EP2319623A2 - Dispositif de dosage pour liquides et procédé de dosage de liquides - Google Patents

Dispositif de dosage pour liquides et procédé de dosage de liquides Download PDF

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Publication number
EP2319623A2
EP2319623A2 EP10013402A EP10013402A EP2319623A2 EP 2319623 A2 EP2319623 A2 EP 2319623A2 EP 10013402 A EP10013402 A EP 10013402A EP 10013402 A EP10013402 A EP 10013402A EP 2319623 A2 EP2319623 A2 EP 2319623A2
Authority
EP
European Patent Office
Prior art keywords
syringe
pipette tip
displacement
releasing
dosing device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10013402A
Other languages
German (de)
English (en)
Other versions
EP2319623A3 (fr
EP2319623B1 (fr
Inventor
Karl-Friedrich Andres
Peter Molitor
Peter Schmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP2319623A2 publication Critical patent/EP2319623A2/fr
Publication of EP2319623A3 publication Critical patent/EP2319623A3/fr
Application granted granted Critical
Publication of EP2319623B1 publication Critical patent/EP2319623B1/fr
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Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227—Details of motor drive means

Definitions

  • the invention relates to a metering device for liquids with means for holding a pipette tip or syringe and means for releasing the pipette tip or syringe and with an electric drive motor for moving a displacement member of a displacing means for sucking or expelling air into or out of a pipette tip or for moving of couplable with a syringe plunger of the syringe coupling means.
  • the means for releasing the pipette tip or syringe are actuated separately without the displacement element or the syringe piston and the coupling means coupled with it being moved.
  • the invention relates to a method for dosing liquids by means of such Dosiervornchtung.
  • Dosing devices are mainly used in the laboratory for dosing liquids.
  • the amounts of liquid to be metered are in particular in the range of 100 nl to a few 100 ml settled.
  • Dosing devices, with which liquid is taken up in a single step and delivered in a single step, are called pipettes.
  • Dosing devices, with which liquid is taken up in a single or several steps and dispensed in several steps, is called a dispenser.
  • Pipette tips are small tubes with an opening at the bottom and an opening at the top.
  • the lower opening is usually smaller than the upper opening.
  • Syringes have a syringe barrel and a syringe plunger movably mounted in the syringe barrel by displacement in the direction of the barrel axis. Furthermore, the syringe barrel in the syringe bottom has a Syringe opening, which is often located in a small tube protruding from the syringe bottom.
  • the pipette tip is held on the dosing device during dosing, so that the upper opening is in communicating connection with a displacement device of the dosing device.
  • the displacement device is usually a cylinder with a piston displaceable therein. By moving the plunger, an air cushion is displaced so that liquid is drawn in or out of the pipette tip through the lower opening.
  • the syringe is held on the metering device so that the syringe barrel is immovably coupled with respect to the metering device and the syringe plunger with coupling means of the metering device is mechanically coupled.
  • the coupling means By displacing the coupling means, the syringe plunger can be displaced in the syringe barrel to draw liquid into and out of the syringe barrel.
  • known metering devices For moving the displacing member or the coupling means, known metering devices comprise a manually driven drive mechanism or an electric drive motor.
  • a metering device with an electric drive motor is controlled by means of electrical control means and has an electrical power supply connected to the electrical control means and the electric drive motor (eg battery, battery or power supply).
  • the electrical control means are also electrically connected to input means.
  • metering steps eg, intake and delivery of liquid
  • the input means may in particular electrical buttons or Be a switch.
  • dosing devices with an electric drive motor often have a display device (eg an LCD display) for outputting information about the operating state of the dosing device and / or the progress of a dosing procedure and / or selected dosing quantities and / or a selected operating mode.
  • a display device eg an LCD display
  • the means for holding the pipette tip are mostly guided as a press fit (e.g., clip-on or blind bore).
  • a passage opening in the front side of the attachment lug or in the bottom of the blind bore communicates with the displacement chamber of the displacement device.
  • the means for holding the syringe are, for example, cylinder gripping levers, which are associated with a cylinder receptacle in a housing of the metering device, in order to hold a flange on the syringe cylinder in the cylinder receptacle.
  • the means for holding may include piston gripping levers in a clutch bell into which a radially-protruded end of the syringe plunger or a syringe piston rod may be inserted to couple it to the clutch bell.
  • Pipette tips and syringes are detachably connected to the dosing device so that they can be replaced after use for fresh pipette tips or syringes. As a result, contamination can be avoided at subsequent dosages.
  • Single-use pipette tips or syringes are available inexpensively from plastic.
  • the means for releasing a pipette tip in many cases comprise a Abwerfschieber having an end which is associated with the attachment lug or the blind bore.
  • the other end of the discharge slide has an actuating button.
  • the means for releasing the pipette tip or syringe is preferably arranged on the outside of the body side and is not in communication with the displacement member of the displacement means, usually the cylinder, or the movable coupling means of the metering device when syringes are used.
  • the gripping levers can be pivoted outwards.
  • the Zylindergreifhebel may be provided with manually operable from the outside operating ends and having on the inside contact points which cooperate with actuating ends of the piston gripping lever, so that upon actuation of the Zylindergreifhebel the piston gripping levers are actuated when the syringe barrel is fully inserted into the syringe plunger.
  • the means for releasing a syringe may also comprise a mechanism which is actuated by means of a single actuating knob and acts on the Zylindergreifhebel and the piston gripping lever. In particular, irrespective of the position of the syringe plunger in the syringe barrel, the means for releasing the syringe can cause the syringe to be detached from the metering device.
  • the release of a pipette tip has no effect on the positioning of the piston in the cylinder of the displacement device. For example, if the dropping of the pipette tip occurs when the piston is in an upper position after the intake stroke, the piston must be displaced to a home position by additional actuation steps, from which a new complete metering operation can be started. If a metering device with an electric Drive motor after the release of the pipette tip with the piston in its upper position and then used by another person, there may be difficulties if this person does not recognize that the piston for the dosage must first be brought to a lower position to liquid with the dosing record.
  • the US Pat. No. 6,968,749 B2 discloses a portable automatic pipette with pipette tip ejector.
  • a sensor detects if a pipette tip has been removed.
  • a microprocessor controls the output of an alarm to alert the user that a misoperation has occurred.
  • the user For a new dosing process, the user must reset the pipette to the initial state.
  • the US Pat. No. 7,434,448 B2 describes a metering device with a dropper for pipette tips. It is also known from this document that the housing of the pipette has an upper housing part with a drive mechanism and a lower housing part detachably connectable with a displacement device. A plate on a piston rod of the piston is pressed under spring tension against the end of a drive rod of the drive mechanism.
  • a metering device with a housing top and a housing lower part and a coupling between the piston and the drive mechanism is known, which comprises an elastic element.
  • the US 5,620,660 describes a metering device with Zylindergreifhebeln and piston gripping levers for releasably holding syringes.
  • the object of the invention is to provide a metering device for liquids and a method for metering liquids, which facilitates the implementation of a further metering after the release of a pipette tip or syringe.
  • a displacement of the displacement member or the coupling means for receiving liquid in a pipette tip or syringe Preferably, from the basic position, a displacement of the displacement member or the coupling means for receiving liquid in a pipette tip or syringe.
  • the controllable drive is preferably an electric drive, in particular an electric drive motor.
  • the metering device has a base body, which is a supporting structure for further components of the metering device.
  • the main body is for example a housing.
  • the metering device can therefore be designed as a single-channel or as a multi-channel metering device.
  • the means for holding at least one pipette tip may be a clip-on lug or a blind bore.
  • the means for holding at least one syringe may include cylinder gripping levers and piston gripping levers.
  • the means for holding at least one pipette tip are present in combination with a displacement device with a displacement chamber and a displacement member.
  • the means for holding at least one syringe include coupling means which are mechanically coupled to the syringe plunger.
  • the coupling means may in particular be a clutch bell with piston gripping levers.
  • the means for releasing a pipette tip are separate from the displacement device and the coupling agent.
  • the means for releasing a pipette tip are preferably designed as Abwerfschieber which is arranged and arranged so that it pushes a pipette tip of a press fit, when the actuator connected to the Abwerfschieber is actuated.
  • the means for releasing a syringe have, for example, a mechanism that is in operative connection with Zylindergreifhebeln and piston gripping levers.
  • the actuation of the means for releasing is detected by a sensor which is arranged on the base body.
  • Electric control means controls the driveable actuator to displace the displacer or coupling means to a home position when the sensor detects actuation of the disengagement means.
  • the basic position can be chosen arbitrarily on the way, can be moved on the displacement member or coupling agent in the metering device.
  • the home position is preferably the position from which a displacement of the displacer or the coupling means is always upward when the intake of liquid into a fresh pipette tip or syringe is intended.
  • the sensor detects that the means for releasing have been actuated. This indirectly detects that no pipette tip or syringe is held on the metering device.
  • the signal from the sensor is used by the control means to drive the displacer or coupling means to the home position.
  • the metering device immediately after releasing the pipette tip or syringe, the metering device is again ready to receive liquid into a new pipette tip or syringe.
  • the basic position may, however, also be at the top, for example, so that the displacing member or coupling agent first has to be moved downwards before picking up a liquid. This can either be done automatically if the user gives a command to the dosing device to take up liquid by means of the input means. On the other hand, however, the user may also have to give an instruction to the dosing device to drive the displacement means or the coupling means downwards before picking up a liquid by input means.
  • the operation of the metering device is facilitated for the user.
  • the user is particularly protected against incorrect filling of the pipette tip or syringe and other errors.
  • the sensor disposed on the main body can also detect the disengagement of a pipette tip or syringe, e.g. in which he states that the sealed connection between the pipette tip and the means arranged on the main body for holding no longer exists.
  • the sensor may, for example, be a switch actuated by the means for releasing when the actuator is confirmed. In particular, this may be a switch which is arranged in the displacement region of the actuating member.
  • the sensor is a reed contact, which can be actuated by displacement of a magnet arranged on the means for releasing.
  • the magnet is preferably a permanent magnet.
  • the magnet is arranged on the actuating member.
  • the reed contact is arranged on a circuit board in the main body.
  • the board may be a board comprising the electrical control means or a part thereof.
  • any other sensor which detects the movement of the actuator or of the means for releasing the pipette tip or syringe.
  • the sensor detects the operation of the means for releasing in a position of the actuator or means for releasing, in which it is ensured that the pipette tip or syringe has been dropped.
  • the senor is responsive to an actuation of the means for releasing when the displacement of the actuator at least the Displacement path of the actuator, which must be returned for releasing the pipette tip or syringe from the means for holding.
  • the displacement path for actuating the sensor is 3.6 to 4.5 mm, preferably 4 mm, and the displacement path for releasing the pipette tip or syringe 2.5 to 3.5 mm, preferably 3.1 mm.
  • the means for holding at least one clamping seat for a pipette tip and the means for releasing a movable relative to the clamping seat Abwerfschiebers with one end for pressing a pipette tip from the clamping seat and the actuating member at the other end.
  • the displacement chamber is a cylinder and the displacement member is a piston arranged displaceably within the cylinder.
  • control means are arranged to, when the sensor detects an actuation of the means for releasing displace the displacing member to a home position, from which the extent of further displacement of the displacing member is determined, and the displacement member after reaching shift the home position to the home position.
  • the home position (also called “home position") serves to set the metering device to a defined starting position. From the home position, the displacements of the repressive organ are determined. This ensures that the extent of the displacement of the displacement element and thus the dosing accuracy can be controlled very accurately. This is particularly advantageous if the electric drive motor is a DC motor whose rotation is determined by means of a rotary encoder.
  • control means are arranged so that they control a movement of the displacement member in the home position and then in the home position when switching on the metering device. As a result, it is ensured each time the dosing device is switched on that the displacement of the displacement element takes place from a defined starting position.
  • the home position may already be the basic position into which the displacement element or the coupling means are displaced.
  • the home position can be realized, for example, in that the displacement element or an associated drive mechanism encounters a stop. This can lead to blockages in the drive system, increased power consumption and material damage.
  • a further sensor arranged in the main body which is electrically connected to the control means, detects the reaching of the home position by the displacement member.
  • the further sensor is a Hall sensor, the one with the displacer associated further magnet is actuated when the displacer reaches the home position.
  • the further magnet can also be connected indirectly to the displacement member, for example by being fixed to a drive mechanism which drives the displacement member.
  • the home position is 1 to 8 mm away from the home position, preferably 4 mm.
  • the control means are such that, starting from the basic position, they control an intake stroke of the displacement member, then at least one blow-out stroke until the basic position is reached by the displacement member and thereafter an overblow stroke until a blow-over position is reached.
  • the blow-over position is preferably arranged between the basic position and the home position. For example, it has a distance of 1.0 to 3.9 mm from the home position, in particular 3 mm.
  • the displacer can be moved very quickly in the fürblasposition to blow liquid from the pipette tip.
  • the displacement member can be moved very slowly and thus exactly in the home position. In principle, it is also possible that Sprintblasposition and home position coincide.
  • a defined initial position is given by the fact that the syringe piston impinges on the bottom of the syringe barrel. This impact is also desirable so that an air cushion between the syringe plunger and liquid is avoided. As a result, it is dispensable in this type of metering device, the Shifting agent and thus the syringe plunger in a home position. In this type of metering device, the metering operations thus preferably take place starting from a basic position in which the syringe piston is seated on the bottom of the syringe barrel.
  • the metering device is preferably a manageable metering device.
  • the housing is designed as a handle.
  • the metering device can also be designed as a stationary metering device. It is also possible to design the metering device so that it can be used as a manageable metering device and as a stationary metering device.
  • the displacement element or the coupling agent for receiving liquid is then displaced from the basic position into the pipette tip or syringe.
  • the displacement element is first moved to a home position and then to a basic position.
  • this embodiment serves to carry out the metering operations from a defined starting point.
  • the displacement member when switching the metering device initially moved to the home position and then to the home position.
  • the displacement member for receiving liquid in the pipette tip from the basic position by at least one intake stroke then shifted by at least one Ausblashub until reaching the home position and then by an Automatblashub until reaching the Studentsblasposition.
  • top refers to the orientation of the metering device as shown in the figures.
  • a pipette tip held on the metering device is arranged with its lower opening at the bottom to receive liquid from a vessel located below the metering device or to discharge it into such a vessel.
  • the metering device 1 has an elongated, designed as a handle housing 2 with an upper housing part 2.1 and a lower housing part 2.2.
  • the upper housing part 2.1 has input means 3 in the form of a rocker switch 3.1 and two buttons 3.2, 3.3 and a mode rotary switch 3.3.
  • a display device 4 arranged in the form of an LCD display.
  • the lower housing part 2.2 has at the lower end a conical clamping seat 5 for attaching a pipette tip 6.
  • the metering device has means for releasing 7 the pipette tip 6 from the clamping seat.
  • These means for releasing 7 include a Abwerfschieber 7.1, which has a sleeve-shaped slide part 7.2, which is arranged on the lower housing part 2.2 and from an upper abutment position on the upper housing part 2.1 from below to be displaced.
  • the Abwerfschieber 7.1 comprises a rod part 7.3, which is guided in a vertical guide channel in the upper housing part 2.1.
  • the rod part 7.3 is connected at the upper end with an actuator 7.4 in the form of an actuating knob which is actuated from the outside.
  • a displacement device 12 is arranged with a displacement chamber 13 in the form of a cylinder and a displaceable member 14 movably arranged therein in the form of a piston.
  • the displacement chamber 13 is connected via a passage 14.1 with a passage opening 15 in the lower end face of the clamping seat fifth communicatively connected.
  • Fig. 1b shows a detailed embodiment of the housing lower part 2.2, which can be used in the metering device 1.
  • the above reference numerals are in Fig. 1b entered.
  • the lower housing part 2.2 is in the German patent application DE 10 2008 048 252.8 and the US Provisional 61/097393 described. The related description is incorporated by reference into the present application.
  • a sensor 16 is fixed in the form of a reed contact.
  • the reed contact is associated with a magnet 17, which is designed as a cylindrical permanent magnet and is fixed at the upper end of the rod part 7.3 of Abwerfschiebers 7.1.
  • the sensor 16 is seated on the lower board portion 18.1 of a board 18, which has three with respect to each other angled platinum parts.
  • the upper board part 18.3 carries the display device 4 and the mode rotary switch 3.3. The reed contact is wired to the electrical control means 19.
  • an electric drive motor 20 is arranged in the upper housing part 2.1.
  • the drive motor 20 is a DC motor.
  • the drive motor 20 is arranged vertically with the shaft 21 at the lower end.
  • the shaft 21 carries a drive pinion 22.
  • Below the drive pinion 22, the shaft 21 carries in a bell-like socket 23 a Drehwinkelgeber-magnet 24. This is a permanent magnet, which is designed as a bar magnet.
  • rotary encoder 24 Below the rotary encoder 24 is arranged on a further circuit board as an IC rotary encoder 25 is arranged.
  • the rotary encoder 25 is wired to electrical control means 19.
  • a vertically aligned spindle 26 is arranged in the upper housing part 2.1.
  • the spindle 26 extends through a spindle nut 27 having a gear 28 on the outside.
  • the spindle nut 27 is mounted on both sides in ball bearings 29.1, 29.2.
  • the spindle 26 has at the top a radial projection 30 which is guided in an axially extending groove 31 of a spindle 26 parallel to the housing, fixed guide 32.
  • the projection 30 thus forms an anti-rotation of the spindle 26th
  • a further magnet 34 is positioned in the spindle 26 next to the projection 30.
  • This is also a permanent magnet, which is designed as a bar magnet.
  • a Hall sensor 36 is arranged in the upper housing part 2.1 on a further board 35.
  • the Hall sensor 36 is also connected to the electrical control means 19.
  • the spindle 26 is adjustable so that the further magnet 34 comes into the sphere of influence of the Hall sensor 36.
  • the spindle 26 carries at the lower end of a magnetic disk 37th
  • the displacement member 14 carries a piston rod 38 at the top, which carries a further magnetic plate 39 at the upper end.
  • the magnetic discs 37, 39 of the spindle 26 and the piston rod 38 have different poles on the sides facing each other so that they adhere to each other when they are brought into contact.
  • the further magnetic disk 38 can be pressed by a arranged in the lower housing part 2.2 spring 38.1 up to an upper stop 38.2 upwards.
  • the magnetic disk 37 is inserted through a hole 38.3 in the stop 38.2 through the magnetic disk 39.
  • Fig. 1 to 3 is a pipette tip 6 attached to the clamping seat 5 and the Abwerfschieber 7.1 not operated. In this configuration, the dosing device 1 is ready for a dosing process.
  • the spindle 26 is in a home position, in which the further magnet 34 is associated with the Hall sensor 36.
  • the electrical control means 19 controls the drive motor 20 so that the spindle 26 is brought into this position when the metering device 1 is turned on, by pressing an on / off switch, not shown.
  • the pulses of the rotary encoder 25 are counted by the electrical control means 19, starting from the home position in the home position.
  • the displacement of the spindle 26 is thus related to the home position.
  • the electrical control means 19 automatically controls the drive motor 20 so that the spindle 26 is displaced to a home position which is slightly above the home position. From the basic position, dosing operations are started in all operating modes, for example pipetting or dispensing.
  • the basic position is in the example 4 mm above the home position. This is in the FIGS. 9 and 10 shown.
  • the displacement of the spindle 26 from the basic position upward for carrying out a suction stroke is controlled by operating the switching rocker 3.1.
  • the metering device 1 After discharge of the actuator 7.4, the metering device 1 is ready for receiving a further pipette tip 6.
  • the inclusion of a further pipette tip 6 takes place by inserting the clamping seat 5 in the upper opening of the pipette tip. 6

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Human Computer Interaction (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP10013402.2A 2009-10-30 2010-10-07 Dispositif de dosage pour liquides et procédé de dosage de liquides Active EP2319623B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009051654A DE102009051654B4 (de) 2009-10-30 2009-10-30 Dosiervorrichtung für Flüssigkeiten und Verfahren zum Dosieren von Flüssigkeiten
US26188409P 2009-11-17 2009-11-17

Publications (3)

Publication Number Publication Date
EP2319623A2 true EP2319623A2 (fr) 2011-05-11
EP2319623A3 EP2319623A3 (fr) 2012-12-12
EP2319623B1 EP2319623B1 (fr) 2014-03-05

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ID=43828842

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10013402.2A Active EP2319623B1 (fr) 2009-10-30 2010-10-07 Dispositif de dosage pour liquides et procédé de dosage de liquides

Country Status (5)

Country Link
US (1) US8570029B2 (fr)
EP (1) EP2319623B1 (fr)
JP (1) JP5509033B2 (fr)
CN (1) CN102116654B (fr)
DE (1) DE102009051654B4 (fr)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
IT201900013110A1 (it) * 2019-07-26 2021-01-26 Giuseppe Zucca Apparato e metodo per il dosaggio gravimetrico di sostanze liquide

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WO2013166203A2 (fr) * 2012-05-01 2013-11-07 Life Technologies Corporation Dispositifs de pipette, systèmes et leurs procédés d'utilisation
EP3088904A1 (fr) * 2015-04-27 2016-11-02 Siemens Healthcare Diagnostics Products GmbH Procédé de vérification de la capacité de fonctionnement d'une station de lavage pour des aiguilles de prélèvement
EP3096148B1 (fr) * 2015-05-20 2024-01-03 Siemens Healthcare Diagnostics Products GmbH Dispositif de pipetage
EP3335795B1 (fr) * 2016-12-16 2019-03-27 Eppendorf AG Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides
US11980882B2 (en) 2017-02-03 2024-05-14 Eppendorf Se Handheld fluid transfer apparatus, laboratory system comprising the handheld fluid transfer apparatus, and method for operating a handheld fluid transfer apparatus or the laboratory system
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US8570029B2 (en) 2013-10-29
EP2319623A3 (fr) 2012-12-12
DE102009051654B4 (de) 2013-01-03
CN102116654B (zh) 2014-10-01
DE102009051654A1 (de) 2011-05-05
US20110181272A1 (en) 2011-07-28
JP2011121047A (ja) 2011-06-23
CN102116654A (zh) 2011-07-06
EP2319623B1 (fr) 2014-03-05
JP5509033B2 (ja) 2014-06-04

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