EP2456877A4 - Procédé d'édition de génome - Google Patents

Procédé d'édition de génome

Info

Publication number
EP2456877A4
EP2456877A4 EP20100803004 EP10803004A EP2456877A4 EP 2456877 A4 EP2456877 A4 EP 2456877A4 EP 20100803004 EP20100803004 EP 20100803004 EP 10803004 A EP10803004 A EP 10803004A EP 2456877 A4 EP2456877 A4 EP 2456877A4
Authority
EP
European Patent Office
Prior art keywords
genome editing
editing method
genome
editing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20100803004
Other languages
German (de)
English (en)
Other versions
EP2456877A1 (fr
Inventor
Edward Weinstein
Xiaoxia Cui
Phil Simmons
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/842,188 external-priority patent/US20110023158A1/en
Priority claimed from US12/842,713 external-priority patent/US20110023147A1/en
Priority claimed from US12/842,897 external-priority patent/US20110023148A1/en
Priority claimed from US12/842,893 external-priority patent/US20110016546A1/en
Priority claimed from US12/842,978 external-priority patent/US20110023149A1/en
Priority claimed from US12/842,999 external-priority patent/US20110016543A1/en
Priority claimed from US12/842,198 external-priority patent/US20110023139A1/en
Priority claimed from US12/842,208 external-priority patent/US20110023140A1/en
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Priority claimed from US12/842,694 external-priority patent/US20110023146A1/en
Priority claimed from US12/842,976 external-priority patent/US20120159653A1/en
Priority claimed from US12/842,269 external-priority patent/US20110023154A1/en
Priority claimed from US12/842,219 external-priority patent/US20110023156A1/en
Priority claimed from US12/842,982 external-priority patent/US20110023151A1/en
Priority claimed from US12/842,708 external-priority patent/US20110016540A1/en
Priority claimed from US12/843,000 external-priority patent/US20120159654A1/en
Priority claimed from US12/842,886 external-priority patent/US20110023157A1/en
Priority claimed from US12/842,666 external-priority patent/US20110023144A1/en
Priority claimed from US12/842,719 external-priority patent/US20110016541A1/en
Priority claimed from US12/842,620 external-priority patent/US20110016539A1/en
Priority claimed from US12/842,204 external-priority patent/US20110023159A1/en
Priority claimed from US12/842,991 external-priority patent/US20110023152A1/en
Priority claimed from US12/842,993 external-priority patent/US20110023153A1/en
Priority claimed from US12/842,217 external-priority patent/US20110023141A1/en
Priority claimed from US12/842,980 external-priority patent/US20110023150A1/en
Priority claimed from US12/842,578 external-priority patent/US20110023143A1/en
Priority claimed from US12/842,994 external-priority patent/US20110030072A1/en
Priority claimed from US12/842,839 external-priority patent/US20110016542A1/en
Priority claimed from US12/842,678 external-priority patent/US20110023145A1/en
Publication of EP2456877A1 publication Critical patent/EP2456877A1/fr
Publication of EP2456877A4 publication Critical patent/EP2456877A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/103—Ovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/107—Rabbit
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP20100803004 2009-07-24 2010-07-23 Procédé d'édition de génome Withdrawn EP2456877A4 (fr)

Applications Claiming Priority (41)

Application Number Priority Date Filing Date Title
US22841909P 2009-07-24 2009-07-24
US23262009P 2009-08-10 2009-08-10
US24587709P 2009-09-25 2009-09-25
US26369609P 2009-11-23 2009-11-23
US26390409P 2009-11-24 2009-11-24
US33600010P 2010-01-14 2010-01-14
US30808910P 2010-02-25 2010-02-25
US30972910P 2010-03-02 2010-03-02
US32371910P 2010-04-13 2010-04-13
US32369810P 2010-04-13 2010-04-13
US32370210P 2010-04-13 2010-04-13
US34328710P 2010-04-26 2010-04-26
US12/842,893 US20110016546A1 (en) 2008-12-04 2010-07-23 Porcine genome editing with zinc finger nucleases
US12/842,978 US20110023149A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in tumor suppression in animals
US12/842,999 US20110016543A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in inflammation
US12/842,198 US20110023139A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in cardiovascular disease
US12/842,208 US20110023140A1 (en) 2008-12-04 2010-07-23 Rabbit genome editing with zinc finger nucleases
US12/842,886 US20110023157A1 (en) 2008-12-04 2010-07-23 Equine genome editing with zinc finger nucleases
US12/842,694 US20110023146A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in secretase-associated disorders
US12/842,976 US20120159653A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in macular degeneration
US12/842,269 US20110023154A1 (en) 2008-12-04 2010-07-23 Silkworm genome editing with zinc finger nucleases
US12/842,219 US20110023156A1 (en) 2008-12-04 2010-07-23 Feline genome editing with zinc finger nucleases
US12/842,982 US20110023151A1 (en) 2008-12-04 2010-07-23 Genome editing of abc transporters
US12/843,000 US20120159654A1 (en) 2008-12-04 2010-07-23 Genome editing of genes involved in adme and toxicology in animals
US12/842,666 US20110023144A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in amyotrophyic lateral sclerosis disease
US12/842,188 US20110023158A1 (en) 2008-12-04 2010-07-23 Bovine genome editing with zinc finger nucleases
US12/842,713 US20110023147A1 (en) 2008-12-04 2010-07-23 Genomic editing of prion disorder-related genes in animals
US12/842,719 US20110016541A1 (en) 2008-12-04 2010-07-23 Genome editing of sensory-related genes in animals
US12/842,620 US20110016539A1 (en) 2008-12-04 2010-07-23 Genome editing of neurotransmission-related genes in animals
US12/842,204 US20110023159A1 (en) 2008-12-04 2010-07-23 Ovine genome editing with zinc finger nucleases
PCT/US2010/043167 WO2011011767A1 (fr) 2009-07-24 2010-07-23 Procédé d'édition de génome
US12/842,991 US20110023152A1 (en) 2008-12-04 2010-07-23 Genome editing of cognition related genes in animals
US12/842,993 US20110023153A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in alzheimer's disease
US12/842,217 US20110023141A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved with parkinson's disease
US12/842,980 US20110023150A1 (en) 2008-12-04 2010-07-23 Genome editing of genes associated with schizophrenia in animals
US12/842,578 US20110023143A1 (en) 2008-12-04 2010-07-23 Genomic editing of neurodevelopmental genes in animals
US12/842,994 US20110030072A1 (en) 2008-12-04 2010-07-23 Genome editing of immunodeficiency genes in animals
US12/842,839 US20110016542A1 (en) 2008-12-04 2010-07-23 Canine genome editing with zinc finger nucleases
US12/842,678 US20110023145A1 (en) 2008-12-04 2010-07-23 Genomic editing of genes involved in autism spectrum disorders
US12/842,708 US20110016540A1 (en) 2008-12-04 2010-07-23 Genome editing of genes associated with trinucleotide repeat expansion disorders in animals
US12/842,897 US20110023148A1 (en) 2008-12-04 2010-07-23 Genome editing of addiction-related genes in animals

Publications (2)

Publication Number Publication Date
EP2456877A1 EP2456877A1 (fr) 2012-05-30
EP2456877A4 true EP2456877A4 (fr) 2012-05-30

Family

ID=43499447

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20100803004 Withdrawn EP2456877A4 (fr) 2009-07-24 2010-07-23 Procédé d'édition de génome

Country Status (8)

Country Link
EP (1) EP2456877A4 (fr)
JP (1) JP2013500018A (fr)
KR (1) KR20120097483A (fr)
AU (1) AU2010275432A1 (fr)
CA (1) CA2767377A1 (fr)
IL (1) IL217409A0 (fr)
SG (1) SG177711A1 (fr)
WO (1) WO2011011767A1 (fr)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5866283B2 (ja) * 2009-07-28 2016-02-17 サンガモ バイオサイエンシーズ， インコーポレイテッド トリヌクレオチド反復疾患を治療するための方法および組成物
PT2566972T (pt) * 2010-05-03 2020-03-02 Sangamo Biosciences Inc Composições para a ligação de módulos de dedo de zinco
WO2012012738A1 (fr) 2010-07-23 2012-01-26 Sigma-Aldrich Co., Llc Modifications du génome à l'aide d'endonucléases de ciblage et d'acides nucléiques simples brins
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
BR112013021785A8 (pt) * 2011-02-25 2018-07-03 Recombinetics Inc animais geneticamente modificados e métodos para fazer os mesmos
CN102296073B (zh) * 2011-08-11 2014-04-23 中国农业科学院北京畜牧兽医研究所 锌指核酸酶定点敲除Myostatin基因特异靶位点
AU2012312260B2 (en) 2011-09-21 2017-08-31 Sangamo Therapeutics, Inc. Methods and compositions for regulation of transgene expression
JP6188703B2 (ja) * 2011-10-27 2017-08-30 サンガモ セラピューティクス， インコーポレイテッド Ｈｐｒｔ遺伝子座を修飾するための方法および組成物
KR102320571B1 (ko) 2011-12-05 2021-11-02 팩터 바이오사이언스 인크. 세포를 형질감염시키는 방법들 및 생성물들
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
WO2013112595A2 (fr) * 2012-01-23 2013-08-01 Sangamo Biosciences, Inc. Procédés et compositions pour l'édition génique d'un pathogène
CN102660642A (zh) * 2012-04-24 2012-09-12 西北农林科技大学 一种筛选锌指蛋白的筛选系统
AU2013259647B2 (en) 2012-05-07 2018-11-08 Corteva Agriscience Llc Methods and compositions for nuclease-mediated targeted integration of transgenes
EP3241902B1 (fr) 2012-05-25 2018-02-28 The Regents of The University of California Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn
CN104540382A (zh) * 2012-06-12 2015-04-22 弗·哈夫曼-拉罗切有限公司 用于产生条件性敲除等位基因的方法和组合物
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
DK3444342T3 (da) 2012-07-11 2020-08-24 Sangamo Therapeutics Inc Fremgangsmåder og sammensætninger til behandlingen af lysosomale aflejringssygdomme
US10648001B2 (en) 2012-07-11 2020-05-12 Sangamo Therapeutics, Inc. Method of treating mucopolysaccharidosis type I or II
CA2889502A1 (fr) * 2012-10-30 2014-05-08 Recombinetics, Inc. Regulation de la maturation sexuelle chez les animaux
WO2014089212A1 (fr) * 2012-12-05 2014-06-12 Sangamo Biosciences, Inc. Procédés et compositions pour la régulation de troubles métaboliques
EP3363902B1 (fr) 2012-12-06 2019-11-27 Sigma Aldrich Co. LLC Modification et régulation de génome à base de crispr
DK2931897T3 (en) 2012-12-12 2018-02-05 Broad Inst Inc CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS
EP2931899A1 (fr) 2012-12-12 2015-10-21 The Broad Institute, Inc. Génomique fonctionnelle employant des systèmes crispr-cas, des compositions, des procédés, des banques d'inactivation et leurs applications
WO2014093709A1 (fr) 2012-12-12 2014-06-19 The Broad Institute, Inc. Procédés, modèles, systèmes et appareil pour identifier des séquences cibles pour les enzymes cas ou des systèmes crispr-cas pour des séquences cibles et transmettre les résultats associés
MX2015010841A (es) 2013-02-20 2016-05-09 Regeneron Pharma Modificacion genetica de ratas.
MX374090B (es) 2013-03-14 2025-03-05 Caribou Biosciences Inc Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico.
JP6346266B2 (ja) * 2013-03-21 2018-06-20 サンガモ セラピューティクス， インコーポレイテッド 操作されたジンクフィンガータンパク質ヌクレアーゼを使用するｔ細胞受容体遺伝子の標的化された破壊
EP2981614A1 (fr) 2013-04-02 2016-02-10 Bayer CropScience NV Modification ciblée du génome dans des cellules eucaryotes
SI2986729T1 (sl) 2013-04-16 2019-02-28 Regeneron Pharmaceuticals, Inc. Ciljana sprememba genoma podgane
US10604771B2 (en) * 2013-05-10 2020-03-31 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
CN105683376A (zh) * 2013-05-15 2016-06-15 桑格摩生物科学股份有限公司 用于治疗遗传病状的方法和组合物
US20140359796A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
MX2015017312A (es) 2013-06-17 2017-04-10 Broad Inst Inc Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas.
EP3725885A1 (fr) 2013-06-17 2020-10-21 The Broad Institute, Inc. Génomique fonctionnelle utilisant des systèmes crispr-cas, compositions, procédés, cribles et applications de ces systèmes
KR20250012194A (ko) 2013-06-17 2025-01-23 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용
EP3011030B1 (fr) 2013-06-17 2023-11-08 The Broad Institute, Inc. Systèmes, procédés et compositions à double nickase crispr-cas optimisés, pour la manipulation de séquences
WO2014204724A1 (fr) 2013-06-17 2014-12-24 The Broad Institute Inc. Administration, modification et optimisation de systèmes guides tandems, méthodes et compositions pour la manipulation de séquence
EP3865575A1 (fr) * 2013-11-06 2021-08-18 Hiroshima University Vecteur d'insertion d'acide nucléique
WO2015073867A1 (fr) * 2013-11-15 2015-05-21 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Manipulation génétique de cellules souches neurales par recombinaison homologue
JP6300302B2 (ja) * 2013-11-27 2018-03-28 国立研究開発法人農業・食品産業技術総合研究機構 カイコのセリシン１変異系統
BR112016013207A2 (pt) 2013-12-12 2017-09-26 Massachusetts Inst Technology administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais
EP3080259B1 (fr) 2013-12-12 2023-02-01 The Broad Institute, Inc. Ingénierie de systèmes, procédés et compositions guides optimisées avec de nouvelles architectures pour la manipulation de séquences
WO2015089364A1 (fr) 2013-12-12 2015-06-18 The Broad Institute Inc. Structure cristalline d'un système crispr-cas, et ses utilisations
MX2016007325A (es) 2013-12-12 2017-07-19 Broad Inst Inc Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos.
AU2014361781B2 (en) 2013-12-12 2021-04-01 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物
CA2943109A1 (fr) * 2014-01-17 2015-07-23 Nariyoshi Shinomiya Molecule associee a l'apparition de la goutte, et procede et trousse pour evaluer la diathese de maladies associees a l'acide urique et de maladies associees a une inflammation, et objet d'examen et medicament
AU2015218576B2 (en) * 2014-02-24 2020-02-27 Sangamo Therapeutics, Inc. Methods and compositions for nuclease-mediated targeted integration
NO2785538T3 (fr) * 2014-05-07 2018-08-04
CN104450887B (zh) * 2014-11-12 2017-07-25 兰州百源基因技术有限公司 用于检测子宫内膜癌的dna探针、基因芯片及其应用
EP3889260A1 (fr) 2014-12-12 2021-10-06 The Broad Institute, Inc. Arn guides protégés (pgrnas)
WO2016106244A1 (fr) 2014-12-24 2016-06-30 The Broad Institute Inc. Crispr présentant ou associé avec un domaine de déstabilisation
WO2016112961A1 (fr) * 2015-01-13 2016-07-21 Institut National De La Sante Et De La Recherche Medicale (Inserm) Inactivations conditionnelles de caspase 2 et procédés associés
WO2016205759A1 (fr) 2015-06-18 2016-12-22 The Broad Institute Inc. Modification et optimisation de systèmes, de méthodes, d'enzymes et d'échafaudages guides d'orthologues de cas9 et variant pour la manipulation de séquences
TWI813532B (zh) 2015-06-18 2023-09-01 美商博得學院股份有限公司 降低脱靶效應的ｃｒｉｓｐｒ酶突變
WO2017022634A1 (fr) * 2015-07-31 2017-02-09 北海道公立大学法人札幌医科大学 Procédé et kit permettant d'évaluer un pronostic, un risque de récidive à distance et une invasion de gliomes ainsi que composition pharmaceutique pour traiter un gliome
CA3016571A1 (fr) * 2016-03-04 2017-09-08 Indoor Biotechnologies Inc. Inactivations de fel d 1 et compositions et procedes associes bases sur l'edition genomique de crispr-cas9
US11377637B2 (en) 2016-04-15 2022-07-05 Memorial Sloan Kettering Cancer Center Transgenic T cell and chimeric antigen receptor T cell compositions and related methods
JP7305534B2 (ja) 2016-07-06 2023-07-10 バーテックス ファーマシューティカルズ インコーポレイテッド 疼痛関連障害を処置するための物質及び方法
AU2017292173B2 (en) * 2016-07-06 2022-01-13 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of pain related disorders
WO2018020050A1 (fr) * 2016-07-29 2018-02-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Diversification ciblée de protéine in situ par clivage et réparation d'adn dirigés
US20200140875A1 (en) * 2017-05-31 2020-05-07 22Nd Century Limited, Llc Genome editing methods for producing low-nicotine tobacco products
WO2019125187A1 (fr) * 2017-12-21 2019-06-27 Livestock Improvement Corporation Limited Marqueurs génétiques et leurs utilisations
US11179479B2 (en) * 2018-01-05 2021-11-23 Animatus Biosciences, Llc Enhanced cardiomyocyte regeneration
CA3090951C (fr) * 2018-02-12 2023-10-17 F.Hoffmann-La Roche Ag Procede de prediction de reponse a une therapie par evaluation de l'heterogeneite genetique tumorale
CN108441577B (zh) * 2018-05-25 2021-05-14 兰州大学 引物对、试剂盒及用途和检测紫花苜蓿产品中是否含有大豆的方法
CN112585268B (zh) 2018-06-28 2025-03-04 克里斯珀医疗股份公司 通过插入供体多核苷酸用于基因组编辑的组合物和方法
JP7578590B2 (ja) 2018-10-18 2024-11-06 インテリア セラピューティクス，インコーポレーテッド 第ｉｘ因子を発現するための組成物及び方法
US12521451B2 (en) 2019-11-08 2026-01-13 Regeneron Pharmaceuticals, Inc. CRISPR and AAV strategies for x-linked juvenile retinoschisis therapy
CN111073900B (zh) * 2019-12-09 2023-05-02 温氏食品集团股份有限公司 一种提高猪克隆胚胎发育效率的方法
CN111172274B (zh) * 2020-01-22 2021-10-15 清华大学 Synaptotagmin-7在双向情感障碍诊断和治疗中的用途
WO2021168412A1 (fr) * 2020-02-21 2021-08-26 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Modèles d'animaux non humains pour le vieillissement et/ou la neurodégénérescence
CN111235154A (zh) * 2020-04-01 2020-06-05 陕西慧康生物科技有限责任公司 靶向敲除人MC1R基因的sgRNA及其构建的细胞株
CN111235153B (zh) * 2020-04-01 2023-05-26 陕西慧康生物科技有限责任公司 靶向敲除人MC1R基因的sgRNA及其构建的细胞株
WO2022145495A1 (fr) * 2021-01-04 2022-07-07 Modalis Therapeutics Corporation Procédé de traitement d'ataxies spinocérébelleuses (sca) par ciblage du gène atxn7
IL282597B (en) * 2021-04-22 2022-08-01 B G Negev Technologies And Applications Ltd At Ben Gurion Univ Birds for producing female hatchling and methods of producing same
CA3205739A1 (fr) * 2021-05-12 2022-11-17 Eva CZIRR Procedes et compositions pour traiter des troubles associes au vieillissement avec des modulateurs de l'element 2 de la famille du facteur en trefle
CN113355356A (zh) * 2021-07-05 2021-09-07 西北农林科技大学 一种csn1s1基因敲除载体及csn1s1基因敲除乳腺上皮细胞的制备方法
CN113881646B (zh) * 2021-09-28 2023-08-04 北京市农林科学院 参与植物抗病的相关蛋白TaFAH1及其基因和应用
CN113916754B (zh) * 2021-10-12 2023-11-10 四川大学华西医院 用于检测乳腺癌患者循环肿瘤细胞的细胞表面标志物及应用
KR20240143936A (ko) * 2023-03-21 2024-10-02 주식회사 키베이직 콧물 시료를 이용한 반려동물의 인지 장애 진단용 조성물
CN118943377B (zh) * 2024-07-18 2025-05-06 江阴纳力新材料科技有限公司 一种复合集流体及应用其的极片、二次电池
CN119372338B (zh) * 2024-12-11 2025-08-01 广东省农业科学院动物科学研究所 与番鸭繁殖性状相关的ulk1基因snp分子标记及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (fr) * 2002-01-23 2003-10-23 The University Of Utah Research Foundation Mutagenese chromosomique ciblee au moyen de nucleases en doigt a zinc

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203140A1 (en) * 2007-09-27 2009-08-13 Sangamo Biosciences, Inc. Genomic editing in zebrafish using zinc finger nucleases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (fr) * 2002-01-23 2003-10-23 The University Of Utah Research Foundation Mutagenese chromosomique ciblee au moyen de nucleases en doigt a zinc

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GEURTS ARON M ET AL: "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 325, no. 5939, 24 July 2009 (2009-07-24), pages 433, XP002580718, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1172447 *
MILLER JEFFREY C ET AL: "An improved zinc-finger nuclease architecture for highly specific genome editing", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 25, no. 7, 1 July 2007 (2007-07-01), pages 778 - 785, XP002465119, ISSN: 1087-0156, DOI: 10.1038/NBT1319 *
PORTEUS M H ET AL: "GENE TARGETING USING ZINC FINGER NUCLEASES", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 23, no. 8, 1 August 2005 (2005-08-01), pages 967 - 973, XP002467422, ISSN: 1087-0156, DOI: 10.1038/NBT1125 *
See also references of WO2011011767A1 *
URNOV F D ET AL: "Highly efficient endogenous human gene correction using designed zinc-finger nucleases", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 435, no. 7042, 2 June 2005 (2005-06-02), pages 646 - 651, XP002411069, ISSN: 0028-0836, DOI: 10.1038/NATURE03556 *

Also Published As

Publication number Publication date
EP2456877A1 (fr) 2012-05-30
IL217409A0 (en) 2012-02-29
CA2767377A1 (fr) 2011-01-27
WO2011011767A1 (fr) 2011-01-27
JP2013500018A (ja) 2013-01-07
SG177711A1 (en) 2012-02-28
KR20120097483A (ko) 2012-09-04
AU2010275432A1 (en) 2012-02-02

Similar Documents

Publication Publication Date Title
EP2456877A4 (fr) Procédé d'édition de génome
EP2266067A4 (fr) Procédé de génotypage de patient
BR112012004593A2 (pt) "método"
EP2422916A4 (fr) Procédé d'usinage laser
EP2231257A4 (fr) Procédé d'application
EP2423323A4 (fr) Procédé d'évaluation du cancer
EP2401048A4 (fr) Procédé de séparation
BR112012000821A2 (pt) "processo"
BR112012022750A2 (pt) "método de produção de radionuclídeos"
EP2508653A4 (fr) Procédé de fabrication d'un monocristal
EP2650821A4 (fr) Procédé de rognure d'image de texte
EP2128151A4 (fr) Procédé de purification d'ester cyclique
EP2391391A4 (fr) Procédé de stérilisation
EP2508430A4 (fr) Procédé d'élimination de débris spatiaux
EP2396281A4 (fr) Procédé de désioduration
EP2450303A4 (fr) Procédé de renouvellement d'ascenseur
EP2152945A4 (fr) Procédé de décortication
EP2495227A4 (fr) Procédé de fabrication d'oléfines
EP2516643A4 (fr) Procédé de mutagenèse
EP2405946A4 (fr) Procédé de stérilisation
EP2524337A4 (fr) Procédé de criblage de mode d'action
EP2308819A4 (fr) Procédé de synthèse de sévoflurane
EP2531626A4 (fr) Procédé d'extraction
EP2143508A4 (fr) Procédé d'hydroformage
EP2210169A4 (fr) Procédé de saisie de caractères

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20120208

A4 Supplementary search report drawn up and despatched

Effective date: 20120412

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
17Q First examination report despatched

Effective date: 20140205

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20150730