ES2013408A6 - Un metodo para preparar por biotecnologia polipeptidos que tienen actividad de factor de crecimiento. - Google Patents

Un metodo para preparar por biotecnologia polipeptidos que tienen actividad de factor de crecimiento.

Info

Publication number
ES2013408A6
ES2013408A6 ES8901049A ES8901049A ES2013408A6 ES 2013408 A6 ES2013408 A6 ES 2013408A6 ES 8901049 A ES8901049 A ES 8901049A ES 8901049 A ES8901049 A ES 8901049A ES 2013408 A6 ES2013408 A6 ES 2013408A6
Authority
ES
Spain
Prior art keywords
transcription
dna sequence
biotechnology
polypeptides
growth factor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8901049A
Other languages
English (en)
Inventor
Joseph P Brown
Daniel R Twardzik
Hans Marquardt
Goerge J Todaro
A Gregory Bruce
Timothy M Rose
Anthony F Purchio
Shiu-Lok Hu
Wen Chang
Suo Win Liu
Thomas J Franceschini
Mohammed Shoyab
Greg Plowman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncogen LP
Original Assignee
Oncogen LP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen LP filed Critical Oncogen LP
Publication of ES2013408A6 publication Critical patent/ES2013408A6/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Endocrinology (AREA)
  • Dermatology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)

Abstract

METODO PARA PREPARAR POR BIOTECNOLOGIA POLIPEPTIDOS QUE TIENEN ACTIVIDAD DE FACTOR DE CRECIMIENTO. CONSISTE EN COMBINAR EN SERIE DESOXINUCLEOTIDOS PARA PRODUCIR UNA SECUENCIA DE ADN CODIFICADORA DE UN POLOPEPTIDO DE AL MENOS TRES DOMINIOS FIJABLE A RECEPTORES DE EGF, Y LIGAR A LA PORCION SINTETIZADA CUALQUIER SECUENCIA DE ADN REMANENTE. ASIMISMO, SE LIGA UNA REGION REGULADORA DE INICIACION DE TRANSCRIPCION/TRADUCCION AGUAS ARRIBA DE DICHA SECUENCIA DE ADN, SE LIGA AGUAS ABAJO DEL TERCER DOMINIO DEL POLIPEPTIDO UNA SECUENCIA REGULADORA DE TERMINACION DE TRANSCRIPCION/TRADUCCION, SE TRANSFORMA CON EL CASETE DE EXPRESION ASI OBTENIDO UNA CELULA HOSPEDANTE PROVINIENTE DE BACTERIAS, LEVADURAS, INSECTOS O MAMIFEROS, SE HACE CRECER DICHA CELULA EN UN MEDIO DE CULTIVO APROPIADO Y SE AISLA EL POLIPEPTIDO PRODUCIDO. ESTE ES UTIL COMO MITOGENO, ADITIVO NUTRICIO, MEDIO DE ENSAYO DE RECEPTORES DE EGF Y AGENTE DE CURACION Y CICATRIZACION DE HERIDAS.
ES8901049A 1988-03-24 1989-03-22 Un metodo para preparar por biotecnologia polipeptidos que tienen actividad de factor de crecimiento. Expired - Lifetime ES2013408A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17265388A 1988-03-24 1988-03-24

Publications (1)

Publication Number Publication Date
ES2013408A6 true ES2013408A6 (es) 1990-05-01

Family

ID=22628611

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8901049A Expired - Lifetime ES2013408A6 (es) 1988-03-24 1989-03-22 Un metodo para preparar por biotecnologia polipeptidos que tienen actividad de factor de crecimiento.

Country Status (22)

Country Link
JP (1) JPH0279981A (es)
KR (1) KR890014743A (es)
AU (2) AU3169589A (es)
BE (1) BE1004202A5 (es)
CH (1) CH681081A5 (es)
DE (1) DE3910084A1 (es)
DK (1) DK146789A (es)
ES (1) ES2013408A6 (es)
FI (1) FI891308A7 (es)
FR (1) FR2632321A1 (es)
GB (1) GB2219799B (es)
GR (1) GR1000876B (es)
HU (1) HUT50501A (es)
IE (1) IE890950L (es)
IL (1) IL89673A0 (es)
IT (1) IT1234961B (es)
LU (1) LU87489A1 (es)
NL (1) NL8900724A (es)
NO (1) NO891273D0 (es)
NZ (1) NZ228427A (es)
PT (1) PT90118B (es)
SE (1) SE8901031L (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293785A3 (en) * 1987-05-29 1990-05-02 Oncogen Limited Partnership Cloning and expression of simian transforming growth factor-ss1
EP0444961A1 (en) * 1990-03-02 1991-09-04 Bristol-Myers Squibb Company Her3: A novel EGF receptor homolog
EP0739984A1 (en) * 1995-04-26 1996-10-30 San Tumorforschungs-Gmbh Bivalent polypeptides containing at least two domains
US6852506B1 (en) 1996-04-10 2005-02-08 Human Genome Sciences, Inc. Extracellular/epidermal growth factor-like protein
AU5549296A (en) * 1996-04-10 1997-10-29 Human Genome Sciences, Inc. Extracellular/epidermal growth factor-like protein

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983004030A1 (en) * 1982-05-06 1983-11-24 Applied Molecular Genetics, Inc. The manufacture and expression of genes for urogastrone and polypeptide analogs thereof
JPS6028994A (ja) * 1983-07-08 1985-02-14 Wakunaga Seiyaku Kk 〔２１―ロイシン〕ヒトウロガストロン
WO1985002198A1 (en) * 1983-11-01 1985-05-23 Amgen Microbial expression of type i transforming growth factor, polypeptide analogs thereof and hybrid egf/tgf polypeptides
JP2554459B2 (ja) * 1984-07-02 1996-11-13 アース製薬 株式会社 β−ウロガストロン遺伝子、対応プラスミド組換体及び対応形質転換体
EP0199769A4 (en) * 1984-10-30 1989-11-29 Oncogen Novel polypeptides having growth factor activity and nucleic acid sequences encoding the polypeptides.
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
GB2210618B (en) * 1987-10-08 1991-10-16 British Bio Technology Synthetic egf gene
ES2081801T3 (es) * 1987-10-30 1996-03-16 Bristol Myers Squibb Co Plasmidos de expresion.
WO1989003886A1 (en) * 1987-10-30 1989-05-05 Oncogen, A Limited Partnership Expression systems for preparation of polypeptides in prokaryotic cells

Also Published As

Publication number Publication date
AU3169589A (en) 1989-09-28
SE8901031D0 (sv) 1989-03-22
BE1004202A5 (fr) 1992-10-13
SE8901031L (sv) 1989-09-25
AU2990492A (en) 1993-03-04
LU87489A1 (fr) 1989-10-26
IT8919917A0 (it) 1989-03-24
KR890014743A (ko) 1989-10-25
PT90118A (pt) 1989-11-10
JPH0279981A (ja) 1990-03-20
NO891273D0 (no) 1989-03-22
NZ228427A (en) 1993-03-26
GB2219799A (en) 1989-12-20
CH681081A5 (es) 1993-01-15
GR890100185A (en) 1990-01-19
HUT50501A (en) 1990-02-28
IE890950L (en) 1989-09-24
DE3910084A1 (de) 1989-11-16
GB8906758D0 (en) 1989-05-10
FR2632321A1 (fr) 1989-12-08
NL8900724A (nl) 1989-10-16
DK146789A (da) 1989-09-25
FI891308A0 (fi) 1989-03-20
IL89673A0 (en) 1989-09-28
GB2219799B (en) 1992-10-14
FI891308L (fi) 1989-09-25
DK146789D0 (da) 1989-03-22
GR1000876B (el) 1993-03-16
PT90118B (pt) 1994-07-29
FI891308A7 (fi) 1989-09-25
IT1234961B (it) 1992-06-09

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