ES2012323T3 - Marcadores quimioluminiscentes protegidos. - Google Patents

Marcadores quimioluminiscentes protegidos.

Info

Publication number
ES2012323T3
ES2012323T3 ES89301679T ES89301679T ES2012323T3 ES 2012323 T3 ES2012323 T3 ES 2012323T3 ES 89301679 T ES89301679 T ES 89301679T ES 89301679 T ES89301679 T ES 89301679T ES 2012323 T3 ES2012323 T3 ES 2012323T3
Authority
ES
Spain
Prior art keywords
marker
protected
markers
protective
phenylic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89301679T
Other languages
English (en)
Other versions
ES2012323A4 (es
Inventor
Lyle John Arnold
Iii Alexander Atkinson Waldrop
Philip W Hammond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of ES2012323A4 publication Critical patent/ES2012323A4/es
Application granted granted Critical
Publication of ES2012323T3 publication Critical patent/ES2012323T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Details Of Garments (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Quinoline Compounds (AREA)
  • Other In-Based Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN METODO PARA PREPARAR UNA PAREJA ENLAZANTE ESPECIFICA MARCADA, TAL COMO UNA SONDA ESPECIFICA, EN FORMA DE UNA SONDA DE ANTICUERPO U OLIGONUCLEOTIDO UTILIZANDO UN MARCADOR PROTEGIDO, SIENDO SUSCEPTIBLE DE INACTIVACION EL MARCADOR NO PROTEGIDO CORRESPONDIENTE, TAL COMO POR HIDROLISIS, PARA PRODUCIR UNA FORMA NO QUIMILUMINISCENTE DEL MARCADOR. LA PAREJA ENLAZANTE ESPECIFICA SE UNE AL MARCADOR Y SE PREPARA UN ADUCTO DEL MARCADOR UTILIZANDO UN PRIMER ADUCTO PROTECTOR, QUE PRODUCE UN MARCADOR PROTEGIDO, QUE ES MENOS SUSCEPTIBLE A LA INACTIVACION. MARCADORES ESPECIALMENTE PREFERIDOS SON LOS ACRIDINIOS Y ACRIDANOS, MAS PREFERIBLEMENTE LOS PRIMEROS, QUE TIENEN LA ESTRUCTURA GENERAL: (FIG. A), DONDE LOS ANILLOS FENILICOS ESTAN SUSTITUIDOS ADICIONALMENTE DE MODO OPCIONAL, R1 ES PREFERIBLEMENTE UN ALQUILO, Y R5 ES OPCIONALMENTE UN HIDROCARBURO SUSTITUIDO, MAS PREFERIBLEMENTE UNA PARTE FENILICA QUE, O BIEN ESTA UNIDA A LA PAREJA ENLAZANTE ESPECIFICA, O ES CAPAZ DE ESTAR UNIDA A ELLA. LA FORMACION DELMARCADOR PROTEGIDO ES PREFERIBLEMENTE UNA REACCION EN EQUILIBRIO, QUE ES FACILMENTE REVERSIBLE, TAL COMO POR DILUCION U OXIDACION DEL PRIMER ADUCTO PROTECTOR. TAMBIEN SE DESCRIBEN MARCADORES UTILES EN TAL METODO.
ES89301679T 1988-02-26 1989-02-22 Marcadores quimioluminiscentes protegidos. Expired - Lifetime ES2012323T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/160,611 US4950613A (en) 1988-02-26 1988-02-26 Proteted chemiluminescent labels

Publications (2)

Publication Number Publication Date
ES2012323A4 ES2012323A4 (es) 1990-03-16
ES2012323T3 true ES2012323T3 (es) 1996-06-16

Family

ID=22577607

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89301679T Expired - Lifetime ES2012323T3 (es) 1988-02-26 1989-02-22 Marcadores quimioluminiscentes protegidos.

Country Status (15)

Country Link
US (1) US4950613A (es)
EP (1) EP0330433B1 (es)
JP (1) JPH02503268A (es)
KR (1) KR0132290B1 (es)
AT (1) ATE137021T1 (es)
AU (1) AU626319B2 (es)
CA (1) CA1333396C (es)
DE (2) DE330433T1 (es)
DK (1) DK530489A (es)
ES (1) ES2012323T3 (es)
FI (1) FI895062A0 (es)
GR (1) GR900300054T1 (es)
NO (1) NO894218L (es)
PT (1) PT89834B (es)
WO (1) WO1989008256A1 (es)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6414152B1 (en) * 1981-12-11 2002-07-02 University Of Wales College Of Medicine Of Heath Park Luminescent labelling material and procedures
US5605795A (en) * 1986-07-24 1997-02-25 Tropix, Inc. Assays using chemiluminescent, enzymatically cleavable substituted 1,2-dioxetanes and kits therefor
US5110932A (en) * 1986-10-06 1992-05-05 Ciba Corning Diagnostics Corp. Polysubstituted aryl acridinium esters
US5321136A (en) * 1987-12-31 1994-06-14 London Diagnostics, Inc. Peri substituted fused ring chemiluminescent labels and their conjugates, and assays therefrom
US5290936A (en) * 1987-12-31 1994-03-01 London Diagnostics, Inc. Nitro substituted chemiluminescent labels and their conjugates, and assays therefrom
US5338847A (en) * 1987-12-31 1994-08-16 London Diagnostics, Inc. Hydrolytically stable chemiluminescent labels and their conjugates, and assays therefrom by adduct formation
US5227489A (en) * 1988-08-01 1993-07-13 Ciba Corning Diagnostics Corp. Stable hydrophilic acridinium esters suitable for liposome encapsulation
US5241070A (en) * 1988-09-26 1993-08-31 Ciba Corning Diagnostics Corp. Nucleophilic polysubstituted aryl acridinium esters and uses thereof
US5663074A (en) * 1988-09-26 1997-09-02 Chiron Diagnostics Corporation Nucleophilic polysubstituted aryl acridinium ester conjugates and syntheses thereof
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
WO1993020074A1 (en) * 1992-03-27 1993-10-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for acridine-9-carboxylic acids
WO1994002486A1 (en) * 1992-07-20 1994-02-03 Syntex (U.S.A.) Inc. Novel chemiluminescent compounds and methods of use
US6002000A (en) * 1992-07-20 1999-12-14 Dade Behring Marburg Gmbh Chemiluminescent compounds and methods of use
JP3584379B2 (ja) * 1993-02-04 2004-11-04 持田製薬株式会社 アクリジニウム化合物およびアクリジニウム化合物複合体
US5670644A (en) * 1993-05-17 1997-09-23 Lumigen, Inc. Acridan compounds
JPH08233820A (ja) * 1995-02-23 1996-09-13 Kdk Corp 化学発光測定法
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
PT915851E (pt) * 1996-07-16 2002-09-30 Staat Nl Verteg Door Min Welzi Esteres dibenzodihidropiridinecarboxilicos e seu uso no ensaio dos metodos quimiluminiscente
AU7487798A (en) * 1997-05-29 1998-12-30 Bio-Rad Laboratories, Inc. Chemiluminescent hemoglobin assay
ES2286750T3 (es) 1998-05-01 2007-12-01 Gen-Probe Incorporated Dispositivo para agitar el contenido liquido de un contenedor.
AU2012232988B2 (en) * 1998-05-01 2014-09-25 Gen-Probe Incorporated System and method for incubating the contents of a reaction receptacle
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6673560B1 (en) * 1998-11-25 2004-01-06 Bayer Corporation Measurement of hydride using chemiluminescent acridinium compounds and applications thereof
WO2000047777A1 (en) 1999-02-12 2000-08-17 Gen-Probe Incorporated Protection probes
US6127140A (en) * 1999-06-18 2000-10-03 Abbott Laboratories Assay for quantitative measurement of analytes in biological samples
US6723851B2 (en) 2001-10-31 2004-04-20 Quest Diagnostics Investment Incorporated Chemiluminescent compounds and use thereof
EP1506413B1 (en) * 2002-05-17 2016-07-06 Becton Dickinson and Company Automated system for isolating, amplyifying and detecting a target nucleic acid sequence
JP2006525027A (ja) * 2003-05-01 2006-11-09 ジェン−プロウブ インコーポレイテッド 分子スイッチを含むオリゴヌクレオチド
JPWO2006093150A1 (ja) * 2005-02-28 2008-08-07 エーザイ・アール・アンド・ディー・マネジメント株式会社 シグナル増幅方法
EP1930730B1 (en) 2005-03-10 2019-08-14 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP2752668A3 (en) 2010-07-23 2014-10-15 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
ES2778054T3 (es) 2011-11-07 2020-08-07 Beckman Coulter Inc Sistema y método para transportar recipientes de muestras
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
WO2013070755A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Centrifuge system and workflow
CN104040357B (zh) 2011-11-07 2016-11-23 贝克曼考尔特公司 等分器系统以及工作流
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
KR20140091032A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 수송 시스템의 자기 감쇠
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
AU6771981A (en) * 1979-12-19 1981-07-22 Electro-Nucleonics Inc. Chemical luminescence amplification substrate system for immuno chemistry
DE3279029D1 (en) * 1981-12-11 1988-10-20 Welsh Nat School Med Luminescent labelling materials and procedures
DE3628573C2 (de) * 1986-08-22 1994-10-13 Hoechst Ag Chemilumineszierende Acridinderivate, Verfahren zu deren Herstellung und ihre Verwendung in Lumineszenzimmunoassays
US4745181A (en) * 1986-10-06 1988-05-17 Ciba Corning Diagnostics Corp. Polysubstituted aryl acridinium esters
US4927769A (en) * 1987-07-08 1990-05-22 Ciba Corning Diagnostics Corp. Method for enhancement of chemiluminescence
DE3854029T2 (de) * 1987-09-21 1995-10-26 Gen Probe Inc Homogener Abschirmungstest.
NZ227506A (en) * 1987-12-31 1992-06-25 London Diagnostics Inc Specific binding assays using chemiluminescent compounds

Also Published As

Publication number Publication date
NO894218L (no) 1989-12-22
WO1989008256A1 (en) 1989-09-08
DK530489D0 (da) 1989-10-25
ES2012323A4 (es) 1990-03-16
DE68926259D1 (de) 1996-05-23
DE330433T1 (de) 1990-05-03
AU4072389A (en) 1989-09-22
CA1333396C (en) 1994-12-06
AU626319B2 (en) 1992-07-30
EP0330433B1 (en) 1996-04-17
JPH02503268A (ja) 1990-10-11
GR900300054T1 (en) 1991-07-31
PT89834B (pt) 1994-02-28
FI895062A7 (fi) 1989-10-25
DK530489A (da) 1989-12-27
EP0330433A3 (en) 1991-02-27
KR0132290B1 (ko) 1998-04-11
EP0330433A2 (en) 1989-08-30
PT89834A (pt) 1989-10-04
ATE137021T1 (de) 1996-05-15
KR900700885A (ko) 1990-08-17
NO894218D0 (no) 1989-10-24
FI895062A0 (fi) 1989-10-25
DE68926259T2 (de) 1996-09-19
US4950613A (en) 1990-08-21

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