ES2045113T3 - Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado. - Google Patents

Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado.

Info

Publication number
ES2045113T3
ES2045113T3 ES88303904T ES88303904T ES2045113T3 ES 2045113 T3 ES2045113 T3 ES 2045113T3 ES 88303904 T ES88303904 T ES 88303904T ES 88303904 T ES88303904 T ES 88303904T ES 2045113 T3 ES2045113 T3 ES 2045113T3
Authority
ES
Spain
Prior art keywords
antibody
obtaining
antigen
sandwiched
standard curve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88303904T
Other languages
English (en)
Inventor
Ker-Kong Tung
Linda Katherine Cragle
Frederick William Rood
Shih-Yun Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Application granted granted Critical
Publication of ES2045113T3 publication Critical patent/ES2045113T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/801—Electron dense compounds, e.g. ferritin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/803—Stabe free radicals, e.g. spin immunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/82—Hepatitis associated antigens and antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/827—Lectins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UN METODO PARA OBTENER UNA CURVA PATRON LINEAL REAL EN UN TIPO DE INMUNOENSAYO DE INTERCALACION, DONDE UN PRIMER ANTICUERPO UNIDO A UN SOPORTE INSOLUBLE Y UN SEGUNDO ANTICUERPO MARCADO NO ENLAZADO FORMAN COMPLEJO CON EL ANTIGENO CONTENIDO EN UNA MUESTRA DE ENSAYO PARA FORMAR UN COMPLEJO ANTICUERPO-ANTIGENO-ANTICUERPO MARCADO, QUE LUEGO SE DETECTA. SE AÑADEN A LA MEZCLA DE REACCION EL PRIMER ANTICUERPO NO MARCADO NO ENLAZADO Y/O EL SEGUNDO ANTICUERPO NO MARACADO NO ENLAZADO PARA RETIRAR EL EXCESO DE ANTIGENO DEL COMPLEJO PRODUCTO FINAL DESEADO, HACIENDO ASI AL ANTIGENO DE INTERES EL FACTOR LIMITANTE DE LA VELOCIDAD EN LA INMUNOREACCION TOTAL. ESTO DA LUGAR A UNA SEUDOREACCION DE PRIMER ORDEN QUE PRODUCE UNA CURVA PATRON LINEAL REAL.
ES88303904T 1987-04-30 1988-04-29 Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado. Expired - Lifetime ES2045113T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/044,099 US4788138A (en) 1987-04-30 1987-04-30 Method to achieve a linear standard curve in a sandwich immunoassay

Publications (1)

Publication Number Publication Date
ES2045113T3 true ES2045113T3 (es) 1994-01-16

Family

ID=21930530

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88303904T Expired - Lifetime ES2045113T3 (es) 1987-04-30 1988-04-29 Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado.

Country Status (11)

Country Link
US (1) US4788138A (es)
EP (1) EP0289335B1 (es)
JP (1) JP2568910B2 (es)
AT (1) ATE84879T1 (es)
AU (1) AU617959B2 (es)
CA (1) CA1294870C (es)
DE (1) DE3877617T2 (es)
ES (1) ES2045113T3 (es)
FI (1) FI90799C (es)
NO (1) NO175657C (es)
WO (1) WO1988008535A1 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4891313A (en) * 1988-01-21 1990-01-02 Boehringer Manheim Corporation Method for determination of a component of a sample
WO1989010974A1 (en) * 1988-05-11 1989-11-16 Trustees Of The Sisters Of Charity Of Australia Enzyme immunoassay system
GB8812213D0 (en) * 1988-05-24 1988-06-29 Ares Serono Res & Dev Ltd Method of assay
JPH0754322B2 (ja) * 1988-11-07 1995-06-07 三洋化成工業株式会社 免疫学的定量法および検査用試薬
US5939272A (en) * 1989-01-10 1999-08-17 Biosite Diagnostics Incorporated Non-competitive threshold ligand-receptor assays
FR2652900B1 (fr) * 1989-10-11 1994-01-28 Clonatec Sa Procede d'analyse immunologique de type "sandwich" son application au depistage prenatal in vitro de la trisomie 21 et necessaire pour sa mise en óoeuvre.
FR2657167B1 (fr) * 1990-01-16 1992-05-07 Clonatec Sa Procede de depistage prenatal in vitro de la trisomie 21 fóoetale dans un echantillon biologique susceptible de contenir de l'hcg et necessaire pour sa mise en óoeuvre.
TW274582B (es) * 1992-08-03 1996-04-21 Hoffmann La Roche
US5556759A (en) * 1993-08-02 1996-09-17 Beach; Peter G. Assay and method for determining newborn risk for sudden infant death syndrome
US5597700A (en) * 1994-04-28 1997-01-28 California Research, Llc Method for detecting free insulin-like growth-factor-binding protein 1 and a test device for detecting the ruptures of fetal membranes using the above method
DE69530043T2 (de) * 1994-11-08 2003-10-16 Tosoh Corp., Shinnanyo Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung
US5705353A (en) * 1995-06-07 1998-01-06 Beckman Instruments, Inc. Method of reducing interferences in assays
US6686165B2 (en) * 1997-05-20 2004-02-03 Erasmus Universiteit Rotterdam Recognition of tumor-specific gene products in cancer
US6284472B1 (en) 1998-10-05 2001-09-04 Dade Behring Inc. Method for extending the range of an immunoassay
US6905886B2 (en) * 2000-08-11 2005-06-14 Quest Diagnostics Investments Incorporated Preservative solutions
US6387711B1 (en) * 2000-08-11 2002-05-14 Quest Diagnostics Investments Incorporated Liquid intact parathyroid hormone (PTH) standards
US20060292558A1 (en) * 2004-07-19 2006-12-28 Cell Biosciences Inc. Methods and apparatus for protein assay diagnostics
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
WO2006014680A1 (en) * 2004-07-19 2006-02-09 Cell Biosciences, Inc. Methods and devices for analyte detection
US7846676B2 (en) * 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
US8945361B2 (en) 2005-09-20 2015-02-03 ProteinSimple Electrophoresis standards, methods and kits
US20080017512A1 (en) * 2006-07-24 2008-01-24 Bordunov Andrei V Coatings for capillaries capable of capturing analytes
US8956823B2 (en) * 2007-08-20 2015-02-17 Bio-Rad Laboratories, Inc. Anti-antibody reagent
WO2009126336A1 (en) * 2008-04-11 2009-10-15 Becton, Dickinson And Company Methods of controlling the sensitivity and dynamic range of a homogeneous assay
US9804079B2 (en) 2012-04-19 2017-10-31 ProteinSimple Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
JP6277099B2 (ja) 2014-09-09 2018-02-07 富士フイルム株式会社 試薬キット、測定キット及び被検物質の測定方法。
CN111693690A (zh) * 2020-07-05 2020-09-22 江苏拜明生物技术有限公司 改善一步法双抗夹心反应中hook效应及平台期的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4016143A (en) * 1971-12-07 1977-04-05 Bayer Aktiengesellschaft Polyurethanes based on aromatic polyamines
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
EP0101228B1 (en) * 1982-08-06 1986-10-08 International Institute Of Cellular And Molecular Pathology (Icp) Particle agglutination assay of antigens
US4659678A (en) * 1982-09-29 1987-04-21 Serono Diagnostics Limited Immunoassay of antigens
EP0149602A1 (en) * 1983-07-25 1985-07-31 Beckman Instruments, Inc. Immunometric assay using polyclonal and monoclonal antibodies and a kit for use therein
US4595661A (en) * 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4690890A (en) * 1984-04-04 1987-09-01 Cetus Corporation Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay
US4743542A (en) * 1985-04-11 1988-05-10 Ortho Diagnostic Method for forestalling the hook effect in a multi-ligand immunoassay system

Also Published As

Publication number Publication date
AU1981288A (en) 1988-12-02
ATE84879T1 (de) 1993-02-15
NO885808D0 (no) 1988-12-29
AU617959B2 (en) 1991-12-05
DE3877617T2 (de) 1993-05-13
FI90799B (fi) 1993-12-15
EP0289335A3 (en) 1988-12-21
NO175657C (no) 1994-11-09
NO885808L (no) 1988-12-29
WO1988008535A1 (en) 1988-11-03
FI90799C (fi) 1994-03-25
EP0289335B1 (en) 1993-01-20
JPH01503177A (ja) 1989-10-26
US4788138A (en) 1988-11-29
CA1294870C (en) 1992-01-28
EP0289335A2 (en) 1988-11-02
JP2568910B2 (ja) 1997-01-08
NO175657B (es) 1994-08-01
DE3877617D1 (de) 1993-03-04
FI886000L (fi) 1988-12-28

Similar Documents

Publication Publication Date Title
ES2045113T3 (es) Un metodo para obtener una curva patron lineal en un inmunoensayo de tipo emparedado.
ES2009368A6 (es) Enziomoinmunoensayo para detectar antigenos de hiv en suero humano.
NO881151L (no) Syntetisk hiv-1-antigen.
ES2082777T3 (es) Un metodo para la deteccion de un anti-cuerpo y antigeno en un inmunoensayo.
SE7413484L (es)
ES2114727T3 (es) Inmunoensayo rapido para deteccion de anticuerpos o antigenos que incorpora extraccion de la muestra y reaccion inmunogena simultaneas.
FI84107C (fi) HbA1c:s immunologiska bestaemningsfoerfarande som innefattar denaturering
ES8206856A1 (es) Mejoras en un metodo y su correspondiente equipo de ensayo para determinar antigenos por medio de una reaccion inmuno- quimica
ATE152244T1 (de) Verfahren zur herstellung von imunodiagnose- mitteln
NO821431L (no) Fremgangsmaate og sett for proeving av antilegemer mot antigener
ES2076948T3 (es) Inmunoensayo sobre una superficie solida prebloqueada.
ATE2699T1 (de) Serologisches verfahren zum nachweis von neisseria gonorrhoeae antikoerpern im menschlichen serum, ein dazu angewendetes speziesspezifisches waermestabiles antigen und verfahren zur herstellung dieses antigens.
ES2040901T3 (es) Velo de soporte para reactivos impregnados de modo desprendible.
SE7714034L (sv) Forfarande for enzymkopplad immunoanalys
BR9507389A (pt) Método para detectar a presença de uma espécie mycobacterium e um kit e anticorpos para uso no mesmo
KR900004935A (ko) 슈도 모나즈 아에루기노사의 지질단백질 i(ompi)
GB2008247A (en) Detecting or quantifying substances using labelling techniques
ES2080955T3 (es) Anticuerpos especificos de la pancreaelastasa 1, procedimiento para su obtencion y equipo de analisis que contiene este tipo de anticuerpos.
ES2131279T3 (es) Inmunoensayo de tipo sandwich para n-peptido.
DE69733044D1 (de) Eine Methode zur Messung der Konzentration eines Antigens
ES8604693A1 (es) Un metodo para detectar un anticuerpo o un antigeno contenido en una muestra de fluido de origen biologico.
ES2061664T3 (es) Anticuerpos monoclonales que reconocen a alfa-hanp y correspondientes hibridomas, su preparacion y uso.
FR2277564A1 (fr) Procede de dosage immunologique utilisant un systeme heterologue d'anticorps ou d'antigenes
ES8706445A1 (es) Un metodo en emparedado de doble anticuerpo para detectar o ensayar un antigeno seleccionado de urocinasa humana y un precursor de la misma
ES2121756T3 (es) Analisis enzimatico y equipo de analisis para medir la activacion celular.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 289335

Country of ref document: ES