ES2003314T3 - Enzimas amiloliticas que producen microorganismos construidos por tecnologia adn recombinante y su uso para procedimientos de fermentacion. - Google Patents

Enzimas amiloliticas que producen microorganismos construidos por tecnologia adn recombinante y su uso para procedimientos de fermentacion.

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Publication number
ES2003314T3
ES2003314T3 ES87110370T ES87110370T ES2003314T3 ES 2003314 T3 ES2003314 T3 ES 2003314T3 ES 87110370 T ES87110370 T ES 87110370T ES 87110370 T ES87110370 T ES 87110370T ES 2003314 T3 ES2003314 T3 ES 2003314T3
Authority
ES
Spain
Prior art keywords
enzymes
amylolytic enzymes
recombinant dna
dna technology
amilolytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87110370T
Other languages
English (en)
Other versions
ES2003314A4 (es
Inventor
Alexander Strasser
Feodor Bernard Martens
Jurgen Dohmen
Cornelius P Hollenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES2003314A4 publication Critical patent/ES2003314A4/es
Application granted granted Critical
Publication of ES2003314T3 publication Critical patent/ES2003314T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242Fungal source
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/047Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00Processes specially adapted for making special kinds of beer
    • C12C12/002Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/004Genetically modified microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00Processes specially adapted for making special kinds of beer
    • C12C12/002Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/006Yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00Processes specially adapted for making special kinds of beer
    • C12C12/02Beer with low calorie content
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

ESTA INVENCION PRESENTA UN METODO PARA LA PRODUCCION DE ENZIMAS AMILOLITICAS A BASE DE CULTIVAR UN MICROORGANISMO, QUE HAN RECIBIDO COMO RESULTADO DE UNA TECNOLOGIA DEL ADN RECOMBINANTE SECUENCIAS DE ADN DESDE UNA LEVADURA DONADORA QUE CONTIENE LAS SECUENCIAS DE CODIFICACION DE LAS ENZIMAS AMILOLITICAS EN DONDE EL MICROORGANISMO HUESPED ES CAPAZ DE EXPRESAR DICHAS ENZIMAS AMILOLITICAS. ADEMAS, ESTA INVENCION PRESENTA MICROORGANISMOS QUE HAN PASADO POR TECNICAS DE INGENIERIA GENETICA DE TAL MODO QUE SON CAPACES DE PRODUCIR Y EXPRESAR LAS ENZIMAS AMILOLITICAS, UN VECTOR QUE CONTIENE LAS SECUENCIAS DE ADN, QUE CODIFICA LAS ENZIMAS AMILOLITICAS Y LAS SECUENCIAS DE ADN RESPECTIVAS. LOS MICROORGANISMOS HUESPEDES ANTEDICHOS SON UTILES PARA LA PRODUCCION DE BIOMASAS Y EN MUCHOS PROCESOS DE FERMENTACION, PREFERENTEMENTE EN LA PRODUCCION DE CERVEZAS ESPECIALES.
ES87110370T 1986-08-21 1987-07-17 Enzimas amiloliticas que producen microorganismos construidos por tecnologia adn recombinante y su uso para procedimientos de fermentacion. Expired - Lifetime ES2003314T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP86111586A EP0257115A1 (en) 1986-08-21 1986-08-21 Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes

Publications (2)

Publication Number Publication Date
ES2003314A4 ES2003314A4 (es) 1988-11-01
ES2003314T3 true ES2003314T3 (es) 1996-10-01

Family

ID=8195356

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87110370T Expired - Lifetime ES2003314T3 (es) 1986-08-21 1987-07-17 Enzimas amiloliticas que producen microorganismos construidos por tecnologia adn recombinante y su uso para procedimientos de fermentacion.

Country Status (7)

Country Link
US (1) US5100794A (es)
EP (1) EP0257115A1 (es)
AT (1) ATE123304T1 (es)
DE (2) DE260404T1 (es)
ES (1) ES2003314T3 (es)
GR (1) GR880300088T1 (es)
ZA (1) ZA876134B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2613726B2 (fr) * 1986-06-10 1990-07-20 Transgene Sa Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger
FR2613727B2 (fr) * 1986-06-10 1990-07-20 Transgene Sa Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis
AU3434789A (en) * 1988-03-31 1989-10-16 Upjohn Company, The The gene encoding phosphoribosylaminoimidazolecarboxylase of schwanniomyces occidentalis
ATE144281T1 (de) * 1989-04-28 1996-11-15 Rhein Biotech Proz & Prod Gmbh Hefezellen der gattung-schwanniomyces
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
US6551798B2 (en) 2000-08-04 2003-04-22 Battelle Memorial Institute Method for using a yeast alpha-amylase promoter
US6900305B2 (en) 2000-08-04 2005-05-31 Battelle Memorial Institute Isolated yeast promoter sequence and a method of regulated heterologous expression
US6541622B1 (en) 2000-08-04 2003-04-01 Battelle Memorial Institute Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression
HUE050997T2 (hu) * 2008-05-06 2021-03-01 Archer Daniels Midland Co Keményítõ- és xilánbontó enzimek expresszálásával keményítõ és xilán alkoholos fermentációjára képes hõtûrõ Hansenula polymorpha élesztõtörzsek kifejlesztése
US20100291649A1 (en) * 2009-05-18 2010-11-18 E. I. Du Pont De Nemours And Company Control of contaminant yeast in fermentation processes
CA2832279C (en) 2011-04-05 2024-03-05 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
GB201620658D0 (en) * 2016-12-05 2017-01-18 Univ Stellenbosch Recombinant yeast and use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2608540B2 (ja) * 1982-05-19 1997-05-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ クルイベロミセス種のためのクローニング系
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
CA1203765A (en) * 1983-05-11 1986-04-29 Alexander M. Sills Schwanniomyces castellii strains and brewing process
US4794175A (en) * 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
IL76190A0 (en) * 1984-08-31 1985-12-31 Smithkline Beckman Corp Recombinant saccharomyces
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
EP0233196A4 (en) * 1985-05-21 1987-11-30 Bioteknika International YEAST EXPRESSING GLUCOAMYLASE.

Also Published As

Publication number Publication date
DE3751326D1 (de) 1995-07-06
DE3751326T2 (de) 1996-01-11
US5100794A (en) 1992-03-31
ES2003314A4 (es) 1988-11-01
EP0257115A1 (en) 1988-03-02
ATE123304T1 (de) 1995-06-15
ZA876134B (en) 1988-02-29
DE260404T1 (de) 1988-09-01
GR880300088T1 (en) 1988-10-21

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