ES2009587A6 - Un metodo para purificar un polipeptido. - Google Patents

Un metodo para purificar un polipeptido.

Info

Publication number
ES2009587A6
ES2009587A6 ES8801013A ES8801013A ES2009587A6 ES 2009587 A6 ES2009587 A6 ES 2009587A6 ES 8801013 A ES8801013 A ES 8801013A ES 8801013 A ES8801013 A ES 8801013A ES 2009587 A6 ES2009587 A6 ES 2009587A6
Authority
ES
Spain
Prior art keywords
polypeptide
protein
precipitate
separate
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8801013A
Other languages
English (en)
Inventor
Vistor Nussenzweig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New York University
Original Assignee
New York University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York University filed Critical New York University
Publication of ES2009587A6 publication Critical patent/ES2009587A6/es
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/002—Protozoa antigens
    • A61K39/015—Hemosporidia antigens, e.g. Plasmodium antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

UN METODO PARA PURIFICAR UN POLIPEPTIDO. COMPRENDE: A) SOMETER UNA PREPARACION IMPURA DEL POLIPEPTIDO CONTAMINADA CON OTRAS ESPECIES PROTEICAS A UNA TEMPERATURA DE 100GC PARA DESNATURALIZAR Y PRECIPITAR DICHAS IMPUREZAS PROTEICAS; B) SEPARAR EL PRECIPITADO; C) SEPARAR LOS CONSTITUYENTES SOLIDOS DEL SOBRENADANTE; D) REDISOLVER LOS CONSTITUYENTES SOLIDOS EN UNA SOLUCION DE UN TAMPON IONICO ACUOSO; E) SOMETER LA SOLUCION A CROMATOGRAFIA DE INTERCAMBIO IONICO Y DESPUES A CROMATOGRAFIA SOBRE TAMIZ MOLECULAR. SE OBTIENE ASI UN POLIPEPTIDO PURIFICADO QUE CONSTA ESENCIALMENTE DE UNA SECUENCIA DE AMINOACIDOS QUE CONTIENE AL MENOS DOS SECUENCIAS REPETIDAS SEGUIDAS IDENTICAS A LAS DE LA REGION EPITOPICA INMUNODOMINANTE DE LA PROTEINA CIRCUMSPOROZOITA DE P. VIVAX. ESTE POLIPEPTIDO TIENE UTILIDAD EN UNA VACUNA CONTRA LA MALARIA.
ES8801013A 1987-03-30 1988-03-30 Un metodo para purificar un polipeptido. Expired ES2009587A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3232687A 1987-03-30 1987-03-30

Publications (1)

Publication Number Publication Date
ES2009587A6 true ES2009587A6 (es) 1989-10-01

Family

ID=21864341

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8801013A Expired ES2009587A6 (es) 1987-03-30 1988-03-30 Un metodo para purificar un polipeptido.

Country Status (9)

Country Link
EP (1) EP0309555A4 (es)
JP (1) JPH01503514A (es)
AU (1) AU617668B2 (es)
ES (1) ES2009587A6 (es)
IL (1) IL85905A0 (es)
NZ (1) NZ224086A (es)
PT (2) PT87137B (es)
WO (1) WO1988007546A1 (es)
ZA (1) ZA882272B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4826957A (en) * 1985-07-12 1989-05-02 New York University Immunogenic recombinant yeast expression product and method for purifying it
AU625713B2 (en) * 1988-02-19 1992-07-16 Microgenesys, Inc. Method for producing recombinant protein derived from the circumsporozoite gene of plasmodium falciparum
SE8904100D0 (sv) * 1989-12-05 1989-12-05 Astra Ab New analysis method
NZ233255A (en) * 1989-04-11 1993-02-25 Chiron Corp Plasmodium cs protein analogues lacking one or more of the repeat epitopes, and containing at least one nonrepeat flanking epitope
US5095093A (en) * 1990-11-06 1992-03-10 The United States Of America As Represented By The Secretary Of The Navy Protective four amino acid epitope against Plasmodium vivax malaria
WO1993011157A1 (en) * 1991-11-27 1993-06-10 The Council Of The Queensland Institute Of Medical Research MALARIAL VACCINE AND PEPTIDES COMPRISING HUMAN T-CELL EPITOPE OF CIRCUMSPOROZOITE PROTEIN OF $i(P.VIVAX)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4466917A (en) * 1981-02-12 1984-08-21 New York University Malaria vaccine
US4707357A (en) * 1984-06-26 1987-11-17 The United States Of America As Represented By The Secretary Of The Army Immunologically active peptides capable of inducing immunization against malaria and genes encoding therefor
ZA856655B (en) * 1984-09-12 1986-04-30 Univ New York Cross-reactive and protective epitopes of circumsporozoite proteins
AU590599B2 (en) * 1985-07-12 1989-11-09 New York University Immunogenic peptide antigen corresponding to plasmodium vivax circumsporozoite protein
US4826957A (en) * 1985-07-12 1989-05-02 New York University Immunogenic recombinant yeast expression product and method for purifying it
US4693994A (en) * 1985-11-19 1987-09-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Protective synthetic peptide against malaria and encoding gene
EP0252588A3 (en) * 1986-05-12 1989-07-12 Smithkline Beecham Corporation Process for the isolation and purification of p. falciparum cs protein expressed in recombinant e. coli, and its use as a vaccine

Also Published As

Publication number Publication date
NZ224086A (en) 1991-02-26
IL85905A0 (en) 1988-09-30
PT87332A (pt) 1988-05-01
PT87137B (pt) 1992-07-31
EP0309555A4 (en) 1990-04-10
WO1988007546A1 (en) 1988-10-06
PT87137A (pt) 1988-04-01
AU617668B2 (en) 1991-12-05
JPH01503514A (ja) 1989-11-30
AU1593688A (en) 1988-11-02
PT87332B (pt) 1992-07-31
EP0309555A1 (en) 1989-04-05
ZA882272B (en) 1988-09-22

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19991108