ES2017122A6 - Procedimiento de preparacion de proteina de ligado de heparina. - Google Patents

Procedimiento de preparacion de proteina de ligado de heparina.

Info

Publication number
ES2017122A6
ES2017122A6 ES8901356A ES8901356A ES2017122A6 ES 2017122 A6 ES2017122 A6 ES 2017122A6 ES 8901356 A ES8901356 A ES 8901356A ES 8901356 A ES8901356 A ES 8901356A ES 2017122 A6 ES2017122 A6 ES 2017122A6
Authority
ES
Spain
Prior art keywords
heparin
processes
producing
well
binding proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8901356A
Other languages
English (en)
Inventor
Hans Flodgaard
Erik Ostergaard
Johannes Thomsen
Stephen Bayne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nordisk Gentofte AS
Original Assignee
Nordisk Gentofte AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK145388A external-priority patent/DK145388D0/da
Priority claimed from DK73789A external-priority patent/DK73789D0/da
Application filed by Nordisk Gentofte AS filed Critical Nordisk Gentofte AS
Publication of ES2017122A6 publication Critical patent/ES2017122A6/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/515—Angiogenesic factors; Angiogenin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Vascular Medicine (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO DE PREPARACION DE PROTEINA DE LIGADO DE HEPARINA, QUE TIENE, EN ESTADO GLICOSILADO, UN PESO MOLECULAR APARENTE DE APROXIMADAMENTE 28 KDA, DETERMINADO POR ELECTROFORESIS EN GEL DE POLIACRILAMIDA DE SOLUCION DE DODECILSULFONATO SODICO (SDS-PAGE), BASADO EN LA EXTRACCION DE TROMBOCITOS Y LA PURIFICACION DEL EXTRACTO DE CROMATOGRAFIA SOBRE HEPARINA-SEFAROSA Y CROMATOGRAFIA LIQUIDA DE ALTO RENDIMIENTO (HPLC) DE FASE INVERSA. TAL METODO CROMATOGRAFICO COMPRENDE ELUCION EN GRADIENTE CON NACL DED 0,5 M HASTA 3 M DE UNA COLUMNA A TRAVES DE LA CUAL SE HA VERTIDO PRIMERAMENTE UN EXTRACTO DE LAS PLAQUETAS SANGUINEAS. LA PROTEINA OBTENIDA PRESENTA PROPIEDADES ANGIOGENAS IN VIVO Y SE APLICA A LA PREPARACION DE FARMACOS PARA EL TRATAMIENTO TOPICO DE LESIONES EXTERNAS EN MAMIFEROS.
ES8901356A 1988-03-17 1989-03-17 Procedimiento de preparacion de proteina de ligado de heparina. Expired - Lifetime ES2017122A6 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK145388A DK145388D0 (da) 1988-03-17 1988-03-17 Heparin-bindende protein
DK73789A DK73789D0 (da) 1989-02-17 1989-02-17 Heparin-bindende protein

Publications (1)

Publication Number Publication Date
ES2017122A6 true ES2017122A6 (es) 1991-01-01

Family

ID=26064553

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8901356A Expired - Lifetime ES2017122A6 (es) 1988-03-17 1989-03-17 Procedimiento de preparacion de proteina de ligado de heparina.

Country Status (12)

Country Link
EP (1) EP0409858B1 (es)
JP (1) JP2846385B2 (es)
AU (1) AU617795B2 (es)
DE (1) DE68911297T2 (es)
ES (1) ES2017122A6 (es)
FI (1) FI101626B1 (es)
HU (1) HU219883B (es)
IE (1) IE61947B1 (es)
IL (1) IL89661A (es)
NO (1) NO179588C (es)
NZ (2) NZ228370A (es)
WO (1) WO1989008666A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL92816A0 (en) * 1988-12-22 1990-09-17 Biogrowth Inc Recombinant dna molecules,hosts,processes and human somatomedin carrier protein-like polypeptides
US7026291B1 (en) 1989-01-31 2006-04-11 The United States Of America As Represented By The Department Of Health And Human Services Epithelial cell specific growth factor, keratinocyte growth factor (KGF)
GEP20022681B (en) 1989-01-31 2002-04-25 Aaronson Us Stuart A Protein KFG, Antibodies, Pharmaceutical Composition, Their Use, recombinant DNA, Cell, Method for Discoveries and Production of Proteins, Method for Stimulation of Epithelial Cells Growth
US5198423A (en) * 1989-05-26 1993-03-30 Takara Shuzo Co., Ltd. Functional polypeptide containing a cell binding domain and a heparin binding domain of fibronectin
US5607916A (en) * 1989-07-05 1997-03-04 The Board Of Regents Of The University Of Oklahoma Method and composition for the treatment of septic shock
ATE199095T1 (de) * 1990-10-16 2001-02-15 Childrens Medical Center Heparin bindendes mitogen mit homologie zum epidermis wachstumsfaktor (egf)
DK261490D0 (da) * 1990-10-31 1990-10-31 Novo Nordisk As New pharmaceutical compound
WO1993005396A1 (en) * 1991-09-12 1993-03-18 Novo Nordisk A/S A method of screening for inhibitors of heparin-binding protein
RU2200573C2 (ru) * 1994-04-21 2003-03-20 Леукотех А/С Связывающий гепарин белок для лечения сепсиса
US5939390A (en) * 1995-03-09 1999-08-17 Novo Nordisk A/S Pharmaceutical composition
EP0871721A1 (en) * 1995-03-09 1998-10-21 Novo Nordisk A/S A protein-lipid conjugate, consisting of heparin binding protein (hbp) and a ceramide analogue, and its pharmaceutical use in treatment of conditions involving stress injury to cells
WO1998038214A1 (en) * 1997-02-28 1998-09-03 The Texas A & M University System Heparan sulfate/heparin interacting protein compositions and methods of use
EA200101093A1 (ru) * 1999-04-29 2002-04-25 Лейкотек А/С Экспрессия гепарин-связывающего протеина в рекомбинантных клетках млекопитающих
AU2003226907A1 (en) * 2002-03-27 2003-10-08 Leukotech A/S Method for the preparation of recombinant mammalian heparin-binding protein (hbp)
JPWO2006036002A1 (ja) * 2004-09-28 2008-05-15 学校法人 久留米大学 新規骨転移マーカーペプチドおよびそれを用いた骨転移の診断方法
CN112647358A (zh) * 2020-12-07 2021-04-13 静宁县恒达有限责任公司 一种磷硼杂链预聚物嵌段聚氨酯水性防潮阻燃纸箱覆膜剂及其制备方法与应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000198A1 (en) * 1987-07-07 1989-01-12 Biotechnology Research Associates, J.V. Recombinant fibroblast growth factors
EP0241136A3 (en) * 1986-03-07 1989-12-27 The President And Fellows Of Harvard College Human class 1 heparin-binding growth factor
US4883751A (en) * 1986-05-28 1989-11-28 New York University Specific immunoassay for heparin
WO1988010269A1 (en) * 1987-06-18 1988-12-29 Monash University Growth factors

Also Published As

Publication number Publication date
IL89661A (en) 1999-05-09
FI101626B (fi) 1998-07-31
NO179588B (no) 1996-07-29
NO179588C (no) 1996-11-06
FI904546A0 (fi) 1990-09-14
NZ243844A (en) 1994-01-26
NO904024L (no) 1990-11-13
IL89661A0 (en) 1989-09-28
IE890864L (en) 1989-09-17
AU3346289A (en) 1989-10-05
HU892118D0 (en) 1991-05-28
DE68911297T2 (de) 1994-03-31
JP2846385B2 (ja) 1999-01-13
NO904024D0 (no) 1990-09-14
NZ228370A (en) 1994-01-26
IE61947B1 (en) 1994-11-30
HU219883B (hu) 2001-08-28
EP0409858B1 (en) 1993-12-08
FI101626B1 (fi) 1998-07-31
WO1989008666A1 (en) 1989-09-21
AU617795B2 (en) 1991-12-05
HUT58107A (en) 1992-01-28
DE68911297D1 (de) 1994-01-20
EP0409858A1 (en) 1991-01-30
JPH03504919A (ja) 1991-10-31

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Legal Events

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