO2003105485A1 (fr) 2003-12-18
JP2005529557A (ja) 2005-09-29
DE10226274A1 (de) 2004-01-08
DE10226274B4 (de) 2006-07-06

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