EP4735636A1 - Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués - Google Patents

Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués

Info

Publication number
EP4735636A1
EP4735636A1 EP24743576.1A EP24743576A EP4735636A1 EP 4735636 A1 EP4735636 A1 EP 4735636A1 EP 24743576 A EP24743576 A EP 24743576A EP 4735636 A1 EP4735636 A1 EP 4735636A1
Authority
EP
European Patent Office
Prior art keywords
nucleotide
intensity
wavelength
labeled
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24743576.1A
Other languages
German (de)
English (en)
Inventor
Abde Ali Hunaid Kagalwalla
John Silvio Vieceli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4735636A1 publication Critical patent/EP4735636A1/fr
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne également des systèmes et des procédés de séquençage d'ADN. Des systèmes et des procédés pour déterminer la séquence nucléotidique d'un polynucléotide peuvent comprendre l'introduction d'un quatrième nucléotide marqué dans un séquençage à deux canaux par un système de synthèse qui permet le codage de l'espace pour une détection d'un cinquième nucléotide marqué ou d'une détection de puits vide.
EP24743576.1A 2023-06-30 2024-06-25 Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués Pending EP4735636A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363511364P 2023-06-30 2023-06-30
PCT/US2024/035411 WO2025006466A1 (fr) 2023-06-30 2024-06-25 Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués

Publications (1)

Publication Number Publication Date
EP4735636A1 true EP4735636A1 (fr) 2026-05-06

Family

ID=91953762

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24743576.1A Pending EP4735636A1 (fr) 2023-06-30 2024-06-25 Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués

Country Status (2)

Country Link
EP (1) EP4735636A1 (fr)
WO (1) WO2025006466A1 (fr)

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3924454A1 (de) 1989-07-24 1991-02-07 Cornelis P Prof Dr Hollenberg Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips)
DE69126530T2 (de) 1990-07-27 1998-02-05 Isis Pharmaceutical, Inc., Carlsbad, Calif. Nuklease resistente, pyrimidin modifizierte oligonukleotide, die die gen-expression detektieren und modulieren
IT1242565B (it) 1990-09-14 1994-05-16 Marposs Spa Apparecchiatura per il controllo di una pluralita' di pezzi meccanici e relativo metodo di controllo.
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
EP1256589A3 (fr) 1991-11-26 2003-09-17 Isis Pharmaceuticals, Inc. Oligomères contenant des pyrimidines modifiées
AU3728093A (en) 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1994022892A1 (fr) 1993-03-30 1994-10-13 Sterling Winthrop Inc. Oligonucleotides modifies contant des nucleosides 7-deazapurines
EP0695306A1 (fr) 1993-04-19 1996-02-07 Gilead Sciences, Inc. Formation a helice triple et double a l'aide d'oligomeres contenant des purines modifiees
US5329807A (en) 1993-06-18 1994-07-19 W. L. Gore & Associates, Inc. Centrifuge test apparatus for footwear and apparel
US5472672A (en) 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
DE69433180T2 (de) 1993-10-26 2004-06-24 Affymetrix, Inc., Santa Clara Felder von nukleinsaeuresonden auf biologischen chips
EP0742987A4 (fr) 1994-01-10 1998-01-07 Richard C Fuisz Visualisation d'objects mis en images a partir de points de vue selectionnes
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
US6382591B1 (en) 1995-02-16 2002-05-07 Flowserve Management Company Plug valve assembly
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
WO1997014028A2 (fr) 1995-10-11 1997-04-17 Luminex Corporation Procedes et appareil d'analyse multiplexee de specimens cliniques
US5658734A (en) 1995-10-17 1997-08-19 International Business Machines Corporation Process for synthesizing chemical compounds
US6105331A (en) 1996-03-29 2000-08-22 Braas Gmbh Joist element for fastening a flat, plate-shaped structural element to a pitched roof
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5874919A (en) 1997-01-09 1999-02-23 Harris Corporation Stub-tuned, proximity-fed, stacked patch antenna
EP1591541B1 (fr) 1997-04-01 2012-02-15 Illumina Cambridge Limited Methode de séquençage d'acide nucléique
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6104631A (en) 1997-12-17 2000-08-15 National Scientific Corp. Static memory cell with load circuit using a tunnel diode
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US6610382B1 (en) 1998-10-05 2003-08-26 3M Innovative Properties Company Friction control article for wet and dry applications
US6316949B1 (en) 1999-01-19 2001-11-13 Nidec-Read Corporation Apparatus and method for testing electric conductivity of circuit path ways on circuit board
CA2370976C (fr) 1999-04-20 2009-10-20 Illumina, Inc. Detection de reactions d'acide nucleique sur microsupports de billes en reseau
JP3403971B2 (ja) 1999-06-02 2003-05-06 富士通株式会社 パケット転送装置
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
JP2001223726A (ja) 2000-02-10 2001-08-17 Toyo Microsystems Corp 多重通信方法、多重通信装置および多重通信システム
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US6988920B2 (en) 2002-01-09 2006-01-24 Tzong In Yeh Slider having improved resistance to erosion and wear
JP2003289195A (ja) 2002-03-28 2003-10-10 Mitsubishi Electric Corp 冷却装置
EP2607369B1 (fr) 2002-08-23 2015-09-23 Illumina Cambridge Limited Nucléotides modifiés pour le séquençage de polynucléotide
US6991893B2 (en) 2002-10-31 2006-01-31 Intel Corporation Controlling resist profiles through substrate modification
US6836969B1 (en) 2003-06-18 2005-01-04 Randy N. Bates Sliding gauge for making measured markings
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
EP1828412B2 (fr) 2004-12-13 2019-01-09 Illumina Cambridge Limited Procede ameliore de detection de nucleotides
US8623628B2 (en) 2005-05-10 2014-01-07 Illumina, Inc. Polymerases
CA2615323A1 (fr) 2005-06-06 2007-12-21 454 Life Sciences Corporation Sequencage d'extremites appariees
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
GB0718255D0 (en) 2007-09-19 2007-10-31 Univ Edinburgh Nucleobase characterisation
US8617811B2 (en) * 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CA3138752C (fr) 2012-04-03 2024-02-06 Illumina, Inc. Tete de lecture optoelectronique integree et cartouche fluidique utile pour le sequencage d'acide nucleique
ES2833524T3 (es) * 2012-05-02 2021-06-15 Ibis Biosciences Inc Secuenciación de ADN
EP3438286B1 (fr) * 2012-05-02 2020-10-28 Ibis Biosciences, Inc. Séquençage d'adn
RU2736384C1 (ru) 2017-03-07 2020-11-16 Иллюмина, Инк. Секвенирование с помощью одиночного источника света и двух оптических каналов
CN112840035B (zh) * 2018-11-07 2024-01-30 青岛华大智造科技有限责任公司 对多核苷酸进行测序的方法

Also Published As

Publication number Publication date
WO2025006466A1 (fr) 2025-01-02

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