EP4598511A1 - Procédé de fabrication d'une substance cible anthropique - Google Patents

Procédé de fabrication d'une substance cible anthropique

Info

Publication number
EP4598511A1
EP4598511A1 EP23776273.7A EP23776273A EP4598511A1 EP 4598511 A1 EP4598511 A1 EP 4598511A1 EP 23776273 A EP23776273 A EP 23776273A EP 4598511 A1 EP4598511 A1 EP 4598511A1
Authority
EP
European Patent Office
Prior art keywords
superabsorbent
liquid
target substance
liquid volume
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP23776273.7A
Other languages
German (de)
English (en)
Inventor
Timo Hillebrand
Elmara Graser
Wiebke JACOBI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IST Innuscreen GmbH
Original Assignee
IST Innuscreen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IST Innuscreen GmbH filed Critical IST Innuscreen GmbH
Publication of EP4598511A1 publication Critical patent/EP4598511A1/fr
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Definitions

  • the invention relates to a process for producing an anthropogenic target substance.
  • Superabsorbent polymers are plastics that are able to absorb many times their own weight in polar liquids. These are mainly water or aqueous solutions. When the liquid is absorbed, the superabsorbent swells and forms a hydrogel. Hydrogels can form all cross-linked polymers that are polar (e.g. polyacrylamide, polyvinylpyrrolidone, amylopectin, gelatin, cellulose).
  • acrylamide sodium salt of acrylic acid
  • acrylamide acrylamide
  • a so-called core cross-linker (CXL) is added to the monomer solution, which connects the long-chain polymer molecules formed to one another in places using chemical bridges, also known as cross-linking. These bridges make the polymer insoluble in water.
  • This so-called base polymer may be subjected to a process known as surface cross-linking (SXL). This involves applying another chemical to the surface of the particles, which, when heated, creates a second network only on the outer layer of the grain. This shell supports the swollen gel so that it stays together even under external stress (movement, pressure).
  • the product is traditionally used, for example, as white granules with particle sizes of 100 to 1000 pm. It is mainly used in baby diapers, sanitary napkins, incontinence care, in dressing material and, in small quantities, in cable sheathing for deep-sea cables.
  • Other areas of application include so-called gel beds, gel-forming extinguishing agents in fire fighting, as a mechanical stabilizer for cut flowers in a vase or as an additive for plant soil to permanently store water.
  • acrylic acid neutralized with potassium hydroxide is used because of its better environmental compatibility.
  • the use of superabsorbents is known as toys under names such as "water beads", "aqua beads" or "water beads”.
  • the nanoparticles are present in a high concentration in the second liquid volume.
  • the concentration or the presence of the correct substance can be examined in a subsequent analysis, e.g. by means of a spectroscopic examination.
  • the second liquid volume can also be further concentrated in a cascading process in one or more additional stages, e.g. by adding a superabsorbent again or adding it to a superabsorbent again and incubating again, or by using a conventional method for concentrating target substances, e.g. by one of the methods given at the beginning. If only part of the liquid portion is removed as a second liquid volume, the target substance in the liquid portion of the mixture remaining after removal can be further concentrated by incubating again over a second period of time. Both variants of the process can be repeated several times so that a higher concentration of the target substance is obtained at each stage of the cascaded concentration.
  • the further concentration of the target substance in the withdrawn second liquid volume can be carried out by means of a filtration, ultrafiltration or precipitation reaction technique.
  • the further concentration of the target substance in the second liquid volume taken can also be carried out again - and optionally repeated in cascading fashion once or several times - by carrying out the following process steps:
  • Fig. 1 is a schematic representation of the cascading concentration of a target substance in a liquid: a) liquid before adding a superabsorbent; b) liquid after adding a superabsorbent and incubating the mixture; c) Second volume of liquid removed from the mixture after adding another superabsorbent and incubating the mixture; d) Remaining mixture of liquid and superabsorbent, if applicable after removing the second volume of liquid and after incubating again;
  • Further processing can, for example, be a quantitative and/or qualitative detection of the target substance in the liquid.
  • the detection is carried out using a spectroscopic or microscopic method, for example.
  • the degree of concentration and the speed of this process can be controlled very precisely by the type of superabsorbent used, by the amount used, or by the incubation time and/or the incubation temperature.
  • the process can therefore easily solve the problem of concentration when producing nanoparticles.
  • the process shown in Fig. 1 a and b does not require equipment such as ultracentrifuges, expensive ultrafiltration membranes, complex processes such as PEG precipitation or general precipitation reactions for concentrating nucleic acids, etc.
  • the process is flexible in terms of the type of nanoparticles. universally applicable.
  • Another advantage is that the superabsorbents are non-toxic and harmless and often biodegradable. The method according to the invention can therefore greatly simplify the investigation of low-concentration nanoparticles.
  • a cascading concentration of the target substance is possible.
  • the described method with the above-mentioned steps 1-3 can be used to concentrate the target substance.
  • the second liquid volume 4 can be reduced in volume to re-concentrate the target substance. This can be done either by means of a conventional filtration or precipitation process or other conventional methods. Alternatively, the re-concentration of the target substance in the second liquid volume 4 can also be carried out, as shown in Fig.
  • the liquid portion 6 remaining in the mixture with the superabsorbent 2 after removal of the second liquid volume 4 can be further reduced by incubating the mixture over a third period of time t3, as shown in Fig. 1 d.
  • the latex nanoparticles were provided by the Fraunhofer Institute for Applied Polymer Research.
  • the concentration of the latex particles in the stock solution was 2.02 M%.
  • the particles were added to a 500 ml water sample, thereby producing a 1:10,000-fold dilution.
  • the industrial process includes, for example, a sol-gel process, an emulsion polymerization process, or an interfacial polymerization process.
  • the nanoparticles or nano-particles have an average size of less than one micrometer.
  • the suspension can also be produced by crystallization or complex formation. Adding the emulsion or suspension to the superabsorbent results in the superabsorbent absorbing the liquid of the emulsion or suspension, so that the nanoparticles or nanoparticles are present in a high concentration in the remaining residue.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Colloid Chemistry (AREA)

Abstract

L'invention concerne un procédé de fabrication d'une substance cible anthropique, lequel comprend les étapes suivantes : produire une émulsion ou une suspension de la substance cible, l'émulsion ou la suspension contenant des particules et/ou des fragments de la substance cible ayant une taille moyenne de particules ou de fragments de l'ordre du nanomètre, et concentrer l'émulsion ou la suspension, lesdites étapes intervenant de la manière suivante : a) ajouter un superabsorbant (2) à un premier volume initial de liquide (1) de l'émulsion ou de la suspension ou ajouter le premier volume de liquide (1) au superabsorbant (2), b) faire incuber le mélange composé du superabsorbant (2) et du premier volume de liquide (1) pendant une première période (t1), et prélever un second volume de liquide (4) de la fraction liquide (3) du mélange, obtenue après incubation.
EP23776273.7A 2022-10-06 2023-09-13 Procédé de fabrication d'une substance cible anthropique Pending EP4598511A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102022125807.6A DE102022125807A1 (de) 2022-10-06 2022-10-06 Verfahren zur Herstellung einer anthropogenen Zielsubstanz
PCT/EP2023/075134 WO2024074274A1 (fr) 2022-10-06 2023-09-13 Procédé de fabrication d'une substance cible anthropique

Publications (1)

Publication Number Publication Date
EP4598511A1 true EP4598511A1 (fr) 2025-08-13

Family

ID=88188778

Family Applications (1)

Application Number Title Priority Date Filing Date
EP23776273.7A Pending EP4598511A1 (fr) 2022-10-06 2023-09-13 Procédé de fabrication d'une substance cible anthropique

Country Status (4)

Country Link
EP (1) EP4598511A1 (fr)
CN (1) CN119907660A (fr)
DE (1) DE102022125807A1 (fr)
WO (1) WO2024074274A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058453A1 (fr) * 2003-12-15 2005-06-30 Preentec Ag Procede de concentration et de purification de composes biologiques
DE102008023297B4 (de) 2008-05-08 2014-06-26 Aj Innuscreen Gmbh Verfahren zur Anreicherung und Isolierung von Nukleinsäuren oder Viren
US20130231460A1 (en) * 2010-09-08 2013-09-05 Qiagen Gmbh Method and device for concentrating target compounds
DE102017220514A1 (de) * 2017-11-16 2019-05-16 Unisensor Sensorsysteme Gmbh Verfahren und Vorrichtung zur Detektion von Fremdstoffen in einem flüssigen Medium
CN111771121A (zh) * 2018-02-26 2020-10-13 巴斯夫欧洲公司 测量超吸收剂渗透性的方法
JP7482911B2 (ja) * 2019-07-04 2024-05-14 ベーアーエスエフ・エスエー 超吸収体の特性を決定する方法
EP4437098A1 (fr) * 2021-11-22 2024-10-02 IST Innuscreen GmbH Procédé de concentration d'au moins une substance cible dans un échantillon liquide
WO2023089078A1 (fr) * 2021-11-22 2023-05-25 Ist Innuscreen Gmbh Procédé de concentration en cascade d'au moins une substance cible dans un échantillon liquide
DE102021134613A1 (de) * 2021-12-23 2023-06-29 Ist Innuscreen Gmbh Verfahren zum Aufkonzentrieren mindestens einer biologischen Zielsubstanz in einer Probeflüssigkeit und Verfahren zur automatisierten Online-Detektion mindestens einer biologischen Zielsubstanz in einer Probeflüssigkeit

Also Published As

Publication number Publication date
WO2024074274A1 (fr) 2024-04-11
CN119907660A (zh) 2025-04-29
DE102022125807A1 (de) 2024-04-11

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