EP2296795A1 - Système de mélange - Google Patents

Système de mélange

Info

Publication number
EP2296795A1
EP2296795A1 EP09753610A EP09753610A EP2296795A1 EP 2296795 A1 EP2296795 A1 EP 2296795A1 EP 09753610 A EP09753610 A EP 09753610A EP 09753610 A EP09753610 A EP 09753610A EP 2296795 A1 EP2296795 A1 EP 2296795A1
Authority
EP
European Patent Office
Prior art keywords
drive
mixing
mixer shaft
mixing system
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09753610A
Other languages
German (de)
English (en)
Other versions
EP2296795B1 (fr
Inventor
Oscar-Werner Reif
Jürgen VAN DEN BOOGAARD
Gerhard Greller
Marco Lohrengel
Karl-Heinz Kompart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Stedim Biotech GmbH
Original Assignee
Sartorius Stedim Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech GmbH filed Critical Sartorius Stedim Biotech GmbH
Publication of EP2296795A1 publication Critical patent/EP2296795A1/fr
Application granted granted Critical
Publication of EP2296795B1 publication Critical patent/EP2296795B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/441—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/10—Maintenance of mixers
    • B01F35/145—Washing or cleaning mixers not provided for in other groups in this subclass; Inhibiting build-up of material on machine parts using other means
    • B01F35/146—Working under sterile conditions; Sterilizing the mixer or parts thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/51—Mixing receptacles characterised by their material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/513—Flexible receptacles, e.g. bags supported by rigid containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/60—Safety arrangements
    • B01F35/605—Safety devices concerning the operation of the mixer

Definitions

  • the invention relates to a mixing system with a disposable container with flexible walls and a mixing device for single use, in particular for mixing sterile media or cell cultures.
  • Plastic disposable containers are increasingly being used in the biotechnology and pharmaceutical industries to mix liquids, dissolve solids in liquids, or to augment cells and microorganisms.
  • Plastic disposable containers have the advantage that they can already be supplied cleanly and sterile by the supplier, eliminating the need for elaborate cleaning procedures and validations prior to commissioning.
  • rotating mixer shafts which are provided with mixing elements such as propellers or vibratory mixer, in which a perforated mixer plate is placed in a linear up and down movement.
  • One of the biggest problems in this context is to couple a generally non-sterile drive to the sterile mixing shaft in the container interior while maintaining sterility.
  • the magnetic stirrer for this purpose, the mixing element is equipped with permanent magnets, which are driven by a rotating magnetic field.
  • Another possibility is to guide a mixer shaft, which is mechanically coupled to a drive, through the container wall, wherein the passage opening in the container inner wall has to be laboriously sealed against the passage of contaminants.
  • the object of the invention is to propose a mixing system in which the drive does not affect the sterility of the container interior of the disposable container.
  • the object is achieved by a mixing system with a drive which is encapsulated and which is disposable after use together with the mixer shaft and the disposable container.
  • the invention comprises a mixing system composed of a disposable flexible-use disposable container and a mixing device, the mixing device comprising at least one sealed drive disposable after use together with the mixer shaft and the disposable container.
  • the encapsulation is preferably realized by embedding the drive in plastic. This prevents the entry of particles and other impurities.
  • the outlet of the mixer shaft from the capsule is preferably sealed by a seal. In this case, plastic seals, preferably lip seals or molded multi-component injection-molded seals are preferred. Such gaskets are impassable for gases, liquids and microorganisms.
  • the drive can be located both outside the container interior, as well as in the container interior.
  • the invention relates to designs with drives in which the mixer shaft is set either in rotation or in a linear up and down movement.
  • the drive can be either an electric motor or a pneumatically or hydraulically operated drive. Particularly advantageous is a hydraulic or pneumatic drive, because it is exclusively made of plastic, which has advantages for environmentally sound disposal.
  • the motor was sterilized prior to installation by treatment with electromagnetic radiation, by autoclaving or by the action of sterilizing media.
  • the mixing system can be used for mixing media as a bioreactor.
  • Figure 1 shows a schematic overview of a mixing system with a drive for single use, in which the drive is outside of the disposable container.
  • Figure 2 shows a schematic overview of a mixing system with a drive for single use, in which there is a rotating drive in the container interior.
  • Figure 3 shows a schematic overview of a mixing system with a drive for
  • the mixing system consists of a disposable container 1, which has a container interior 2.
  • a mixer shaft 3 with the mixing elements 4 and 4 '.
  • the mixer shaft 3 is set in a rotating movement by a drive 5, which is located outside the container interior 2.
  • a first bearing 8 is connected to the capsule 6 and a second bearing 9 is located at the bottom of the disposable container 1.
  • the mixing system consists of a disposable container 1 which has a container interior 2.
  • a mixer shaft 3 In the container interior 2 is a mixer shaft 3 with the mixing elements 4 and 4 '.
  • the mixer shaft 3 is driven by a drive 5 located in the
  • Container interior 2 is located, offset in a rotating movement.
  • the drive is surrounded by a capsule 6 made of plastic. Against the ingress of media from the
  • Container interior 2 the opening of the capsule to the container interior 2 is provided with a seal 7 which surrounds the mixer shaft 3.
  • a seal 7 which surrounds the mixer shaft 3.
  • a first bearing 8 is connected to the capsule 6 and a second bearing 9 is located at the bottom of the disposable container.
  • the mixing system consists of a disposable container 1, which has a container interior 2.
  • a mixer shaft 3 In the container interior is a mixer shaft 3 with a mixing element 4.
  • the mixer shaft 3 is by a drive 5, located in the
  • Container interior is offset in a linear up and down movement.
  • the drive is surrounded by a capsule 6 made of plastic. Against the ingress of media from the
  • Container interior 2 the opening of the capsule to the container interior 2 is provided with a lip seal 7 which surrounds the mixer shaft 3 or to the capsule 6 is additionally formed a flexible plastic bellows sealingly enclosing a portion of the mixer shaft below the capsule (not shown).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Accessories For Mixers (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)

Abstract

Système de mélange qui comporte un récipient à usage unique à parois souples et un dispositif de mélange à usage unique. Les systèmes de mélange connus comportent des systèmes compliqués d'étanchéification de l'orifice de passage de l'axe du mélangeur. L'objet de la présente invention est de résoudre avantageusement le problème du coût et du facteur de sécurité. Le système de mélange selon la présente invention est constitué d'au moins un récipient à usage unique (1) pourvu de parois souples et d'au moins un dispositif de mélange comportant au moins un dispositif d'entraînement (5) encapsulé et un axe de mélangeur (3), le dispositif d'entraînement pouvant être mis au rebut après utilisation, avec l'axe de mélangeur (3) et le récipient à usage unique (1). Le système de mélange selon la présente invention peut être utilisé de préférence dans le domaine de la biotechnologie et de l'industrie pharmaceutique pour mélanger des liquides, dissoudre des matières solides ou multiplier des cellules et des micro-organismes.
EP09753610A 2008-05-28 2009-05-07 Système de mélange et son utilisation Active EP2296795B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008025507A DE102008025507A1 (de) 2008-05-28 2008-05-28 Mischsystem
PCT/EP2009/003253 WO2009143955A1 (fr) 2008-05-28 2009-05-07 Système de mélange

Publications (2)

Publication Number Publication Date
EP2296795A1 true EP2296795A1 (fr) 2011-03-23
EP2296795B1 EP2296795B1 (fr) 2012-10-17

Family

ID=40911767

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09753610A Active EP2296795B1 (fr) 2008-05-28 2009-05-07 Système de mélange et son utilisation

Country Status (4)

Country Link
US (1) US9669366B2 (fr)
EP (1) EP2296795B1 (fr)
DE (2) DE102008025507A1 (fr)
WO (1) WO2009143955A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9700857B1 (en) 2012-03-23 2017-07-11 Life Technologies Corporation Fluid mixing system with drive shaft steady support

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DE102006020461B3 (de) * 2006-04-28 2007-10-04 Sartorius Biotech Gmbh Behälter mit flexiblen Wänden
DE102009056967B4 (de) 2009-12-07 2013-07-04 Sartorius Stedim Biotech Gmbh Mischvorrichtung
DE102010036193A1 (de) 2010-09-02 2012-03-08 Sartorius Stedim Biotech Gmbh Vorrichtung zur kontaktlosen Übertragung elektrischer Energie für Bioreaktorbauteile
EP2674480B2 (fr) 2012-06-15 2023-04-05 DASGIP Information and Process Technology GmbH Dispositif de raccordement pour la conduite de fluide stérile et à usage unique d'un bioréacteur jetable et procédé pour le traitement d'un flux de liquide
CN103975055B (zh) 2011-10-10 2016-05-04 德国达斯其普信息与程序技术有限公司 包括生物反应器的生物技术装置、用于生物反应器的排出气体温度控制装置以及用于处理生物技术装置中的排出气流的方法
US9643141B2 (en) 2011-10-27 2017-05-09 Trimr, Llc Shakeable container with agitator
WO2013151733A1 (fr) 2012-04-06 2013-10-10 Hyclone Laboratories, Inc. Système de mélange de fluide avec ligne d'entraînement flexible et roue à aubes pliable
EP2674479B2 (fr) 2012-06-15 2025-03-12 Eppendorf SE Bioréacteur jetable et plaque frontale, ainsi que procédés de fabrication
USD804247S1 (en) 2012-10-26 2017-12-05 Trimr, Llc Agitator on straw or rod for a shakable container
WO2014072577A1 (fr) * 2012-11-07 2014-05-15 Maa- Ja Elintarviketalouden Tutkimuskeskus Appareil de bioréacteur
PT2951452T (pt) * 2013-02-01 2016-11-04 Asociación Centro De Investigación Coop En Biomateriales Sistema de agitação não intrusivo
DE102013112255A1 (de) * 2013-11-07 2015-05-07 Agraferm Technologies Ag Rührwerksgestell und Rührwerksanordnung für Fermenter, Fermenter, Biogasanlage, Verfahren zum Erzeugen von Biogas und Verfahren zum Umrüsten eines Fermenters
CA2966764C (fr) 2014-11-06 2022-10-18 Merck Patent Gmbh Charbon actif pour l'elimination de substances lixiviables et/ou extractibles
ES2833357T3 (es) * 2015-11-02 2021-06-15 Megakaryon Corp Procedimiento de preparación de plaquetas con un dispositivo de agitación recíproca
CN113975988B (zh) 2015-12-29 2024-09-03 生命科技股份有限公司 具有侧向移位的柔性驱动线的流体混合系统及使用方法
CN107469672A (zh) * 2017-09-14 2017-12-15 苏州艾乐蒙特机电科技有限公司 一种叶片推进式搅拌器
US12139698B2 (en) * 2017-12-22 2024-11-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Axial dispersion bioreactor (ADBR) for production of microalgae and other microorganisms
CN111699240A (zh) 2018-01-17 2020-09-22 生命技术公司 用于细胞培养缩放的系统和方法
WO2020041762A1 (fr) 2018-08-24 2020-02-27 Cuello Joel L Systèmes de culture mobiles et modulaires pour l'agriculture verticale
DE102018007288A1 (de) * 2018-09-14 2020-03-19 Levitronix Gmbh Mischvorrichtung mit einem Rührelement und Mischvorrichtungssystem
CN118649589B (zh) * 2024-08-06 2024-10-25 云南中正化学工业有限公司 一种用于肥料生产的混料装置及混料方法

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9700857B1 (en) 2012-03-23 2017-07-11 Life Technologies Corporation Fluid mixing system with drive shaft steady support
US10118141B2 (en) 2012-03-23 2018-11-06 Life Technologies Corporation Fluid mixing system with steady support
US10850243B2 (en) 2012-03-23 2020-12-01 Life Technologies Corporation Fluid mixing system
US11944945B2 (en) 2012-03-23 2024-04-02 Life Technologies Corporation Fluid mixing systems and methods of use
US12558657B2 (en) 2012-03-23 2026-02-24 Life Technologies Corporation Fluid mixing systems and methods of use

Also Published As

Publication number Publication date
US20110058448A1 (en) 2011-03-10
DE102008025507A1 (de) 2009-12-03
EP2296795B1 (fr) 2012-10-17
DE202009006889U1 (de) 2009-07-30
WO2009143955A1 (fr) 2009-12-03
US9669366B2 (en) 2017-06-06

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