EP4735636A1 - Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués - Google Patents
Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marquésInfo
- Publication number
- EP4735636A1 EP4735636A1 EP24743576.1A EP24743576A EP4735636A1 EP 4735636 A1 EP4735636 A1 EP 4735636A1 EP 24743576 A EP24743576 A EP 24743576A EP 4735636 A1 EP4735636 A1 EP 4735636A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleotide
- intensity
- wavelength
- labeled
- sequencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne également des systèmes et des procédés de séquençage d'ADN. Des systèmes et des procédés pour déterminer la séquence nucléotidique d'un polynucléotide peuvent comprendre l'introduction d'un quatrième nucléotide marqué dans un séquençage à deux canaux par un système de synthèse qui permet le codage de l'espace pour une détection d'un cinquième nucléotide marqué ou d'une détection de puits vide.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202363511364P | 2023-06-30 | 2023-06-30 | |
| PCT/US2024/035411 WO2025006466A1 (fr) | 2023-06-30 | 2024-06-25 | Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP4735636A1 true EP4735636A1 (fr) | 2026-05-06 |
Family
ID=91953762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP24743576.1A Pending EP4735636A1 (fr) | 2023-06-30 | 2024-06-25 | Systèmes et procédés de séquençage de polynucléotides avec quatre nucléotides marqués |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP4735636A1 (fr) |
| WO (1) | WO2025006466A1 (fr) |
Family Cites Families (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3924454A1 (de) | 1989-07-24 | 1991-02-07 | Cornelis P Prof Dr Hollenberg | Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips) |
| DE69126530T2 (de) | 1990-07-27 | 1998-02-05 | Isis Pharmaceutical, Inc., Carlsbad, Calif. | Nuklease resistente, pyrimidin modifizierte oligonukleotide, die die gen-expression detektieren und modulieren |
| IT1242565B (it) | 1990-09-14 | 1994-05-16 | Marposs Spa | Apparecchiatura per il controllo di una pluralita' di pezzi meccanici e relativo metodo di controllo. |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| EP1256589A3 (fr) | 1991-11-26 | 2003-09-17 | Isis Pharmaceuticals, Inc. | Oligomères contenant des pyrimidines modifiées |
| AU3728093A (en) | 1992-02-19 | 1993-09-13 | Public Health Research Institute Of The City Of New York, Inc., The | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids |
| WO1994022892A1 (fr) | 1993-03-30 | 1994-10-13 | Sterling Winthrop Inc. | Oligonucleotides modifies contant des nucleosides 7-deazapurines |
| EP0695306A1 (fr) | 1993-04-19 | 1996-02-07 | Gilead Sciences, Inc. | Formation a helice triple et double a l'aide d'oligomeres contenant des purines modifiees |
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| US5472672A (en) | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
| DE69433180T2 (de) | 1993-10-26 | 2004-06-24 | Affymetrix, Inc., Santa Clara | Felder von nukleinsaeuresonden auf biologischen chips |
| EP0742987A4 (fr) | 1994-01-10 | 1998-01-07 | Richard C Fuisz | Visualisation d'objects mis en images a partir de points de vue selectionnes |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US6150510A (en) | 1995-11-06 | 2000-11-21 | Aventis Pharma Deutschland Gmbh | Modified oligonucleotides, their preparation and their use |
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| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5624711A (en) | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
| WO1997014028A2 (fr) | 1995-10-11 | 1997-04-17 | Luminex Corporation | Procedes et appareil d'analyse multiplexee de specimens cliniques |
| US5658734A (en) | 1995-10-17 | 1997-08-19 | International Business Machines Corporation | Process for synthesizing chemical compounds |
| US6105331A (en) | 1996-03-29 | 2000-08-22 | Braas Gmbh | Joist element for fastening a flat, plate-shaped structural element to a pitched roof |
| US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| US5874919A (en) | 1997-01-09 | 1999-02-23 | Harris Corporation | Stub-tuned, proximity-fed, stacked patch antenna |
| EP1591541B1 (fr) | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Methode de séquençage d'acide nucléique |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US6104631A (en) | 1997-12-17 | 2000-08-15 | National Scientific Corp. | Static memory cell with load circuit using a tunnel diode |
| US6277628B1 (en) | 1998-10-02 | 2001-08-21 | Incyte Genomics, Inc. | Linear microarrays |
| US6610382B1 (en) | 1998-10-05 | 2003-08-26 | 3M Innovative Properties Company | Friction control article for wet and dry applications |
| US6316949B1 (en) | 1999-01-19 | 2001-11-13 | Nidec-Read Corporation | Apparatus and method for testing electric conductivity of circuit path ways on circuit board |
| CA2370976C (fr) | 1999-04-20 | 2009-10-20 | Illumina, Inc. | Detection de reactions d'acide nucleique sur microsupports de billes en reseau |
| JP3403971B2 (ja) | 1999-06-02 | 2003-05-06 | 富士通株式会社 | パケット転送装置 |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| JP2001223726A (ja) | 2000-02-10 | 2001-08-17 | Toyo Microsystems Corp | 多重通信方法、多重通信装置および多重通信システム |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US6988920B2 (en) | 2002-01-09 | 2006-01-24 | Tzong In Yeh | Slider having improved resistance to erosion and wear |
| JP2003289195A (ja) | 2002-03-28 | 2003-10-10 | Mitsubishi Electric Corp | 冷却装置 |
| EP2607369B1 (fr) | 2002-08-23 | 2015-09-23 | Illumina Cambridge Limited | Nucléotides modifiés pour le séquençage de polynucléotide |
| US6991893B2 (en) | 2002-10-31 | 2006-01-31 | Intel Corporation | Controlling resist profiles through substrate modification |
| US6836969B1 (en) | 2003-06-18 | 2005-01-04 | Randy N. Bates | Sliding gauge for making measured markings |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US20110059865A1 (en) | 2004-01-07 | 2011-03-10 | Mark Edward Brennan Smith | Modified Molecular Arrays |
| EP1828412B2 (fr) | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Procede ameliore de detection de nucleotides |
| US8623628B2 (en) | 2005-05-10 | 2014-01-07 | Illumina, Inc. | Polymerases |
| CA2615323A1 (fr) | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Sequencage d'extremites appariees |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
| GB0718255D0 (en) | 2007-09-19 | 2007-10-31 | Univ Edinburgh | Nucleobase characterisation |
| US8617811B2 (en) * | 2008-01-28 | 2013-12-31 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| CA3138752C (fr) | 2012-04-03 | 2024-02-06 | Illumina, Inc. | Tete de lecture optoelectronique integree et cartouche fluidique utile pour le sequencage d'acide nucleique |
| ES2833524T3 (es) * | 2012-05-02 | 2021-06-15 | Ibis Biosciences Inc | Secuenciación de ADN |
| EP3438286B1 (fr) * | 2012-05-02 | 2020-10-28 | Ibis Biosciences, Inc. | Séquençage d'adn |
| RU2736384C1 (ru) | 2017-03-07 | 2020-11-16 | Иллюмина, Инк. | Секвенирование с помощью одиночного источника света и двух оптических каналов |
| CN112840035B (zh) * | 2018-11-07 | 2024-01-30 | 青岛华大智造科技有限责任公司 | 对多核苷酸进行测序的方法 |
-
2024
- 2024-06-25 EP EP24743576.1A patent/EP4735636A1/fr active Pending
- 2024-06-25 WO PCT/US2024/035411 patent/WO2025006466A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2025006466A1 (fr) | 2025-01-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
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|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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| 17P | Request for examination filed |
Effective date: 20251211 |
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| AK | Designated contracting states |
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