ES2038965T3 - Procedimiento y dispositivo para el cultivo de celulas. - Google Patents

Procedimiento y dispositivo para el cultivo de celulas.

Info

Publication number
ES2038965T3
ES2038965T3 ES198686116036T ES86116036T ES2038965T3 ES 2038965 T3 ES2038965 T3 ES 2038965T3 ES 198686116036 T ES198686116036 T ES 198686116036T ES 86116036 T ES86116036 T ES 86116036T ES 2038965 T3 ES2038965 T3 ES 2038965T3
Authority
ES
Spain
Prior art keywords
nutritive
media
membrane
cultivation
substances
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686116036T
Other languages
English (en)
Inventor
Michael Dr. Kearns
Michael James Dr. Rer. Nat. Comer
Ulrich Steegmans
Herbert Dr. Med. Jungfer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2038965T3 publication Critical patent/ES2038965T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers

Landscapes

  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA CULTIVAR CELULAS, EN EL QUE UN CULTIVO CELULAR, EN UN REACTOR, SE MANTIENE EN SUSPENSION MUY HOMOGENEA Y EN CONDICIONES AMBIENTALES CONTROLADAS, EN UN MEDIO DE CULTIVO, MEDIANTE UN DISPOSITIVO DE AGITACION, SE INTRODUCEN SUSTANCIAS NUTRITIVAS PARA LAS CELULAS Y SE RETIRAN PRODUCTOS DE DESECHO DE LAS CELULAS. SE CONSIGUE UNA DENSIDAD DE CELULAS ESPECIALMENTE ALTA Y UN RENDIMIENTO DE PRODUCTO ESPECIALMENTE ALTO, MEDIANTE LA COMBINACION DE LAS SIGUIENTES MEDIDAS: SE UTILIZA UN MEDIO NUTRITIVO SEPARADO DEL MEDIO DE CULTIVO, QUE FLUYE POR UNA VIA DE CIRCULACION SEPARADA DEL MEDIO DE CULTIVO POR UNA MEMBRANA SEMIPERMEABLE, HABIENDO SIDO PROYECTADA LA MEMBRANA DE TAL MANERA QUE SEA PERMEABLE PARA LAS SUSTANCIAS NUTRITIVAS Y PARA LOS PRODUCTOS DE DESECHO DE LAS CELULAS, PERO IMPERMEABLE A LOS COMPONENTES DEL MEDIO DE CULTIVO DE PESO MOLECULAR MAS ALTO, Y HACIENDO CIRCULAR POR UN LADO DE LA MEMBRANA EL MEDIO DE CULTIVO CON LAS CELULAS Y, POR EL OTRO LADO DE LA MEMBRANA, EL MEDIONUTRITIVO QUE CONTIENE LAS SUSTANCIAS NUTRITIVAS, DE TAL MANERA QUE LAS SUSTANCIAS NUTRITIVAS PASEN A TRAVES DE LA MEMBRANA DESDE EL MEDIO NUTRITIVO AL MEDIO DE CULTIVO Y LAS SUSTANCIAS DE DESECHO PASEN AL MEDIO NUTRITIVO DESDE EL MEDIO DE CULTIVO.
ES198686116036T 1985-11-26 1986-11-20 Procedimiento y dispositivo para el cultivo de celulas. Expired - Lifetime ES2038965T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853541738 DE3541738A1 (de) 1985-11-26 1985-11-26 Verfahren und vorrichtung zum kultivieren von zellen

Publications (1)

Publication Number Publication Date
ES2038965T3 true ES2038965T3 (es) 1993-08-16

Family

ID=6286863

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686116036T Expired - Lifetime ES2038965T3 (es) 1985-11-26 1986-11-20 Procedimiento y dispositivo para el cultivo de celulas.

Country Status (5)

Country Link
EP (1) EP0224800B1 (es)
JP (1) JPH0740928B2 (es)
AT (1) ATE85357T1 (es)
DE (2) DE3541738A1 (es)
ES (1) ES2038965T3 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5656421A (en) * 1990-02-15 1997-08-12 Unisyn Technologies, Inc. Multi-bioreactor hollow fiber cell propagation system and method
NO174588C (no) * 1992-01-22 1994-06-01 Norske Meierier Fremgangsmåte for kontinuerlig fremstilling av ulike biotekniske produktgrupper ved anvendelse av kontinuerlig membranreaktor
JP2002239077A (ja) * 2001-02-13 2002-08-27 Newgin Corp 遊技機
DE10120835C2 (de) * 2001-04-27 2003-04-10 Sifin Inst Fuer Immunpraeparat Verfahren zur Herstellung von monoklonalen Antikörpern
ES2295418T3 (es) * 2002-09-16 2008-04-16 Pan-Biotech Gmbh Equipo para el cultivo de celulas, en especial de celulas humanas o de animales.
ES2295465T3 (es) 2002-09-16 2008-04-16 Pan-Biotech Gmbh Procedimiento para el cultivo de celulas, en especial de celulas humanas o de animales.
DE10249959B4 (de) * 2002-10-26 2007-05-16 Fraunhofer Ges Forschung Verfahren und Vorrichtung zur Herstellung von Essigsäure
JP2010178734A (ja) * 2009-01-08 2010-08-19 Ihi Corp 撹拌装置
PL2437874T3 (pl) * 2009-06-05 2015-10-30 Hoffmann La Roche Urządzenie do hodowli komórek
DE102010005415B4 (de) 2010-01-22 2015-07-16 Zellwerk Gmbh Verfahren und Vorrichtung zur dynamischen Expansion und/oder Differenzierung von suspendierten primären Zellen oder Stammzellen humanen und tierischen Ursprungs
US20120301923A1 (en) 2011-05-27 2012-11-29 Roche Diagnostics Operations, Inc. Dialysis fermenter-bioreactor with dialysis device
EP2843036A4 (en) * 2012-04-27 2015-04-22 Asahi Chemical Ind CELL CULTURE SYSTEM AND CELL CULTURE PROCESS

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2238759A1 (en) * 1973-07-23 1975-02-21 Milk Marketing Board Bacterial cell concentrates - for inoculating milk or starter solns in cheese mfr. produced by diffusion fermentation
JPS5031091A (es) * 1973-07-26 1975-03-27
DE3016636A1 (de) * 1980-04-30 1981-11-05 Boehringer Mannheim Gmbh, 6800 Mannheim Stoffaustauschmodul, insbesondere fuer medizinische anwendungen
ZA839242B (en) * 1982-12-15 1984-09-26 Bio Response Inc A method and system for culturing and treating substances disposed in a flowing culture fluid
JPS60207581A (ja) * 1984-03-30 1985-10-19 Teijin Ltd 細胞培養方法

Also Published As

Publication number Publication date
DE3541738A1 (de) 1987-05-27
EP0224800B1 (de) 1993-02-03
JPH0740928B2 (ja) 1995-05-10
JPS62130683A (ja) 1987-06-12
ATE85357T1 (de) 1993-02-15
DE3687699D1 (de) 1993-03-18
EP0224800A3 (en) 1988-08-31
EP0224800A2 (de) 1987-06-10

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