EP2456877A4 - Procédé d'édition de génome - Google Patents

Procédé d'édition de génome

Info

Publication number
EP2456877A4
EP2456877A4 EP20100803004 EP10803004A EP2456877A4 EP 2456877 A4 EP2456877 A4 EP 2456877A4 EP 20100803004 EP20100803004 EP 20100803004 EP 10803004 A EP10803004 A EP 10803004A EP 2456877 A4 EP2456877 A4 EP 2456877A4
Authority
EP
European Patent Office
Prior art keywords
genome editing
editing method
genome
editing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20100803004
Other languages
German (de)
English (en)
Other versions
EP2456877A1 (fr
Inventor
Edward Weinstein
Xiaoxia Cui
Phil Simmons
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/842,991 external-priority patent/US20110023152A1/en
Priority claimed from US12/842,978 external-priority patent/US20110023149A1/en
Priority claimed from US12/842,204 external-priority patent/US20110023159A1/en
Priority claimed from US12/842,993 external-priority patent/US20110023153A1/en
Priority claimed from US12/842,217 external-priority patent/US20110023141A1/en
Priority claimed from US12/842,886 external-priority patent/US20110023157A1/en
Priority claimed from US12/842,976 external-priority patent/US20120159653A1/en
Priority claimed from US12/843,000 external-priority patent/US20120159654A1/en
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Priority claimed from US12/842,893 external-priority patent/US20110016546A1/en
Priority claimed from US12/842,980 external-priority patent/US20110023150A1/en
Priority claimed from US12/842,999 external-priority patent/US20110016543A1/en
Priority claimed from US12/842,198 external-priority patent/US20110023139A1/en
Priority claimed from US12/842,269 external-priority patent/US20110023154A1/en
Priority claimed from US12/842,208 external-priority patent/US20110023140A1/en
Priority claimed from US12/842,188 external-priority patent/US20110023158A1/en
Priority claimed from US12/842,982 external-priority patent/US20110023151A1/en
Priority claimed from US12/842,719 external-priority patent/US20110016541A1/en
Priority claimed from US12/842,713 external-priority patent/US20110023147A1/en
Priority claimed from US12/842,219 external-priority patent/US20110023156A1/en
Priority claimed from US12/842,708 external-priority patent/US20110016540A1/en
Priority claimed from US12/842,839 external-priority patent/US20110016542A1/en
Priority claimed from US12/842,620 external-priority patent/US20110016539A1/en
Priority claimed from US12/842,578 external-priority patent/US20110023143A1/en
Priority claimed from US12/842,897 external-priority patent/US20110023148A1/en
Priority claimed from US12/842,678 external-priority patent/US20110023145A1/en
Priority claimed from US12/842,666 external-priority patent/US20110023144A1/en
Priority claimed from US12/842,694 external-priority patent/US20110023146A1/en
Priority claimed from US12/842,994 external-priority patent/US20110030072A1/en
Publication of EP2456877A4 publication Critical patent/EP2456877A4/fr
Publication of EP2456877A1 publication Critical patent/EP2456877A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/103—Ovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/107—Rabbit
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP10803004A 2009-07-24 2010-07-23 Procédé d'édition de génome Withdrawn EP2456877A1 (fr)

Applications Claiming Priority (41)

Application Number Priority Date Filing Date Title
US22841909P 2009-07-24 2009-07-24
US23262009P 2009-08-10 2009-08-10
US24587709P 2009-09-25 2009-09-25
US26369609P 2009-11-23 2009-11-23
US26390409P 2009-11-24 2009-11-24
US33600010P 2010-01-14 2010-01-14
US30808910P 2010-02-25 2010-02-25
US30972910P 2010-03-02 2010-03-02
US32371910P 2010-04-13 2010-04-13
US32369810P 2010-04-13 2010-04-13
US32370210P 2010-04-13 2010-04-13
US34328710P 2010-04-26 2010-04-26
US12/842,993 US20110023153A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in alzheimer's disease
US12/842,217 US20110023141A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved with parkinson's disease
US12/842,886 US20110023157A1 (en) 2008-12-04 2010-07-23 Equine genome editing with zinc finger nucleases
US12/842,976 US20120159653A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in macular degeneration
US12/843,000 US20120159654A1 (en) 2008-12-04 2010-07-23 Genome editing of genes involved in adme and toxicology in animals
US12/842,982 US20110023151A1 (en) 2008-12-04 2010-07-23 Genome editing of abc transporters
US12/842,893 US20110016546A1 (en) 2008-12-04 2010-07-23 Porcine genome editing with zinc finger nucleases
US12/842,980 US20110023150A1 (en) 2008-12-04 2010-07-23 Genome editing of genes associated with schizophrenia in animals
US12/842,999 US20110016543A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in inflammation
US12/842,198 US20110023139A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in cardiovascular disease
US12/842,269 US20110023154A1 (en) 2008-12-04 2010-07-23 Silkworm genome editing with zinc finger nucleases
US12/842,188 US20110023158A1 (en) 2008-12-04 2010-07-23 Bovine genome editing with zinc finger nucleases
US12/842,719 US20110016541A1 (en) 2008-12-04 2010-07-23 Genome editing of sensory-related genes in animals
US12/842,991 US20110023152A1 (en) 2008-12-04 2010-07-23 Genome editing of cognition related genes in animals
US12/842,978 US20110023149A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in tumor suppression in animals
US12/842,713 US20110023147A1 (en) 2008-12-04 2010-07-23 Genomic editing of prion disorder-related genes in animals
US12/842,219 US20110023156A1 (en) 2008-12-04 2010-07-23 Feline genome editing with zinc finger nucleases
US12/842,708 US20110016540A1 (en) 2008-12-04 2010-07-23 Genome editing of genes associated with trinucleotide repeat expansion disorders in animals
US12/842,839 US20110016542A1 (en) 2008-12-04 2010-07-23 Canine genome editing with zinc finger nucleases
US12/842,620 US20110016539A1 (en) 2008-12-04 2010-07-23 Genome editing of neurotransmission-related genes in animals
US12/842,578 US20110023143A1 (en) 2008-12-04 2010-07-23 Genomic editing of neurodevelopmental genes in animals
US12/842,897 US20110023148A1 (en) 2008-12-04 2010-07-23 Genome editing of addiction-related genes in animals
US12/842,678 US20110023145A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in autism spectrum disorders
PCT/US2010/043167 WO2011011767A1 (fr) 2009-07-24 2010-07-23 Procédé d'édition de génome
US12/842,666 US20110023144A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in amyotrophyic lateral sclerosis disease
US12/842,694 US20110023146A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in secretase-associated disorders
US12/842,994 US20110030072A1 (en) 2008-12-04 2010-07-23 Genome editing of immunodeficiency genes in animals
US12/842,208 US20110023140A1 (en) 2008-12-04 2010-07-23 Rabbit genome editing with zinc finger nucleases
US12/842,204 US20110023159A1 (en) 2008-12-04 2010-07-23 Ovine genome editing with zinc finger nucleases

Publications (2)

Publication Number Publication Date
EP2456877A4 true EP2456877A4 (fr) 2012-05-30
EP2456877A1 EP2456877A1 (fr) 2012-05-30

Family

ID=43499447

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10803004A Withdrawn EP2456877A1 (fr) 2009-07-24 2010-07-23 Procédé d'édition de génome

Country Status (8)

Country Link
EP (1) EP2456877A1 (fr)
JP (1) JP2013500018A (fr)
KR (1) KR20120097483A (fr)
AU (1) AU2010275432A1 (fr)
CA (1) CA2767377A1 (fr)
IL (1) IL217409A0 (fr)
SG (1) SG177711A1 (fr)
WO (1) WO2011011767A1 (fr)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2769262C (fr) * 2009-07-28 2019-04-30 Sangamo Biosciences, Inc. Procedes et compositions de traitement de troubles de repetition tri-nucleotidique
PL2566972T3 (pl) * 2010-05-03 2020-06-29 Sangamo Therapeutics, Inc. Kompozycje do wiązania modułów z motywem palca cynkowego
BR112013001685B1 (pt) 2010-07-23 2021-10-13 Sigma-Aldrich Co. Llc Método para editar pelo menos uma sequência cromossômica endógena em uma célula pela inserção de uma sequência na sequência cromossômica
BR112013021785A8 (pt) * 2011-02-25 2018-07-03 Recombinetics Inc animais geneticamente modificados e métodos para fazer os mesmos
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
CN102296073B (zh) * 2011-08-11 2014-04-23 中国农业科学院北京畜牧兽医研究所 锌指核酸酶定点敲除Myostatin基因特异靶位点
US9150847B2 (en) * 2011-09-21 2015-10-06 Sangamo Biosciences, Inc. Methods and compositions for regulation of transgene expression
US8895264B2 (en) 2011-10-27 2014-11-25 Sangamo Biosciences, Inc. Methods and compositions for modification of the HPRT locus
RU2691027C2 (ru) 2011-12-05 2019-06-07 Фэктор Байосайенс Инк. Способы и препараты для трансфекции клеток
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
WO2013112595A2 (fr) * 2012-01-23 2013-08-01 Sangamo Biosciences, Inc. Procédés et compositions pour l'édition génique d'un pathogène
CN102660642A (zh) * 2012-04-24 2012-09-12 西北农林科技大学 一种筛选锌指蛋白的筛选系统
JP6559063B2 (ja) * 2012-05-07 2019-08-14 サンガモ セラピューティクス， インコーポレイテッド 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
MX2014015204A (es) * 2012-06-12 2015-08-07 Genentech Inc Metodos y composiciones para generar alelos con inactivacion condicional.
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US10648001B2 (en) 2012-07-11 2020-05-12 Sangamo Therapeutics, Inc. Method of treating mucopolysaccharidosis type I or II
CA2875618C (fr) 2012-07-11 2021-04-27 Sangamo Biosciences, Inc. Methodes et compositions pour le traitement de maladies lysosomales
AP2015008495A0 (en) * 2012-10-30 2015-05-31 Ct For Aquaculture Technologies Inc Control of sexual maturation in animals
US9255250B2 (en) * 2012-12-05 2016-02-09 Sangamo Bioscience, Inc. Isolated mouse or human cell having an exogenous transgene in an endogenous albumin gene
EP3135765A1 (fr) 2012-12-06 2017-03-01 Sigma-Aldrich Co. LLC Modification et régulation de génome à base de crispr
WO2014093701A1 (fr) 2012-12-12 2014-06-19 The Broad Institute, Inc. Génomique fonctionnelle employant des systèmes crispr-cas, des compositions, des procédés, des banques d'inactivation et leurs applications
IL239317B (en) 2012-12-12 2022-07-01 Broad Inst Inc Providing, engineering and optimizing systems, methods and compositions for sequence manipulation and therapeutic applications
EP3434776A1 (fr) 2012-12-12 2019-01-30 The Broad Institute, Inc. Procédés, modèles, systèmes et appareil pour identifier des séquences cibles pour les enzymes cas ou des systèmes crispr-cas pour des séquences cibles et transmettre les résultats associés
US20140235933A1 (en) 2013-02-20 2014-08-21 Regeneron Pharmaceuticals, Inc. Genetic modification of rats
SG11201507378UA (en) 2013-03-14 2015-10-29 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
EP2975942B1 (fr) * 2013-03-21 2018-08-08 Sangamo Therapeutics, Inc. Interruption ciblée de gènes de récepteur des lymphocytes t à l'aide de nucléases protéiques à doigt de zinc génétiquement modifiées
AU2014247151A1 (en) 2013-04-02 2015-10-08 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
DK2986729T3 (en) 2013-04-16 2018-10-29 Regeneron Pharma TARGETED MODIFICATION OF ROOT THROUGH
CA2910427C (fr) * 2013-05-10 2024-02-20 Sangamo Biosciences, Inc. Procedes et compositions d'apport pour genie genomique medie par nuclease
EP3730615A3 (fr) * 2013-05-15 2020-12-09 Sangamo Therapeutics, Inc. Procédés et compositions pour le traitement d'une maladie génétique
US20140359796A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
MX2015017312A (es) 2013-06-17 2017-04-10 Broad Inst Inc Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas.
CN105793425B (zh) 2013-06-17 2021-10-26 布罗德研究所有限公司 使用病毒组分靶向障碍和疾病的crispr-cas系统和组合物的递送、用途和治疗应用
JP6665088B2 (ja) 2013-06-17 2020-03-13 ザ・ブロード・インスティテュート・インコーポレイテッド 配列操作のための最適化されたＣＲＩＳＰＲ−Ｃａｓ二重ニッカーゼ系、方法および組成物
WO2014204724A1 (fr) 2013-06-17 2014-12-24 The Broad Institute Inc. Administration, modification et optimisation de systèmes guides tandems, méthodes et compositions pour la manipulation de séquence
EP3725885A1 (fr) 2013-06-17 2020-10-21 The Broad Institute, Inc. Génomique fonctionnelle utilisant des systèmes crispr-cas, compositions, procédés, cribles et applications de ces systèmes
EP3865575A1 (fr) * 2013-11-06 2021-08-18 Hiroshima University Vecteur d'insertion d'acide nucléique
US9951353B2 (en) * 2013-11-15 2018-04-24 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Engineering neural stem cells using homologous recombination
JP6300302B2 (ja) * 2013-11-27 2018-03-28 国立研究開発法人農業・食品産業技術総合研究機構 カイコのセリシン１変異系統
CN111206032B (zh) 2013-12-12 2024-07-19 布罗德研究所有限公司 用于基因组编辑的crispr-cas系统和组合物的递送、用途和治疗应用
WO2015089486A2 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Systèmes, procédés et compositions pour manipulation de séquences avec systèmes crispr-cas fonctionnels optimisés
RU2016128077A (ru) 2013-12-12 2018-12-06 Те Брод Инститьют Инк. Доставка, применение и применения в терапии систем и композиций crispr-cas для лечения обусловленных hbv и вирусных заболеваний и нарушений
BR112016013547A2 (pt) 2013-12-12 2017-10-03 Broad Inst Inc Composições e métodos de uso de sistemas crispr-cas em distúrbios de repetições de nucleotídeos
WO2015089364A1 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Structure cristalline d'un système crispr-cas, et ses utilisations
WO2015089473A1 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Ingénierie de systèmes, procédés et compositions guides optimisées avec de nouvelles architectures pour la manipulation de séquences
US20170002413A1 (en) * 2014-01-17 2017-01-05 The University Of Tokyo Molecule associated with onset of gout, and method and kit for evaluating diathesis of uric acid-related diseases and inflammation-related diseases, and inspection object and drug
AU2015218576B2 (en) 2014-02-24 2020-02-27 Sangamo Therapeutics, Inc. Methods and compositions for nuclease-mediated targeted integration
NO2785538T3 (fr) * 2014-05-07 2018-08-04
CN104450887B (zh) * 2014-11-12 2017-07-25 兰州百源基因技术有限公司 用于检测子宫内膜癌的dna探针、基因芯片及其应用
EP3230451B1 (fr) 2014-12-12 2021-04-07 The Broad Institute, Inc. Arn guides protégés (pgrnas)
CA2970370A1 (fr) 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr presentant ou associe avec un domaine de destabilisation
WO2016112961A1 (fr) * 2015-01-13 2016-07-21 Institut National De La Sante Et De La Recherche Medicale (Inserm) Inactivations conditionnelles de caspase 2 et procédés associés
IL293323B2 (en) 2015-06-18 2024-01-01 Massachusetts Inst Technology Crispr enzyme mutations reducing off-target effects
WO2016205759A1 (fr) 2015-06-18 2016-12-22 The Broad Institute Inc. Modification et optimisation de systèmes, de méthodes, d'enzymes et d'échafaudages guides d'orthologues de cas9 et variant pour la manipulation de séquences
JP6813191B2 (ja) * 2015-07-31 2021-01-13 北海道公立大学法人 札幌医科大学 グリオーマの予後、遠隔部再発リスク及び浸潤を判定する方法及びキット並びにグリオーマを処置するための医薬組成物
WO2017152023A1 (fr) * 2016-03-04 2017-09-08 Indoor Biotechnologies Inc. Inactivations de fel d 1 et compositions et procédés associés basés sur l'édition génomique de crispr-cas9
ES3039670T3 (en) 2016-04-15 2025-10-23 Memorial Sloan Kettering Cancer Center Transgenic t cell and chimeric antigen receptor t cell compositions and related methods
CA3029132A1 (fr) 2016-07-06 2018-01-11 Vertex Pharmaceuticals Incorporated Materiaux et procedes de traitement de troubles lies a la douleur
US11459587B2 (en) * 2016-07-06 2022-10-04 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of pain related disorders
WO2018020050A1 (fr) * 2016-07-29 2018-02-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Diversification ciblée de protéine in situ par clivage et réparation d'adn dirigés
WO2018222667A1 (fr) * 2017-05-31 2018-12-06 22Nd Century Limited, Llc Procédés d'édition génomique pour produire des produits de tabac à faible teneur en nicotine
US20210062260A1 (en) * 2017-12-21 2021-03-04 Livestock Improvement Corporation Limited Genetic markers and uses therefor
US11179479B2 (en) * 2018-01-05 2021-11-23 Animatus Biosciences, Llc Enhanced cardiomyocyte regeneration
CN111684078B (zh) * 2018-02-12 2024-04-19 豪夫迈·罗氏有限公司 通过评价肿瘤遗传异质性来预测对治疗的应答的方法
CN108441577B (zh) * 2018-05-25 2021-05-14 兰州大学 引物对、试剂盒及用途和检测紫花苜蓿产品中是否含有大豆的方法
SG11202012499RA (en) 2018-06-28 2021-01-28 Crispr Therapeutics Ag Compositions and methods for genomic editing by insertion of donor polynucleotides
BR112021007301A2 (pt) 2018-10-18 2021-07-27 Intellia Therapeutics, Inc. composições e métodos para expressar fator ix
JP7756639B2 (ja) 2019-11-08 2025-10-20 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｘ連鎖性若年網膜分離療法のためのｃｒｉｓｐｒおよびａａｖ戦略
CN111073900B (zh) * 2019-12-09 2023-05-02 温氏食品集团股份有限公司 一种提高猪克隆胚胎发育效率的方法
CN111172274B (zh) * 2020-01-22 2021-10-15 清华大学 Synaptotagmin-7在双向情感障碍诊断和治疗中的用途
US12543712B2 (en) 2020-02-21 2026-02-10 University of Pittsburgh—of the Commonwealth System of Higher Education Genetically modified mouse with a disruption in an AANAT gene
CN111235153B (zh) * 2020-04-01 2023-05-26 陕西慧康生物科技有限责任公司 靶向敲除人MC1R基因的sgRNA及其构建的细胞株
CN111235154A (zh) * 2020-04-01 2020-06-05 陕西慧康生物科技有限责任公司 靶向敲除人MC1R基因的sgRNA及其构建的细胞株
WO2022145495A1 (fr) * 2021-01-04 2022-07-07 Modalis Therapeutics Corporation Procédé de traitement d'ataxies spinocérébelleuses (sca) par ciblage du gène atxn7
IL282597B (en) * 2021-04-22 2022-08-01 B G Negev Technologies And Applications Ltd At Ben Gurion Univ Birds for producing female hatchling and methods of producing same
CA3205739A1 (fr) * 2021-05-12 2022-11-17 Eva CZIRR Procedes et compositions pour traiter des troubles associes au vieillissement avec des modulateurs de l'element 2 de la famille du facteur en trefle
CN113355356A (zh) * 2021-07-05 2021-09-07 西北农林科技大学 一种csn1s1基因敲除载体及csn1s1基因敲除乳腺上皮细胞的制备方法
CN113881646B (zh) * 2021-09-28 2023-08-04 北京市农林科学院 参与植物抗病的相关蛋白TaFAH1及其基因和应用
CN113916754B (zh) * 2021-10-12 2023-11-10 四川大学华西医院 用于检测乳腺癌患者循环肿瘤细胞的细胞表面标志物及应用
KR20240143936A (ko) * 2023-03-21 2024-10-02 주식회사 키베이직 콧물 시료를 이용한 반려동물의 인지 장애 진단용 조성물
CN118943377B (zh) * 2024-07-18 2025-05-06 江阴纳力新材料科技有限公司 一种复合集流体及应用其的极片、二次电池
CN119372338B (zh) * 2024-12-11 2025-08-01 广东省农业科学院动物科学研究所 与番鸭繁殖性状相关的ulk1基因snp分子标记及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (fr) * 2002-01-23 2003-10-23 The University Of Utah Research Foundation Mutagenese chromosomique ciblee au moyen de nucleases en doigt a zinc

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203140A1 (en) * 2007-09-27 2009-08-13 Sangamo Biosciences, Inc. Genomic editing in zebrafish using zinc finger nucleases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (fr) * 2002-01-23 2003-10-23 The University Of Utah Research Foundation Mutagenese chromosomique ciblee au moyen de nucleases en doigt a zinc

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GEURTS ARON M ET AL: "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 325, no. 5939, 24 July 2009 (2009-07-24), pages 433, XP002580718, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1172447 *
MILLER JEFFREY C ET AL: "An improved zinc-finger nuclease architecture for highly specific genome editing", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 25, no. 7, 1 July 2007 (2007-07-01), pages 778 - 785, XP002465119, ISSN: 1087-0156, DOI: 10.1038/NBT1319 *
PORTEUS M H ET AL: "GENE TARGETING USING ZINC FINGER NUCLEASES", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 23, no. 8, 1 August 2005 (2005-08-01), pages 967 - 973, XP002467422, ISSN: 1087-0156, DOI: 10.1038/NBT1125 *
See also references of WO2011011767A1 *
URNOV F D ET AL: "Highly efficient endogenous human gene correction using designed zinc-finger nucleases", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 435, no. 7042, 2 June 2005 (2005-06-02), pages 646 - 651, XP002411069, ISSN: 0028-0836, DOI: 10.1038/NATURE03556 *

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JP2013500018A (ja) 2013-01-07
WO2011011767A1 (fr) 2011-01-27
SG177711A1 (en) 2012-02-28
KR20120097483A (ko) 2012-09-04
EP2456877A1 (fr) 2012-05-30

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